Restriction map for
pF1KB3191
(Linear) (Six-base) MAPSORT of: KIBB3191.seq Check: 970 from: 1 to: 1767
KIBB3191.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AatII G_ACGT'C
Cuts at: 0 1724 1767
Size: 1724 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1138 1417 1767
Size: 776 362 279 350
Fragments arranged by size:
776 362 350 279
AflIII A'CryG_T
Cuts at: 0 1017 1767
Size: 1017 750
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1534 1767
Size: 1534 233
AlwNI CAG_nnn'CTG
Cuts at: 0 702 867 1209 1767
Size: 702 165 342 558
Fragments arranged by size:
702 558 342 165
ApaI G_GGCC'C
Cuts at: 0 1434 1646 1767
Size: 1434 212 121
Fragments arranged by size:
1434 212 121
ApaLI G'TGCA_C
Cuts at: 0 256 1767
Size: 256 1511
AvaI C'yCGr_G
Cuts at: 0 467 1767
Size: 467 1300
BanII G_rGCy'C
Cuts at: 0 809 1434 1646 1767
Size: 809 625 212 121
Fragments arranged by size:
809 625 212 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 764 1767
Size: 764 1003
BbvCI CC'TCA_GC
Cuts at: 0 1235 1767
Size: 1235 532
BclI T'GATC_A
Cuts at: 0 962 1767
Size: 962 805
BglI GCCn_nnn'nGGC
Cuts at: 0 1741 1767
Size: 1741 26
BglII A'GATC_T
Cuts at: 0 974 1767
Size: 974 793
BlpI GC'TnA_GC
Cuts at: 0 1112 1767
Size: 1112 655
Bme1580I G_kGCm'C
Cuts at: 0 260 1132 1434 1646 1767
Size: 260 872 302 212 121
Fragments arranged by size:
872 302 260 212 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 1197 1767
Size: 1197 570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 669 1767
Size: 669 1098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 391 607 739 793 1767
Size: 298 93 216 132 54 974
Fragments arranged by size:
974 298 216 132 93 54
Bpu10I CC'TnA_GC
Cuts at: 0 1235 1767
Size: 1235 532
BsaI GGTCTCn'nnnn_
Cuts at: 0 1057 1466 1767
Size: 1057 409 301
Fragments arranged by size:
1057 409 301
BsaAI yAC'GTr
Cuts at: 0 255 1767
Size: 255 1512
BsaBI GATnn'nnATC
Cuts at: 0 1186 1767
Size: 1186 581
BsaHI Gr'CG_yC
Cuts at: 0 1241 1721 1767
Size: 1241 480 46
Fragments arranged by size:
1241 480 46
BsaWI w'CCGG_w
Cuts at: 0 509 1468 1767
Size: 509 959 299
Fragments arranged by size:
959 509 299
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 985 1015 1767
Size: 985 30 752
Fragments arranged by size:
985 752 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 1767
Size: 313 1454
BseYI C'CCAG_C
Cuts at: 0 216 564 695 1299 1585 1767
Size: 216 348 131 604 286 182
Fragments arranged by size:
604 348 286 216 182 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1471 1767
Size: 1471 296
BsiEI CG_ry'CG
Cuts at: 0 11 1767
Size: 11 1756
BsiHKAI G_wGCw'C
Cuts at: 0 47 260 356 523 809 1091 1767
Size: 47 213 96 167 286 282 676
Fragments arranged by size:
676 286 282 213 167 96 47
Bsp1286I G_dGCh'C
Cuts at: 0 47 260 356 523 809 1091 1132 1434
Size: 47 213 96 167 286 282 41 302
Cuts at: 1434 1646 1767
Size: 212 121
Fragments arranged by size:
302 286 282 213 212 167 121 96
47 41
BspEI T'CCGG_A
Cuts at: 0 509 1468 1767
Size: 509 959 299
Fragments arranged by size:
959 509 299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1493 1767
Size: 1493 274
BsrDI GCAATG_nn'
Cuts at: 0 69 1767
Size: 69 1698
BssSI C'ACGA_G
Cuts at: 0 349 1767
Size: 349 1418
BstAPI GCAn_nnn'nTGC
Cuts at: 0 264 384 1095 1767
Size: 264 120 711 672
Fragments arranged by size:
711 672 264 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 585 1458 1767
Size: 117 468 873 309
Fragments arranged by size:
873 468 309 117
BstYI r'GATC_y
Cuts at: 0 714 974 1767
Size: 714 260 793
Fragments arranged by size:
793 714 260
Bsu36I CC'TnA_GG
Cuts at: 0 1133 1767
Size: 1133 634
BtgI C'CryG_G
Cuts at: 0 536 1568 1767
Size: 536 1032 199
Fragments arranged by size:
1032 536 199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 21 96 675 1767
Size: 21 75 579 1092
Fragments arranged by size:
1092 579 75 21
BtsI GCAGTG_nn'
Cuts at: 0 258 329 824 1767
Size: 258 71 495 943
Fragments arranged by size:
943 495 258 71
DraIII CAC_nnn'GTG
Cuts at: 0 384 1767
Size: 384 1383
DrdI GACnn_nn'nnGTC
Cuts at: 0 1755 1767
Size: 1755 12
EaeI y'GGCC_r
