Restriction map for
pF1KB3144
(Linear) (Six-base) MAPSORT of: KIBB3144.seq Check: 2903 from: 1 to: 1725
KIBB3144.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 447 578 582 1725
Size: 447 131 4 1143
Fragments arranged by size:
1143 447 131 4
AclI AA'CG_TT
Cuts at: 0 1426 1725
Size: 1426 299
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1643 1689 1725
Size: 1186 457 46 36
Fragments arranged by size:
1186 457 46 36
AfeI AGC'GCT
Cuts at: 0 1213 1725
Size: 1213 512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1493 1725
Size: 1493 232
AlwNI CAG_nnn'CTG
Cuts at: 0 606 681 1725
Size: 606 75 1044
Fragments arranged by size:
1044 606 75
ApaI G_GGCC'C
Cuts at: 0 55 369 626 665 1725
Size: 55 314 257 39 1060
Fragments arranged by size:
1060 314 257 55 39
ApoI r'AATT_y
Cuts at: 0 1277 1725
Size: 1277 448
AscI GG'CGCG_CC
Cuts at: 0 580 1725
Size: 580 1145
AvaI C'yCGr_G
Cuts at: 0 23 56 619 1725
Size: 23 33 563 1106
Fragments arranged by size:
1106 563 33 23
BanI G'GyrC_C
Cuts at: 0 513 856 1156 1615 1725
Size: 513 343 300 459 110
Fragments arranged by size:
513 459 343 300 110
BanII G_rGCy'C
Cuts at: 0 55 200 301 369 626 665 1725
Size: 55 145 101 68 257 39 1060
Fragments arranged by size:
1060 257 145 101 68 55 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 278 1725
Size: 278 1447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 324 358 1725
Size: 324 34 1367
Fragments arranged by size:
1367 324 34
BglI GCCn_nnn'nGGC
Cuts at: 0 100 415 694 719 1725
Size: 100 315 279 25 1006
Fragments arranged by size:
1006 315 279 100 25
BglII A'GATC_T
Cuts at: 0 995 1725
Size: 995 730
BlpI GC'TnA_GC
Cuts at: 0 104 112 838 971 1725
Size: 104 8 726 133 754
Fragments arranged by size:
754 726 133 104 8
Bme1580I G_kGCm'C
Cuts at: 0 55 369 518 626 665 675 1111 1159
Size: 55 314 149 108 39 10 436 48
Cuts at: 1159 1620 1725
Size: 461 105
Fragments arranged by size:
461 436 314 149 108 105 55 48
39 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 422 1114 1725
Size: 422 692 611
Fragments arranged by size:
692 611 422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 568 832 1246 1267 1316 1582 1725
Size: 285 283 264 414 21 49 266 143
Fragments arranged by size:
414 285 283 266 264 143 49 21
Bpu10I CC'TnA_GC
Cuts at: 0 509 1725
Size: 509 1216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 514 1360 1725
Size: 210 304 846 365
Fragments arranged by size:
846 365 304 210
BsaI GGTCTCn'nnnn_
Cuts at: 0 997 1535 1725
Size: 997 538 190
Fragments arranged by size:
997 538 190
BsaHI Gr'CG_yC
Cuts at: 0 467 1725
Size: 467 1258
BsaWI w'CCGG_w
Cuts at: 0 132 1725
Size: 132 1593
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 857 887 1725
Size: 857 30 838
Fragments arranged by size:
857 838 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 1414 1417 1594 1597 1600 1725
Size: 94 1320 3 177 3 3 125
Fragments arranged by size:
1320 177 125 94 3 3 3
BseYI C'CCAG_C
Cuts at: 0 264 475 674 877 966 1516 1725
Size: 264 211 199 203 89 550 209
Fragments arranged by size:
550 264 211 209 203 199 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 556 1463 1492 1725
Size: 419 137 907 29 233
Fragments arranged by size:
907 419 233 137 29
BsiEI CG_ry'CG
Cuts at: 0 14 95 1725
Size: 14 81 1630
Fragments arranged by size:
1630 81 14
BsiHKAI G_wGCw'C
Cuts at: 0 200 530 1725
Size: 200 330 1195
Fragments arranged by size:
1195 330 200
BsmI GAATG_Cn'
Cuts at: 0 1461 1725
Size: 1461 264
BsmBI CGTCTCn'nnnn_
Cuts at: 0 474 1725
Size: 474 1251
Bsp1286I G_dGCh'C
Cuts at: 0 55 200 301 369 518 530 626 665
Size: 55 145 101 68 149 12 96 39
Cuts at: 665 675 1111 1159 1620 1725
Size: 10 436 48 461 105
Fragments arranged by size:
461 436 149 145 105 101 96 68
55 48 39 12 10
BspEI T'CCGG_A
Cuts at: 0 132 1725
Size: 132 1593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 447 578 582 1455 1514 1725
Size: 447 131 4 873 59 211
Fragments arranged by size:
873 447 211 131 59 4
BsrFI r'CCGG_y
Cuts at: 0 90 584 709 1232 1725
Size: 90 494 125 523 493
Fragments arranged by size:
523 494 493 125 90
BsrGI T'GTAC_A
Cuts at: 0 914 1725
Size: 914 811
BssHII G'CGCG_C
Cuts at: 0 580 1725
Size: 580 1145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 360 962 1725
Size: 360 602 763
Fragments arranged by size:
763 602 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 459 1224 1309 1725
Size: 459 765 85 416
Fragments arranged by size:
765 459 416 85
BstYI r'GATC_y
Cuts at: 0 995 1304 1725
