Restriction map for
pF1KB3098
(Linear) (Six-base) MAPSORT of: KIBB3098.seq Check: 1001 from: 1 to: 1938
KIBB3098.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 883 1938
Size: 883 1055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 1658 1938
Size: 1352 306 280
Fragments arranged by size:
1352 306 280
AlwNI CAG_nnn'CTG
Cuts at: 0 72 95 1938
Size: 72 23 1843
Fragments arranged by size:
1843 72 23
ApaI G_GGCC'C
Cuts at: 0 218 1938
Size: 218 1720
ApoI r'AATT_y
Cuts at: 0 670 892 925 1621 1770 1938
Size: 670 222 33 696 149 168
Fragments arranged by size:
696 670 222 168 149 33
AscI GG'CGCG_CC
Cuts at: 0 466 1938
Size: 466 1472
AseI AT'TA_AT
Cuts at: 0 704 1938
Size: 704 1234
AvaI C'yCGr_G
Cuts at: 0 211 217 235 297 510 593 1938
Size: 211 6 18 62 213 83 1345
Fragments arranged by size:
1345 213 211 83 62 18 6
BanI G'GyrC_C
Cuts at: 0 289 514 1938
Size: 289 225 1424
Fragments arranged by size:
1424 289 225
BanII G_rGCy'C
Cuts at: 0 173 202 212 218 1009 1938
Size: 173 29 10 6 791 929
Fragments arranged by size:
929 791 173 29 10 6
BbeI G_GCGC'C
Cuts at: 0 293 1938
Size: 293 1645
BbsI GAAGACnn'nnnn_
Cuts at: 0 800 1674 1938
Size: 800 874 264
Fragments arranged by size:
874 800 264
BbvCI CC'TCA_GC
Cuts at: 0 1823 1938
Size: 1823 115
BciVI GTATCCnnnnn_n'
Cuts at: 0 639 1592 1938
Size: 639 953 346
Fragments arranged by size:
953 639 346
BclI T'GATC_A
Cuts at: 0 1107 1938
Size: 1107 831
BfrBI ATG'CAT
Cuts at: 0 968 1938
Size: 968 970
BglI GCCn_nnn'nGGC
Cuts at: 0 49 476 1938
Size: 49 427 1462
Fragments arranged by size:
1462 427 49
Bme1580I G_kGCm'C
Cuts at: 0 218 517 1938
Size: 218 299 1421
Fragments arranged by size:
1421 299 218
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 192 351 1938
Size: 192 159 1587
Fragments arranged by size:
1587 192 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 941 1395 1938
Size: 286 655 454 543
Fragments arranged by size:
655 543 454 286
Bpu10I CC'TnA_GC
Cuts at: 0 10 1823 1938
Size: 10 1813 115
Fragments arranged by size:
1813 115 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 1260 1777 1938
Size: 677 583 517 161
Fragments arranged by size:
677 583 517 161
BsaHI Gr'CG_yC
Cuts at: 0 290 1938
Size: 290 1648
BsaWI w'CCGG_w
Cuts at: 0 717 1847 1938
Size: 717 1130 91
Fragments arranged by size:
1130 717 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 262 292 1938
Size: 262 30 1646
Fragments arranged by size:
1646 262 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 118 130 133 136 139 283 351
Size: 115 3 12 3 3 3 144 68
Cuts at: 351 358 361 680 1441 1938
Size: 7 3 319 761 497
Fragments arranged by size:
761 497 319 144 115 68 12 7
3 3 3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1748 1938
Size: 39 1709 190
Fragments arranged by size:
1709 190 39
BsiEI CG_ry'CG
Cuts at: 0 502 1938
Size: 502 1436
BsiHKAI G_wGCw'C
Cuts at: 0 1836 1938
Size: 1836 102
BsmI GAATG_Cn'
Cuts at: 0 1065 1695 1758 1938
Size: 1065 630 63 180
Fragments arranged by size:
1065 630 180 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1334 1938
Size: 1334 604
Bsp1286I G_dGCh'C
Cuts at: 0 173 202 212 218 517 1009 1836 1938
Size: 173 29 10 6 299 492 827 102
Fragments arranged by size:
827 492 299 173 102 29 10 6
BspEI T'CCGG_A
Cuts at: 0 717 1938
Size: 717 1221
BspHI T'CATG_A
Cuts at: 0 897 1181 1938
Size: 897 284 757
Fragments arranged by size:
897 757 284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 846 1938
Size: 846 1092
BsrBI CCG'CTC
Cuts at: 0 230 491 1916 1938
Size: 230 261 1425 22
Fragments arranged by size:
1425 261 230 22
BsrDI GCAATG_nn'
Cuts at: 0 1685 1938
Size: 1685 253
BsrFI r'CCGG_y
Cuts at: 0 240 369 381 417 1938
Size: 240 129 12 36 1521
Fragments arranged by size:
1521 240 129 36 12
BssHII G'CGCG_C
Cuts at: 0 466 1938
Size: 466 1472
BssSI C'ACGA_G
Cuts at: 0 336 1938
Size: 336 1602
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1816 1938
Size: 1816 122
BstYI r'GATC_y
Cuts at: 0 1212 1406 1656 1938
Size: 1212 194 250 282
Fragments arranged by size:
1212 282 250 194
BstZ17I GTA'TAC
Cuts at: 0 884 1938
Size: 884 1054
Bsu36I CC'TnA_GG
Cuts at: 0 741 1938
Size: 741 1197
BtgI C'CryG_G
Cuts at: 0 203 451 496 1938
Size: 203 248 45 1442
Fragments arranged by size:
1442 248 203 45
BtsI GCAGTG_nn'
Cuts at: 0 1058 1938
Size: 1058 880
DraI TTT'AAA
Cuts at: 0 1194 1377 1938
Size: 1194 183 561
Fragments arranged by size:
1194 561 183
DrdI GACnn_nn'nnGTC
Cuts at: 0 752 1938
Size: 752 1186
EaeI y'GGCC_r
Cuts at: 0 135 499 1230 1853 1938
Size: 135 364 731 623 85
Fragments arranged by size:
731 623 364 135 85
EagI C'GGCC_G
Cuts at: 0 499 1938
Size: 499 1439
EarI CTCTTCn'nnn_
Cuts at: 0 107 556 643 946 1550 1843 1938
Size: 107 449 87 303 604 293 95
Fragments arranged by size:
604 449 303 293 107 95 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 340 1938
Size: 340 1598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 941 1352 1395 1658 1938
Size: 286 655 411 43 263 280
Fragments arranged by size:
655 411 286 280 263 43
EcoO109I rG'GnC_Cy
Cuts at: 0 273 1938
Size: 273 1665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 93 1938
Size: 93 1845
FseI GG_CCGG'CC
Cuts at: 0 244 1938
Size: 244 1694
HaeII r_GCGC'y
Cuts at: 0 225 293 1938
Size: 225 68 1645
Fragments arranged by size:
1645 225 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 1342 1374 1398 1430 1938
Size: 595 32 715 32 24 32 508
Fragments arranged by size:
715 595 508 32 32 32 24
KasI G'GCGC_C
Cuts at: 0 289 1938
Size: 289 1649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 1938
Size: 1385 553
MscI TGG'CCA
Cuts at: 0 1855 1938
Size: 1855 83
MslI CAynn'nnrTG
Cuts at: 0 1070 1938
Size: 1070 868
MspA1I CmG'CkG
Cuts at: 0 134 183 453 498 1822 1938
Size: 134 49 270 45 1324 116
Fragments arranged by size:
1324 270 134 116 49 45
NaeI GCC'GGC
Cuts at: 0 242 383 1938
Size: 242 141 1555
Fragments arranged by size:
1555 242 141
NarI GG'CG_CC
Cuts at: 0 290 1938
Size: 290 1648
NdeI CA'TA_TG
Cuts at: 0 1112 1938
Size: 1112 826
NgoMIV G'CCGG_C
Cuts at: 0 240 381 1938
Size: 240 141 1557
Fragments arranged by size:
1557 240 141
NsiI A_TGCA'T
Cuts at: 0 970 1938
Size: 970 968
NspI r_CATG'y
Cuts at: 0 1399 1938
Size: 1399 539
PpuMI rG'GwC_Cy
Cuts at: 0 273 1938
Size: 273 1665
PspOMI G'GGCC_C
Cuts at: 0 214 1938
Size: 214 1724
PstI C_TGCA'G
Cuts at: 0 91 1821 1938
Size: 91 1730 117
Fragments arranged by size:
1730 117 91
PvuII CAG'CTG
Cuts at: 0 1822 1938
Size: 1822 116
SacII CC_GC'GG
Cuts at: 0 454 499 1938
Size: 454 45 1439
Fragments arranged by size:
1439 454 45
SfcI C'TryA_G
Cuts at: 0 87 1173 1817 1938
Size: 87 1086 644 121
Fragments arranged by size:
1086 644 121 87
SfoI GGC'GCC
Cuts at: 0 291 1938
Size: 291 1647
SgrAI Cr'CCGG_yG
Cuts at: 0 381 417 1938
Size: 381 36 1521
Fragments arranged by size:
1521 381 36
SmaI CCC'GGG
Cuts at: 0 213 237 299 512 1938
Size: 213 24 62 213 1426
Fragments arranged by size:
1426 213 213 62 24
SmlI C'TyrA_G
Cuts at: 0 692 1239 1792 1938
Size: 692 547 553 146
Fragments arranged by size:
692 553 547 146
SrfI GCCC'GGGC
Cuts at: 0 213 237 299 1938
Size: 213 24 62 1639
Fragments arranged by size:
1639 213 62 24
SspI AAT'ATT
Cuts at: 0 702 1132 1293 1938
Size: 702 430 161 645
Fragments arranged by size:
702 645 430 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1885 1938
Size: 1885 53
Tth111I GACn'n_nGTC
Cuts at: 0 749 1938
Size: 749 1189
XbaI T'CTAG_A
Cuts at: 0 1717 1938
Size: 1717 221
XmaI C'CCGG_G
Cuts at: 0 211 235 297 510 1938
Size: 211 24 62 213 1428
Fragments arranged by size:
1428 213 211 62 24
XmnI GAAnn'nnTTC
Cuts at: 0 603 1544 1671 1938
Size: 603 941 127 267
Fragments arranged by size:
941 603 267 127
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI AscI AseI AvaI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI Bme1580I BplI BpmI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstYI BstZ17I Bsu36I BtgI BtsI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I FalI FseI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PpuMI PspOMI PstI
PvuII SacII SfcI SfoI SgrAI SmaI SmlI SrfI
SspI TaqII Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AsiSI AvrII BaeI
BamHI BcgI BglII BlpI BmgBI BmrI BmtI BsaI
BsaAI BsaBI BseYI BsiWI BsrGI BstBI BstEII BstXI
BtgZI ClaI CspCI DraIII EcoICRI EcoNI EcoRI EcoRV
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NcoI NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SnaBI SpeI SphI StuI StyI
SwaI TatI TstI XcmI XhoI ZraI