Restriction map for
pF1KB3093
(Linear) (Six-base) MAPSORT of: KIBB3093.seq Check: 1458 from: 1 to: 2235
KIBB3093.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 103 2235
Size: 103 2132
AatII G_ACGT'C
Cuts at: 0 1214 2235
Size: 1214 1021
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1198 2235
Size: 1198 1037
AhdI GACnn_n'nnGTC
Cuts at: 0 992 2235
Size: 992 1243
AleI CACnn'nnGTG
Cuts at: 0 97 313 2235
Size: 97 216 1922
Fragments arranged by size:
1922 216 97
AlwNI CAG_nnn'CTG
Cuts at: 0 345 882 1068 1776 2235
Size: 345 537 186 708 459
Fragments arranged by size:
708 537 459 345 186
ApaI G_GGCC'C
Cuts at: 0 534 935 2235
Size: 534 401 1300
Fragments arranged by size:
1300 534 401
AvaI C'yCGr_G
Cuts at: 0 69 81 433 1712 2235
Size: 69 12 352 1279 523
Fragments arranged by size:
1279 523 352 69 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 57 90 2235
Size: 57 33 2145
Fragments arranged by size:
2145 57 33
BanI G'GyrC_C
Cuts at: 0 85 149 192 775 819 2235
Size: 85 64 43 583 44 1416
Fragments arranged by size:
1416 583 85 64 44 43
BanII G_rGCy'C
Cuts at: 0 440 534 544 935 2003 2075 2235
Size: 440 94 10 391 1068 72 160
Fragments arranged by size:
1068 440 391 160 94 72 10
BbeI G_GCGC'C
Cuts at: 0 89 153 779 2235
Size: 89 64 626 1456
Fragments arranged by size:
1456 626 89 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1903 1937 2235
Size: 1903 34 298
Fragments arranged by size:
1903 298 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 85 2235
Size: 85 2150
BclI T'GATC_A
Cuts at: 0 911 1511 2235
Size: 911 600 724
Fragments arranged by size:
911 724 600
BglI GCCn_nnn'nGGC
Cuts at: 0 415 784 1411 2041 2235
Size: 415 369 627 630 194
Fragments arranged by size:
630 627 415 369 194
BglII A'GATC_T
Cuts at: 0 1736 2235
Size: 1736 499
BlpI GC'TnA_GC
Cuts at: 0 703 2235
Size: 703 1532
Bme1580I G_kGCm'C
Cuts at: 0 104 197 271 534 824 935 1240 1534
Size: 104 93 74 263 290 111 305 294
Cuts at: 1534 1921 2129 2235
Size: 387 208 106
Fragments arranged by size:
387 305 294 290 263 208 111 106
104 93 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 309 1893 2235
Size: 309 1584 342
Fragments arranged by size:
1584 342 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1186 2100 2161 2235
Size: 235 951 914 61 74
Fragments arranged by size:
951 914 235 74 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 352 988 2235
Size: 148 204 636 1247
Fragments arranged by size:
1247 636 204 148
BsaAI yAC'GTr
Cuts at: 0 1623 2235
Size: 1623 612
BsaBI GATnn'nnATC
Cuts at: 0 2062 2235
Size: 2062 173
BsaHI Gr'CG_yC
Cuts at: 0 86 150 520 776 1211 1856 2235
Size: 86 64 370 256 435 645 379
Fragments arranged by size:
645 435 379 370 256 86 64
BsaWI w'CCGG_w
Cuts at: 0 1690 1979 2235
Size: 1690 289 256
Fragments arranged by size:
1690 289 256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 280 619 649 2235
Size: 250 30 339 30 1586
Fragments arranged by size:
1586 339 250 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 1684 2235
Size: 145 1539 551
Fragments arranged by size:
1539 551 145
BseYI C'CCAG_C
Cuts at: 0 199 359 548 1024 1446 1533 1613 2235
Size: 199 160 189 476 422 87 80 622
Fragments arranged by size:
622 476 422 199 189 160 87 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 610 733 967 1126 1671 1684 1789
Size: 594 16 123 234 159 545 13 105
Cuts at: 1789 1885 2235
Size: 96 350
Fragments arranged by size:
594 545 350 234 159 123 105 96
16 13
BsiEI CG_ry'CG
Cuts at: 0 1853 2235
Size: 1853 382
BsiHKAI G_wGCw'C
Cuts at: 0 440 1004 1698 2003 2235
Size: 440 564 694 305 232
Fragments arranged by size:
694 564 440 305 232
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1435 2235
Size: 1435 800
Bsp1286I G_dGCh'C
Cuts at: 0 104 197 271 440 534 544 824 935
Size: 104 93 74 169 94 10 280 111
Cuts at: 935 1004 1240 1534 1698 1921 2003 2075 2129
Size: 69 236 294 164 223 82 72 54
Cuts at: 2129 2235
Size: 106
Fragments arranged by size:
294 280 236 223 169 164 111 106
104 94 93 82 74 72 69 54
10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 344 764 2235
Size: 103 241 420 1471
Fragments arranged by size:
1471 420 241 103
BssSI C'ACGA_G
Cuts at: 0 943 2235
Size: 943 1292
BstAPI GCAn_nnn'nTGC
Cuts at: 0 756 954 2235
Size: 756 198 1281
Fragments arranged by size:
1281 756 198
BstEII G'GTnAC_C
Cuts at: 0 474 2235
Size: 474 1761
BstXI CCAn_nnnn'nTGG
Cuts at: 0 315 594 852 2235
Size: 315 279 258 1383
Fragments arranged by size:
1383 315 279 258
BstYI r'GATC_y
Cuts at: 0 1736 2235
Size: 1736 499
Bsu36I CC'TnA_GG
Cuts at: 0 1400 2197 2235
Size: 1400 797 38
Fragments arranged by size:
1400 797 38
BtgI C'CryG_G
Cuts at: 0 1232 1994 2235
Size: 1232 762 241
Fragments arranged by size:
1232 762 241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2135 2235
Size: 2135 100
BtsI GCAGTG_nn'
Cuts at: 0 1696 1919 2213 2235
Size: 1696 223 294 22
Fragments arranged by size:
1696 294 223 22
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 657 692 2235
Size: 657 35 1543
Fragments arranged by size:
1543 657 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1216 2235
Size: 1216 1019
EaeI y'GGCC_r
Cuts at: 0 305 347 374 416 485 557 785 797
Size: 305 42 27 42 69 72 228 12
Cuts at: 797 854 920 1145 1483 1850 2235
Size: 57 66 225 338 367 385
Fragments arranged by size:
385 367 338 305 228 225 72 69
66 57 42 42 27 12
EagI C'GGCC_G
Cuts at: 0 1850 2235
Size: 1850 385
EarI CTCTTCn'nnn_
Cuts at: 0 2020 2235
Size: 2020 215
EcoICRI GAG'CTC
Cuts at: 0 438 2001 2235
Size: 438 1563 234
Fragments arranged by size:
1563 438 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1186 1198 2100 2161 2235
Size: 235 951 12 902 61 74
Fragments arranged by size:
951 902 235 74 61 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 766 2235
Size: 766 1469
EcoO109I rG'GnC_Cy
Cuts at: 0 462 531 932 939 1065 1161 1716 1874
Size: 462 69 401 7 126 96 555 158
Cuts at: 1874 2201 2235
Size: 327 34
Fragments arranged by size:
555 462 401 327 158 126 96 69
34 7
FspI TGC'GCA
Cuts at: 0 1129 2235
Size: 1129 1106
HaeII r_GCGC'y
Cuts at: 0 89 153 779 1620 2235
Size: 89 64 626 841 615
Fragments arranged by size:
841 626 615 89 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 978 1010 1503 1535 2235
Size: 978 32 493 32 700
Fragments arranged by size:
978 700 493 32 32
HincII GTy'rAC
Cuts at: 0 1215 2235
Size: 1215 1020
KasI G'GCGC_C
Cuts at: 0 85 149 775 2235
Size: 85 64 626 1460
Fragments arranged by size:
1460 626 85 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1608 2235
Size: 1271 337 627
Fragments arranged by size:
1271 627 337
MscI TGG'CCA
Cuts at: 0 307 418 856 922 1147 1485 2235
Size: 307 111 438 66 225 338 750
Fragments arranged by size:
750 438 338 307 225 111 66
MslI CAynn'nnrTG
Cuts at: 0 97 313 493 1607 2155 2235
Size: 97 216 180 1114 548 80
Fragments arranged by size:
1114 548 216 180 97 80
MspA1I CmG'CkG
Cuts at: 0 363 504 552 562 608 1197 1298 1551
Size: 363 141 48 10 46 589 101 253
Cuts at: 1551 1564 2139 2235
Size: 13 575 96
Fragments arranged by size:
589 575 363 253 141 101 96 48
46 13 10
NarI GG'CG_CC
Cuts at: 0 86 150 776 2235
Size: 86 64 626 1459
Fragments arranged by size:
1459 626 86 64
NspI r_CATG'y
Cuts at: 0 702 1259 2229 2235
Size: 702 557 970 6
Fragments arranged by size:
970 702 557 6
PasI CC'CwG_GG
Cuts at: 0 186 1068 2235
Size: 186 882 1167
Fragments arranged by size:
1167 882 186
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 1481 1590 1806 2229 2235
Size: 555 926 109 216 423 6
Fragments arranged by size:
926 555 423 216 109 6
PpuMI rG'GwC_Cy
Cuts at: 0 462 1716 2235
Size: 462 1254 519
Fragments arranged by size:
1254 519 462
PspOMI G'GGCC_C
Cuts at: 0 530 931 2235
Size: 530 401 1304
Fragments arranged by size:
1304 530 401
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1960 1992 2235
Size: 1960 32 243
Fragments arranged by size:
1960 243 32
PstI C_TGCA'G
Cuts at: 0 341 878 1556 1632 2220 2235
Size: 341 537 678 76 588 15
Fragments arranged by size:
678 588 537 341 76 15
PvuII CAG'CTG
Cuts at: 0 363 504 552 1197 2235
Size: 363 141 48 645 1038
Fragments arranged by size:
1038 645 363 141 48
SacI G_AGCT'C
Cuts at: 0 440 2003 2235
Size: 440 1563 232
Fragments arranged by size:
1563 440 232
SanDI GG'GwC_CC
Cuts at: 0 1716 2235
Size: 1716 519
SfcI C'TryA_G
Cuts at: 0 337 874 963 1552 1628 2184 2216 2235
Size: 337 537 89 589 76 556 32 19
Fragments arranged by size:
589 556 537 337 89 76 32 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1411 2235
Size: 1411 824
SfoI GGC'GCC
Cuts at: 0 87 151 777 2235
Size: 87 64 626 1458
Fragments arranged by size:
1458 626 87 64
SmaI CCC'GGG
Cuts at: 0 1714 2235
Size: 1714 521
SmlI C'TyrA_G
Cuts at: 0 163 367 1003 2235
Size: 163 204 636 1232
Fragments arranged by size:
1232 636 204 163
SphI G_CATG'C
Cuts at: 0 702 2235
Size: 702 1533
StuI AGG'CCT
Cuts at: 0 142 2235
Size: 142 2093
StyI C'CwwG_G
Cuts at: 0 204 479 2235
Size: 204 275 1756
Fragments arranged by size:
1756 275 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 325 711 2235
Size: 199 126 386 1524
Fragments arranged by size:
1524 386 199 126
TatI w'GTAC_w
Cuts at: 0 155 1778 2111 2235
Size: 155 1623 333 124
Fragments arranged by size:
1623 333 155 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 250 282 619 651 1688 1720 2235
Size: 250 32 337 32 1037 32 515
Fragments arranged by size:
1037 515 337 250 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1728 2235
Size: 1728 507
XmaI C'CCGG_G
Cuts at: 0 1712 2235
Size: 1712 523
ZraI GAC'GTC
Cuts at: 0 1212 2235
Size: 1212 1023
Enzymes that do cut:
AarI AatII AcuI AhdI AleI AlwNI ApaI AvaI
BaeI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BplI BpmI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NspI
PasI PflMI PpuMI PspOMI PsrI PstI PvuII SacI
SanDI SfcI SfiI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BbsI BbvCI BfrBI BmgBI BmrI BmtI Bpu10I BsaI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BstBI BstZ17I ClaI DraI DraIII EciI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI