Restriction map for
pF1KB3064
(Linear) (Six-base) MAPSORT of: KIBB3064.seq Check: 6734 from: 1 to: 1230
KIBB3064.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 572 902 1230
Size: 362 210 330 328
Fragments arranged by size:
362 330 328 210
AflIII A'CryG_T
Cuts at: 0 1086 1230
Size: 1086 144
AlwNI CAG_nnn'CTG
Cuts at: 0 454 895 1230
Size: 454 441 335
Fragments arranged by size:
454 441 335
ApoI r'AATT_y
Cuts at: 0 232 1230
Size: 232 998
AvaI C'yCGr_G
Cuts at: 0 335 1230
Size: 335 895
BamHI G'GATC_C
Cuts at: 0 940 1230
Size: 940 290
BanI G'GyrC_C
Cuts at: 0 1173 1230
Size: 1173 57
BanII G_rGCy'C
Cuts at: 0 815 855 1230
Size: 815 40 375
Fragments arranged by size:
815 375 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 688 1126 1230
Size: 688 438 104
Fragments arranged by size:
688 438 104
BfrBI ATG'CAT
Cuts at: 0 307 551 842 1115 1230
Size: 307 244 291 273 115
Fragments arranged by size:
307 291 273 244 115
BlpI GC'TnA_GC
Cuts at: 0 809 1230
Size: 809 421
BmrI ACTGGGnnnn_n'
Cuts at: 0 1026 1230
Size: 1026 204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 839 1230
Size: 437 402 391
Fragments arranged by size:
437 402 391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 1230
Size: 615 615
BsaI GGTCTCn'nnnn_
Cuts at: 0 260 375 1230
Size: 260 115 855
Fragments arranged by size:
855 260 115
BsaAI yAC'GTr
Cuts at: 0 712 1024 1230
Size: 712 312 206
Fragments arranged by size:
712 312 206
BsiHKAI G_wGCw'C
Cuts at: 0 815 1230
Size: 815 415
BsmI GAATG_Cn'
Cuts at: 0 553 805 844 1230
Size: 553 252 39 386
Fragments arranged by size:
553 386 252 39
Bsp1286I G_dGCh'C
Cuts at: 0 815 855 1230
Size: 815 40 375
Fragments arranged by size:
815 375 40
BspHI T'CATG_A
Cuts at: 0 1008 1230
Size: 1008 222
BsrGI T'GTAC_A
Cuts at: 0 456 1230
Size: 456 774
BssSI C'ACGA_G
Cuts at: 0 1000 1230
Size: 1000 230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 278 1230
Size: 278 952
BstYI r'GATC_y
Cuts at: 0 892 940 1230
Size: 892 48 290
Fragments arranged by size:
892 290 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1125 1230
Size: 1125 105
DraI TTT'AAA
Cuts at: 0 93 1230
Size: 93 1137
DrdI GACnn_nn'nnGTC
Cuts at: 0 254 1230
Size: 254 976
EaeI y'GGCC_r
Cuts at: 0 567 721 1230
Size: 567 154 509
Fragments arranged by size:
567 509 154
EarI CTCTTCn'nnn_
Cuts at: 0 68 1230
Size: 68 1162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 281 1230
Size: 281 949
EcoICRI GAG'CTC
Cuts at: 0 813 1230
Size: 813 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 437 572 839 902 1230
Size: 362 75 135 267 63 328
Fragments arranged by size:
362 328 267 135 75 63
FspI TGC'GCA
Cuts at: 0 1133 1230
Size: 1133 97
FspAI rTGC'GCAy
Cuts at: 0 1133 1230
Size: 1133 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 1230
Size: 273 32 925
Fragments arranged by size:
925 273 32
HindIII A'AGCT_T
Cuts at: 0 487 1230
Size: 487 743
MluI A'CGCG_T
Cuts at: 0 1086 1230
Size: 1086 144
MslI CAynn'nnrTG
Cuts at: 0 569 827 1230
Size: 569 258 403
Fragments arranged by size:
569 403 258
MspA1I CmG'CkG
Cuts at: 0 482 752 1230
Size: 482 270 478
Fragments arranged by size:
482 478 270
NsiI A_TGCA'T
Cuts at: 0 309 553 844 1117 1230
Size: 309 244 291 273 113
Fragments arranged by size:
309 291 273 244 113
NspI r_CATG'y
Cuts at: 0 832 987 1230
Size: 832 155 243
Fragments arranged by size:
832 243 155
PspXI vC'TCGA_Gb
Cuts at: 0 335 1230
Size: 335 895
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 1230
Size: 1013 32 185
Fragments arranged by size:
1013 185 32
PstI C_TGCA'G
Cuts at: 0 510 1230
Size: 510 720
PvuII CAG'CTG
Cuts at: 0 752 1230
Size: 752 478
SacI G_AGCT'C
Cuts at: 0 815 1230
Size: 815 415
ScaI AGT'ACT
Cuts at: 0 978 1230
Size: 978 252
SfcI C'TryA_G
Cuts at: 0 41 506 1230
Size: 41 465 724
Fragments arranged by size:
724 465 41
SmlI C'TyrA_G
Cuts at: 0 335 630 1230
Size: 335 295 600
Fragments arranged by size:
600 335 295
SnaBI TAC'GTA
Cuts at: 0 712 1230
Size: 712 518
StyI C'CwwG_G
Cuts at: 0 1154 1230
Size: 1154 76
TatI w'GTAC_w
Cuts at: 0 456 976 1230
Size: 456 520 254
Fragments arranged by size:
520 456 254
XhoI C'TCGA_G
Cuts at: 0 335 1230
Size: 335 895
XmnI GAAnn'nnTTC
Cuts at: 0 281 685 1230
Size: 281 404 545
Fragments arranged by size:
545 404 281
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AvaI BamHI BanI BanII
BbsI BfrBI BlpI BmrI BpmI BpuEI BsaI BsaAI
BsiHKAI BsmI Bsp1286I BspHI BsrGI BssSI BstXI BstYI
BtgZI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI
FspI FspAI Hin4I HindIII MluI MslI MspA1I NsiI
NspI PspXI PsrI PstI PvuII SacI ScaI SfcI
SmlI SnaBI StyI TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BglI BglII Bme1580I BmgBI BmtI BplI Bpu10I BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraIII EagI EcoNI EcoO109I EcoRI EcoRV FalI FseI
HaeII HincII HpaI KasI KpnI MfeI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XmaI ZraI