Restriction map for
pF1KB1630
(Linear) (Six-base) MAPSORT of: KIBB1630.seq Check: 7768 from: 1 to: 3156
KIBB1630.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 715 1270 3156
Size: 715 555 1886
Fragments arranged by size:
1886 715 555
Acc65I G'GTAC_C
Cuts at: 0 753 2397 3156
Size: 753 1644 759
Fragments arranged by size:
1644 759 753
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1497 1744 2864 3156
Size: 1003 494 247 1120 292
Fragments arranged by size:
1120 1003 494 292 247
AfeI AGC'GCT
Cuts at: 0 150 3156
Size: 150 3006
AflII C'TTAA_G
Cuts at: 0 2965 3156
Size: 2965 191
AflIII A'CryG_T
Cuts at: 0 1675 1977 3156
Size: 1675 302 1179
Fragments arranged by size:
1675 1179 302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 149 3156
Size: 149 3007
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 2308 2340 3156
Size: 391 32 1885 32 816
Fragments arranged by size:
1885 816 391 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1402 1923 3156
Size: 1402 521 1233
Fragments arranged by size:
1402 1233 521
ApoI r'AATT_y
Cuts at: 0 739 895 976 2363 2729 3156
Size: 739 156 81 1387 366 427
Fragments arranged by size:
1387 739 427 366 156 81
AseI AT'TA_AT
Cuts at: 0 1282 3156
Size: 1282 1874
AvaI C'yCGr_G
Cuts at: 0 37 3156
Size: 37 3119
AvrII C'CTAG_G
Cuts at: 0 624 3156
Size: 624 2532
BanI G'GyrC_C
Cuts at: 0 368 753 2397 3156
Size: 368 385 1644 759
Fragments arranged by size:
1644 759 385 368
BanII G_rGCy'C
Cuts at: 0 2681 2990 3156
Size: 2681 309 166
Fragments arranged by size:
2681 309 166
BbeI G_GCGC'C
Cuts at: 0 372 3156
Size: 372 2784
BbsI GAAGACnn'nnnn_
Cuts at: 0 340 512 2056 2156 3156
Size: 340 172 1544 100 1000
Fragments arranged by size:
1544 1000 340 172 100
BbvCI CC'TCA_GC
Cuts at: 0 1246 3156
Size: 1246 1910
BciVI GTATCCnnnnn_n'
Cuts at: 0 613 2109 3156
Size: 613 1496 1047
Fragments arranged by size:
1496 1047 613
BfrBI ATG'CAT
Cuts at: 0 1428 1630 2236 3156
Size: 1428 202 606 920
Fragments arranged by size:
1428 920 606 202
BglI GCCn_nnn'nGGC
Cuts at: 0 113 3156
Size: 113 3043
BglII A'GATC_T
Cuts at: 0 766 1755 3156
Size: 766 989 1401
Fragments arranged by size:
1401 989 766
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1229 3156
Size: 1229 1927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2612 2665 2974 3156
Size: 2612 53 309 182
Fragments arranged by size:
2612 309 182 53
Bpu10I CC'TnA_GC
Cuts at: 0 1246 2411 3156
Size: 1246 1165 745
Fragments arranged by size:
1246 1165 745
BsaBI GATnn'nnATC
Cuts at: 0 1427 1742 1919 3156
Size: 1427 315 177 1237
Fragments arranged by size:
1427 1237 315 177
BsaHI Gr'CG_yC
Cuts at: 0 116 164 369 3156
Size: 116 48 205 2787
Fragments arranged by size:
2787 205 116 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 460 2276 2930 3009 3156
Size: 460 1816 654 79 147
Fragments arranged by size:
1816 654 460 147 79
BsiEI CG_ry'CG
Cuts at: 0 14 71 3156
Size: 14 57 3085
Fragments arranged by size:
3085 57 14
BsiHKAI G_wGCw'C
Cuts at: 0 1779 2681 2990 3156
Size: 1779 902 309 166
Fragments arranged by size:
1779 902 309 166
BsmI GAATG_Cn'
Cuts at: 0 2176 3156
Size: 2176 980
BsmBI CGTCTCn'nnnn_
Cuts at: 0 809 3156
Size: 809 2347
Bsp1286I G_dGCh'C
Cuts at: 0 1779 2681 2990 3156
Size: 1779 902 309 166
Fragments arranged by size:
1779 902 309 166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 330 1571 3156
Size: 330 1241 1585
Fragments arranged by size:
1585 1241 330
BsrDI GCAATG_nn'
Cuts at: 0 2987 3156
Size: 2987 169
BssHII G'CGCG_C
Cuts at: 0 44 3156
Size: 44 3112
BssSI C'ACGA_G
Cuts at: 0 1499 3156
Size: 1499 1657
BstBI TT'CG_AA
Cuts at: 0 2141 3156
Size: 2141 1015
BstYI r'GATC_y
Cuts at: 0 228 766 1755 3156
Size: 228 538 989 1401
Fragments arranged by size:
1401 989 538 228
BstZ17I GTA'TAC
Cuts at: 0 1271 3156
Size: 1271 1885
BtgI C'CryG_G
Cuts at: 0 8 3156
Size: 8 3148
ClaI AT'CG_AT
Cuts at: 0 2021 3156
Size: 2021 1135
DraIII CAC_nnn'GTG
Cuts at: 0 2186 3156
Size: 2186 970
EaeI y'GGCC_r
Cuts at: 0 11 143 3156
Size: 11 132 3013
Fragments arranged by size:
3013 132 11
EagI C'GGCC_G
Cuts at: 0 11 3156
Size: 11 3145
EarI CTCTTCn'nnn_
Cuts at: 0 449 641 656 1022 2258 2480 3156
Size: 449 192 15 366 1236 222 676
Fragments arranged by size:
1236 676 449 366 222 192 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 11 3156
Size: 11 3145
EcoICRI GAG'CTC
Cuts at: 0 2679 2988 3156
Size: 2679 309 168
Fragments arranged by size:
2679 309 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1497 1744 2612 2665 2864 2974 3156
Size: 1003 494 247 868 53 199 110 182
Fragments arranged by size:
1003 868 494 247 199 182 110 53
EcoRI G'AATT_C
Cuts at: 0 739 976 2729 3156
Size: 739 237 1753 427
Fragments arranged by size:
1753 739 427 237
EcoRV GAT'ATC
Cuts at: 0 2019 3156
Size: 2019 1137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 417 821 1774 1924 2467 2524 3156
Size: 417 404 953 150 543 57 632
Fragments arranged by size:
953 632 543 417 404 150 57
HaeII r_GCGC'y
Cuts at: 0 152 372 3156
Size: 152 220 2784
Fragments arranged by size:
2784 220 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 499 531 563 1027 1059 1493
Size: 178 32 289 32 32 464 32 434
Cuts at: 1493 1525 2107 2139 2688 2720 3156
Size: 32 582 32 549 32 436
Fragments arranged by size:
582 549 464 436 434 289 178 32
32 32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2506 3156
Size: 2506 650
KasI G'GCGC_C
Cuts at: 0 368 3156
Size: 368 2788
KpnI G_GTAC'C
Cuts at: 0 757 2401 3156
Size: 757 1644 755
Fragments arranged by size:
1644 757 755
MfeI C'AATT_G
Cuts at: 0 2529 3156
Size: 2529 627
MluI A'CGCG_T
Cuts at: 0 1675 3156
Size: 1675 1481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1565 3156
Size: 364 1201 1591
Fragments arranged by size:
1591 1201 364
MslI CAynn'nnrTG
Cuts at: 0 1348 3156
Size: 1348 1808
MspA1I CmG'CkG
Cuts at: 0 10 77 2653 2802 3156
Size: 10 67 2576 149 354
Fragments arranged by size:
2576 354 149 67 10
NarI GG'CG_CC
Cuts at: 0 369 3156
Size: 369 2787
NdeI CA'TA_TG
Cuts at: 0 695 1154 2814 3156
Size: 695 459 1660 342
