Restriction map for
pF1KB3021
(Linear) (Six-base) MAPSORT of: KIBB3021.seq Check: 2497 from: 1 to: 2784
KIBB3021.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 1242 2784
Size: 1242 1542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 1012 1172 2446 2609 2784
Size: 468 544 160 1274 163 175
Fragments arranged by size:
1274 544 468 175 163 160
AflIII A'CryG_T
Cuts at: 0 502 1858 2220 2784
Size: 502 1356 362 564
Fragments arranged by size:
1356 564 502 362
AlwNI CAG_nnn'CTG
Cuts at: 0 2289 2700 2784
Size: 2289 411 84
Fragments arranged by size:
2289 411 84
ApaLI G'TGCA_C
Cuts at: 0 779 2784
Size: 779 2005
ApoI r'AATT_y
Cuts at: 0 388 555 882 900 968 1167 1285 1413
Size: 388 167 327 18 68 199 118 128
Cuts at: 1413 1866 2158 2441 2686 2784
Size: 453 292 283 245 98
Fragments arranged by size:
453 388 327 292 283 245 199 167
128 118 98 68 18
AseI AT'TA_AT
Cuts at: 0 737 1153 2784
Size: 737 416 1631
Fragments arranged by size:
1631 737 416
BanI G'GyrC_C
Cuts at: 0 63 2784
Size: 63 2721
BbsI GAAGACnn'nnnn_
Cuts at: 0 2268 2784
Size: 2268 516
BbvCI CC'TCA_GC
Cuts at: 0 1899 2784
Size: 1899 885
BciVI GTATCCnnnnn_n'
Cuts at: 0 1261 2754 2784
Size: 1261 1493 30
Fragments arranged by size:
1493 1261 30
BclI T'GATC_A
Cuts at: 0 1179 1749 2228 2784
Size: 1179 570 479 556
Fragments arranged by size:
1179 570 556 479
BglII A'GATC_T
Cuts at: 0 987 1831 2784
Size: 987 844 953
Fragments arranged by size:
987 953 844
Bme1580I G_kGCm'C
Cuts at: 0 783 2784
Size: 783 2001
BmtI G_CTAG'C
Cuts at: 0 1972 2784
Size: 1972 812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1771 2420 2784
Size: 1103 668 649 364
Fragments arranged by size:
1103 668 649 364
Bpu10I CC'TnA_GC
Cuts at: 0 1899 2784
Size: 1899 885
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1492 2784
Size: 160 1332 1292
Fragments arranged by size:
1332 1292 160
BsaBI GATnn'nnATC
Cuts at: 0 1814 2784
Size: 1814 970
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 2254 2784
Size: 103 2151 530
Fragments arranged by size:
2151 530 103
BseYI C'CCAG_C
Cuts at: 0 529 2784
Size: 529 2255
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1203 2784
Size: 1203 1581
BsiEI CG_ry'CG
Cuts at: 0 29 2568 2784
Size: 29 2539 216
Fragments arranged by size:
2539 216 29
BsiHKAI G_wGCw'C
Cuts at: 0 783 2018 2576 2784
Size: 783 1235 558 208
Fragments arranged by size:
1235 783 558 208
BsmI GAATG_Cn'
Cuts at: 0 2735 2784
Size: 2735 49
Bsp1286I G_dGCh'C
Cuts at: 0 783 2018 2576 2784
Size: 783 1235 558 208
Fragments arranged by size:
1235 783 558 208
BspHI T'CATG_A
Cuts at: 0 1014 2081 2784
Size: 1014 1067 703
Fragments arranged by size:
1067 1014 703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 2784
Size: 131 2653
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2061 2784
Size: 2061 723
BstYI r'GATC_y
Cuts at: 0 987 1831 2187 2784
Size: 987 844 356 597
Fragments arranged by size:
987 844 597 356
BstZ17I GTA'TAC
Cuts at: 0 1243 2784
Size: 1243 1541
Bsu36I CC'TnA_GG
Cuts at: 0 86 2784
Size: 86 2698
BtgI C'CryG_G
Cuts at: 0 50 1543 2784
Size: 50 1493 1241
Fragments arranged by size:
1493 1241 50
BtsI GCAGTG_nn'
Cuts at: 0 1799 2784
Size: 1799 985
DraI TTT'AAA
Cuts at: 0 1166 1278 1568 2784
Size: 1166 112 290 1216
Fragments arranged by size:
1216 1166 290 112
DraIII CAC_nnn'GTG
Cuts at: 0 1996 2784
Size: 1996 788
EaeI y'GGCC_r
Cuts at: 0 26 1484 2784
Size: 26 1458 1300
Fragments arranged by size:
1458 1300 26
EagI C'GGCC_G
Cuts at: 0 26 2784
Size: 26 2758
EarI CTCTTCn'nnn_
Cuts at: 0 487 1031 1085 2231 2422 2771 2784
Size: 487 544 54 1146 191 349 13
