Restriction map for
pF1KB3113
(Linear) (Six-base) MAPSORT of: KIBB3113.seq Check: 5343 from: 1 to: 969
KIBB3113.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 864 871 969
Size: 500 364 7 98
Fragments arranged by size:
500 364 98 7
AflII C'TTAA_G
Cuts at: 0 681 969
Size: 681 288
AflIII A'CryG_T
Cuts at: 0 240 969
Size: 240 729
AleI CACnn'nnGTG
Cuts at: 0 901 969
Size: 901 68
AlwNI CAG_nnn'CTG
Cuts at: 0 537 969
Size: 537 432
ApaLI G'TGCA_C
Cuts at: 0 444 969
Size: 444 525
ApoI r'AATT_y
Cuts at: 0 633 745 969
Size: 633 112 224
Fragments arranged by size:
633 224 112
BamHI G'GATC_C
Cuts at: 0 153 420 969
Size: 153 267 549
Fragments arranged by size:
549 267 153
BanII G_rGCy'C
Cuts at: 0 895 969
Size: 895 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 877 969
Size: 877 92
BclI T'GATC_A
Cuts at: 0 528 969
Size: 528 441
BglII A'GATC_T
Cuts at: 0 935 969
Size: 935 34
Bme1580I G_kGCm'C
Cuts at: 0 448 969
Size: 448 521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 914 969
Size: 914 55
Bpu10I CC'TnA_GC
Cuts at: 0 172 332 969
Size: 172 160 637
Fragments arranged by size:
637 172 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 624 969
Size: 624 345
BsaI GGTCTCn'nnnn_
Cuts at: 0 494 969
Size: 494 475
BsaHI Gr'CG_yC
Cuts at: 0 40 48 969
Size: 40 8 921
Fragments arranged by size:
921 40 8
BseYI C'CCAG_C
Cuts at: 0 166 674 969
Size: 166 508 295
Fragments arranged by size:
508 295 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 969
Size: 569 400
BsiHKAI G_wGCw'C
Cuts at: 0 448 969
Size: 448 521
Bsp1286I G_dGCh'C
Cuts at: 0 448 895 969
Size: 448 447 74
Fragments arranged by size:
448 447 74
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 969
Size: 89 880
BsrBI CCG'CTC
Cuts at: 0 22 969
Size: 22 947
BstEII G'GTnAC_C
Cuts at: 0 642 969
Size: 642 327
BstXI CCAn_nnnn'nTGG
Cuts at: 0 502 903 969
Size: 502 401 66
Fragments arranged by size:
502 401 66
BstYI r'GATC_y
Cuts at: 0 153 420 935 969
Size: 153 267 515 34
Fragments arranged by size:
515 267 153 34
Bsu36I CC'TnA_GG
Cuts at: 0 522 969
Size: 522 447
BtgI C'CryG_G
Cuts at: 0 51 969
Size: 51 918
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 108 278 969
Size: 108 170 691
Fragments arranged by size:
691 170 108
BtsI GCAGTG_nn'
Cuts at: 0 287 307 969
Size: 287 20 662
Fragments arranged by size:
662 287 20
EarI CTCTTCn'nnn_
Cuts at: 0 836 969
Size: 836 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 864 871 969
Size: 500 364 7 98
Fragments arranged by size:
500 364 98 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 557 589 969
Size: 557 32 380
Fragments arranged by size:
557 380 32
HincII GTy'rAC
Cuts at: 0 657 969
Size: 657 312
HindIII A'AGCT_T
Cuts at: 0 186 969
Size: 186 783
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 448 969
Size: 411 37 521
Fragments arranged by size:
521 411 37
MslI CAynn'nnrTG
Cuts at: 0 725 901 969
Size: 725 176 68
Fragments arranged by size:
725 176 68
NspI r_CATG'y
Cuts at: 0 244 969
Size: 244 725
PciI A'CATG_T
Cuts at: 0 240 969
Size: 240 729
PflMI CCAn_nnn'nTGG
Cuts at: 0 430 969
Size: 430 539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 969
Size: 230 32 707
Fragments arranged by size:
707 230 32
PshAI GACnn'nnGTC
Cuts at: 0 881 969
Size: 881 88
PstI C_TGCA'G
Cuts at: 0 278 303 969
Size: 278 25 666
Fragments arranged by size:
666 278 25
SfcI C'TryA_G
Cuts at: 0 255 274 299 660 969
Size: 255 19 25 361 309
Fragments arranged by size:
361 309 255 25 19
SmlI C'TyrA_G
Cuts at: 0 603 681 969
Size: 603 78 288
Fragments arranged by size:
603 288 78
SspI AAT'ATT
Cuts at: 0 149 969
Size: 149 820
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApaLI ApoI BamHI
BanII BbsI BclI BglII Bme1580I BplI Bpu10I BpuEI
BsaI BsaHI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EarI
Eco57MI Hin4I HincII HindIII MmeI MslI NspI PciI
PflMI PpiI PshAI PstI SfcI SmlI SspI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BfrBI BglI
BlpI BmgBI BmrI BmtI BpmI BsaAI BsaBI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PpuMI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI