Restriction map for
pF1KB1529
(Linear) (Six-base) MAPSORT of: KIBB1529.seq Check: 5421 from: 1 to: 3906
KIBB1529.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 85 3906
Size: 85 3821
AccI GT'mk_AC
Cuts at: 0 2224 3906
Size: 2224 1682
Acc65I G'GTAC_C
Cuts at: 0 763 3906
Size: 763 3143
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 776 1202 2226 2288 2504 3625 3906
Size: 689 87 426 1024 62 216 1121 281
Fragments arranged by size:
1121 1024 689 426 281 216 87 62
AflII C'TTAA_G
Cuts at: 0 2539 3906
Size: 2539 1367
AflIII A'CryG_T
Cuts at: 0 3007 3906
Size: 3007 899
AgeI A'CCGG_T
Cuts at: 0 1191 3906
Size: 1191 2715
AhdI GACnn_n'nnGTC
Cuts at: 0 1258 3906
Size: 1258 2648
AleI CACnn'nnGTG
Cuts at: 0 3574 3906
Size: 3574 332
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2615 3906
Size: 2615 1291
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 592 624 3906
Size: 592 32 3282
Fragments arranged by size:
3282 592 32
AlwNI CAG_nnn'CTG
Cuts at: 0 19 1225 1696 1951 2976 3603 3906
Size: 19 1206 471 255 1025 627 303
Fragments arranged by size:
1206 1025 627 471 303 255 19
ApoI r'AATT_y
Cuts at: 0 522 1678 2014 2347 3106 3906
Size: 522 1156 336 333 759 800
Fragments arranged by size:
1156 800 759 522 336 333
AvaI C'yCGr_G
Cuts at: 0 3765 3906
Size: 3765 141
AvrII C'CTAG_G
Cuts at: 0 285 687 3906
Size: 285 402 3219
Fragments arranged by size:
3219 402 285
BamHI G'GATC_C
Cuts at: 0 846 1402 2752 3906
Size: 846 556 1350 1154
Fragments arranged by size:
1350 1154 846 556
BanI G'GyrC_C
Cuts at: 0 763 3031 3821 3906
Size: 763 2268 790 85
Fragments arranged by size:
2268 790 763 85
BanII G_rGCy'C
Cuts at: 0 1234 3906
Size: 1234 2672
BbsI GAAGACnn'nnnn_
Cuts at: 0 2232 2288 2420 3906
Size: 2232 56 132 1486
Fragments arranged by size:
2232 1486 132 56
BbvCI CC'TCA_GC
Cuts at: 0 1243 2760 3906
Size: 1243 1517 1146
Fragments arranged by size:
1517 1243 1146
BciVI GTATCCnnnnn_n'
Cuts at: 0 722 974 3906
Size: 722 252 2932
Fragments arranged by size:
2932 722 252
BclI T'GATC_A
Cuts at: 0 3161 3906
Size: 3161 745
BfrBI ATG'CAT
Cuts at: 0 3382 3906
Size: 3382 524
BglII A'GATC_T
Cuts at: 0 1684 2439 2535 3906
Size: 1684 755 96 1371
Fragments arranged by size:
1684 1371 755 96
BlpI GC'TnA_GC
Cuts at: 0 854 3906
Size: 854 3052
Bme1580I G_kGCm'C
Cuts at: 0 1485 3414 3824 3906
Size: 1485 1929 410 82
Fragments arranged by size:
1929 1485 410 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 710 3906
Size: 710 3196
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3766 3906
Size: 3766 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 320 548 1616 2325 3245 3577 3906
Size: 302 18 228 1068 709 920 332 329
Fragments arranged by size:
1068 920 709 332 329 302 228 18
Bpu10I CC'TnA_GC
Cuts at: 0 1020 1243 2760 3725 3906
Size: 1020 223 1517 965 181
Fragments arranged by size:
1517 1020 965 223 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 3023 3479 3906
Size: 98 2925 456 427
Fragments arranged by size:
