Restriction map for
pF1KB1891
(Linear) (Six-base) MAPSORT of: KIBB1891.seq Check: 2008 from: 1 to: 4389
KIBB1891.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AccI GT'mk_AC
Cuts at: 0 967 1280 2608 4310 4369 4389
Size: 967 313 1328 1702 59 20
Fragments arranged by size:
1702 1328 967 313 59 20
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1064 1940 2213 3491 4389
Size: 540 524 876 273 1278 898
Fragments arranged by size:
1278 898 876 540 524 273
AflIII A'CryG_T
Cuts at: 0 970 1654 1942 4389
Size: 970 684 288 2447
Fragments arranged by size:
2447 970 684 288
AleI CACnn'nnGTG
Cuts at: 0 3778 4389
Size: 3778 611
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 792 824 3096 3128 4389
Size: 792 32 2272 32 1261
Fragments arranged by size:
2272 1261 792 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1237 4389
Size: 1237 3152
ApoI r'AATT_y
Cuts at: 0 268 378 561 637 2238 3030 3159 3168
Size: 268 110 183 76 1601 792 129 9
Cuts at: 3168 3315 3501 4133 4272 4389
Size: 147 186 632 139 117
Fragments arranged by size:
1601 792 632 268 186 183 147 139
129 117 110 76 9
AvaI C'yCGr_G
Cuts at: 0 1483 4389
Size: 1483 2906
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3797 3830 4389
Size: 3797 33 559
Fragments arranged by size:
3797 559 33
BanII G_rGCy'C
Cuts at: 0 1177 1825 1888 4389
Size: 1177 648 63 2501
Fragments arranged by size:
2501 1177 648 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 177 2267 4120 4389
Size: 56 121 2090 1853 269
Fragments arranged by size:
2090 1853 269 121 56
BbvCI CC'TCA_GC
Cuts at: 0 3752 4389
Size: 3752 637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4091 4125 4389
Size: 4091 34 264
Fragments arranged by size:
4091 264 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 449 4389
Size: 449 3940
BfrBI ATG'CAT
Cuts at: 0 862 4389
Size: 862 3527
BglII A'GATC_T
Cuts at: 0 420 1440 2077 2371 2629 2677 2806 3415
Size: 420 1020 637 294 258 48 129 609
Cuts at: 3415 4389
Size: 974
Fragments arranged by size:
1020 974 637 609 420 294 258 129
48
Bme1580I G_kGCm'C
Cuts at: 0 3785 4389
Size: 3785 604
BmrI ACTGGGnnnn_n'
Cuts at: 0 2436 2477 4389
Size: 2436 41 1912
Fragments arranged by size:
2436 1912 41
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2600 3802 4389
Size: 2600 1202 587
Fragments arranged by size:
2600 1202 587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 4389
Size: 620 3769
Bpu10I CC'TnA_GC
Cuts at: 0 3752 4389
Size: 3752 637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 1421 2811 4389
Size: 860 561 1390 1578
Fragments arranged by size:
1578 1390 860 561
BsaI GGTCTCn'nnnn_
Cuts at: 0 369 2600 4389
Size: 369 2231 1789
Fragments arranged by size:
2231 1789 369
BsaAI yAC'GTr
Cuts at: 0 973 4389
Size: 973 3416
BsaBI GATnn'nnATC
Cuts at: 0 1416 2634 3426 3721 4389
Size: 1416 1218 792 295 668
Fragments arranged by size:
1416 1218 792 668 295
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1822 2778 3553 4389
Size: 1822 956 775 836
Fragments arranged by size:
1822 956 836 775
BseYI C'CCAG_C
Cuts at: 0 3440 4389
Size: 3440 949
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1324 4389
Size: 1324 3065
BsiHKAI G_wGCw'C
Cuts at: 0 1177 1268 1437 4389
Size: 1177 91 169 2952
Fragments arranged by size:
2952 1177 169 91
BsmI GAATG_Cn'
Cuts at: 0 3663 4389
Size: 3663 726
Bsp1286I G_dGCh'C
Cuts at: 0 1177 1268 1437 1825 1888 3785 4389
Size: 1177 91 169 388 63 1897 604
Fragments arranged by size:
1897 1177 604 388 169 91 63
BspHI T'CATG_A
Cuts at: 0 372 4389
Size: 372 4017
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 948 3783 4389
Size: 948 2835 606
Fragments arranged by size:
2835 948 606
BsrDI GCAATG_nn'
Cuts at: 0 950 1356 3872 4389
Size: 950 406 2516 517
Fragments arranged by size:
2516 950 517 406
BsrGI T'GTAC_A
Cuts at: 0 101 631 1624 3471 4389
Size: 101 530 993 1847 918
Fragments arranged by size:
1847 993 918 530 101
BssSI C'ACGA_G
Cuts at: 0 2933 4389
Size: 2933 1456
BstBI TT'CG_AA
Cuts at: 0 830 4389
Size: 830 3559
BstEII G'GTnAC_C
Cuts at: 0 2402 4389
Size: 2402 1987
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1588 4389
Size: 1588 2801
BstYI r'GATC_y
Cuts at: 0 420 1009 1411 1440 2077 2371 2629 2677
Size: 420 589 402 29 637 294 258 48
Cuts at: 2677 2806 3415 4389
Size: 129 609 974
Fragments arranged by size:
974 637 609 589 420 402 294 258
129 48 29
BstZ17I GTA'TAC
Cuts at: 0 2609 4389
Size: 2609 1780
BtgI C'CryG_G
Cuts at: 0 3210 3231 4389
Size: 3210 21 1158
Fragments arranged by size:
3210 1158 21
BtsI GCAGTG_nn'
Cuts at: 0 266 2181 4094 4389
Size: 266 1915 1913 295
Fragments arranged by size:
1915 1913 295 266
DraI TTT'AAA
Cuts at: 0 267 4389
Size: 267 4122
EaeI y'GGCC_r
Cuts at: 0 4166 4389
Size: 4166 223
EarI CTCTTCn'nnn_
Cuts at: 0 673 1083 1128 2454 2646 4389
Size: 673 410 45 1326 192 1743
Fragments arranged by size:
1743 1326 673 410 192 45
EcoICRI GAG'CTC
Cuts at: 0 1175 4389
Size: 1175 3214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 620 1064 1940 2213 3491 4389
Size: 540 80 444 876 273 1278 898
Fragments arranged by size:
1278 898 876 540 444 273 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3790 4389
Size: 3790 599
EcoO109I rG'GnC_Cy
Cuts at: 0 2723 2819 4389
Size: 2723 96 1570
Fragments arranged by size:
2723 1570 96
EcoRI G'AATT_C
Cuts at: 0 3315 4389
Size: 3315 1074
EcoRV GAT'ATC
Cuts at: 0 1586 4197 4389
Size: 1586 2611 192
Fragments arranged by size:
2611 1586 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2675 4389
Size: 2675 1714
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 531 563 1570 1602 1858 1890 2055 2087
Size: 531 32 1007 32 256 32 165 32
Cuts at: 2087 2869 2901 3532 3564 4389
Size: 782 32 631 32 825
Fragments arranged by size:
1007 825 782 631 531 256 165 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3867 4389
Size: 3867 522
MfeI C'AATT_G
Cuts at: 0 1897 3063 3898 4389
Size: 1897 1166 835 491
Fragments arranged by size:
1897 1166 835 491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 2902 3750 3988 4389
Size: 835 2067 848 238 401
Fragments arranged by size:
2067 848 835 401 238
MscI TGG'CCA
Cuts at: 0 4168 4389
Size: 4168 221
MslI CAynn'nnrTG
Cuts at: 0 2126 3014 3050 3778 4389
Size: 2126 888 36 728 611
Fragments arranged by size:
2126 888 728 611 36
MspA1I CmG'CkG
Cuts at: 0 315 1084 3440 4389
Size: 315 769 2356 949
Fragments arranged by size:
2356 949 769 315
NsiI A_TGCA'T
Cuts at: 0 864 4389
Size: 864 3525
NspI r_CATG'y
Cuts at: 0 1658 1912 1946 4389
Size: 1658 254 34 2443
Fragments arranged by size:
2443 1658 254 34
PciI A'CATG_T
Cuts at: 0 1654 1942 4389
Size: 1654 288 2447
Fragments arranged by size:
2447 1654 288
PflMI CCAn_nnn'nTGG
Cuts at: 0 2792 3520 4009 4389
Size: 2792 728 489 380
Fragments arranged by size:
2792 728 489 380
PpuMI rG'GwC_Cy
Cuts at: 0 2723 2819 4389
Size: 2723 96 1570
Fragments arranged by size:
2723 1570 96
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1613 1645 3900 3932 4389
Size: 1613 32 2255 32 457
Fragments arranged by size:
2255 1613 457 32 32
PvuII CAG'CTG
Cuts at: 0 315 1084 3440 4389
Size: 315 769 2356 949
Fragments arranged by size:
2356 949 769 315
SacI G_AGCT'C
Cuts at: 0 1177 4389
Size: 1177 3212
SfcI C'TryA_G
Cuts at: 0 618 1178 2081 4389
Size: 618 560 903 2308
Fragments arranged by size:
2308 903 618 560
SmlI C'TyrA_G
Cuts at: 0 875 1436 1483 2826 4389
Size: 875 561 47 1343 1563
Fragments arranged by size:
1563 1343 875 561 47
SspI AAT'ATT
Cuts at: 0 1542 1977 4324 4389
Size: 1542 435 2347 65
Fragments arranged by size:
2347 1542 435 65
StyI C'CwwG_G
Cuts at: 0 4202 4389
Size: 4202 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1022 2080 2421 2582 3688 4389
Size: 1022 1058 341 161 1106 701
Fragments arranged by size:
1106 1058 1022 701 341 161
TatI w'GTAC_w
Cuts at: 0 101 541 631 1297 1624 3471 3943 4389
Size: 101 440 90 666 327 1847 472 446
Fragments arranged by size:
1847 666 472 446 440 327 101 90
XbaI T'CTAG_A
Cuts at: 0 2374 2662 3012 4389
Size: 2374 288 350 1377
Fragments arranged by size:
2374 1377 350 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2396 3521 3592 4389
Size: 2396 1125 71 797
Fragments arranged by size:
2396 1125 797 71
XhoI C'TCGA_G
Cuts at: 0 1483 4389
Size: 1483 2906
XmnI GAAnn'nnTTC
Cuts at: 0 770 1541 2111 3504 3682 4389
Size: 770 771 570 1393 178 707
Fragments arranged by size:
1393 771 770 707 570 178
Enzymes that do cut:
AccI AcuI AflIII AleI AloI AlwNI ApoI AvaI
BaeI BanII BbsI BbvCI BcgI BciVI BfrBI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII MfeI
MmeI MscI MslI MspA1I NsiI NspI PciI PflMI
PpuMI PsrI PvuII SacI SfcI SmlI SspI StyI
TaqII TatI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BamHI
BanI BbeI BclI BglI BlpI BmgBI BmtI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BstAPI Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EciI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TstI Tth111I XmaI ZraI