Restriction map for
pF1KB3090
(Linear) (Six-base) MAPSORT of: KIBB3090.seq Check: 5466 from: 1 to: 1854
KIBB3090.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 151 1854
Size: 151 1703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1070 1136 1331 1370 1854
Size: 569 501 66 195 39 484
Fragments arranged by size:
569 501 484 195 66 39
AflIII A'CryG_T
Cuts at: 0 1417 1854
Size: 1417 437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 821 1854
Size: 26 795 1033
Fragments arranged by size:
1033 795 26
AlwNI CAG_nnn'CTG
Cuts at: 0 345 686 1854
Size: 345 341 1168
Fragments arranged by size:
1168 345 341
ApoI r'AATT_y
Cuts at: 0 753 1854
Size: 753 1101
AseI AT'TA_AT
Cuts at: 0 1378 1463 1854
Size: 1378 85 391
Fragments arranged by size:
1378 391 85
BamHI G'GATC_C
Cuts at: 0 364 1854
Size: 364 1490
BanI G'GyrC_C
Cuts at: 0 177 1854
Size: 177 1677
BbsI GAAGACnn'nnnn_
Cuts at: 0 506 1854
Size: 506 1348
Bme1580I G_kGCm'C
Cuts at: 0 1788 1854
Size: 1788 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 696 1854
Size: 696 1158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 218 1854
Size: 218 1636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 662 974 1118 1160 1280 1854
Size: 444 218 312 144 42 120 574
Fragments arranged by size:
574 444 312 218 144 120 42
Bpu10I CC'TnA_GC
Cuts at: 0 860 1854
Size: 860 994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 1854
Size: 957 897
BsaI GGTCTCn'nnnn_
Cuts at: 0 1211 1854
Size: 1211 643
BsaWI w'CCGG_w
Cuts at: 0 719 1854
Size: 719 1135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 635 665 1854
Size: 635 30 1189
Fragments arranged by size:
1189 635 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1345 1701 1854
Size: 1345 356 153
Fragments arranged by size:
1345 356 153
BsmBI CGTCTCn'nnnn_
Cuts at: 0 389 1854
Size: 389 1465
Bsp1286I G_dGCh'C
Cuts at: 0 1788 1854
Size: 1788 66
BspHI T'CATG_A
Cuts at: 0 1166 1854
Size: 1166 688
BsrDI GCAATG_nn'
Cuts at: 0 545 906 1854
Size: 545 361 948
Fragments arranged by size:
948 545 361
BssSI C'ACGA_G
Cuts at: 0 203 1854
Size: 203 1651
BstYI r'GATC_y
Cuts at: 0 364 1854
Size: 364 1490
BtgI C'CryG_G
Cuts at: 0 964 1854
Size: 964 890
DraI TTT'AAA
Cuts at: 0 1448 1854
Size: 1448 406
EaeI y'GGCC_r
Cuts at: 0 580 835 1559 1854
Size: 580 255 724 295
Fragments arranged by size:
724 580 295 255
EarI CTCTTCn'nnn_
Cuts at: 0 513 1322 1854
Size: 513 809 532
Fragments arranged by size:
809 532 513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 569 662 974 1070 1118 1136 1160
Size: 444 125 93 312 96 48 18 24
Cuts at: 1160 1280 1331 1370 1854
Size: 120 51 39 484
Fragments arranged by size:
484 444 312 125 120 96 93 51
48 39 24 18
EcoO109I rG'GnC_Cy
Cuts at: 0 34 637 1854
Size: 34 603 1217
Fragments arranged by size:
1217 603 34
EcoRV GAT'ATC
Cuts at: 0 1054 1854
Size: 1054 800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 141 1854
Size: 141 1713
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 1276 1308 1357 1389 1854
Size: 694 32 550 32 49 32 465
Fragments arranged by size:
694 550 465 49 32 32 32
HincII GTy'rAC
Cuts at: 0 1082 1854
Size: 1082 772
MfeI C'AATT_G
Cuts at: 0 1411 1681 1854
Size: 1411 270 173
Fragments arranged by size:
1411 270 173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1669 1854
Size: 1669 185
MscI TGG'CCA
Cuts at: 0 582 837 1854
Size: 582 255 1017
Fragments arranged by size:
1017 582 255
MspA1I CmG'CkG
Cuts at: 0 1074 1854
Size: 1074 780
NcoI C'CATG_G
Cuts at: 0 964 1854
Size: 964 890
NdeI CA'TA_TG
Cuts at: 0 575 1854
Size: 575 1279
NspI r_CATG'y
Cuts at: 0 798 808 979 1421 1854
Size: 798 10 171 442 433
Fragments arranged by size:
798 442 433 171 10
PciI A'CATG_T
Cuts at: 0 1417 1854
Size: 1417 437
PfoI T'CCnGG_A
Cuts at: 0 1433 1854
Size: 1433 421
PpuMI rG'GwC_Cy
Cuts at: 0 34 637 1854
Size: 34 603 1217
Fragments arranged by size:
1217 603 34
PvuII CAG'CTG
Cuts at: 0 1074 1854
Size: 1074 780
ScaI AGT'ACT
Cuts at: 0 546 1854
Size: 546 1308
SexAI A'CCwGG_T
Cuts at: 0 1220 1854
Size: 1220 634
SfcI C'TryA_G
Cuts at: 0 141 327 528 1854
Size: 141 186 201 1326
Fragments arranged by size:
1326 201 186 141
SmlI C'TyrA_G
Cuts at: 0 936 1854
Size: 936 918
SphI G_CATG'C
Cuts at: 0 798 1854
Size: 798 1056
StyI C'CwwG_G
Cuts at: 0 425 964 991 1196 1854
Size: 425 539 27 205 658
Fragments arranged by size:
658 539 425 205 27
TatI w'GTAC_w
Cuts at: 0 544 830 1854
Size: 544 286 1024
Fragments arranged by size:
1024 544 286
Tth111I GACn'n_nGTC
Cuts at: 0 238 1854
Size: 238 1616
XbaI T'CTAG_A
Cuts at: 0 487 759 1854
Size: 487 272 1095
Fragments arranged by size:
1095 487 272
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApoI AseI BamHI
BanI BbsI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaWI BsaXI BseRI BsmBI Bsp1286I BspHI BsrDI
BssSI BstYI BtgI DraI EaeI EarI Eco57MI EcoO109I
EcoRV FalI Hin4I HincII MfeI MmeI MscI MspA1I
NcoI NdeI NspI PciI PfoI PpuMI PvuII ScaI
SexAI SfcI SmlI SphI StyI TatI Tth111I XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI MslI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TstI XcmI
XhoI XmaI XmnI ZraI