Restriction map for
pF1KB3016
(Linear) (Six-base) MAPSORT of: KIBB3016.seq Check: 7486 from: 1 to: 2337
KIBB3016.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AatII G_ACGT'C
Cuts at: 0 2219 2337
Size: 2219 118
AccI GT'mk_AC
Cuts at: 0 2036 2337
Size: 2036 301
AclI AA'CG_TT
Cuts at: 0 1915 2337
Size: 1915 422
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1187 2337
Size: 377 810 1150
Fragments arranged by size:
1150 810 377
AflIII A'CryG_T
Cuts at: 0 1486 2337
Size: 1486 851
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 1931 1963 2337
Size: 383 32 1516 32 374
Fragments arranged by size:
1516 383 374 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 892 2337
Size: 892 1445
ApoI r'AATT_y
Cuts at: 0 496 718 2101 2337
Size: 496 222 1383 236
Fragments arranged by size:
1383 496 236 222
BamHI G'GATC_C
Cuts at: 0 379 1356 2337
Size: 379 977 981
Fragments arranged by size:
981 977 379
BanI G'GyrC_C
Cuts at: 0 30 1715 2337
Size: 30 1685 622
Fragments arranged by size:
1685 622 30
BanII G_rGCy'C
Cuts at: 0 1704 2274 2337
Size: 1704 570 63
Fragments arranged by size:
1704 570 63
BbeI G_GCGC'C
Cuts at: 0 34 2337
Size: 34 2303
BbsI GAAGACnn'nnnn_
Cuts at: 0 1560 2337
Size: 1560 777
BbvCI CC'TCA_GC
Cuts at: 0 1965 2337
Size: 1965 372
BciVI GTATCCnnnnn_n'
Cuts at: 0 385 2337
Size: 385 1952
BglI GCCn_nnn'nGGC
Cuts at: 0 39 1190 2337
Size: 39 1151 1147
Fragments arranged by size:
1151 1147 39
BglII A'GATC_T
Cuts at: 0 1524 1900 2337
Size: 1524 376 437
Fragments arranged by size:
1524 437 376
BmgBI CAC'GTC
Cuts at: 0 128 2137 2337
Size: 128 2009 200
Fragments arranged by size:
2009 200 128
BmrI ACTGGGnnnn_n'
Cuts at: 0 1939 2337
Size: 1939 398
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 102 2337
Size: 102 2235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 836 1159 1661 1688 2337
Size: 55 781 323 502 27 649
Fragments arranged by size:
781 649 502 323 55 27
Bpu10I CC'TnA_GC
Cuts at: 0 1965 2337
Size: 1965 372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2302 2337
Size: 2302 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 59 473 1819 2337
Size: 59 414 1346 518
Fragments arranged by size:
1346 518 414 59
BsaHI Gr'CG_yC
Cuts at: 0 31 2216 2337
Size: 31 2185 121
Fragments arranged by size:
2185 121 31
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 996 2337
Size: 61 935 1341
Fragments arranged by size:
1341 935 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1511 2337
Size: 1511 826
BsiHKAI G_wGCw'C
Cuts at: 0 1704 2337
Size: 1704 633
BsmI GAATG_Cn'
Cuts at: 0 658 2337
Size: 658 1679
Bsp1286I G_dGCh'C
Cuts at: 0 1704 2274 2337
Size: 1704 570 63
Fragments arranged by size:
1704 570 63
BspHI T'CATG_A
Cuts at: 0 1974 2010 2337
Size: 1974 36 327
Fragments arranged by size:
1974 327 36
BsrBI CCG'CTC
Cuts at: 0 57 81 2337
Size: 57 24 2256
Fragments arranged by size:
2256 57 24
BsrDI GCAATG_nn'
Cuts at: 0 1553 2337
Size: 1553 784
BssSI C'ACGA_G
Cuts at: 0 740 2337
Size: 740 1597
BstEII G'GTnAC_C
Cuts at: 0 2022 2337
Size: 2022 315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1712 2337
Size: 1712 625
BstYI r'GATC_y
Cuts at: 0 379 1220 1356 1524 1900 2337
Size: 379 841 136 168 376 437
Fragments arranged by size:
841 437 379 376 168 136
BtgI C'CryG_G
Cuts at: 0 788 2337
Size: 788 1549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 84 2337
Size: 84 2253
BtsI GCAGTG_nn'
Cuts at: 0 2130 2337
Size: 2130 207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1677 1712 2337
Size: 1677 35 625
Fragments arranged by size:
1677 625 35
DraI TTT'AAA
Cuts at: 0 611 2337
Size: 611 1726
EaeI y'GGCC_r
Cuts at: 0 840 1182 1428 2337
Size: 840 342 246 909
Fragments arranged by size:
909 840 342 246
EarI CTCTTCn'nnn_
Cuts at: 0 71 107 2337
Size: 71 36 2230
Fragments arranged by size:
2230 71 36
EcoICRI GAG'CTC
Cuts at: 0 1702 2337
Size: 1702 635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 377 836 1159 1187 1661 1688 2337
Size: 55 322 459 323 28 474 27 649
Fragments arranged by size:
649 474 459 323 322 55 28 27
EcoO109I rG'GnC_Cy
Cuts at: 0 1837 2300 2337
Size: 1837 463 37
Fragments arranged by size:
1837 463 37
EcoRV GAT'ATC
Cuts at: 0 549 795 2337
Size: 549 246 1542
Fragments arranged by size:
1542 549 246
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1792 2337
Size: 1792 545
HaeII r_GCGC'y
Cuts at: 0 34 2337
Size: 34 2303
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 957 989 1772 1804 2191 2223 2236 2268
Size: 957 32 783 32 387 32 13 32
Cuts at: 2268 2337
Size: 69
Fragments arranged by size:
957 783 387 69 32 32 32 32
13
HindIII A'AGCT_T
Cuts at: 0 1795 2337
Size: 1795 542
KasI G'GCGC_C
Cuts at: 0 30 2337
Size: 30 2307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1109 2337
Size: 175 934 1228
Fragments arranged by size:
1228 934 175
MscI TGG'CCA
Cuts at: 0 842 1430 2337
Size: 842 588 907
Fragments arranged by size:
907 842 588
MslI CAynn'nnrTG
Cuts at: 0 803 2337
Size: 803 1534
NarI GG'CG_CC
Cuts at: 0 31 2337
Size: 31 2306
NcoI C'CATG_G
Cuts at: 0 788 2337
Size: 788 1549
NspI r_CATG'y
Cuts at: 0 1490 2337
Size: 1490 847
PciI A'CATG_T
Cuts at: 0 1486 2337
Size: 1486 851
PflMI CCAn_nnn'nTGG
Cuts at: 0 1006 2337
Size: 1006 1331
PfoI T'CCnGG_A
Cuts at: 0 71 1136 2337
Size: 71 1065 1201
Fragments arranged by size:
1201 1065 71
PpuMI rG'GwC_Cy
Cuts at: 0 2300 2337
Size: 2300 37
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 525 557 2337
Size: 525 32 1780
Fragments arranged by size:
1780 525 32
SacI G_AGCT'C
Cuts at: 0 1704 2337
Size: 1704 633
SapI GCTCTTCn'nnn_
Cuts at: 0 71 2337
Size: 71 2266
ScaI AGT'ACT
Cuts at: 0 1853 2337
Size: 1853 484
SfcI C'TryA_G
Cuts at: 0 542 1224 1298 2337
Size: 542 682 74 1039
Fragments arranged by size:
1039 682 542 74
SfoI GGC'GCC
Cuts at: 0 32 2337
Size: 32 2305
SmlI C'TyrA_G
Cuts at: 0 2281 2337
Size: 2281 56
SpeI A'CTAG_T
Cuts at: 0 1285 2337
Size: 1285 1052
SspI AAT'ATT
Cuts at: 0 680 944 1038 2337
Size: 680 264 94 1299
Fragments arranged by size:
1299 680 264 94
StyI C'CwwG_G
Cuts at: 0 34 788 2337
Size: 34 754 1549
Fragments arranged by size:
1549 754 34
TatI w'GTAC_w
Cuts at: 0 1851 2337
Size: 1851 486
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1125 2337
Size: 1125 1212
ZraI GAC'GTC
Cuts at: 0 2217 2337
Size: 2217 120
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AloI AlwNI ApoI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI
BglII BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI
BsrDI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I HindIII KasI MmeI MscI MslI
NarI NcoI NspI PciI PflMI PfoI PpuMI PsrI
SacI SapI ScaI SfcI SfoI SmlI SpeI SspI
StyI TatI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AgeI AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BcgI BclI BfrBI BlpI Bme1580I BmtI BsaAI BsaBI
BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspMI
BsrFI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI
DraIII DrdI EagI EciI EcoNI EcoRI FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI