Restriction map for
pF1KB3008
(Linear) (Six-base) MAPSORT of: KIBB3008.seq Check: 110 from: 1 to: 2466
KIBB3008.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 0 624 2466
Size: 624 1842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 2324 2466
Size: 1054 1270 142
Fragments arranged by size:
1270 1054 142
AflIII A'CryG_T
Cuts at: 0 1963 2466
Size: 1963 503
AhdI GACnn_n'nnGTC
Cuts at: 0 2099 2466
Size: 2099 367
AleI CACnn'nnGTG
Cuts at: 0 453 676 2466
Size: 453 223 1790
Fragments arranged by size:
1790 453 223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 471 2020 2466
Size: 471 1549 446
Fragments arranged by size:
1549 471 446
AlwNI CAG_nnn'CTG
Cuts at: 0 1375 1777 1894 1930 2466
Size: 1375 402 117 36 536
Fragments arranged by size:
1375 536 402 117 36
ApaLI G'TGCA_C
Cuts at: 0 2255 2466
Size: 2255 211
ApoI r'AATT_y
Cuts at: 0 378 841 979 2122 2466
Size: 378 463 138 1143 344
Fragments arranged by size:
1143 463 378 344 138
BamHI G'GATC_C
Cuts at: 0 1501 2466
Size: 1501 965
BanII G_rGCy'C
Cuts at: 0 645 1316 1649 2466
Size: 645 671 333 817
Fragments arranged by size:
817 671 645 333
BbsI GAAGACnn'nnnn_
Cuts at: 0 2197 2400 2466
Size: 2197 203 66
Fragments arranged by size:
2197 203 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1453 1487 2466
Size: 1453 34 979
Fragments arranged by size:
1453 979 34
BclI T'GATC_A
Cuts at: 0 242 2466
Size: 242 2224
BfrBI ATG'CAT
Cuts at: 0 420 2466
Size: 420 2046
BglII A'GATC_T
Cuts at: 0 483 1353 2466
Size: 483 870 1113
Fragments arranged by size:
1113 870 483
Bme1580I G_kGCm'C
Cuts at: 0 2259 2466
Size: 2259 207
BmgBI CAC'GTC
Cuts at: 0 1966 2195 2466
Size: 1966 229 271
Fragments arranged by size:
1966 271 229
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 2466
Size: 20 2446
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2129 2466
Size: 2129 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1754 1907 1965 2466
Size: 394 1360 153 58 501
Fragments arranged by size:
1360 501 394 153 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 2466
Size: 290 2176
BsaI GGTCTCn'nnnn_
Cuts at: 0 1758 2466
Size: 1758 708
BsaBI GATnn'nnATC
Cuts at: 0 488 1358 2466
Size: 488 870 1108
Fragments arranged by size:
1108 870 488
BsaHI Gr'CG_yC
Cuts at: 0 590 2466
Size: 590 1876
BsaWI w'CCGG_w
Cuts at: 0 2034 2466
Size: 2034 432
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 926 956 1875 1905 1963 1993 2466
Size: 926 30 919 30 58 30 473
Fragments arranged by size:
926 919 473 58 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1868 2466
Size: 1868 598
BseYI C'CCAG_C
Cuts at: 0 1307 1950 2466
Size: 1307 643 516
Fragments arranged by size:
1307 643 516
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1126 2466
Size: 1126 1340
BsiHKAI G_wGCw'C
Cuts at: 0 645 1649 2259 2466
Size: 645 1004 610 207
Fragments arranged by size:
1004 645 610 207
BsmI GAATG_Cn'
Cuts at: 0 420 1434 2466
Size: 420 1014 1032
Fragments arranged by size:
1032 1014 420
Bsp1286I G_dGCh'C
Cuts at: 0 645 1316 1649 2259 2466
Size: 645 671 333 610 207
Fragments arranged by size:
671 645 610 333 207
BspHI T'CATG_A
Cuts at: 0 756 2466
Size: 756 1710
BsrBI CCG'CTC
Cuts at: 0 2113 2466
Size: 2113 353
BsrDI GCAATG_nn'
Cuts at: 0 1308 1702 2466
Size: 1308 394 764
Fragments arranged by size:
1308 764 394
BsrGI T'GTAC_A
Cuts at: 0 2334 2466
Size: 2334 132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2145 2466
Size: 2145 321
BstBI TT'CG_AA
Cuts at: 0 1710 2466
Size: 1710 756
BstYI r'GATC_y
Cuts at: 0 483 1353 1501 2466
Size: 483 870 148 965
Fragments arranged by size:
965 870 483 148
BstZ17I GTA'TAC
Cuts at: 0 625 2466
Size: 625 1841
Bsu36I CC'TnA_GG
Cuts at: 0 595 2466
Size: 595 1871
BtgI C'CryG_G
Cuts at: 0 2134 2466
Size: 2134 332
BtsI GCAGTG_nn'
Cuts at: 0 177 402 2367 2466
Size: 177 225 1965 99
Fragments arranged by size:
1965 225 177 99
DraI TTT'AAA
Cuts at: 0 389 2466
Size: 389 2077
EaeI y'GGCC_r
Cuts at: 0 1025 1258 2466
Size: 1025 233 1208
Fragments arranged by size:
1208 1025 233
EarI CTCTTCn'nnn_
Cuts at: 0 499 571 2466
Size: 499 72 1895
Fragments arranged by size:
1895 499 72
EcoICRI GAG'CTC
Cuts at: 0 643 1647 2466
Size: 643 1004 819
Fragments arranged by size:
1004 819 643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1054 1754 1907 1965 2324 2466
Size: 394 660 700 153 58 359 142
Fragments arranged by size:
700 660 394 359 153 142 58
EcoO109I rG'GnC_Cy
Cuts at: 0 126 1120 1947 2466
Size: 126 994 827 519
Fragments arranged by size:
994 827 519 126
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1638 2466
Size: 1638 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 2085 2117 2466
Size: 343 32 1710 32 349
Fragments arranged by size:
1710 349 343 32 32
MfeI C'AATT_G
Cuts at: 0 1169 2466
Size: 1169 1297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1906 2083 2466
Size: 1906 177 383
Fragments arranged by size:
1906 383 177
MscI TGG'CCA
Cuts at: 0 1027 1260 2466
Size: 1027 233 1206
Fragments arranged by size:
1206 1027 233
MslI CAynn'nnrTG
Cuts at: 0 453 676 1439 2466
Size: 453 223 763 1027
Fragments arranged by size:
1027 763 453 223
MspA1I CmG'CkG
Cuts at: 0 1212 1852 2466
Size: 1212 640 614
Fragments arranged by size:
1212 640 614
NdeI CA'TA_TG
Cuts at: 0 1284 2466
Size: 1284 1182
NsiI A_TGCA'T
Cuts at: 0 422 2466
Size: 422 2044
PasI CC'CwG_GG
Cuts at: 0 661 2466
Size: 661 1805
PflMI CCAn_nnn'nTGG
Cuts at: 0 828 1465 1777 2466
Size: 828 637 312 689
Fragments arranged by size:
828 689 637 312
PfoI T'CCnGG_A
Cuts at: 0 221 2466
Size: 221 2245
PpuMI rG'GwC_Cy
Cuts at: 0 1120 1947 2466
Size: 1120 827 519
Fragments arranged by size:
1120 827 519
PstI C_TGCA'G
Cuts at: 0 1065 2466
Size: 1065 1401
PvuII CAG'CTG
Cuts at: 0 1212 1852 2466
Size: 1212 640 614
Fragments arranged by size:
1212 640 614
SacI G_AGCT'C
Cuts at: 0 645 1649 2466
Size: 645 1004 817
Fragments arranged by size:
1004 817 645
ScaI AGT'ACT
Cuts at: 0 1142 2466
Size: 1142 1324
SfcI C'TryA_G
Cuts at: 0 1061 2319 2466
Size: 1061 1258 147
Fragments arranged by size:
1258 1061 147
SmlI C'TyrA_G
Cuts at: 0 305 2466
Size: 305 2161
StyI C'CwwG_G
Cuts at: 0 287 726 745 1515 2466
Size: 287 439 19 770 951
Fragments arranged by size:
951 770 439 287 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 651 688 2057 2466
Size: 651 37 1369 409
Fragments arranged by size:
1369 651 409 37
TatI w'GTAC_w
Cuts at: 0 1140 2334 2466
Size: 1140 1194 132
Fragments arranged by size:
1194 1140 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1626 1658 2466
Size: 1626 32 808
Fragments arranged by size:
1626 808 32
Tth111I GACn'n_nGTC
Cuts at: 0 163 2466
Size: 163 2303
XmnI GAAnn'nnTTC
Cuts at: 0 2122 2466
Size: 2122 344
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaLI
ApoI BamHI BanII BbsI BcgI BclI BfrBI BglII
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoO109I FalI Hin4I MfeI MmeI MscI MslI
MspA1I NdeI NsiI PasI PflMI PfoI PpuMI PstI
PvuII SacI ScaI SfcI SmlI StyI TaqII TatI
TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
ApaI AscI AseI AsiSI AvaI AvrII BaeI BanI
BbeI BbvCI BciVI BglI BlpI BmtI Bpu10I BsaAI
BsiEI BsiWI BsmBI BspEI BspMI BsrFI BssHII BssSI
BstEII BstXI BtgZI ClaI CspCI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI
ZraI