Restriction map for
pF1KB3115
(Linear) (Six-base) MAPSORT of: KIBB3115.seq Check: 6006 from: 1 to: 1269
KIBB3115.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1162 1269
Size: 604 558 107
Fragments arranged by size:
604 558 107
AflII C'TTAA_G
Cuts at: 0 370 1269
Size: 370 899
AlwNI CAG_nnn'CTG
Cuts at: 0 351 1269
Size: 351 918
AvaI C'yCGr_G
Cuts at: 0 136 1269
Size: 136 1133
BanII G_rGCy'C
Cuts at: 0 144 603 1228 1269
Size: 144 459 625 41
Fragments arranged by size:
625 459 144 41
BclI T'GATC_A
Cuts at: 0 80 1128 1269
Size: 80 1048 141
Fragments arranged by size:
1048 141 80
BfrBI ATG'CAT
Cuts at: 0 775 1269
Size: 775 494
BmgBI CAC'GTC
Cuts at: 0 489 1269
Size: 489 780
BmrI ACTGGGnnnn_n'
Cuts at: 0 200 587 1269
Size: 200 387 682
Fragments arranged by size:
682 387 200
BmtI G_CTAG'C
Cuts at: 0 454 1269
Size: 454 815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1269
Size: 34 1235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 1269
Size: 35 1234
BsaBI GATnn'nnATC
Cuts at: 0 502 1269
Size: 502 767
BsaHI Gr'CG_yC
Cuts at: 0 4 170 1269
Size: 4 166 1099
Fragments arranged by size:
1099 166 4
BseYI C'CCAG_C
Cuts at: 0 301 1107 1269
Size: 301 806 162
Fragments arranged by size:
806 301 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1000 1269
Size: 997 3 269
Fragments arranged by size:
997 269 3
BsiHKAI G_wGCw'C
Cuts at: 0 137 275 603 1269
Size: 137 138 328 666
Fragments arranged by size:
666 328 138 137
Bsp1286I G_dGCh'C
Cuts at: 0 137 144 275 603 1228 1269
Size: 137 7 131 328 625 41
Fragments arranged by size:
625 328 137 131 41 7
BsrBI CCG'CTC
Cuts at: 0 1164 1269
Size: 1164 105
BsrDI GCAATG_nn'
Cuts at: 0 777 895 1269
Size: 777 118 374
Fragments arranged by size:
777 374 118
BsrGI T'GTAC_A
Cuts at: 0 150 1209 1269
Size: 150 1059 60
Fragments arranged by size:
1059 150 60
BssSI C'ACGA_G
Cuts at: 0 781 1269
Size: 781 488
BstBI TT'CG_AA
Cuts at: 0 221 1269
Size: 221 1048
Bsu36I CC'TnA_GG
Cuts at: 0 992 1269
Size: 992 277
BtgI C'CryG_G
Cuts at: 0 638 1175 1269
Size: 638 537 94
Fragments arranged by size:
638 537 94
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 72 107 1269
Size: 72 35 1162
Fragments arranged by size:
1162 72 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1069 1269
Size: 1069 200
EarI CTCTTCn'nnn_
Cuts at: 0 253 1269
Size: 253 1016
EcoICRI GAG'CTC
Cuts at: 0 601 1269
Size: 601 668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 604 1162 1269
Size: 34 570 558 107
Fragments arranged by size:
570 558 107 34
EcoRV GAT'ATC
Cuts at: 0 504 1269
Size: 504 765
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 909 1269
Size: 909 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 554 586 1269
Size: 554 32 683
Fragments arranged by size:
683 554 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 641 1269
Size: 60 581 628
Fragments arranged by size:
628 581 60
MspA1I CmG'CkG
Cuts at: 0 411 1269
Size: 411 858
NcoI C'CATG_G
Cuts at: 0 638 1175 1269
Size: 638 537 94
Fragments arranged by size:
638 537 94
NheI G'CTAG_C
Cuts at: 0 450 1269
Size: 450 819
NruI TCG'CGA
Cuts at: 0 863 1269
Size: 863 406
NsiI A_TGCA'T
Cuts at: 0 777 1269
Size: 777 492
NspI r_CATG'y
Cuts at: 0 427 1269
Size: 427 842
PsiI TTA'TAA
Cuts at: 0 908 1269
Size: 908 361
PvuII CAG'CTG
Cuts at: 0 411 1269
Size: 411 858
SacI G_AGCT'C
Cuts at: 0 603 1269
Size: 603 666
SapI GCTCTTCn'nnn_
Cuts at: 0 253 1269
Size: 253 1016
SexAI A'CCwGG_T
Cuts at: 0 830 1269
Size: 830 439
SmlI C'TyrA_G
Cuts at: 0 50 370 1269
Size: 50 320 899
Fragments arranged by size:
899 320 50
SspI AAT'ATT
Cuts at: 0 918 1269
Size: 918 351
StyI C'CwwG_G
Cuts at: 0 164 638 1175 1269
Size: 164 474 537 94
Fragments arranged by size:
537 474 164 94
TatI w'GTAC_w
Cuts at: 0 150 711 1209 1269
Size: 150 561 498 60
Fragments arranged by size:
561 498 150 60
Enzymes that do cut:
AcuI AflII AlwNI AvaI BanII BclI BfrBI BmgBI
BmrI BmtI BpmI BpuEI BsaBI BsaHI BseYI BsgI
BsiHKAI Bsp1286I BsrBI BsrDI BsrGI BssSI BstBI Bsu36I
BtgI CspCI DrdI EarI EcoICRI Eco57MI EcoRV FalI
Hin4I MmeI MspA1I NcoI NheI NruI NsiI NspI
PsiI PvuII SacI SapI SexAI SmlI SspI StyI
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BplI
Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrFI BssHII BstAPI
BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI DraI
DraIII EaeI EagI EciI EcoNI EcoO109I EcoRI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV
NotI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI