Restriction map for
pF1KB3047
(Linear) (Six-base) MAPSORT of: KIBB3047.seq Check: 409 from: 1 to: 3441
KIBB3047.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1662 3441
Size: 1662 1779
AccI GT'mk_AC
Cuts at: 0 2899 3041 3441
Size: 2899 142 400
Fragments arranged by size:
2899 400 142
AclI AA'CG_TT
Cuts at: 0 748 1293 3441
Size: 748 545 2148
Fragments arranged by size:
2148 748 545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1127 2108 3441
Size: 1127 981 1333
Fragments arranged by size:
1333 1127 981
AflIII A'CryG_T
Cuts at: 0 2588 2667 3441
Size: 2588 79 774
Fragments arranged by size:
2588 774 79
AleI CACnn'nnGTG
Cuts at: 0 1246 3441
Size: 1246 2195
AlwNI CAG_nnn'CTG
Cuts at: 0 2931 3125 3441
Size: 2931 194 316
Fragments arranged by size:
2931 316 194
ApaI G_GGCC'C
Cuts at: 0 2539 3441
Size: 2539 902
ApaLI G'TGCA_C
Cuts at: 0 2462 3441
Size: 2462 979
ApoI r'AATT_y
Cuts at: 0 13 290 1710 1746 1897 2195 2231 3441
Size: 13 277 1420 36 151 298 36 1210
Fragments arranged by size:
1420 1210 298 277 151 36 36 13
AseI AT'TA_AT
Cuts at: 0 2206 3441
Size: 2206 1235
AvaI C'yCGr_G
Cuts at: 0 2321 3441
Size: 2321 1120
AvrII C'CTAG_G
Cuts at: 0 850 3441
Size: 850 2591
BamHI G'GATC_C
Cuts at: 0 303 3159 3441
Size: 303 2856 282
Fragments arranged by size:
2856 303 282
BanII G_rGCy'C
Cuts at: 0 985 1186 2539 3410 3441
Size: 985 201 1353 871 31
Fragments arranged by size:
1353 985 871 201 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 2785 3101 3441
Size: 2785 316 340
Fragments arranged by size:
2785 340 316
BbvCI CC'TCA_GC
Cuts at: 0 1679 3441
Size: 1679 1762
BciVI GTATCCnnnnn_n'
Cuts at: 0 1996 3441
Size: 1996 1445
BclI T'GATC_A
Cuts at: 0 3353 3441
Size: 3353 88
BglII A'GATC_T
Cuts at: 0 388 526 2610 3441
Size: 388 138 2084 831
Fragments arranged by size:
2084 831 388 138
Bme1580I G_kGCm'C
Cuts at: 0 2466 2539 3441
Size: 2466 73 902
Fragments arranged by size:
2466 902 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 2853 3441
Size: 2853 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1064 1273 1528 2372 2591 2808 2852
Size: 664 400 209 255 844 219 217 44
Cuts at: 2852 2946 3441
Size: 94 495
Fragments arranged by size:
844 664 495 400 255 219 217 209
94 44
Bpu10I CC'TnA_GC
Cuts at: 0 1679 3441
Size: 1679 1762
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1768 3441
Size: 1768 1673
BsaAI yAC'GTr
Cuts at: 0 2013 2670 3441
Size: 2013 657 771
Fragments arranged by size:
2013 771 657
BsaBI GATnn'nnATC
Cuts at: 0 2261 3441
Size: 2261 1180
BsaWI w'CCGG_w
Cuts at: 0 2771 3441
Size: 2771 670
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 1041 1071 1826 1856 3441
Size: 637 30 374 30 755 30 1585
Fragments arranged by size:
1585 755 637 374 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 1696 2590 3441
Size: 235 1461 894 851
Fragments arranged by size:
1461 894 851 235
BseYI C'CCAG_C
Cuts at: 0 2883 3125 3441
Size: 2883 242 316
Fragments arranged by size:
2883 316 242
BsiHKAI G_wGCw'C
Cuts at: 0 985 2466 3441
Size: 985 1481 975
Fragments arranged by size:
1481 985 975
BsmI GAATG_Cn'
Cuts at: 0 2028 3441
Size: 2028 1413
BsmBI CGTCTCn'nnnn_
Cuts at: 0 217 3441
Size: 217 3224
Bsp1286I G_dGCh'C
Cuts at: 0 985 1186 2466 2539 3410 3441
Size: 985 201 1280 73 871 31
Fragments arranged by size:
1280 985 871 201 73 31
BspEI T'CCGG_A
Cuts at: 0 2771 3441
Size: 2771 670
BspHI T'CATG_A
Cuts at: 0 2622 3441
Size: 2622 819
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 222 1662 3441
Size: 222 1440 1779
Fragments arranged by size:
1779 1440 222
BsrDI GCAATG_nn'
Cuts at: 0 2184 2259 3441
Size: 2184 75 1182
Fragments arranged by size:
2184 1182 75
BsrGI T'GTAC_A
Cuts at: 0 3015 3441
Size: 3015 426
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2712 3441
Size: 2712 729
BstBI TT'CG_AA
Cuts at: 0 3433 3441
Size: 3433 8
BstEII G'GTnAC_C
Cuts at: 0 1807 2749 3441
Size: 1807 942 692
Fragments arranged by size:
1807 942 692
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1179 3441
Size: 1179 2262
BstYI r'GATC_y
Cuts at: 0 303 388 526 2610 2768 3159 3441
Size: 303 85 138 2084 158 391 282
Fragments arranged by size:
2084 391 303 282 158 138 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 591 2759 3441
Size: 591 2168 682
Fragments arranged by size:
2168 682 591
BtsI GCAGTG_nn'
Cuts at: 0 1734 3441
Size: 1734 1707
DraI TTT'AAA
Cuts at: 0 101 1929 2364 2375 3441
Size: 101 1828 435 11 1066
Fragments arranged by size:
1828 1066 435 101 11
EarI CTCTTCn'nnn_
Cuts at: 0 662 936 971 2127 2650 3441
Size: 662 274 35 1156 523 791
Fragments arranged by size:
1156 791 662 523 274 35
EcoICRI GAG'CTC
Cuts at: 0 983 3441
Size: 983 2458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1064 1127 1273 1528 2108 2372 2591
Size: 664 400 63 146 255 580 264 219
Cuts at: 2591 2808 2852 2946 3441
Size: 217 44 94 495
Fragments arranged by size:
664 580 495 400 264 255 219 217
146 94 63 44
EcoO109I rG'GnC_Cy
Cuts at: 0 1168 1948 2928 3441
Size: 1168 780 980 513
Fragments arranged by size:
1168 980 780 513
EcoRI G'AATT_C
Cuts at: 0 2195 3441
Size: 2195 1246
EcoRV GAT'ATC
Cuts at: 0 2427 2526 3441
Size: 2427 99 915
Fragments arranged by size:
2427 915 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3226 3441
Size: 3226 215
FspI TGC'GCA
Cuts at: 0 2804 3441
Size: 2804 637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 831 863 1041 1073 1264 1296
Size: 199 32 600 32 178 32 191 32
Cuts at: 1296 1903 1935 2265 2297 2370 2402 2977 3009
Size: 607 32 330 32 73 32 575 32
Cuts at: 3009 3295 3327 3441
Size: 286 32 114
Fragments arranged by size:
607 600 575 330 286 199 191 178
114 73 32 32 32 32 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1139 3441
Size: 1139 2302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 3441
Size: 674 2767
MslI CAynn'nnrTG
Cuts at: 0 662 1246 2668 3441
Size: 662 584 1422 773
Fragments arranged by size:
1422 773 662 584
MspA1I CmG'CkG
Cuts at: 0 910 2569 3125 3441
Size: 910 1659 556 316
Fragments arranged by size:
1659 910 556 316
NdeI CA'TA_TG
Cuts at: 0 2740 2942 3441
Size: 2740 202 499
Fragments arranged by size:
2740 499 202
NspI r_CATG'y
Cuts at: 0 2143 2592 3441
Size: 2143 449 849
Fragments arranged by size:
2143 849 449
PciI A'CATG_T
Cuts at: 0 2588 3441
Size: 2588 853
PfoI T'CCnGG_A
Cuts at: 0 2651 2829 3441
Size: 2651 178 612
Fragments arranged by size:
2651 612 178
PmlI CAC'GTG
Cuts at: 0 2670 3441
Size: 2670 771
PpuMI rG'GwC_Cy
Cuts at: 0 1168 2928 3441
Size: 1168 1760 513
Fragments arranged by size:
1760 1168 513
PsiI TTA'TAA
Cuts at: 0 989 3441
Size: 989 2452
PspOMI G'GGCC_C
Cuts at: 0 2535 3441
Size: 2535 906
PvuII CAG'CTG
Cuts at: 0 910 2569 3125 3441
Size: 910 1659 556 316
Fragments arranged by size:
1659 910 556 316
SacI G_AGCT'C
Cuts at: 0 985 3441
Size: 985 2456
ScaI AGT'ACT
Cuts at: 0 494 3441
Size: 494 2947
SexAI A'CCwGG_T
Cuts at: 0 282 3441
Size: 282 3159
SfcI C'TryA_G
Cuts at: 0 843 2678 2900 3335 3441
Size: 843 1835 222 435 106
Fragments arranged by size:
1835 843 435 222 106
SmlI C'TyrA_G
Cuts at: 0 1783 3441
Size: 1783 1658
StuI AGG'CCT
Cuts at: 0 1226 3441
Size: 1226 2215
StyI C'CwwG_G
Cuts at: 0 850 938 1172 1391 1533 3164 3441
Size: 850 88 234 219 142 1631 277
Fragments arranged by size:
1631 850 277 234 219 142 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 498 1746 3441
Size: 498 1248 1695
Fragments arranged by size:
1695 1248 498
TatI w'GTAC_w
Cuts at: 0 492 3015 3048 3441
Size: 492 2523 33 393
Fragments arranged by size:
2523 492 393 33
XbaI T'CTAG_A
Cuts at: 0 3244 3441
Size: 3244 197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 951 1980 3441
Size: 951 1029 1461
Fragments arranged by size:
1461 1029 951
XmnI GAAnn'nnTTC
Cuts at: 0 3429 3441
Size: 3429 12
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AvaI AvrII BamHI BanII BbsI
BbvCI BciVI BclI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI
BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI DraI
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
Hin4I HindIII MmeI MslI MspA1I NdeI NspI PciI
PfoI PmlI PpuMI PsiI PspOMI PvuII SacI ScaI
SexAI SfcI SmlI StuI StyI TaqII TatI XbaI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AlfI AloI
AscI AsiSI BaeI BanI BbeI BcgI BfrBI BglI
BlpI BmgBI BmtI BplI BsaI BsaHI BsgI BsiEI
BsiWI BsrBI BsrFI BssHII BssSI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoNI
FseI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PpiI PshAI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI
XmaI ZraI