Restriction map for
pF1KB1569
(Linear) (Six-base) MAPSORT of: KIBB1569.seq Check: 4133 from: 1 to: 2949
KIBB1569.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 902 1876 2949
Size: 902 974 1073
Fragments arranged by size:
1073 974 902
AccI GT'mk_AC
Cuts at: 0 590 819 2949
Size: 590 229 2130
Fragments arranged by size:
2130 590 229
Acc65I G'GTAC_C
Cuts at: 0 645 2949
Size: 645 2304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 2683 2949
Size: 505 2178 266
Fragments arranged by size:
2178 505 266
AhdI GACnn_n'nnGTC
Cuts at: 0 660 2949
Size: 660 2289
AleI CACnn'nnGTG
Cuts at: 0 150 1177 2800 2949
Size: 150 1027 1623 149
Fragments arranged by size:
1623 1027 150 149
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 283 315 2949
Size: 283 32 2634
Fragments arranged by size:
2634 283 32
AlwNI CAG_nnn'CTG
Cuts at: 0 666 1543 2329 2949
Size: 666 877 786 620
Fragments arranged by size:
877 786 666 620
ApoI r'AATT_y
Cuts at: 0 400 1891 2949
Size: 400 1491 1058
Fragments arranged by size:
1491 1058 400
BanI G'GyrC_C
Cuts at: 0 645 2949
Size: 645 2304
BanII G_rGCy'C
Cuts at: 0 296 1258 1533 2949
Size: 296 962 275 1416
Fragments arranged by size:
1416 962 296 275
BbsI GAAGACnn'nnnn_
Cuts at: 0 1814 2949
Size: 1814 1135
BclI T'GATC_A
Cuts at: 0 387 2258 2949
Size: 387 1871 691
Fragments arranged by size:
1871 691 387
BlpI GC'TnA_GC
Cuts at: 0 1252 2949
Size: 1252 1697
Bme1580I G_kGCm'C
Cuts at: 0 607 871 2949
Size: 607 264 2078
Fragments arranged by size:
2078 607 264
BmgBI CAC'GTC
Cuts at: 0 2405 2949
Size: 2405 544
BmrI ACTGGGnnnn_n'
Cuts at: 0 264 654 2142 2484 2932 2949
Size: 264 390 1488 342 448 17
Fragments arranged by size:
1488 448 390 342 264 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1272 2949
Size: 1272 1677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1053 1329 2332 2949
Size: 1053 276 1003 617
Fragments arranged by size:
1053 1003 617 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1480 2949
Size: 280 1200 1469
Fragments arranged by size:
1469 1200 280
BsaI GGTCTCn'nnnn_
Cuts at: 0 1006 1780 2228 2460 2949
Size: 1006 774 448 232 489
Fragments arranged by size:
1006 774 489 448 232
BsaAI yAC'GTr
Cuts at: 0 1515 2134 2949
Size: 1515 619 815
Fragments arranged by size:
1515 815 619
BsaBI GATnn'nnATC
Cuts at: 0 386 2359 2949
Size: 386 1973 590
Fragments arranged by size:
1973 590 386
BsaWI w'CCGG_w
Cuts at: 0 2645 2949
Size: 2645 304
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 283 313 957 987 1582 1612 1686 1716
Size: 283 30 644 30 595 30 74 30
Cuts at: 1716 2437 2467 2949
Size: 721 30 482
Fragments arranged by size:
721 644 595 482 283 74 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 1654 2004 2360 2949
Size: 13 1641 350 356 589
Fragments arranged by size:
1641 589 356 350 13
BseYI C'CCAG_C
Cuts at: 0 123 2547 2949
Size: 123 2424 402
Fragments arranged by size:
2424 402 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1926 2699 2949
Size: 1926 773 250
Fragments arranged by size:
1926 773 250
BsiHKAI G_wGCw'C