Cuts at: 0 8 287 1262 1460 1619 1767
Size: 8 279 975 198 159 148
Fragments arranged by size:
975 279 198 159 148 8
EagI C'GGCC_G
Cuts at: 0 8 1767
Size: 8 1759
EarI CTCTTCn'nnn_
Cuts at: 0 454 1108 1510 1767
Size: 454 654 402 257
Fragments arranged by size:
654 454 402 257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 505 1767
Size: 505 1262
EcoICRI GAG'CTC
Cuts at: 0 807 1767
Size: 807 960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 391 607 739 776 793 1138 1417
Size: 298 93 216 132 37 17 345 279
Cuts at: 1417 1767
Size: 350
Fragments arranged by size:
350 345 298 279 216 132 93 37
17
EcoO109I rG'GnC_Cy
Cuts at: 0 137 192 723 823 1590 1642 1767
Size: 137 55 531 100 767 52 125
Fragments arranged by size:
767 531 137 125 100 55 52
FspI TGC'GCA
Cuts at: 0 1579 1767
Size: 1579 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 723 755 985 1017 1767
Size: 723 32 230 32 750
Fragments arranged by size:
750 723 230 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1767
Size: 653 1114
MscI TGG'CCA
Cuts at: 0 289 1264 1462 1621 1767
Size: 289 975 198 159 146
Fragments arranged by size:
975 289 198 159 146
MslI CAynn'nnrTG
Cuts at: 0 40 535 619 1364 1516 1767
Size: 40 495 84 745 152 251
Fragments arranged by size:
745 495 251 152 84 40
MspA1I CmG'CkG
Cuts at: 0 216 276 754 943 1116 1197 1299 1767
Size: 216 60 478 189 173 81 102 468
Fragments arranged by size:
478 468 216 189 173 102 81 60
NcoI C'CATG_G
Cuts at: 0 536 1767
Size: 536 1231
NspI r_CATG'y
Cuts at: 0 1021 1281 1563 1767
Size: 1021 260 282 204
Fragments arranged by size:
1021 282 260 204
PciI A'CATG_T
Cuts at: 0 1017 1767
Size: 1017 750
PflMI CCAn_nnn'nTGG
Cuts at: 0 1716 1767
Size: 1716 51
PfoI T'CCnGG_A
Cuts at: 0 1649 1767
Size: 1649 118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 1138 1170 1767
Size: 505 32 601 32 597
Fragments arranged by size:
601 597 505 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 137 192 1590 1767
Size: 137 55 1398 177
Fragments arranged by size:
1398 177 137 55
PspOMI G'GGCC_C
Cuts at: 0 1430 1642 1767
Size: 1430 212 125
Fragments arranged by size:
1430 212 125
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 213 245 1767
Size: 213 32 1522
Fragments arranged by size:
1522 213 32
PstI C_TGCA'G
Cuts at: 0 1634 1767
Size: 1634 133
PvuII CAG'CTG
Cuts at: 0 216 943 1116 1197 1299 1767
Size: 216 727 173 81 102 468
Fragments arranged by size:
727 468 216 173 102 81
SacI G_AGCT'C
Cuts at: 0 809 1767
Size: 809 958
SanDI GG'GwC_CC
Cuts at: 0 1590 1767
Size: 1590 177
SapI GCTCTTCn'nnn_
Cuts at: 0 1510 1767
Size: 1510 257
ScaI AGT'ACT
Cuts at: 0 505 1767
Size: 505 1262
SexAI A'CCwGG_T
Cuts at: 0 845 1767
Size: 845 922
SfcI C'TryA_G
Cuts at: 0 1630 1767
Size: 1630 137
StyI C'CwwG_G
Cuts at: 0 116 123 536 578 1451 1767
Size: 116 7 413 42 873 316
Fragments arranged by size:
873 413 316 116 42 7
TatI w'GTAC_w
Cuts at: 0 503 1274 1767
Size: 503 771 493
Fragments arranged by size:
771 503 493
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 129 161 1231 1263 1767
Size: 129 32 1070 32 504
Fragments arranged by size:
1070 504 129 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 598 847 1767
Size: 598 249 920
Fragments arranged by size:
920 598 249
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 843 1227 1767
Size: 114 729 384 540
Fragments arranged by size:
729 540 384 114
ZraI GAC'GTC
Cuts at: 0 1722 1767
Size: 1722 45
Enzymes that do cut:
AatII AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI
BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrDI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FspI Hin4I MmeI MscI
MslI MspA1I NcoI NspI PciI PflMI PfoI PpiI
PpuMI PspOMI PsrI PstI PvuII SacI SanDI SapI
ScaI SexAI SfcI StyI TatI TstI Tth111I XcmI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BfrBI BmgBI BmtI
BpuEI BsiWI BsmI BsmBI BspHI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I ClaI CspCI DraI EcoNI
EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PshAI PsiI PspXI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII XbaI
XhoI XmaI XmnI