Size: 995 309 421
Fragments arranged by size:
995 421 309
Bsu36I CC'TnA_GG
Cuts at: 0 692 1725
Size: 692 1033
BtgI C'CryG_G
Cuts at: 0 413 740 1308 1725
Size: 413 327 568 417
Fragments arranged by size:
568 417 413 327
BtsI GCAGTG_nn'
Cuts at: 0 241 679 729 1627 1725
Size: 241 438 50 898 98
Fragments arranged by size:
898 438 241 98 50
EaeI y'GGCC_r
Cuts at: 0 11 92 932 1349 1725
Size: 11 81 840 417 376
Fragments arranged by size:
840 417 376 81 11
EagI C'GGCC_G
Cuts at: 0 11 92 1725
Size: 11 81 1633
Fragments arranged by size:
1633 81 11
EarI CTCTTCn'nnn_
Cuts at: 0 1662 1709 1725
Size: 1662 47 16
Fragments arranged by size:
1662 47 16
EcoICRI GAG'CTC
Cuts at: 0 198 1725
Size: 198 1527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 568 832 1186 1246 1267 1316 1582
Size: 285 283 264 354 60 21 49 266
Cuts at: 1582 1643 1689 1725
Size: 61 46 36
Fragments arranged by size:
354 285 283 266 264 61 60 49
46 36 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 574 893 1024 1249 1725
Size: 574 319 131 225 476
Fragments arranged by size:
574 476 319 225 131
EcoO109I rG'GnC_Cy
Cuts at: 0 51 52 305 642 661 1017 1444 1533
Size: 51 1 253 337 19 356 427 89
Cuts at: 1533 1725
Size: 192
Fragments arranged by size:
427 356 337 253 192 89 51 19
1
EcoRI G'AATT_C
Cuts at: 0 1277 1725
Size: 1277 448
FseI GG_CCGG'CC
Cuts at: 0 94 1725
Size: 94 1631
HaeII r_GCGC'y
Cuts at: 0 748 1215 1725
Size: 748 467 510
Fragments arranged by size:
748 510 467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 857 889 1725
Size: 857 32 836
Fragments arranged by size:
857 836 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1725
Size: 56 1669
MscI TGG'CCA
Cuts at: 0 934 1351 1725
Size: 934 417 374
Fragments arranged by size:
934 417 374
MslI CAynn'nnrTG
Cuts at: 0 457 1573 1725
Size: 457 1116 152
Fragments arranged by size:
1116 457 152
MspA1I CmG'CkG
Cuts at: 0 108 415 970 1389 1725
Size: 108 307 555 419 336
Fragments arranged by size:
555 419 336 307 108
NaeI GCC'GGC
Cuts at: 0 92 586 1234 1725
Size: 92 494 648 491
Fragments arranged by size:
648 494 491 92
NcoI C'CATG_G
Cuts at: 0 740 1308 1725
Size: 740 568 417
Fragments arranged by size:
740 568 417
NgoMIV G'CCGG_C
Cuts at: 0 90 584 1232 1725
Size: 90 494 648 493
Fragments arranged by size:
648 494 493 90
PasI CC'CwG_GG
Cuts at: 0 518 1725
Size: 518 1207
PfoI T'CCnGG_A
Cuts at: 0 139 301 1725
Size: 139 162 1424
Fragments arranged by size:
1424 162 139
PpuMI rG'GwC_Cy
Cuts at: 0 305 1444 1533 1725
Size: 305 1139 89 192
Fragments arranged by size:
1139 305 192 89
PspOMI G'GGCC_C
Cuts at: 0 51 365 622 661 1725
Size: 51 314 257 39 1064
Fragments arranged by size:
1064 314 257 51 39
PspXI vC'TCGA_Gb
Cuts at: 0 56 1725
Size: 56 1669
PstI C_TGCA'G
Cuts at: 0 248 294 575 872 1199 1271 1298 1725
Size: 248 46 281 297 327 72 27 427
Fragments arranged by size:
427 327 297 281 248 72 46 27
PvuII CAG'CTG
Cuts at: 0 108 970 1389 1725
Size: 108 862 419 336
Fragments arranged by size:
862 419 336 108
SacI G_AGCT'C
Cuts at: 0 200 1725
Size: 200 1525
SacII CC_GC'GG
Cuts at: 0 416 1725
Size: 416 1309
SfcI C'TryA_G
Cuts at: 0 244 290 571 868 1195 1267 1294 1725
Size: 244 46 281 297 327 72 27 431
Fragments arranged by size:
431 327 297 281 244 72 46 27
SmlI C'TyrA_G
Cuts at: 0 56 225 529 1375 1725
Size: 56 169 304 846 350
Fragments arranged by size:
846 350 304 169 56
StuI AGG'CCT
Cuts at: 0 1171 1725
Size: 1171 554
StyI C'CwwG_G
Cuts at: 0 740 1308 1346 1679 1725
Size: 740 568 38 333 46
Fragments arranged by size:
740 568 333 46 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1021 1725
Size: 1021 704
TatI w'GTAC_w
Cuts at: 0 914 1725
Size: 914 811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 738 1524 1725
Size: 738 786 201
Fragments arranged by size:
786 738 201
XhoI C'TCGA_G
Cuts at: 0 56 1725
Size: 56 1669
Enzymes that do cut:
AarI AclI AcuI AfeI AlfI AlwNI ApaI ApoI
AscI AvaI BanI BanII BbsI BcgI BglI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrFI BsrGI BssHII BstAPI
BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI
PfoI PpuMI PspOMI PspXI PstI PvuII SacI SacII
SfcI SmlI StuI StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AflII AflIII AgeI AhdI AleI
AloI ApaLI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BclI BfrBI BmgBI BmtI BplI BsaAI
BsaBI BsiWI BspHI BsrBI BsrDI BssSI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EciI
EcoRV FalI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XmaI XmnI ZraI