Fragments arranged by size:
1660 695 459 342
NsiI A_TGCA'T
Cuts at: 0 1430 1632 2238 3156
Size: 1430 202 606 918
Fragments arranged by size:
1430 918 606 202
NspI r_CATG'y
Cuts at: 0 1560 1981 3156
Size: 1560 421 1175
Fragments arranged by size:
1560 1175 421
PciI A'CATG_T
Cuts at: 0 1977 3156
Size: 1977 1179
PfoI T'CCnGG_A
Cuts at: 0 2681 3156
Size: 2681 475
PsiI TTA'TAA
Cuts at: 0 1822 2135 3156
Size: 1822 313 1021
Fragments arranged by size:
1822 1021 313
PstI C_TGCA'G
Cuts at: 0 1582 2058 3156
Size: 1582 476 1098
Fragments arranged by size:
1582 1098 476
PvuI CG_AT'CG
Cuts at: 0 71 3156
Size: 71 3085
PvuII CAG'CTG
Cuts at: 0 2653 2802 3156
Size: 2653 149 354
Fragments arranged by size:
2653 354 149
SacI G_AGCT'C
Cuts at: 0 2681 2990 3156
Size: 2681 309 166
Fragments arranged by size:
2681 309 166
SacII CC_GC'GG
Cuts at: 0 11 3156
Size: 11 3145
SapI GCTCTTCn'nnn_
Cuts at: 0 449 2258 3156
Size: 449 1809 898
Fragments arranged by size:
1809 898 449
ScaI AGT'ACT
Cuts at: 0 1505 1576 3156
Size: 1505 71 1580
Fragments arranged by size:
1580 1505 71
SexAI A'CCwGG_T
Cuts at: 0 1401 3156
Size: 1401 1755
SfcI C'TryA_G
Cuts at: 0 1578 1835 2054 2154 3156
Size: 1578 257 219 100 1002
Fragments arranged by size:
1578 1002 257 219 100
SfoI GGC'GCC
Cuts at: 0 370 3156
Size: 370 2786
SmlI C'TyrA_G
Cuts at: 0 2965 3156
Size: 2965 191
SpeI A'CTAG_T
Cuts at: 0 1137 3156
Size: 1137 2019
SspI AAT'ATT
Cuts at: 0 182 2647 3156
Size: 182 2465 509
Fragments arranged by size:
2465 509 182
StyI C'CwwG_G
Cuts at: 0 624 1122 2279 3156
Size: 624 498 1157 877
Fragments arranged by size:
1157 877 624 498
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1787 2292 3156
Size: 1787 505 864
Fragments arranged by size:
1787 864 505
TatI w'GTAC_w
Cuts at: 0 1503 1574 3156
Size: 1503 71 1582
Fragments arranged by size:
1582 1503 71
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2979 3156
Size: 2979 177
XmnI GAAnn'nnTTC
Cuts at: 0 1949 2252 3156
Size: 1949 303 904
Fragments arranged by size:
1949 904 303
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AlfI AloI
AlwNI ApoI AseI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BciVI BfrBI BglI BglII BplI BpmI
Bpu10I BsaBI BsaHI BseRI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BssHII BssSI BstBI BstYI BstZ17I
BtgI ClaI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI EcoRV FalI HaeII Hin4I HindIII KasI
KpnI MfeI MluI MmeI MslI MspA1I NarI NdeI
NsiI NspI PciI PfoI PsiI PstI PvuI PvuII
SacI SacII SapI ScaI SexAI SfcI SfoI SmlI
SpeI SspI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AgeI AhdI AleI ApaI ApaLI
AscI AsiSI BaeI BamHI BcgI BclI BlpI Bme1580I
BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaWI BsaXI
BseYI BsgI BsiWI BspEI BspHI BsrBI BsrFI BsrGI
BstAPI BstEII BstXI Bsu36I BtgZI BtsI CspCI DraI
DrdI EcoNI EcoO109I FseI FspI FspAI HincII HpaI
MscI NaeI NcoI NgoMIV NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI RsrII SalI SanDI SbfI SfiI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI