Restriction map for
pF1KSDB2508
(Linear) (Six-base) MAPSORT of: KIBB2508.seq Check: 1009 from: 1 to: 714
KIBB2508.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 714
Size: 388 326
AfeI AGC'GCT
Cuts at: 0 63 714
Size: 63 651
AflIII A'CryG_T
Cuts at: 0 209 714
Size: 209 505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 82 714
Size: 82 632
AlwNI CAG_nnn'CTG
Cuts at: 0 195 547 621 714
Size: 195 352 74 93
Fragments arranged by size:
352 195 93 74
ApaLI G'TGCA_C
Cuts at: 0 132 223 714
Size: 132 91 491
Fragments arranged by size:
491 132 91
AvaI C'yCGr_G
Cuts at: 0 51 714
Size: 51 663
BamHI G'GATC_C
Cuts at: 0 496 714
Size: 496 218
BanI G'GyrC_C
Cuts at: 0 35 109 175 714
Size: 35 74 66 539
Fragments arranged by size:
539 74 66 35
BanII G_rGCy'C
Cuts at: 0 52 152 656 714
Size: 52 100 504 58
Fragments arranged by size:
504 100 58 52
BbeI G_GCGC'C
Cuts at: 0 39 113 714
Size: 39 74 601
Fragments arranged by size:
601 74 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 123 157 714
Size: 123 34 557
Fragments arranged by size:
557 123 34
BglI GCCn_nnn'nGGC
Cuts at: 0 33 56 714
Size: 33 23 658
Fragments arranged by size:
658 33 23
Bme1580I G_kGCm'C
Cuts at: 0 136 227 714
Size: 136 91 487
Fragments arranged by size:
487 136 91
BmgBI CAC'GTC
Cuts at: 0 210 714
Size: 210 504
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 648 714
Size: 648 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 714
Size: 643 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 207 714
Size: 207 507
BsaHI Gr'CG_yC
Cuts at: 0 36 110 714
Size: 36 74 604
Fragments arranged by size:
604 74 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 60 625 714
Size: 60 565 89
Fragments arranged by size:
565 89 60
BseYI C'CCAG_C
Cuts at: 0 195 467 714
Size: 195 272 247
Fragments arranged by size:
272 247 195
BsiHKAI G_wGCw'C
Cuts at: 0 136 227 714
Size: 136 91 487
Fragments arranged by size:
487 136 91
Bsp1286I G_dGCh'C
Cuts at: 0 52 136 152 227 656 714
Size: 52 84 16 75 429 58
Fragments arranged by size:
429 84 75 58 52 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 242 714
Size: 242 472
BsrFI r'CCGG_y
Cuts at: 0 38 714
Size: 38 676
BstYI r'GATC_y
Cuts at: 0 496 714
Size: 496 218
BtgI C'CryG_G
Cuts at: 0 168 492 569 714
Size: 168 324 77 145
Fragments arranged by size:
324 168 145 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 270 714
Size: 270 444
BtsI GCAGTG_nn'
Cuts at: 0 134 421 714
Size: 134 287 293
Fragments arranged by size:
293 287 134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 411 446 714
Size: 411 35 268
Fragments arranged by size:
411 268 35
EaeI y'GGCC_r
Cuts at: 0 566 714
Size: 566 148
EarI CTCTTCn'nnn_
Cuts at: 0 602 638 714
Size: 602 36 76
Fragments arranged by size:
602 76 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 57 679 714
Size: 57 622 35
Fragments arranged by size:
622 57 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 643 714
Size: 388 255 71
Fragments arranged by size:
388 255 71
HaeII r_GCGC'y
Cuts at: 0 39 65 113 130 622 714
Size: 39 26 48 17 492 92
Fragments arranged by size:
492 92 48 39 26 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 363 395 488 520 545 577 714
Size: 363 32 93 32 25 32 137
Fragments arranged by size:
363 137 93 32 32 32 25
KasI G'GCGC_C
Cuts at: 0 35 109 714
Size: 35 74 605
Fragments arranged by size:
605 74 35
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 714
Size: 230 484
MscI TGG'CCA
Cuts at: 0 568 714
Size: 568 146
NaeI GCC'GGC
Cuts at: 0 40 714
Size: 40 674
NarI GG'CG_CC
Cuts at: 0 36 110 714
Size: 36 74 604
Fragments arranged by size:
604 74 36
NcoI C'CATG_G
Cuts at: 0 492 714
Size: 492 222
NgoMIV G'CCGG_C
Cuts at: 0 38 714
Size: 38 676
PflMI CCAn_nnn'nTGG
Cuts at: 0 195 714
Size: 195 519
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 640 672 714
Size: 640 32 42
Fragments arranged by size:
640 42 32
PstI C_TGCA'G
Cuts at: 0 416 714
Size: 416 298
SexAI A'CCwGG_T
Cuts at: 0 533 714
Size: 533 181
SfcI C'TryA_G
Cuts at: 0 412 714
Size: 412 302
SfoI GGC'GCC
Cuts at: 0 37 111 714
Size: 37 74 603
Fragments arranged by size:
603 74 37
SgrAI Cr'CCGG_yG
Cuts at: 0 38 714
Size: 38 676
SmaI CCC'GGG
Cuts at: 0 53 714
Size: 53 661
StyI C'CwwG_G
Cuts at: 0 492 714
Size: 492 222
XmaI C'CCGG_G
Cuts at: 0 51 714
Size: 51 663
XmnI GAAnn'nnTTC
Cuts at: 0 244 714
Size: 244 470
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI ApaLI AvaI BamHI
BanI BanII BbeI BcgI BglI Bme1580I BmgBI BplI
BpmI BsaI BsaHI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrFI BstYI BtgI BtgZI BtsI CspCI EaeI EarI
EciI Eco57MI HaeII Hin4I KasI MmeI MscI NaeI
NarI NcoI NgoMIV PflMI PsrI PstI SexAI SfcI
SfoI SgrAI SmaI StyI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MslI MspA1I
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SgfI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI ZraI