Cuts at: 0 296 1258 2949
Size: 296 962 1691
Fragments arranged by size:
1691 962 296
BsmI GAATG_Cn'
Cuts at: 0 323 2949
Size: 323 2626
Bsp1286I G_dGCh'C
Cuts at: 0 296 607 871 1258 1533 2949
Size: 296 311 264 387 275 1416
Fragments arranged by size:
1416 387 311 296 275 264
BspEI T'CCGG_A
Cuts at: 0 2645 2949
Size: 2645 304
BspHI T'CATG_A
Cuts at: 0 1628 1827 2949
Size: 1628 199 1122
Fragments arranged by size:
1628 1122 199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 902 1876 2949
Size: 902 974 1073
Fragments arranged by size:
1073 974 902
BsrDI GCAATG_nn'
Cuts at: 0 219 434 783 1758 2724 2949
Size: 219 215 349 975 966 225
Fragments arranged by size:
975 966 349 225 219 215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1845 2366 2949
Size: 1845 521 583
Fragments arranged by size:
1845 583 521
BstYI r'GATC_y
Cuts at: 0 131 465 711 2354 2949
Size: 131 334 246 1643 595
Fragments arranged by size:
1643 595 334 246 131
BstZ17I GTA'TAC
Cuts at: 0 591 2949
Size: 591 2358
BtgI C'CryG_G
Cuts at: 0 650 956 1175 2537 2870 2949
Size: 650 306 219 1362 333 79
Fragments arranged by size:
1362 650 333 306 219 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2429 2949
Size: 2429 520
BtsI GCAGTG_nn'
Cuts at: 0 1914 2778 2949
Size: 1914 864 171
Fragments arranged by size:
1914 864 171
DraI TTT'AAA
Cuts at: 0 1763 2949
Size: 1763 1186
DrdI GACnn_nn'nnGTC
Cuts at: 0 1411 2949
Size: 1411 1538
EaeI y'GGCC_r
Cuts at: 0 1949 2949
Size: 1949 1000
EarI CTCTTCn'nnn_
Cuts at: 0 152 2949
Size: 152 2797
EcoICRI GAG'CTC
Cuts at: 0 294 1256 2949
Size: 294 962 1693
Fragments arranged by size:
1693 962 294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1053 1329 2332 2683 2949
Size: 505 548 276 1003 351 266
Fragments arranged by size:
1003 548 505 351 276 266
EcoNI CCTnn'n_nnAGG
Cuts at: 0 670 2949
Size: 670 2279
EcoO109I rG'GnC_Cy
Cuts at: 0 258 514 2949
Size: 258 256 2435
Fragments arranged by size:
2435 258 256
EcoRV GAT'ATC
Cuts at: 0 2727 2949
Size: 2727 222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2808 2949
Size: 2808 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 646 678 937 969 1327 1359
Size: 123 32 491 32 259 32 358 32
Cuts at: 1359 1582 1614 2339 2371 2437 2469 2472 2504
Size: 223 32 725 32 66 32 3 32
Cuts at: 2504 2949
Size: 445
Fragments arranged by size:
725 491 445 358 259 223 123 66
32 32 32 32 32 32 32 32
3
HincII GTy'rAC
Cuts at: 0 522 693 820 1791 2949
Size: 522 171 127 971 1158
Fragments arranged by size:
1158 971 522 171 127
HindIII A'AGCT_T
Cuts at: 0 812 2638 2949
Size: 812 1826 311
Fragments arranged by size:
1826 812 311
KpnI G_GTAC'C
Cuts at: 0 649 2949
Size: 649 2300
MfeI C'AATT_G
Cuts at: 0 237 2949
Size: 237 2712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1860 2949
Size: 1860 1089
MscI TGG'CCA
Cuts at: 0 1951 2949
Size: 1951 998
MslI CAynn'nnrTG
Cuts at: 0 150 460 1177 1388 1802 2800 2949
Size: 150 310 717 211 414 998 149
Fragments arranged by size:
998 717 414 310 211 150 149
MspA1I CmG'CkG
