Restriction map for
pF1KB3046
(Linear) (Six-base) MAPSORT of: KIBB3046.seq Check: 1740 from: 1 to: 4521
KIBB3046.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3607 4521
Size: 3607 914
AccI GT'mk_AC
Cuts at: 0 689 1466 4521
Size: 689 777 3055
Fragments arranged by size:
3055 777 689
Acc65I G'GTAC_C
Cuts at: 0 257 550 4521
Size: 257 293 3971
Fragments arranged by size:
3971 293 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 761 1613 1762 3337 4463 4521
Size: 82 679 852 149 1575 1126 58
Fragments arranged by size:
1575 1126 852 679 149 82 58
AflIII A'CryG_T
Cuts at: 0 2566 2603 3603 4521
Size: 2566 37 1000 918
Fragments arranged by size:
2566 1000 918 37
AhdI GACnn_n'nnGTC
Cuts at: 0 2604 4521
Size: 2604 1917
AleI CACnn'nnGTG
Cuts at: 0 676 2000 3862 4240 4521
Size: 676 1324 1862 378 281
Fragments arranged by size:
1862 1324 676 378 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4520 4521
Size: 4520 1
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2266 2298 2737 2769 4521
Size: 2266 32 439 32 1752
Fragments arranged by size:
2266 1752 439 32 32
ApaI G_GGCC'C
Cuts at: 0 3540 4521
Size: 3540 981
ApaLI G'TGCA_C
Cuts at: 0 745 4521
Size: 745 3776
ApoI r'AATT_y
Cuts at: 0 109 571 615 1339 1877 2992 3370 3469
Size: 109 462 44 724 538 1115 378 99
Cuts at: 3469 3523 4351 4521
Size: 54 828 170
Fragments arranged by size:
1115 828 724 538 462 378 170 109
99 54 44
AvaI C'yCGr_G
Cuts at: 0 1164 1274 3539 3627 4521
Size: 1164 110 2265 88 894
Fragments arranged by size:
2265 1164 894 110 88
AvrII C'CTAG_G
Cuts at: 0 2247 4521
Size: 2247 2274
BamHI G'GATC_C
Cuts at: 0 600 4521
Size: 600 3921
BanI G'GyrC_C
Cuts at: 0 257 550 934 3330 4521
Size: 257 293 384 2396 1191
Fragments arranged by size:
2396 1191 384 293 257
BanII G_rGCy'C
Cuts at: 0 339 2702 2789 3540 4521
Size: 339 2363 87 751 981
Fragments arranged by size:
2363 981 751 339 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 70 1661 2296 3052 4521
Size: 70 1591 635 756 1469
Fragments arranged by size:
1591 1469 756 635 70
BbvCI CC'TCA_GC
Cuts at: 0 626 1846 2812 3278 4521
Size: 626 1220 966 466 1243
Fragments arranged by size:
1243 1220 966 626 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1706 1740 4441 4475 4521
Size: 1706 34 2701 34 46
Fragments arranged by size:
2701 1706 46 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1452 2292 3028 4521
Size: 1452 840 736 1493
Fragments arranged by size:
1493 1452 840 736
BfrBI ATG'CAT
Cuts at: 0 1116 4521
Size: 1116 3405
BglI GCCn_nnn'nGGC
Cuts at: 0 2093 4521
Size: 2093 2428
BglII A'GATC_T
Cuts at: 0 2532 4521
Size: 2532 1989
Bme1580I G_kGCm'C
Cuts at: 0 749 3253 3540 4521
Size: 749 2504 287 981
Fragments arranged by size:
2504 981 749 287
BmrI ACTGGGnnnn_n'
Cuts at: 0 373 1762 1804 3502 4234 4521
Size: 373 1389 42 1698 732 287
Fragments arranged by size:
1698 1389 732 373 287 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1612 3303 3818 4521
Size: 1612 1691 515 703
Fragments arranged by size:
1691 1612 703 515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1355 4037 4521
Size: 1355 2682 484
Fragments arranged by size:
2682 1355 484
Bpu10I CC'TnA_GC
Cuts at: 0 626 1809 1846 2812 3278 3326 4521
Size: 626 1183 37 966 466 48 1195
Fragments arranged by size:
1195 1183 966 626 466 48 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1343 1582 3813 4241 4521
Size: 1343 239 2231 428 280
Fragments arranged by size:
2231 1343 428 280 239
BsaBI GATnn'nnATC
Cuts at: 0 799 4114 4521
Size: 799 3315 407
Fragments arranged by size:
3315 799 407
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 728 758 874 904 2737 2767 2800 2830
Size: 728 30 116 30 1833 30 33 30
Cuts at: 2830 3866 3896 4521
Size: 1036 30 625
Fragments arranged by size:
1833 1036 728 625 116 33 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 749 2452 4399 4521
Size: 749 1703 1947 122
Fragments arranged by size:
1947 1703 749 122
BseYI C'CCAG_C
Cuts at: 0 822 2345 4273 4521
Size: 822 1523 1928 248
Fragments arranged by size:
1928 1523 822 248
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1162 4471 4521
Size: 77 1085 3309 50
Fragments arranged by size:
3309 1085 77 50
BsiHKAI G_wGCw'C
Cuts at: 0 339 749 1598 3085 3506 4521
Size: 339 410 849 1487 421 1015
Fragments arranged by size:
1487 1015 849 421 410 339
BsmI GAATG_Cn'
Cuts at: 0 527 2113 2172 2224 4521
Size: 527 1586 59 52 2297
Fragments arranged by size:
2297 1586 527 59 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2055 2503 4259 4521
Size: 2055 448 1756 262
Fragments arranged by size:
2055 1756 448 262
Bsp1286I G_dGCh'C
Cuts at: 0 339 749 1598 2702 2789 3085 3253 3506
Size: 339 410 849 1104 87 296 168 253
Cuts at: 3506 3540 4521
Size: 34 981
Fragments arranged by size:
1104 981 849 410 339 296 253 168
87 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3607 4521
Size: 3607 914
BsrDI GCAATG_nn'
Cuts at: 0 478 1415 2228 2579 4073 4521
Size: 478 937 813 351 1494 448
Fragments arranged by size:
1494 937 813 478 448 351
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1267 2230 2242 4521
Size: 1267 963 12 2279
Fragments arranged by size:
2279 1267 963 12
BstEII G'GTnAC_C
Cuts at: 0 1158 2328 4425 4521
Size: 1158 1170 2097 96
Fragments arranged by size:
2097 1170 1158 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 678 1440 4242 4247 4521
Size: 678 762 2802 5 274
Fragments arranged by size:
2802 762 678 274 5
BstYI r'GATC_y
Cuts at: 0 600 1354 2349 2532 4521
Size: 600 754 995 183 1989
Fragments arranged by size:
1989 995 754 600 183
BtgI C'CryG_G
Cuts at: 0 956 1985 2940 4190 4521
Size: 956 1029 955 1250 331
Fragments arranged by size:
1250 1029 956 955 331
BtsI GCAGTG_nn'
Cuts at: 0 1724 2991 3025 4521
Size: 1724 1267 34 1496
Fragments arranged by size:
1724 1496 1267 34
ClaI AT'CG_AT
Cuts at: 0 1726 4521
Size: 1726 2795
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3365 3400 4521
Size: 3365 35 1121
Fragments arranged by size:
3365 1121 35
DraI TTT'AAA
Cuts at: 0 2675 4521
Size: 2675 1846
DraIII CAC_nnn'GTG
Cuts at: 0 1798 3510 4521
Size: 1798 1712 1011
Fragments arranged by size:
1798 1712 1011
DrdI GACnn_nn'nnGTC
Cuts at: 0 1951 4521
Size: 1951 2570
EaeI y'GGCC_r
Cuts at: 0 204 308 959 1548 2518 3854 4132 4249
Size: 204 104 651 589 970 1336 278 117
Cuts at: 4249 4521
Size: 272
Fragments arranged by size:
1336 970 651 589 278 272 204 117
104
EarI CTCTTCn'nnn_
Cuts at: 0 3113 3473 4287 4521
Size: 3113 360 814 234
Fragments arranged by size:
3113 814 360 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 706 1773 4521
Size: 706 1067 2748
Fragments arranged by size:
2748 1067 706
EcoICRI GAG'CTC
Cuts at: 0 337 4521
Size: 337 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 761 1355 1613 1762 3337 4037 4463
Size: 82 679 594 258 149 1575 700 426
Cuts at: 4463 4521
Size: 58
Fragments arranged by size:
1575 700 679 594 426 258 149 82
58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3478 4521
Size: 3478 1043
EcoO109I rG'GnC_Cy
Cuts at: 0 3536 4521
Size: 3536 985
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 284 316 2590 2622 4521
Size: 110 32 142 32 2274 32 1899
Fragments arranged by size:
2274 1899 142 110 32 32 32
HincII GTy'rAC
Cuts at: 0 1089 3034 3840 4521
Size: 1089 1945 806 681
Fragments arranged by size:
1945 1089 806 681
HindIII A'AGCT_T
Cuts at: 0 2453 4521
Size: 2453 2068
KpnI G_GTAC'C
Cuts at: 0 261 554 4521
Size: 261 293 3967
Fragments arranged by size:
3967 293 261
MfeI C'AATT_G
Cuts at: 0 1947 4521
Size: 1947 2574
MluI A'CGCG_T
Cuts at: 0 2566 4521
Size: 2566 1955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1885 3168 3925 4521
Size: 1885 1283 757 596
Fragments arranged by size:
1885 1283 757 596
MscI TGG'CCA
Cuts at: 0 206 310 961 1550 2520 3856 4521
Size: 206 104 651 589 970 1336 665
Fragments arranged by size:
1336 970 665 651 589 206 104
MslI CAynn'nnrTG
Cuts at: 0 520 676 778 1034 2000 2161 3643 3850
Size: 520 156 102 256 966 161 1482 207
Cuts at: 3850 3862 4240 4521
Size: 12 378 281
Fragments arranged by size:
1482 966 520 378 281 256 207 161
156 102 12
MspA1I CmG'CkG
Cuts at: 0 1665 2345 3301 4387 4521
Size: 1665 680 956 1086 134
Fragments arranged by size:
1665 1086 956 680 134
NcoI C'CATG_G
Cuts at: 0 956 1985 2940 4521
Size: 956 1029 955 1581
Fragments arranged by size:
1581 1029 956 955
NsiI A_TGCA'T
Cuts at: 0 1118 4521
Size: 1118 3403
NspI r_CATG'y
Cuts at: 0 2607 3167 3607 4521
Size: 2607 560 440 914
Fragments arranged by size:
2607 914 560 440
PciI A'CATG_T
Cuts at: 0 2603 3603 4521
Size: 2603 1000 918
Fragments arranged by size:
2603 1000 918
PflMI CCAn_nnn'nTGG
Cuts at: 0 450 892 1881 4521
Size: 450 442 989 2640
Fragments arranged by size:
2640 989 450 442
PfoI T'CCnGG_A
Cuts at: 0 167 2284 4521
Size: 167 2117 2237
Fragments arranged by size:
2237 2117 167
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2737 2769 4521
Size: 2737 32 1752
Fragments arranged by size:
2737 1752 32
PspOMI G'GGCC_C
Cuts at: 0 3536 4521
Size: 3536 985
PstI C_TGCA'G
Cuts at: 0 2868 3266 4521
Size: 2868 398 1255
Fragments arranged by size:
2868 1255 398
PvuII CAG'CTG
Cuts at: 0 1665 3301 4387 4521
Size: 1665 1636 1086 134
Fragments arranged by size:
1665 1636 1086 134
SacI G_AGCT'C
Cuts at: 0 339 4521
Size: 339 4182
SfcI C'TryA_G
Cuts at: 0 478 686 2477 2864 3262 4491 4521
Size: 478 208 1791 387 398 1229 30
Fragments arranged by size:
1791 1229 478 398 387 208 30
SmaI CCC'GGG
Cuts at: 0 1166 4521
Size: 1166 3355
SmlI C'TyrA_G
Cuts at: 0 1358 1597 3792 4256 4521
Size: 1358 239 2195 464 265
Fragments arranged by size:
2195 1358 464 265 239
SpeI A'CTAG_T
Cuts at: 0 1263 4521
Size: 1263 3258
SspI AAT'ATT
Cuts at: 0 379 4521
Size: 379 4142
StuI AGG'CCT
Cuts at: 0 1858 3408 4521
Size: 1858 1550 1113
Fragments arranged by size:
1858 1550 1113
StyI C'CwwG_G
Cuts at: 0 638 956 1040 1985 2247 2585 2781 2836
Size: 638 318 84 945 262 338 196 55
Cuts at: 2836 2940 3128 3334 3669 3860 4143 4521
Size: 104 188 206 335 191 283 378
Fragments arranged by size:
945 638 378 338 335 318 283 262
206 196 191 188 104 84 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1166 1534 1786 1978 2092 2486 3521 4521
Size: 1166 368 252 192 114 394 1035 1000
Fragments arranged by size:
1166 1035 1000 394 368 252 192 114
TatI w'GTAC_w
Cuts at: 0 901 3110 4499 4521
Size: 901 2209 1389 22
Fragments arranged by size:
2209 1389 901 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 149 181 728 760 2322 2354 2936 2968
Size: 149 32 547 32 1562 32 582 32
Cuts at: 2968 4521
Size: 1553
Fragments arranged by size:
1562 1553 582 547 149 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1878 4020 4521
Size: 1878 2142 501
Fragments arranged by size:
2142 1878 501
XmaI C'CCGG_G
Cuts at: 0 1164 4521
Size: 1164 3357
XmnI GAAnn'nnTTC
Cuts at: 0 491 1928 3469 4043 4521
Size: 491 1437 1541 574 478
Fragments arranged by size:
1541 1437 574 491 478
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AloI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BfrBI BglI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I HincII HindIII KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NsiI NspI
PciI PflMI PfoI PpiI PspOMI PstI PvuII SacI
SfcI SmaI SmlI SpeI SspI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AlwNI AscI AseI
AsiSI BaeI BbeI BclI BlpI BmgBI BmtI BsaI
BsaAI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
EagI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SphI SrfI SwaI Tth111I XbaI XhoI ZraI