Restriction map for
pF1KB3033
(Linear) (Six-base) MAPSORT of: KIBB3033.seq Check: 7750 from: 1 to: 1689
KIBB3033.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 879 1689
Size: 879 810
AatII G_ACGT'C
Cuts at: 0 226 1466 1627 1689
Size: 226 1240 161 62
Fragments arranged by size:
1240 226 161 62
AccI GT'mk_AC
Cuts at: 0 734 1370 1689
Size: 734 636 319
Fragments arranged by size:
734 636 319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 190 373 406 1689
Size: 109 81 183 33 1283
Fragments arranged by size:
1283 183 109 81 33
AflIII A'CryG_T
Cuts at: 0 1676 1689
Size: 1676 13
AgeI A'CCGG_T
Cuts at: 0 908 1689
Size: 908 781
AleI CACnn'nnGTG
Cuts at: 0 836 1483 1689
Size: 836 647 206
Fragments arranged by size:
836 647 206
AlwNI CAG_nnn'CTG
Cuts at: 0 903 1689
Size: 903 786
ApaI G_GGCC'C
Cuts at: 0 375 800 1569 1603 1689
Size: 375 425 769 34 86
Fragments arranged by size:
769 425 375 86 34
ApaLI G'TGCA_C
Cuts at: 0 967 1689
Size: 967 722
AvaI C'yCGr_G
Cuts at: 0 567 1568 1689
Size: 567 1001 121
Fragments arranged by size:
1001 567 121
BanI G'GyrC_C
Cuts at: 0 12 663 1689
Size: 12 651 1026
Fragments arranged by size:
1026 651 12
BanII G_rGCy'C
Cuts at: 0 107 375 800 1292 1325 1569 1603 1689
Size: 107 268 425 492 33 244 34 86
Fragments arranged by size:
492 425 268 244 107 86 34 33
BbeI G_GCGC'C
Cuts at: 0 16 1689
Size: 16 1673
BbvCI CC'TCA_GC
Cuts at: 0 1489 1689
Size: 1489 200
BglI GCCn_nnn'nGGC
Cuts at: 0 319 718 1689
Size: 319 399 971
Fragments arranged by size:
971 399 319
BglII A'GATC_T
Cuts at: 0 377 1340 1689
Size: 377 963 349
Fragments arranged by size:
963 377 349
BlpI GC'TnA_GC
Cuts at: 0 1014 1689
Size: 1014 675
Bme1580I G_kGCm'C
Cuts at: 0 375 566 666 800 866 971 1569 1603
Size: 375 191 100 134 66 105 598 34
Cuts at: 1603 1689
Size: 86
Fragments arranged by size:
598 375 191 134 105 100 86 66
34
BmgBI CAC'GTC
Cuts at: 0 231 984 1689
Size: 231 753 705
Fragments arranged by size:
753 705 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 695 1689
Size: 695 994
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 619 956 1126 1231 1689
Size: 559 60 337 170 105 458
Fragments arranged by size:
559 458 337 170 105 60
Bpu10I CC'TnA_GC
Cuts at: 0 134 1489 1689
Size: 134 1355 200
Fragments arranged by size:
1355 200 134
BsaAI yAC'GTr
Cuts at: 0 1065 1689
Size: 1065 624
BsaHI Gr'CG_yC
Cuts at: 0 13 223 1463 1624 1689
Size: 13 210 1240 161 65
Fragments arranged by size:
1240 210 161 65 13
BsaWI w'CCGG_w
Cuts at: 0 509 908 1689
Size: 509 399 781
Fragments arranged by size:
781 509 399
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 1207 1237 1689
Size: 67 30 1110 30 452
Fragments arranged by size:
1110 452 67 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 72 226 1123 1215 1351 1689
Size: 72 154 897 92 136 338
Fragments arranged by size:
897 338 154 136 92 72
BseYI C'CCAG_C
Cuts at: 0 1231 1689
Size: 1231 458
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1489 1689
Size: 253 1236 200
Fragments arranged by size:
1236 253 200
BsiEI CG_ry'CG