Cuts at: 0 35 442 1543 1645 2551 2565 2949
Size: 35 407 1101 102 906 14 384
Fragments arranged by size:
1101 906 407 384 102 35 14
NcoI C'CATG_G
Cuts at: 0 650 956 2537 2949
Size: 650 306 1581 412
Fragments arranged by size:
1581 650 412 306
NdeI CA'TA_TG
Cuts at: 0 1084 1803 2949
Size: 1084 719 1146
Fragments arranged by size:
1146 1084 719
PflMI CCAn_nnn'nTGG
Cuts at: 0 148 666 2949
Size: 148 518 2283
Fragments arranged by size:
2283 518 148
PfoI T'CCnGG_A
Cuts at: 0 1025 2309 2949
Size: 1025 1284 640
Fragments arranged by size:
1284 1025 640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 2949
Size: 283 32 2634
Fragments arranged by size:
2634 283 32
PpuMI rG'GwC_Cy
Cuts at: 0 258 514 2949
Size: 258 256 2435
Fragments arranged by size:
2435 258 256
PshAI GACnn'nnGTC
Cuts at: 0 227 2949
Size: 227 2722
PstI C_TGCA'G
Cuts at: 0 733 913 2949
Size: 733 180 2036
Fragments arranged by size:
2036 733 180
PvuII CAG'CTG
Cuts at: 0 35 442 2551 2565 2949
Size: 35 407 2109 14 384
Fragments arranged by size:
2109 407 384 35 14
SacI G_AGCT'C
Cuts at: 0 296 1258 2949
Size: 296 962 1691
Fragments arranged by size:
1691 962 296
SalI G'TCGA_C
Cuts at: 0 818 2949
Size: 818 2131
SanDI GG'GwC_CC
Cuts at: 0 258 2949
Size: 258 2691
SexAI A'CCwGG_T
Cuts at: 0 203 822 2949
Size: 203 619 2127
Fragments arranged by size:
2127 619 203
SfcI C'TryA_G
Cuts at: 0 729 909 2384 2507 2949
Size: 729 180 1475 123 442
Fragments arranged by size:
1475 729 442 180 123
SmlI C'TyrA_G
Cuts at: 0 295 1495 2949
Size: 295 1200 1454
Fragments arranged by size:
1454 1200 295
SnaBI TAC'GTA
Cuts at: 0 1515 2134 2949
Size: 1515 619 815
Fragments arranged by size:
1515 815 619
SpeI A'CTAG_T
Cuts at: 0 1582 2949
Size: 1582 1367
StyI C'CwwG_G
Cuts at: 0 572 650 718 956 1838 2117 2537 2949
Size: 572 78 68 238 882 279 420 412
Fragments arranged by size:
882 572 420 412 279 238 78 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 140 204 1088 2949
Size: 140 64 884 1861
Fragments arranged by size:
1861 884 140 64
TatI w'GTAC_w
Cuts at: 0 365 725 733 2807 2949
Size: 365 360 8 2074 142
Fragments arranged by size:
2074 365 360 142 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1686 1718 2949
Size: 1686 32 1231
Fragments arranged by size:
1686 1231 32
XbaI T'CTAG_A
Cuts at: 0 2913 2949
Size: 2913 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 149 2949
Size: 149 2800
XmnI GAAnn'nnTTC
Cuts at: 0 65 2949
Size: 65 2884
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AleI AloI AlwNI
ApoI BanI BanII BbsI BclI BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NcoI NdeI PflMI PfoI
PpiI PpuMI PshAI PstI PvuII SacI SalI SanDI
SexAI SfcI SmlI SnaBI SpeI StyI TaqII TatI
TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AlfI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BmtI
Bpu10I BsaHI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII Bsu36I ClaI CspCI
DraIII EagI EciI EcoRI FseI FspI FspAI HaeII
HpaI KasI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SphI
SrfI SspI StuI SwaI Tth111I XhoI XmaI ZraI