Restriction map for
pF1KB3104
(Linear) (Six-base) MAPSORT of: KIBB3104.seq Check: 1238 from: 1 to: 2409
KIBB3104.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2023 2409
Size: 2023 386
AccI GT'mk_AC
Cuts at: 0 457 2409
Size: 457 1952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 523 1413 1425 1666 1921 2337 2409
Size: 207 316 890 12 241 255 416 72
Fragments arranged by size:
890 416 316 255 241 207 72 12
AfeI AGC'GCT
Cuts at: 0 24 1434 2409
Size: 24 1410 975
Fragments arranged by size:
1410 975 24
AflIII A'CryG_T
Cuts at: 0 612 882 1025 1554 2409
Size: 612 270 143 529 855
Fragments arranged by size:
855 612 529 270 143
AgeI A'CCGG_T
Cuts at: 0 2321 2409
Size: 2321 88
AleI CACnn'nnGTG
Cuts at: 0 726 967 2409
Size: 726 241 1442
Fragments arranged by size:
1442 726 241
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2147 2179 2409
Size: 2147 32 230
Fragments arranged by size:
2147 230 32
AlwNI CAG_nnn'CTG
Cuts at: 0 60 683 1644 2166 2316 2409
Size: 60 623 961 522 150 93
Fragments arranged by size:
961 623 522 150 93 60
ApaI G_GGCC'C
Cuts at: 0 559 2409
Size: 559 1850
ApaLI G'TGCA_C
Cuts at: 0 608 1504 2409
Size: 608 896 905
Fragments arranged by size:
905 896 608
ApoI r'AATT_y
Cuts at: 0 239 277 482 1189 2195 2409
Size: 239 38 205 707 1006 214
Fragments arranged by size:
1006 707 239 214 205 38
AvaI C'yCGr_G
Cuts at: 0 250 1707 2409
Size: 250 1457 702
Fragments arranged by size:
1457 702 250
BamHI G'GATC_C
Cuts at: 0 495 2357 2409
Size: 495 1862 52
Fragments arranged by size:
1862 495 52
BanI G'GyrC_C
Cuts at: 0 1702 2409
Size: 1702 707
BanII G_rGCy'C
Cuts at: 0 559 797 2409
Size: 559 238 1612
Fragments arranged by size:
1612 559 238
BbsI GAAGACnn'nnnn_
Cuts at: 0 236 715 2409
Size: 236 479 1694
Fragments arranged by size:
1694 479 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 732 766 975 1009 2409
Size: 732 34 209 34 1400
Fragments arranged by size:
1400 732 209 34 34
BclI T'GATC_A
Cuts at: 0 2168 2409
Size: 2168 241
BglI GCCn_nnn'nGGC
Cuts at: 0 563 2409
Size: 563 1846
BglII A'GATC_T
Cuts at: 0 216 1310 2409
Size: 216 1094 1099
Fragments arranged by size:
1099 1094 216
Bme1580I G_kGCm'C
Cuts at: 0 559 612 1508 2409
Size: 559 53 896 901
Fragments arranged by size:
901 896 559 53
BmgBI CAC'GTC
Cuts at: 0 1272 2409
Size: 1272 1137
BmrI ACTGGGnnnn_n'
Cuts at: 0 1275 2409
Size: 1275 1134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 92 2409
Size: 92 2317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 937 1882 2283 2409
Size: 383 554 945 401 126
Fragments arranged by size:
945 554 401 383 126
Bpu10I CC'TnA_GC
Cuts at: 0 475 1321 1963 2409
Size: 475 846 642 446
Fragments arranged by size:
846 642 475 446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1453 2409
Size: 1453 956
BsaI GGTCTCn'nnnn_
Cuts at: 0 645 737 2409
Size: 645 92 1672
Fragments arranged by size:
1672 645 92
BsaHI Gr'CG_yC
Cuts at: 0 1049 2409
Size: 1049 1360
BsaWI w'CCGG_w
Cuts at: 0 735 2321 2409
Size: 735 1586 88
Fragments arranged by size:
1586 735 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 796 826 2409
