Restriction map for
pF1KB3031
(Linear) (Six-base) MAPSORT of: KIBB3031.seq Check: 6965 from: 1 to: 1779
KIBB3031.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 489 1779
Size: 489 1290
AleI CACnn'nnGTG
Cuts at: 0 37 881 1376 1779
Size: 37 844 495 403
Fragments arranged by size:
844 495 403 37
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1394 1779
Size: 1394 385
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 1779
Size: 425 32 1322
Fragments arranged by size:
1322 425 32
AlwNI CAG_nnn'CTG
Cuts at: 0 528 1779
Size: 528 1251
ApaLI G'TGCA_C
Cuts at: 0 1324 1369 1779
Size: 1324 45 410
Fragments arranged by size:
1324 410 45
ApoI r'AATT_y
Cuts at: 0 208 748 937 1779
Size: 208 540 189 842
Fragments arranged by size:
842 540 208 189
AvaI C'yCGr_G
Cuts at: 0 1049 1779
Size: 1049 730
BanII G_rGCy'C
Cuts at: 0 1675 1779
Size: 1675 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 1727 1779
Size: 1727 52
BclI T'GATC_A
Cuts at: 0 855 1779
Size: 855 924
BglI GCCn_nnn'nGGC
Cuts at: 0 220 1779
Size: 220 1559
BglII A'GATC_T
Cuts at: 0 573 1779
Size: 573 1206
Bme1580I G_kGCm'C
Cuts at: 0 1286 1328 1373 1619 1779
Size: 1286 42 45 246 160
Fragments arranged by size:
1286 246 160 45 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 452 1336 1779
Size: 134 318 884 443
Fragments arranged by size:
884 443 318 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1779
Size: 619 1160
BsaI GGTCTCn'nnnn_
Cuts at: 0 706 1499 1779
Size: 706 793 280
Fragments arranged by size:
793 706 280
BsaBI GATnn'nnATC
Cuts at: 0 884 1779
Size: 884 895
BsaHI Gr'CG_yC
Cuts at: 0 1142 1779
Size: 1142 637
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 425 455 1779
Size: 425 30 1324
Fragments arranged by size:
1324 425 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 83 563 1333 1779
Size: 83 480 770 446
Fragments arranged by size:
770 480 446 83
BseYI C'CCAG_C
Cuts at: 0 1285 1779
Size: 1285 494
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 1727 1779
Size: 332 1395 52
Fragments arranged by size:
1395 332 52
BsiEI CG_ry'CG
Cuts at: 0 1364 1779
Size: 1364 415
BsiHKAI G_wGCw'C
Cuts at: 0 1328 1373 1779
Size: 1328 45 406
Fragments arranged by size:
1328 406 45
Bsp1286I G_dGCh'C
Cuts at: 0 1286 1328 1373 1619 1675 1779
Size: 1286 42 45 246 56 104
Fragments arranged by size:
1286 246 104 56 45 42
BspHI T'CATG_A
Cuts at: 0 1061 1179 1779
Size: 1061 118 600
Fragments arranged by size:
1061 600 118
BsrBI CCG'CTC
Cuts at: 0 1177 1779
Size: 1177 602
BsrDI GCAATG_nn'
Cuts at: 0 486 980 1779
Size: 486 494 799
Fragments arranged by size:
799 494 486
BsrFI r'CCGG_y
Cuts at: 0 1359 1779
Size: 1359 420
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1485 1660 1779
Size: 1485 175 119
Fragments arranged by size:
1485 175 119
BstYI r'GATC_y
Cuts at: 0 315 573 1779
Size: 315 258 1206
Fragments arranged by size:
1206 315 258
BtgI C'CryG_G
Cuts at: 0 1275 1779
Size: 1275 504
BtsI GCAGTG_nn'
Cuts at: 0 1371 1383 1779
Size: 1371 12 396
Fragments arranged by size:
1371 396 12
DraIII CAC_nnn'GTG
Cuts at: 0 1281 1705 1779
Size: 1281 424 74
Fragments arranged by size:
1281 424 74
EaeI y'GGCC_r
Cuts at: 0 1267 1389 1779
Size: 1267 122 390
Fragments arranged by size:
1267 390 122
EarI CTCTTCn'nnn_
Cuts at: 0 1586 1779
Size: 1586 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1041 1779
Size: 1041 738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 452 1336 1779
Size: 134 318 884 443
Fragments arranged by size:
884 443 318 134
EcoNI CCTnn'n_nnAGG
Cuts at: 0 327 1318 1779
Size: 327 991 461
Fragments arranged by size:
991 461 327
FspI TGC'GCA
Cuts at: 0 454 1779
Size: 454 1325
FspAI rTGC'GCAy
Cuts at: 0 454 1779
Size: 454 1325
HaeII r_GCGC'y
Cuts at: 0 1175 1779
Size: 1175 604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 475 504 507 536 1043 1075
Size: 307 32 136 29 3 29 507 32
Cuts at: 1075 1650 1682 1779
Size: 575 32 97
Fragments arranged by size:
575 507 307 136 97 32 32 32
29 29 3
HincII GTy'rAC
Cuts at: 0 62 1452 1779
Size: 62 1390 327
Fragments arranged by size:
1390 327 62
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1197 1717 1779
Size: 1197 520 62
Fragments arranged by size:
1197 520 62
MslI CAynn'nnrTG
Cuts at: 0 37 881 1376 1483 1779
Size: 37 844 495 107 296
Fragments arranged by size:
844 495 296 107 37
PasI CC'CwG_GG
Cuts at: 0 1294 1779
Size: 1294 485
PfoI T'CCnGG_A
Cuts at: 0 1313 1779
Size: 1313 466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 425 457 1779
Size: 425 32 1322
Fragments arranged by size:
1322 425 32
PsiI TTA'TAA
Cuts at: 0 590 1779
Size: 590 1189
PstI C_TGCA'G
Cuts at: 0 1378 1779
Size: 1378 401
SapI GCTCTTCn'nnn_
Cuts at: 0 1586 1779
Size: 1586 193
ScaI AGT'ACT
Cuts at: 0 626 1779
Size: 626 1153
SfcI C'TryA_G
Cuts at: 0 786 1374 1573 1779
Size: 786 588 199 206
Fragments arranged by size:
786 588 206 199
SmlI C'TyrA_G
Cuts at: 0 634 1779
Size: 634 1145
SspI AAT'ATT
Cuts at: 0 1632 1779
Size: 1632 147
StuI AGG'CCT
Cuts at: 0 96 1779
Size: 96 1683
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 38 169 1779
Size: 38 131 1610
Fragments arranged by size:
1610 131 38
TatI w'GTAC_w
Cuts at: 0 624 1687 1779
Size: 624 1063 92
Fragments arranged by size:
1063 624 92
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 427 1779
Size: 427 1352
XmnI GAAnn'nnTTC
Cuts at: 0 1771 1779
Size: 1771 8
Enzymes that do cut:
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AvaI
BanII BbsI BclI BglI BglII Bme1580I BpmI BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI BstXI BstYI
BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
FspI FspAI HaeII Hin4I HincII MmeI MslI PasI
PfoI PpiI PsiI PstI SapI ScaI SfcI SmlI
SspI StuI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BlpI
BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaWI BsiWI
BsmI BsmBI BspEI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpuMI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StyI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI