Restriction map for
pF1KB3129
(Linear) (Six-base) MAPSORT of: KIBB3129.seq Check: 263 from: 1 to: 717
KIBB3129.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 560 717
Size: 560 157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 670 717
Size: 396 274 47
Fragments arranged by size:
396 274 47
AleI CACnn'nnGTG
Cuts at: 0 397 717
Size: 397 320
AvaI C'yCGr_G
Cuts at: 0 117 157 185 717
Size: 117 40 28 532
Fragments arranged by size:
532 117 40 28
BamHI G'GATC_C
Cuts at: 0 563 717
Size: 563 154
BanI G'GyrC_C
Cuts at: 0 106 235 264 548 697 717
Size: 106 129 29 284 149 20
Fragments arranged by size:
284 149 129 106 29 20
BanII G_rGCy'C
Cuts at: 0 410 717
Size: 410 307
BbeI G_GCGC'C
Cuts at: 0 110 239 717
Size: 110 129 478
Fragments arranged by size:
478 129 110
BbsI GAAGACnn'nnnn_
Cuts at: 0 658 717
Size: 658 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 107 717
Size: 73 34 610
Fragments arranged by size:
610 73 34
BglI GCCn_nnn'nGGC
Cuts at: 0 299 717
Size: 299 418
BlpI GC'TnA_GC
Cuts at: 0 256 717
Size: 256 461
Bme1580I G_kGCm'C
Cuts at: 0 164 717
Size: 164 553
BmrI ACTGGGnnnn_n'
Cuts at: 0 438 717
Size: 438 279
BsaHI Gr'CG_yC
Cuts at: 0 107 236 364 717
Size: 107 129 128 353
Fragments arranged by size:
353 129 128 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 717
Size: 295 30 392
Fragments arranged by size:
392 295 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 138 418 717
Size: 138 280 299
Fragments arranged by size:
299 280 138
BseYI C'CCAG_C
Cuts at: 0 10 152 581 610 717
Size: 10 142 429 29 107
Fragments arranged by size:
429 142 107 29 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 538 717
Size: 340 198 179
Fragments arranged by size:
340 198 179
BsiEI CG_ry'CG
Cuts at: 0 197 717
Size: 197 520
BsiHKAI G_wGCw'C
Cuts at: 0 410 717
Size: 410 307
Bsp1286I G_dGCh'C
Cuts at: 0 164 410 717
Size: 164 246 307
Fragments arranged by size:
307 246 164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 560 717
Size: 560 157
BsrFI r'CCGG_y
Cuts at: 0 298 545 717
Size: 298 247 172
Fragments arranged by size:
298 247 172
BstXI CCAn_nnnn'nTGG
Cuts at: 0 399 717
Size: 399 318
BstYI r'GATC_y
Cuts at: 0 563 717
Size: 563 154
BtgI C'CryG_G
Cuts at: 0 242 392 717
Size: 242 150 325
Fragments arranged by size:
325 242 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 501 717
Size: 501 216
EarI CTCTTCn'nnn_
Cuts at: 0 415 717
Size: 415 302
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 280 717
Size: 280 437
EcoICRI GAG'CTC
Cuts at: 0 408 717
Size: 408 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 670 717
Size: 396 274 47
Fragments arranged by size:
396 274 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 292 717
Size: 292 425
EcoO109I rG'GnC_Cy
Cuts at: 0 189 203 247 717
Size: 189 14 44 470
Fragments arranged by size:
470 189 44 14
HaeII r_GCGC'y
Cuts at: 0 110 239 717
Size: 110 129 478
Fragments arranged by size:
478 129 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 671 703 717
Size: 295 32 344 32 14
Fragments arranged by size:
344 295 32 32 14
HincII GTy'rAC
Cuts at: 0 486 717
Size: 486 231
KasI G'GCGC_C
Cuts at: 0 106 235 717
Size: 106 129 482
Fragments arranged by size:
482 129 106
MslI CAynn'nnrTG
Cuts at: 0 397 472 717
Size: 397 75 245
Fragments arranged by size:
397 245 75
MspA1I CmG'CkG
Cuts at: 0 213 244 354 717
Size: 213 31 110 363
Fragments arranged by size:
363 213 110 31
NaeI GCC'GGC
Cuts at: 0 300 717
Size: 300 417
NarI GG'CG_CC
Cuts at: 0 107 236 717
Size: 107 129 481
Fragments arranged by size:
481 129 107
NgoMIV G'CCGG_C
Cuts at: 0 298 717
Size: 298 419
NspI r_CATG'y
Cuts at: 0 618 717
Size: 618 99
PasI CC'CwG_GG
Cuts at: 0 693 717
Size: 693 24
PfoI T'CCnGG_A
Cuts at: 0 566 717
Size: 566 151
PpuMI rG'GwC_Cy
Cuts at: 0 189 203 717
Size: 189 14 514
Fragments arranged by size:
514 189 14
PsiI TTA'TAA
Cuts at: 0 62 717
Size: 62 655
PstI C_TGCA'G
Cuts at: 0 218 717
Size: 218 499
PvuII CAG'CTG
Cuts at: 0 354 717
Size: 354 363
SacI G_AGCT'C
Cuts at: 0 410 717
Size: 410 307
SacII CC_GC'GG
Cuts at: 0 245 717
Size: 245 472
SanDI GG'GwC_CC
Cuts at: 0 189 717
Size: 189 528
SapI GCTCTTCn'nnn_
Cuts at: 0 415 717
Size: 415 302
SfcI C'TryA_G
Cuts at: 0 214 717
Size: 214 503
SfoI GGC'GCC
Cuts at: 0 108 237 717
Size: 108 129 480
Fragments arranged by size:
480 129 108
SmaI CCC'GGG
Cuts at: 0 119 159 187 717
Size: 119 40 28 530
Fragments arranged by size:
530 119 40 28
SphI G_CATG'C
Cuts at: 0 618 717
Size: 618 99
SrfI GCCC'GGGC
Cuts at: 0 159 717
Size: 159 558
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 685 717
Size: 685 32
TatI w'GTAC_w
Cuts at: 0 50 717
Size: 50 667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 156 188 260 292 717
Size: 156 32 72 32 425
Fragments arranged by size:
425 156 72 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 193 717
Size: 193 524
XmaI C'CCGG_G
Cuts at: 0 117 157 185 717
Size: 117 40 28 532
Fragments arranged by size:
532 117 40 28
Enzymes that do cut:
AarI AcuI AleI AvaI BamHI BanI BanII BbeI
BbsI BcgI BglI BlpI Bme1580I BmrI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI
BstXI BstYI BtgI BtgZI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PfoI PpuMI PsiI
PstI PvuII SacI SacII SanDI SapI SfcI SfoI
SmaI SphI SrfI TaqII TatI TstI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI
BglII BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SspI StuI StyI SwaI XbaI
XcmI XhoI XmnI ZraI