Fragments arranged by size:
1146 544 487 349 191 54 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 1012 1103 1172 1771 2420 2446 2609
Size: 468 544 91 69 599 649 26 163
Cuts at: 2609 2784
Size: 175
Fragments arranged by size:
649 599 544 468 175 163 91 69
26
EcoO109I rG'GnC_Cy
Cuts at: 0 93 120 2324 2784
Size: 93 27 2204 460
Fragments arranged by size:
2204 460 93 27
EcoRI G'AATT_C
Cuts at: 0 900 2784
Size: 900 1884
EcoRV GAT'ATC
Cuts at: 0 542 2784
Size: 542 2242
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 186 218 322 354 2393 2425 2474 2506
Size: 186 32 104 32 2039 32 49 32
Cuts at: 2506 2703 2735 2784
Size: 197 32 49
Fragments arranged by size:
2039 197 186 104 49 49 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 227 324 2784
Size: 227 97 2460
Fragments arranged by size:
2460 227 97
HpaI GTT'AAC
Cuts at: 0 227 2784
Size: 227 2557
MfeI C'AATT_G
Cuts at: 0 2501 2784
Size: 2501 283
MluI A'CGCG_T
Cuts at: 0 1858 2784
Size: 1858 926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1170 2784
Size: 1170 1614
MscI TGG'CCA
Cuts at: 0 1486 2784
Size: 1486 1298
MspA1I CmG'CkG
Cuts at: 0 43 52 110 724 2784
Size: 43 9 58 614 2060
Fragments arranged by size:
2060 614 58 43 9
NcoI C'CATG_G
Cuts at: 0 1543 2784
Size: 1543 1241
NdeI CA'TA_TG
Cuts at: 0 1448 2131 2784
Size: 1448 683 653
Fragments arranged by size:
1448 683 653
NheI G'CTAG_C
Cuts at: 0 1968 2784
Size: 1968 816
NspI r_CATG'y
Cuts at: 0 506 2784
Size: 506 2278
PciI A'CATG_T
Cuts at: 0 502 2784
Size: 502 2282
PfoI T'CCnGG_A
Cuts at: 0 2397 2784
Size: 2397 387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 2784
Size: 325 32 2427
Fragments arranged by size:
2427 325 32
PpuMI rG'GwC_Cy
Cuts at: 0 93 120 2784
Size: 93 27 2664
Fragments arranged by size:
2664 93 27
PsiI TTA'TAA
Cuts at: 0 1952 2784
Size: 1952 832
PstI C_TGCA'G
Cuts at: 0 1806 2066 2784
Size: 1806 260 718
Fragments arranged by size:
1806 718 260
PvuII CAG'CTG
Cuts at: 0 724 2784
Size: 724 2060
SacII CC_GC'GG
Cuts at: 0 53 2784
Size: 53 2731
SfcI C'TryA_G
Cuts at: 0 358 1040 1802 2062 2784
Size: 358 682 762 260 722
Fragments arranged by size:
762 722 682 358 260
SmlI C'TyrA_G
Cuts at: 0 139 1471 2784
Size: 139 1332 1313
Fragments arranged by size:
1332 1313 139
SspI AAT'ATT
Cuts at: 0 2301 2784
Size: 2301 483
StyI C'CwwG_G
Cuts at: 0 1543 2784
Size: 1543 1241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 771 1748 2033 2262 2784
Size: 771 977 285 229 522
Fragments arranged by size:
977 771 522 285 229
TatI w'GTAC_w
Cuts at: 0 1501 2784
Size: 1501 1283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1212 1244 2784
Size: 1212 32 1540
Fragments arranged by size:
1540 1212 32
XbaI T'CTAG_A
Cuts at: 0 990 2784
Size: 990 1794
XmnI GAAnn'nnTTC
Cuts at: 0 2003 2784
Size: 2003 781
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaLI ApoI AseI BanI
BbsI BbvCI BciVI BclI BglII Bme1580I BmtI BpmI
Bpu10I BpuEI BsaBI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BstAPI BstYI BstZ17I Bsu36I
BtgI BtsI DraI DraIII EaeI EagI EarI Eco57MI
EcoO109I EcoRI EcoRV Hin4I HincII HpaI MfeI MluI
MmeI MscI MspA1I NcoI NdeI NheI NspI PciI
PfoI PpiI PpuMI PsiI PstI PvuII SacII SfcI
SmlI SspI StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BcgI BfrBI BglI BlpI
BmgBI BmrI BplI BsaI BsaAI BsaHI BsaWI BsaXI
BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BtgZI ClaI CspCI DrdI
EciI EcoICRI EcoNI FalI FseI FspI FspAI HaeII
HindIII KasI KpnI MslI NaeI NarI NgoMIV NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XcmI XhoI XmaI ZraI