Restriction map for
pF1KB2260
(Linear) (Six-base) MAPSORT of: KIBB2260.seq Check: 473 from: 1 to: 2628
KIBB2260.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 252 2478 2628
Size: 252 2226 150
Fragments arranged by size:
2226 252 150
AccI GT'mk_AC
Cuts at: 0 1775 2628
Size: 1775 853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1059 1167 2628
Size: 475 584 108 1461
Fragments arranged by size:
1461 584 475 108
AflIII A'CryG_T
Cuts at: 0 2363 2628
Size: 2363 265
AleI CACnn'nnGTG
Cuts at: 0 1766 2628
Size: 1766 862
AlwNI CAG_nnn'CTG
Cuts at: 0 155 738 2628
Size: 155 583 1890
Fragments arranged by size:
1890 583 155
ApaI G_GGCC'C
Cuts at: 0 470 1064 1688 2067 2096 2415 2628
Size: 470 594 624 379 29 319 213
Fragments arranged by size:
624 594 470 379 319 213 29
ApaLI G'TGCA_C
Cuts at: 0 1759 2628
Size: 1759 869
ApoI r'AATT_y
Cuts at: 0 2217 2628
Size: 2217 411
AvaI C'yCGr_G
Cuts at: 0 368 503 992 2628
Size: 368 135 489 1636
Fragments arranged by size:
1636 489 368 135
AvrII C'CTAG_G
Cuts at: 0 963 2628
Size: 963 1665
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1432 1465 2628
Size: 1432 33 1163
Fragments arranged by size:
1432 1163 33
BanI G'GyrC_C
Cuts at: 0 218 301 1132 1549 2628
Size: 218 83 831 417 1079
Fragments arranged by size:
1079 831 417 218 83
BanII G_rGCy'C
Cuts at: 0 470 545 885 950 993 1064 1205 1243
Size: 470 75 340 65 43 71 141 38
Cuts at: 1243 1460 1688 2067 2096 2415 2628
Size: 217 228 379 29 319 213
Fragments arranged by size:
470 379 340 319 228 217 213 141
75 71 65 43 38 29
BbeI G_GCGC'C
Cuts at: 0 1553 2628
Size: 1553 1075
BbvCI CC'TCA_GC
Cuts at: 0 829 1339 2628
Size: 829 510 1289
Fragments arranged by size:
1289 829 510
BglI GCCn_nnn'nGGC
Cuts at: 0 841 2062 2424 2522 2628
Size: 841 1221 362 98 106
Fragments arranged by size:
1221 841 362 106 98
BglII A'GATC_T
Cuts at: 0 1788 1853 2628
Size: 1788 65 775
Fragments arranged by size:
1788 775 65
Bme1580I G_kGCm'C
Cuts at: 0 223 304 470 1010 1064 1688 1763 2034
Size: 223 81 166 540 54 624 75 271
Cuts at: 2034 2067 2096 2415 2628
Size: 33 29 319 213
Fragments arranged by size:
624 540 319 271 223 213 166 81
75 54 33 29
BmgBI CAC'GTC
Cuts at: 0 2364 2628
Size: 2364 264
BmrI ACTGGGnnnn_n'
Cuts at: 0 1135 2628
Size: 1135 1493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 438 2628
Size: 438 2190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 774 1219 2062 2571 2628
Size: 579 195 445 843 509 57
Fragments arranged by size:
843 579 509 445 195 57
Bpu10I CC'TnA_GC
Cuts at: 0 829 1339 2628
Size: 829 510 1289
Fragments arranged by size:
1289 829 510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1769 1909 2628
Size: 1769 140 719
Fragments arranged by size:
1769 719 140
BsaHI Gr'CG_yC
Cuts at: 0 1550 1625 2628
Size: 1550 75 1003
Fragments arranged by size:
1550 1003 75
BsaWI w'CCGG_w
Cuts at: 0 116 2628
Size: 116 2512
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 268 772 796 802 826 832 2628
Size: 238 30 504 24 6 24 6 1796
Fragments arranged by size:
1796 504 238 30 24 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 265 1327 1384 2628
Size: 262 3 1062 57 1244
Fragments arranged by size:
1244 1062 262 57 3
BseYI C'CCAG_C
Cuts at: 0 404 876 1950 2266 2414 2585 2628
Size: 404 472 1074 316 148 171 43
Fragments arranged by size:
1074 472 404 316 171 148 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1306 1640 1984 2305 2628
Size: 265 1041 334 344 321 323
Fragments arranged by size:
1041 344 334 323 321 265
BsiEI CG_ry'CG
Cuts at: 0 1020 1247 2506 2628
Size: 1020 227 1259 122
Fragments arranged by size:
1259 1020 227 122
BsiHKAI G_wGCw'C
Cuts at: 0 545 1763 2628
Size: 545 1218 865
Fragments arranged by size:
1218 865 545
BsmI GAATG_Cn'
Cuts at: 0 1884 2628
Size: 1884 744
Bsp1286I G_dGCh'C
Cuts at: 0 223 304 470 545 885 950 993 1010
Size: 223 81 166 75 340 65 43 17
Cuts at: 1010 1064 1205 1243 1460 1688 1763 2034 2067
Size: 54 141 38 217 228 75 271 33
Cuts at: 2067 2096 2415 2628
Size: 29 319 213
Fragments arranged by size:
340 319 271 228 223 217 213 166
141 81 75 75 65 54 43 38
33 29 17
BspHI T'CATG_A
Cuts at: 0 518 2628
Size: 518 2110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 252 840 2433 2478 2628
Size: 176 76 588 1593 45 150
Fragments arranged by size:
1593 588 176 150 76 45
BsrDI GCAATG_nn'
Cuts at: 0 435 1298 2628
Size: 435 863 1330
Fragments arranged by size:
1330 863 435
BsrFI r'CCGG_y
Cuts at: 0 353 824 2483 2628
Size: 353 471 1659 145
Fragments arranged by size:
1659 471 353 145
BsrGI T'GTAC_A
Cuts at: 0 2175 2628
Size: 2175 453
BssSI C'ACGA_G
Cuts at: 0 1466 2628
Size: 1466 1162
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2611 2628
Size: 2611 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1151 2422 2628
Size: 1151 1271 206
Fragments arranged by size:
1271 1151 206
BstYI r'GATC_y
Cuts at: 0 267 1788 1853 2628
Size: 267 1521 65 775
Fragments arranged by size:
1521 775 267 65
Bsu36I CC'TnA_GG
Cuts at: 0 440 2628
Size: 440 2188
BtgI C'CryG_G
Cuts at: 0 473 656 929 1220 2628
Size: 473 183 273 291 1408
Fragments arranged by size:
1408 473 291 273 183
BtsI GCAGTG_nn'
Cuts at: 0 167 204 1045 1657 2303 2628
Size: 167 37 841 612 646 325
Fragments arranged by size:
841 646 612 325 167 37
DraIII CAC_nnn'GTG
Cuts at: 0 288 2628
Size: 288 2340
DrdI GACnn_nn'nnGTC
Cuts at: 0 2443 2628
Size: 2443 185
EaeI y'GGCC_r
Cuts at: 0 748 1017 1156 1223 1271 1861 2159 2189
Size: 748 269 139 67 48 590 298 30
Cuts at: 2189 2503 2628
Size: 314 125
Fragments arranged by size:
748 590 314 298 269 139 125 67
48 30
EagI C'GGCC_G
Cuts at: 0 1017 2503 2628
Size: 1017 1486 125
Fragments arranged by size:
1486 1017 125
EarI CTCTTCn'nnn_
Cuts at: 0 721 1078 1361 2628
Size: 721 357 283 1267
Fragments arranged by size:
1267 721 357 283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 677 1466 2628
Size: 677 789 1162
Fragments arranged by size:
1162 789 677
EcoICRI GAG'CTC
Cuts at: 0 543 2628
Size: 543 2085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 579 774 1059 1167 1219 2062 2571
Size: 475 104 195 285 108 52 843 509
Cuts at: 2571 2628
Size: 57
Fragments arranged by size:
843 509 475 285 195 108 104 57
52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 1342 1768 1900 2389 2605 2628
Size: 331 1011 426 132 489 216 23
Fragments arranged by size:
1011 489 426 331 216 132 23
EcoO109I rG'GnC_Cy
Cuts at: 0 12 212 324 435 459 467 833 981
Size: 12 200 112 111 24 8 366 148
Cuts at: 981 1060 1061 1429 1685 1929 2063 2064 2092
Size: 79 1 368 256 244 134 1 28
Cuts at: 2092 2184 2411 2523 2628
Size: 92 227 112 105
Fragments arranged by size:
368 366 256 244 227 200 148 134
112 112 111 105 92 79 28 24
12 8 1 1
FspI TGC'GCA
Cuts at: 0 2087 2628
Size: 2087 541
HaeII r_GCGC'y
Cuts at: 0 1553 2628
Size: 1553 1075
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 513 545 1700 1732 2628
Size: 1 32 480 32 1155 32 896
Fragments arranged by size:
1155 896 480 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1776 2628
Size: 1776 852
HindIII A'AGCT_T
Cuts at: 0 1732 2628
Size: 1732 896
KasI G'GCGC_C
Cuts at: 0 1549 2628
Size: 1549 1079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 1343 2628
Size: 170 1173 1285
Fragments arranged by size:
1285 1173 170
MscI TGG'CCA
Cuts at: 0 750 1225 1273 2161 2191 2628
Size: 750 475 48 888 30 437
Fragments arranged by size:
888 750 475 437 48 30
MslI CAynn'nnrTG
Cuts at: 0 1766 2335 2628
Size: 1766 569 293
Fragments arranged by size:
1766 569 293
MspA1I CmG'CkG
Cuts at: 0 383 404 690 2040 2312 2589 2628
Size: 383 21 286 1350 272 277 39
Fragments arranged by size:
1350 383 286 277 272 39 21
