Restriction map for
pF1KB3110
(Linear) (Six-base) MAPSORT of: KIBB3110.seq Check: 3939 from: 1 to: 891
KIBB3110.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
Acc65I G'GTAC_C
Cuts at: 0 107 619 891
Size: 107 512 272
Fragments arranged by size:
512 272 107
AflIII A'CryG_T
Cuts at: 0 739 891
Size: 739 152
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 505 537 891
Size: 505 32 354
Fragments arranged by size:
505 354 32
AlwNI CAG_nnn'CTG
Cuts at: 0 84 93 891
Size: 84 9 798
Fragments arranged by size:
798 84 9
ApaI G_GGCC'C
Cuts at: 0 66 891
Size: 66 825
AvaI C'yCGr_G
Cuts at: 0 162 229 548 580 587 853 891
Size: 162 67 319 32 7 266 38
Fragments arranged by size:
319 266 162 67 38 32 7
BanI G'GyrC_C
Cuts at: 0 107 166 571 619 891
Size: 107 59 405 48 272
Fragments arranged by size:
405 272 107 59 48
BanII G_rGCy'C
Cuts at: 0 66 581 587 676 891
Size: 66 515 6 89 215
Fragments arranged by size:
515 215 89 66 6
BbeI G_GCGC'C
Cuts at: 0 170 891
Size: 170 721
BbsI GAAGACnn'nnnn_
Cuts at: 0 889 891
Size: 889 2
BglI GCCn_nnn'nGGC
Cuts at: 0 266 891
Size: 266 625
BlpI GC'TnA_GC
Cuts at: 0 424 467 891
Size: 424 43 424
Fragments arranged by size:
424 424 43
Bme1580I G_kGCm'C
Cuts at: 0 66 809 891
Size: 66 743 82
Fragments arranged by size:
743 82 66
BmgBI CAC'GTC
Cuts at: 0 288 804 891
Size: 288 516 87
Fragments arranged by size:
516 288 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 891
Size: 685 206
Bpu10I CC'TnA_GC
Cuts at: 0 649 891
Size: 649 242
BsaAI yAC'GTr
Cuts at: 0 740 768 891
Size: 740 28 123
Fragments arranged by size:
740 123 28
BsaHI Gr'CG_yC
Cuts at: 0 98 167 302 752 891
Size: 98 69 135 450 139
Fragments arranged by size:
450 139 135 98 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 805 835 891
Size: 805 30 56
Fragments arranged by size:
805 56 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 694 891
Size: 163 531 197
Fragments arranged by size:
531 197 163
BseYI C'CCAG_C
Cuts at: 0 73 120 891
Size: 73 47 771
Fragments arranged by size:
771 73 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 891
Size: 213 678
BsiEI CG_ry'CG
Cuts at: 0 201 891
Size: 201 690
BsmBI CGTCTCn'nnnn_
Cuts at: 0 584 891
Size: 584 307
Bsp1286I G_dGCh'C
Cuts at: 0 66 581 587 676 809 891
Size: 66 515 6 89 133 82
Fragments arranged by size:
515 133 89 82 66 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 453 551 891
Size: 119 334 98 340
Fragments arranged by size:
340 334 119 98
BsrBI CCG'CTC
Cuts at: 0 423 891
Size: 423 468
BsrDI GCAATG_nn'
Cuts at: 0 46 891
Size: 46 845
BssHII G'CGCG_C
Cuts at: 0 23 69 843 891
Size: 23 46 774 48
Fragments arranged by size:
774 48 46 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 891
Size: 84 807
BstYI r'GATC_y
Cuts at: 0 683 891
Size: 683 208
Bsu36I CC'TnA_GG
Cuts at: 0 677 891
Size: 677 214
BtgI C'CryG_G
Cuts at: 0 567 891
Size: 567 324
BtsI GCAGTG_nn'
Cuts at: 0 376 891
Size: 376 515
DraIII CAC_nnn'GTG
Cuts at: 0 291 372 891
Size: 291 81 519
Fragments arranged by size:
519 291 81
EaeI y'GGCC_r
Cuts at: 0 278 360 606 734 891
Size: 278 82 246 128 157
Fragments arranged by size:
278 246 157 128 82
EarI CTCTTCn'nnn_
Cuts at: 0 664 891
Size: 664 227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 315 385 676 891
Size: 64 251 70 291 215
Fragments arranged by size:
291 251 215 70 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 891
Size: 685 206
EcoNI CCTnn'n_nnAGG
Cuts at: 0 652 891
Size: 652 239
EcoO109I rG'GnC_Cy
Cuts at: 0 63 315 779 891
Size: 63 252 464 112
Fragments arranged by size:
464 252 112 63
HaeII r_GCGC'y
Cuts at: 0 58 156 170 531 891
Size: 58 98 14 361 360
Fragments arranged by size:
361 360 98 58 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 675 707 850 882 891
Size: 190 32 453 32 143 32 9
Fragments arranged by size:
453 190 143 32 32 32 9
KasI G'GCGC_C
Cuts at: 0 166 891
Size: 166 725
KpnI G_GTAC'C
Cuts at: 0 111 623 891
Size: 111 512 268
Fragments arranged by size:
512 268 111
MscI TGG'CCA
Cuts at: 0 608 736 891
Size: 608 128 155
Fragments arranged by size:
608 155 128
MspA1I CmG'CkG
Cuts at: 0 90 569 891
Size: 90 479 322
Fragments arranged by size:
479 322 90
NarI GG'CG_CC
Cuts at: 0 167 891
Size: 167 724
NspI r_CATG'y
Cuts at: 0 225 843 891
Size: 225 618 48
Fragments arranged by size:
618 225 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 540 891
Size: 540 351
PfoI T'CCnGG_A
Cuts at: 0 698 891
Size: 698 193
PmlI CAC'GTG
Cuts at: 0 740 768 891
Size: 740 28 123
Fragments arranged by size:
740 123 28
PspOMI G'GGCC_C
Cuts at: 0 62 891
Size: 62 829
PstI C_TGCA'G
Cuts at: 0 89 891
Size: 89 802
PvuII CAG'CTG
Cuts at: 0 90 891
Size: 90 801
SacII CC_GC'GG
Cuts at: 0 570 891
Size: 570 321
SapI GCTCTTCn'nnn_
Cuts at: 0 664 891
Size: 664 227
SfcI C'TryA_G
Cuts at: 0 85 891
Size: 85 806
SfoI GGC'GCC
Cuts at: 0 168 891
Size: 168 723
SmaI CCC'GGG
Cuts at: 0 164 231 891
Size: 164 67 660
Fragments arranged by size:
660 164 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 298 816 891
Size: 33 265 518 75
Fragments arranged by size:
518 265 75 33
XmaI C'CCGG_G
Cuts at: 0 162 229 891
Size: 162 67 662
Fragments arranged by size:
662 162 67
Enzymes that do cut:
Acc65I AflIII AloI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BmgBI BpmI Bpu10I
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI
Bsp1286I BspMI BsrBI BsrDI BssHII BstAPI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI KpnI MscI MspA1I NarI
NspI PflMI PfoI PmlI PspOMI PstI PvuII SacII
SapI SfcI SfoI SmaI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BmrI BmtI BplI BpuEI BsaI BsaBI BsaWI
BsiHKAI BsiWI BsmI BspEI BspHI BsrFI BsrGI BssSI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI MmeI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI