Restriction map for
pF1KB3050
(Linear) (Six-base) MAPSORT of: KIBB3050.seq Check: 2776 from: 1 to: 1620
KIBB3050.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 316 1620
Size: 316 1304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 1620
Size: 594 1026
AflIII A'CryG_T
Cuts at: 0 172 1334 1620
Size: 172 1162 286
Fragments arranged by size:
1162 286 172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 713 1620
Size: 713 907
AlwNI CAG_nnn'CTG
Cuts at: 0 912 925 1620
Size: 912 13 695
Fragments arranged by size:
912 695 13
ApaI G_GGCC'C
Cuts at: 0 61 1320 1620
Size: 61 1259 300
Fragments arranged by size:
1259 300 61
ApoI r'AATT_y
Cuts at: 0 403 1620
Size: 403 1217
AvaI C'yCGr_G
Cuts at: 0 193 459 1433 1620
Size: 193 266 974 187
Fragments arranged by size:
974 266 193 187
BamHI G'GATC_C
Cuts at: 0 165 1620
Size: 165 1455
BanI G'GyrC_C
Cuts at: 0 951 1073 1174 1500 1620
Size: 951 122 101 326 120
Fragments arranged by size:
951 326 122 120 101
BanII G_rGCy'C
Cuts at: 0 61 1217 1320 1405 1620
Size: 61 1156 103 85 215
Fragments arranged by size:
1156 215 103 85 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 943 1127 1620
Size: 943 184 493
Fragments arranged by size:
943 493 184
BbvCI CC'TCA_GC
Cuts at: 0 119 1620
Size: 119 1501
BglI GCCn_nnn'nGGC
Cuts at: 0 130 721 916 1192 1620
Size: 130 591 195 276 428
Fragments arranged by size:
591 428 276 195 130
BlpI GC'TnA_GC
Cuts at: 0 682 1620
Size: 682 938
Bme1580I G_kGCm'C
Cuts at: 0 61 1078 1320 1505 1523 1620
Size: 61 1017 242 185 18 97
Fragments arranged by size:
1017 242 185 97 61 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 438 529 1156 1620
Size: 438 91 627 464
Fragments arranged by size:
627 464 438 91
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 798 1424 1620
Size: 798 626 196
Fragments arranged by size:
798 626 196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 790 1034 1620
Size: 748 42 244 586
Fragments arranged by size:
748 586 244 42
Bpu10I CC'TnA_GC
Cuts at: 0 119 848 1620
Size: 119 729 772
Fragments arranged by size:
772 729 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 970 1620
Size: 112 858 650
Fragments arranged by size:
858 650 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 763 793 1570 1600 1620
Size: 763 30 777 30 20
Fragments arranged by size:
777 763 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 745 787 961 1620
Size: 109 636 42 174 659
Fragments arranged by size:
659 636 174 109 42
BseYI C'CCAG_C
Cuts at: 0 320 579 1390 1620
Size: 320 259 811 230
Fragments arranged by size:
811 320 259 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 580 1620
Size: 207 373 1040
Fragments arranged by size:
1040 373 207
BsiHKAI G_wGCw'C
Cuts at: 0 1020 1370 1620
Size: 1020 350 250
Fragments arranged by size:
1020 350 250
Bsp1286I G_dGCh'C
Cuts at: 0 61 1020 1078 1217 1320 1370 1405 1505
Size: 61 959 58 139 103 50 35 100
Cuts at: 1505 1523 1620
Size: 18 97
Fragments arranged by size:
959 139 103 100 97 61 58 50
35 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 316 1610 1620
Size: 316 1294 10
Fragments arranged by size:
1294 316 10
BsrBI CCG'CTC
Cuts at: 0 110 438 1620
Size: 110 328 1182
Fragments arranged by size:
1182 328 110
BsrDI GCAATG_nn'
Cuts at: 0 1427 1620
Size: 1427 193
BsrFI r'CCGG_y
Cuts at: 0 77 1620
Size: 77 1543
BstYI r'GATC_y
Cuts at: 0 165 1620
Size: 165 1455
BtgI C'CryG_G
Cuts at: 0 20 635 749 1187 1620
Size: 20 615 114 438 433
Fragments arranged by size:
615 438 433 114 20
DraIII CAC_nnn'GTG
Cuts at: 0 450 1620
Size: 450 1170
EaeI y'GGCC_r
Cuts at: 0 17 342 759 816 1184 1193 1485 1620
Size: 17 325 417 57 368 9 292 135
Fragments arranged by size:
417 368 325 292 135 57 17 9
EarI CTCTTCn'nnn_
Cuts at: 0 1393 1620
Size: 1393 227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 904 1620
Size: 904 716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 748 790 1034 1620
Size: 594 154 42 244 586
Fragments arranged by size:
594 586 244 154 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1345 1606 1620
Size: 1345 261 14
Fragments arranged by size:
1345 261 14
EcoO109I rG'GnC_Cy
Cuts at: 0 57 58 99 123 197 735 745 909
Size: 57 1 41 24 74 538 10 164
Cuts at: 909 1316 1620
Size: 407 304
Fragments arranged by size:
538 407 304 164 74 57 41 24
10 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 408 1620
Size: 408 1212
HaeII r_GCGC'y
Cuts at: 0 727 1620
Size: 727 893
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 763 795 1572 1604 1620
Size: 763 32 777 32 16
Fragments arranged by size:
777 763 32 32 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1454 1480 1620
Size: 193 1261 26 140
Fragments arranged by size:
1261 193 140 26
MscI TGG'CCA
Cuts at: 0 19 761 1487 1620
Size: 19 742 726 133
Fragments arranged by size:
742 726 133 19
MslI CAynn'nnrTG
Cuts at: 0 1180 1186 1235 1620
Size: 1180 6 49 385
Fragments arranged by size:
1180 385 49 6
MspA1I CmG'CkG
Cuts at: 0 118 279 320 996 1002 1288 1620
Size: 118 161 41 676 6 286 332
Fragments arranged by size:
676 332 286 161 118 41 6
NaeI GCC'GGC
Cuts at: 0 79 1620
Size: 79 1541
NcoI C'CATG_G
Cuts at: 0 20 635 1620
Size: 20 615 985
Fragments arranged by size:
985 615 20
NgoMIV G'CCGG_C
Cuts at: 0 77 1620
Size: 77 1543
NspI r_CATG'y
Cuts at: 0 176 876 1338 1620
Size: 176 700 462 282
Fragments arranged by size:
700 462 282 176
PasI CC'CwG_GG
Cuts at: 0 41 672 1356 1506 1620
Size: 41 631 684 150 114
Fragments arranged by size:
684 631 150 114 41
PciI A'CATG_T
Cuts at: 0 172 1334 1620
Size: 172 1162 286
Fragments arranged by size:
1162 286 172
PflMI CCAn_nnn'nTGG
Cuts at: 0 768 1234 1411 1494 1620
Size: 768 466 177 83 126
Fragments arranged by size:
768 466 177 126 83
PpuMI rG'GwC_Cy
Cuts at: 0 99 197 1620
Size: 99 98 1423
Fragments arranged by size:
1423 99 98
PspOMI G'GGCC_C
Cuts at: 0 57 1316 1620
Size: 57 1259 304
Fragments arranged by size:
1259 304 57
PstI C_TGCA'G
Cuts at: 0 860 917 1001 1620
Size: 860 57 84 619
Fragments arranged by size:
860 619 84 57
PvuII CAG'CTG
Cuts at: 0 279 996 1002 1288 1620
Size: 279 717 6 286 332
Fragments arranged by size:
717 332 286 279 6
SanDI GG'GwC_CC
Cuts at: 0 197 1620
Size: 197 1423
SapI GCTCTTCn'nnn_
Cuts at: 0 1393 1620
Size: 1393 227
SbfI CC_TGCA'GG
Cuts at: 0 917 1620
Size: 917 703
SfcI C'TryA_G
Cuts at: 0 856 913 997 1620
Size: 856 57 84 623
Fragments arranged by size:
856 623 84 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 130 1192 1620
Size: 130 1062 428
Fragments arranged by size:
1062 428 130
SmaI CCC'GGG
Cuts at: 0 195 461 1620
Size: 195 266 1159
Fragments arranged by size:
1159 266 195
SmlI C'TyrA_G
Cuts at: 0 127 985 1620
Size: 127 858 635
Fragments arranged by size:
858 635 127
SpeI A'CTAG_T
Cuts at: 0 1207 1620
Size: 1207 413
StuI AGG'CCT
Cuts at: 0 715 1620
Size: 715 905
StyI C'CwwG_G
Cuts at: 0 20 635 716 1620
Size: 20 615 81 904
Fragments arranged by size:
904 615 81 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 983 1333 1620
Size: 983 350 287
Fragments arranged by size:
983 350 287
TatI w'GTAC_w
Cuts at: 0 467 483 1025 1620
Size: 467 16 542 595
Fragments arranged by size:
595 542 467 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1570 1602 1620
Size: 1570 32 18
Fragments arranged by size:
1570 32 18
Tth111I GACn'n_nGTC
Cuts at: 0 1423 1620
Size: 1423 197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 642 1185 1620
Size: 642 543 435
Fragments arranged by size:
642 543 435
XmaI C'CCGG_G
Cuts at: 0 193 459 1620
Size: 193 266 1161
Fragments arranged by size:
1161 266 193
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstYI
BtgI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PciI PflMI PpuMI PspOMI
PstI PvuII SanDI SapI SbfI SfcI SfiI SmaI
SmlI SpeI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI ScaI SexAI SfoI SgfI
SgrAI SnaBI SphI SrfI SspI SwaI XbaI XhoI
XmnI ZraI