NaeI GCC'GGC
Cuts at: 0 2485 2628
Size: 2485 143
NarI GG'CG_CC
Cuts at: 0 1550 2628
Size: 1550 1078
NcoI C'CATG_G
Cuts at: 0 473 929 2628
Size: 473 456 1699
Fragments arranged by size:
1699 473 456
NgoMIV G'CCGG_C
Cuts at: 0 2483 2628
Size: 2483 145
NspI r_CATG'y
Cuts at: 0 111 319 870 2125 2151 2628
Size: 111 208 551 1255 26 477
Fragments arranged by size:
1255 551 477 208 111 26
PasI CC'CwG_GG
Cuts at: 0 102 306 1396 1666 2068 2628
Size: 102 204 1090 270 402 560
Fragments arranged by size:
1090 560 402 270 204 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 155 935 2040 2312 2628
Size: 155 780 1105 272 316
Fragments arranged by size:
1105 780 316 272 155
PfoI T'CCnGG_A
Cuts at: 0 343 2471 2628
Size: 343 2128 157
Fragments arranged by size:
2128 343 157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1099 1131 2628
Size: 1099 32 1497
Fragments arranged by size:
1497 1099 32
PpuMI rG'GwC_Cy
Cuts at: 0 435 459 981 1429 1929 2184 2628
Size: 435 24 522 448 500 255 444
Fragments arranged by size:
522 500 448 444 435 255 24
PshAI GACnn'nnGTC
Cuts at: 0 573 2628
Size: 573 2055
PspOMI G'GGCC_C
Cuts at: 0 466 1060 1684 2063 2092 2411 2628
Size: 466 594 624 379 29 319 217
Fragments arranged by size:
624 594 466 379 319 217 29
PstI C_TGCA'G
Cuts at: 0 671 1040 1622 2628
Size: 671 369 582 1006
Fragments arranged by size:
1006 671 582 369
PvuII CAG'CTG
Cuts at: 0 690 2040 2589 2628
Size: 690 1350 549 39
Fragments arranged by size:
1350 690 549 39
SacI G_AGCT'C
Cuts at: 0 545 2628
Size: 545 2083
SalI G'TCGA_C
Cuts at: 0 1774 2628
Size: 1774 854
SanDI GG'GwC_CC
Cuts at: 0 981 2628
Size: 981 1647
SbfI CC_TGCA'GG
Cuts at: 0 1622 2628
Size: 1622 1006
ScaI AGT'ACT
Cuts at: 0 256 2628
Size: 256 2372
SexAI A'CCwGG_T
Cuts at: 0 284 2628
Size: 284 2344
SfcI C'TryA_G
Cuts at: 0 667 1036 1515 1618 2406 2628
Size: 667 369 479 103 788 222
Fragments arranged by size:
788 667 479 369 222 103
SfoI GGC'GCC
Cuts at: 0 1551 2628
Size: 1551 1077
SmaI CCC'GGG
Cuts at: 0 370 505 994 2628
Size: 370 135 489 1634
Fragments arranged by size:
1634 489 370 135
SmlI C'TyrA_G
Cuts at: 0 1784 1924 2628
Size: 1784 140 704
Fragments arranged by size:
1784 704 140
SphI G_CATG'C
Cuts at: 0 111 319 2151 2628
Size: 111 208 1832 477
Fragments arranged by size:
1832 477 208 111
SrfI GCCC'GGGC
Cuts at: 0 994 2628
Size: 994 1634
StyI C'CwwG_G
Cuts at: 0 242 462 473 929 963 1011 1226 2628
Size: 242 220 11 456 34 48 215 1402
Fragments arranged by size:
1402 456 242 220 215 48 34 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 319 2628
Size: 319 2309
TatI w'GTAC_w
Cuts at: 0 254 2175 2628
Size: 254 1921 453
Fragments arranged by size:
1921 453 254
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 641 673 772 804 2628
Size: 641 32 99 32 1824
Fragments arranged by size:
1824 641 99 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 514 2628
Size: 514 2114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 694 798 1151 2313 2628
Size: 694 104 353 1162 315
Fragments arranged by size:
1162 694 353 315 104
XmaI C'CCGG_G
Cuts at: 0 368 503 992 2628
Size: 368 135 489 1636
Fragments arranged by size:
1636 489 368 135
XmnI GAAnn'nnTTC
Cuts at: 0 1891 2628
Size: 1891 737
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI AvrII BaeI BanI BanII BbeI BbvCI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII HindIII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfoI SmaI SmlI SphI SrfI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI BamHI BbsI BcgI BciVI
BclI BfrBI BlpI BmtI BsaI BsaAI BsaBI BsiWI
BsmBI BspEI BsrBI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI EcoRI EcoRV FalI FseI FspAI
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PsiI PspXI PsrI
PvuI RsrII SacII SapI SfiI SgfI SgrAI SnaBI
SpeI SspI StuI SwaI XbaI XhoI ZraI