Cuts at: 0 980 1689
Size: 980 709
BsiHKAI G_wGCw'C
Cuts at: 0 574 833 971 1054 1115 1217 1689
Size: 574 259 138 83 61 102 472
Fragments arranged by size:
574 472 259 138 102 83 61
BsmI GAATG_Cn'
Cuts at: 0 1500 1689
Size: 1500 189
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1063 1387 1689
Size: 1063 324 302
Fragments arranged by size:
1063 324 302
Bsp1286I G_dGCh'C
Cuts at: 0 107 375 566 574 666 800 833 866
Size: 107 268 191 8 92 134 33 33
Cuts at: 866 971 1054 1115 1217 1292 1325 1569 1603
Size: 105 83 61 102 75 33 244 34
Cuts at: 1603 1689
Size: 86
Fragments arranged by size:
268 244 191 134 107 105 102 92
86 83 75 61 34 33 33 33
8
BspHI T'CATG_A
Cuts at: 0 1328 1689
Size: 1328 361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 879 1623 1689
Size: 879 744 66
Fragments arranged by size:
879 744 66
BsrBI CCG'CTC
Cuts at: 0 974 1001 1013 1689
Size: 974 27 12 676
Fragments arranged by size:
974 676 27 12
BsrFI r'CCGG_y
Cuts at: 0 9 58 666 908 1501 1689
Size: 9 49 608 242 593 188
Fragments arranged by size:
608 593 242 188 49 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 441 1689
Size: 441 1248
BstYI r'GATC_y
Cuts at: 0 377 425 1340 1689
Size: 377 48 915 349
Fragments arranged by size:
915 377 349 48
Bsu36I CC'TnA_GG
Cuts at: 0 1316 1689
Size: 1316 373
BtgI C'CryG_G
Cuts at: 0 258 440 1271 1689
Size: 258 182 831 418
Fragments arranged by size:
831 418 258 182
BtsI GCAGTG_nn'
Cuts at: 0 635 707 864 1175 1448 1689
Size: 635 72 157 311 273 241
Fragments arranged by size:
635 311 273 241 157 72
DraIII CAC_nnn'GTG
Cuts at: 0 966 1689
Size: 966 723
EaeI y'GGCC_r
Cuts at: 0 95 272 614 719 977 1032 1081 1247
Size: 95 177 342 105 258 55 49 166
Cuts at: 1247 1689
Size: 442
Fragments arranged by size:
442 342 258 177 166 105 95 55
49
EagI C'GGCC_G
Cuts at: 0 977 1689
Size: 977 712
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 13 962 1689
Size: 13 949 727
Fragments arranged by size:
949 727 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 190 373 406 559 619 956 1126
Size: 109 81 183 33 153 60 337 170
Cuts at: 1126 1231 1689
Size: 105 458
Fragments arranged by size:
458 337 183 170 153 109 105 81
60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 151 748 1689
Size: 151 597 941
Fragments arranged by size:
941 597 151
EcoO109I rG'GnC_Cy
Cuts at: 0 165 348 797 1004 1154 1311 1565 1599
Size: 165 183 449 207 150 157 254 34
Cuts at: 1599 1689
Size: 90
Fragments arranged by size:
449 254 207 183 165 157 150 90
34
FseI GG_CCGG'CC
Cuts at: 0 62 1689
Size: 62 1627
HaeII r_GCGC'y
Cuts at: 0 16 1689
Size: 16 1673
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1689
Size: 1168 32 489
Fragments arranged by size:
1168 489 32
HincII GTy'rAC
Cuts at: 0 1410 1689
Size: 1410 279
KasI G'GCGC_C
Cuts at: 0 12 1689
Size: 12 1677
MluI A'CGCG_T
Cuts at: 0 1676 1689
Size: 1676 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 1099 1689
Size: 206 893 590
Fragments arranged by size:
893 590 206
MscI TGG'CCA
Cuts at: 0 97 274 721 1034 1689
Size: 97 177 447 313 655
Fragments arranged by size:
655 447 313 177 97
MslI CAynn'nnrTG
Cuts at: 0 559 712 836 1483 1689
Size: 559 153 124 647 206
Fragments arranged by size:
647 559 206 153 124
MspA1I CmG'CkG
Cuts at: 0 490 790 1404 1689
Size: 490 300 614 285
Fragments arranged by size:
614 490 300 285
NaeI GCC'GGC
Cuts at: 0 11 60 1503 1689
Size: 11 49 1443 186
Fragments arranged by size:
1443 186 49 11
NarI GG'CG_CC
Cuts at: 0 13 1689
Size: 13 1676
NcoI C'CATG_G
Cuts at: 0 258 440 1689
Size: 258 182 1249
Fragments arranged by size:
1249 258 182
NgoMIV G'CCGG_C
Cuts at: 0 9 58 1501 1689
Size: 9 49 1443 188
Fragments arranged by size:
1443 188 49 9
NspI r_CATG'y
Cuts at: 0 402 1686 1689
Size: 402 1284 3
Fragments arranged by size:
1284 402 3
PasI CC'CwG_GG
Cuts at: 0 1659 1689
Size: 1659 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 793 903 1689
Size: 793 110 786
Fragments arranged by size:
793 786 110
PpuMI rG'GwC_Cy
Cuts at: 0 165 348 1004 1154 1311 1689
Size: 165 183 656 150 157 378
Fragments arranged by size:
656 378 183 165 157 150
PspOMI G'GGCC_C
Cuts at: 0 371 796 1565 1599 1689
Size: 371 425 769 34 90
Fragments arranged by size:
769 425 371 90 34
PstI C_TGCA'G
Cuts at: 0 489 1143 1170 1403 1689
Size: 489 654 27 233 286
Fragments arranged by size:
654 489 286 233 27
PvuII CAG'CTG
Cuts at: 0 790 1404 1689
Size: 790 614 285
Fragments arranged by size:
790 614 285
SanDI GG'GwC_CC
Cuts at: 0 1004 1689
Size: 1004 685
SfcI C'TryA_G
Cuts at: 0 485 1139 1166 1399 1689
Size: 485 654 27 233 290
Fragments arranged by size:
654 485 290 233 27
SfoI GGC'GCC
Cuts at: 0 14 1689
Size: 14 1675
StuI AGG'CCT
Cuts at: 0 157 1689
Size: 157 1532
StyI C'CwwG_G
Cuts at: 0 258 440 1689
Size: 258 182 1249
Fragments arranged by size:
1249 258 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1245 1689
Size: 1245 444
TatI w'GTAC_w
Cuts at: 0 1353 1385 1689
Size: 1353 32 304
Fragments arranged by size:
1353 304 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 254 286 687 719 1207 1239
Size: 67 32 155 32 401 32 488 32
Cuts at: 1239 1595 1627 1689
Size: 356 32 62
Fragments arranged by size:
488 401 356 155 67 62 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 814 1689
Size: 814 875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 195 441 528 1555 1689
Size: 195 246 87 1027 134
Fragments arranged by size:
1027 246 195 134 87
ZraI GAC'GTC
Cuts at: 0 224 1464 1625 1689
Size: 224 1240 161 64
Fragments arranged by size:
1240 224 161 64
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbvCI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EciI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PpuMI PspOMI PstI PvuII
SanDI SfcI SfoI StuI StyI TaqII TatI TstI
Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AhdI AlfI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI
BciVI BclI BfrBI BmtI BplI BpuEI BsaI BsaBI
BsiWI BspEI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EarI
EcoICRI EcoRI EcoRV FalI FspI FspAI HindIII HpaI
KpnI MfeI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI
XmnI