Size: 796 30 1583
Fragments arranged by size:
1583 796 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 712 1879 2409
Size: 712 1167 530
Fragments arranged by size:
1167 712 530
BseYI C'CCAG_C
Cuts at: 0 868 1217 1745 1851 1980 2409
Size: 868 349 528 106 129 429
Fragments arranged by size:
868 528 429 349 129 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1050 1404 1816 2409
Size: 61 989 354 412 593
Fragments arranged by size:
989 593 412 354 61
BsiEI CG_ry'CG
Cuts at: 0 1800 2409
Size: 1800 609
BsiHKAI G_wGCw'C
Cuts at: 0 612 797 1327 1508 1969 2409
Size: 612 185 530 181 461 440
Fragments arranged by size:
612 530 461 440 185 181
BsmI GAATG_Cn'
Cuts at: 0 2353 2409
Size: 2353 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1338 1744 2409
Size: 1338 406 665
Fragments arranged by size:
1338 665 406
Bsp1286I G_dGCh'C
Cuts at: 0 559 612 797 1327 1508 1969 2409
Size: 559 53 185 530 181 461 440
Fragments arranged by size:
559 530 461 440 185 181 53
BspEI T'CCGG_A
Cuts at: 0 735 2409
Size: 735 1674
BspHI T'CATG_A
Cuts at: 0 243 2409
Size: 243 2166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1343 1637 2023 2409
Size: 1343 294 386 386
Fragments arranged by size:
1343 386 386 294
BsrDI GCAATG_nn'
Cuts at: 0 534 2409
Size: 534 1875
BsrFI r'CCGG_y
Cuts at: 0 808 2321 2409
Size: 808 1513 88
Fragments arranged by size:
1513 808 88
BssSI C'ACGA_G
Cuts at: 0 744 1078 2409
Size: 744 334 1331
Fragments arranged by size:
1331 744 334
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 1371 2409
Size: 636 735 1038
Fragments arranged by size:
1038 735 636
BstYI r'GATC_y
Cuts at: 0 216 495 1280 1310 2357 2409
Size: 216 279 785 30 1047 52
Fragments arranged by size:
1047 785 279 216 52 30
BstZ17I GTA'TAC
Cuts at: 0 458 2409
Size: 458 1951
BtgI C'CryG_G
Cuts at: 0 940 965 1055 1770 1800 2091 2409
Size: 940 25 90 715 30 291 318
Fragments arranged by size:
940 715 318 291 90 30 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 702 2409
Size: 702 1707
BtsI GCAGTG_nn'
Cuts at: 0 109 1396 2180 2409
Size: 109 1287 784 229
Fragments arranged by size:
1287 784 229 109
DraIII CAC_nnn'GTG
Cuts at: 0 1839 2409
Size: 1839 570
EaeI y'GGCC_r
Cuts at: 0 1140 1797 1931 2409
Size: 1140 657 134 478
Fragments arranged by size:
1140 657 478 134
EagI C'GGCC_G
Cuts at: 0 1797 2409
Size: 1797 612
EarI CTCTTCn'nnn_
Cuts at: 0 226 689 2409
Size: 226 463 1720
Fragments arranged by size:
1720 463 226
EcoICRI GAG'CTC
Cuts at: 0 795 2409
Size: 795 1614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 383 523 937 1413 1425 1666 1882
Size: 207 176 140 414 476 12 241 216
Cuts at: 1882 1921 2283 2337 2409
Size: 39 362 54 72
Fragments arranged by size:
476 414 362 241 216 207 176 140
72 54 39 12
EcoO109I rG'GnC_Cy
Cuts at: 0 555 556 1332 1480 2409
Size: 555 1 776 148 929
Fragments arranged by size:
929 776 555 148 1
EcoRI G'AATT_C
Cuts at: 0 277 2409
Size: 277 2132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1170 1332 2409
Size: 1170 162 1077
Fragments arranged by size:
1170 1077 162
HaeII r_GCGC'y
Cuts at: 0 26 1436 2409
Size: 26 1410 973
Fragments arranged by size:
1410 973 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 455 487 1337 1369 2160 2192 2409
Size: 455 32 850 32 791 32 217
Fragments arranged by size:
850 791 455 217 32 32 32
HindIII A'AGCT_T
Cuts at: 0 535 2409
Size: 535 1874
MfeI C'AATT_G
Cuts at: 0 104 2409
Size: 104 2305
MslI CAynn'nnrTG
Cuts at: 0 634 726 964 967 1082 1204 2409
Size: 634 92 238 3 115 122 1205
Fragments arranged by size:
1205 634 238 122 115 92 3
MspA1I CmG'CkG
Cuts at: 0 172 573 1808 2409
Size: 172 401 1235 601
Fragments arranged by size:
1235 601 401 172
NcoI C'CATG_G
Cuts at: 0 965 2091 2409
Size: 965 1126 318
Fragments arranged by size:
1126 965 318
NspI r_CATG'y
Cuts at: 0 616 1558 2409
Size: 616 942 851
Fragments arranged by size:
942 851 616
PasI CC'CwG_GG
Cuts at: 0 1173 2409
Size: 1173 1236
PciI A'CATG_T
Cuts at: 0 612 1554 2409
Size: 612 942 855
Fragments arranged by size:
942 855 612
PflMI CCAn_nnn'nTGG
Cuts at: 0 915 1452 2409
Size: 915 537 957
Fragments arranged by size:
957 915 537
PfoI T'CCnGG_A
Cuts at: 0 2360 2409
Size: 2360 49
PpuMI rG'GwC_Cy
Cuts at: 0 1332 1480 2409
Size: 1332 148 929
Fragments arranged by size:
1332 929 148
PspOMI G'GGCC_C
Cuts at: 0 555 2409
Size: 555 1854
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1408 1440 2409
Size: 1408 32 969
Fragments arranged by size:
1408 969 32
PvuII CAG'CTG
Cuts at: 0 573 2409
Size: 573 1836
SacI G_AGCT'C
Cuts at: 0 797 2409
Size: 797 1612
ScaI AGT'ACT
Cuts at: 0 955 2409
Size: 955 1454
SexAI A'CCwGG_T
Cuts at: 0 1406 2409
Size: 1406 1003
SfcI C'TryA_G
Cuts at: 0 209 285 1356 2409
Size: 209 76 1071 1053
Fragments arranged by size:
1071 1053 209 76
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 563 2409
Size: 563 1846
SmaI CCC'GGG
Cuts at: 0 1709 2409
Size: 1709 700
SmlI C'TyrA_G
Cuts at: 0 1468 2409
Size: 1468 941
StuI AGG'CCT
Cuts at: 0 1096 2409
Size: 1096 1313
StyI C'CwwG_G
Cuts at: 0 11 848 965 989 1133 1815 2091 2123
Size: 11 837 117 24 144 682 276 32
Cuts at: 2123 2409
Size: 286
Fragments arranged by size:
837 682 286 276 144 117 32 24
11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 922 1592 1687 2409
Size: 141 781 670 95 722
Fragments arranged by size:
781 722 670 141 95
TatI w'GTAC_w
Cuts at: 0 953 1910 2409
Size: 953 957 499
Fragments arranged by size:
957 953 499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 912 2409
Size: 912 1497
XmaI C'CCGG_G
Cuts at: 0 1707 2409
Size: 1707 702
XmnI GAAnn'nnTTC
Cuts at: 0 2195 2409
Size: 2195 214
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BcgI BclI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BssSI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HindIII
MfeI MslI MspA1I NcoI NspI PasI PciI PflMI
PfoI PpuMI PspOMI PsrI PvuII SacI ScaI SexAI
SfcI SfiI SmaI SmlI StuI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AhdI AlfI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BciVI BfrBI BlpI
BmtI BsaAI BsaBI BsiWI BsrBI BsrGI BssHII BstAPI
BstBI BstEII Bsu36I ClaI CspCI DraI DrdI EciI
EcoNI EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
ZraI