2925 456 427 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 162 2866 3278 3906
Size: 162 2704 412 628
Fragments arranged by size:
2704 628 412 162
BsaAI yAC'GTr
Cuts at: 0 3008 3906
Size: 3008 898
BsaHI Gr'CG_yC
Cuts at: 0 420 3906
Size: 420 3486
BsaWI w'CCGG_w
Cuts at: 0 1191 3906
Size: 1191 2715
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 592 622 918 948 2323 2353 2992 3022
Size: 592 30 296 30 1375 30 639 30
Cuts at: 3022 3906
Size: 884
Fragments arranged by size:
1375 884 639 592 296 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 616 2546 3228 3783 3906
Size: 616 1930 682 555 123
Fragments arranged by size:
1930 682 616 555 123
BseYI C'CCAG_C
Cuts at: 0 1140 1466 2883 3013 3018 3555 3906
Size: 1140 326 1417 130 5 537 351
Fragments arranged by size:
1417 1140 537 351 326 130 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3600 3906
Size: 3600 306
BsiHKAI G_wGCw'C
Cuts at: 0 2668 3906
Size: 2668 1238
BsmI GAATG_Cn'
Cuts at: 0 281 869 3778 3906
Size: 281 588 2909 128
Fragments arranged by size:
2909 588 281 128
Bsp1286I G_dGCh'C
Cuts at: 0 1234 1485 2668 3414 3824 3906
Size: 1234 251 1183 746 410 82
Fragments arranged by size:
1234 1183 746 410 251 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 85 2984 3906
Size: 85 2899 922
Fragments arranged by size:
2899 922 85
BsrDI GCAATG_nn'
Cuts at: 0 1118 1519 3906
Size: 1118 401 2387
Fragments arranged by size:
2387 1118 401
BsrFI r'CCGG_y
Cuts at: 0 1191 3906
Size: 1191 2715
BsrGI T'GTAC_A
Cuts at: 0 3530 3906
Size: 3530 376
BstEII G'GTnAC_C
Cuts at: 0 3537 3906
Size: 3537 369
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 2181 3549 3906
Size: 723 1458 1368 357
Fragments arranged by size:
1458 1368 723 357
BstYI r'GATC_y
Cuts at: 0 846 1402 1599 1684 2439 2535 2752 3906
Size: 846 556 197 85 755 96 217 1154
Fragments arranged by size:
1154 846 755 556 217 197 96 85
Bsu36I CC'TnA_GG
Cuts at: 0 1788 3906
Size: 1788 2118
BtgI C'CryG_G
Cuts at: 0 41 3906
Size: 41 3865
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 554 3906
Size: 554 3352
BtsI GCAGTG_nn'
Cuts at: 0 468 770 2396 2469 2666 3569 3581 3588
Size: 468 302 1626 73 197 903 12 7
Cuts at: 3588 3906
Size: 318
Fragments arranged by size:
1626 903 468 318 302 197 73 12
7
ClaI AT'CG_AT
Cuts at: 0 3614 3906
Size: 3614 292
DraI TTT'AAA
Cuts at: 0 1130 1551 1719 2901 3906
Size: 1130 421 168 1182 1005
Fragments arranged by size:
1182 1130 1005 421 168
DrdI GACnn_nn'nnGTC
Cuts at: 0 2220 3906
Size: 2220 1686
EaeI y'GGCC_r
Cuts at: 0 10 675 725 2586 3906
Size: 10 665 50 1861 1320
Fragments arranged by size:
1861 1320 665 50 10
EarI CTCTTCn'nnn_
Cuts at: 0 356 425 481 809 2870 3906
Size: 356 69 56 328 2061 1036
Fragments arranged by size:
2061 1036 356 328 69 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 51 3906
Size: 51 3855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 320 548 689 776 1202 1616 2226
Size: 302 18 228 141 87 426 414 610
Cuts at: 2226 2288 2325 2504 3245 3577 3625 3906
Size: 62 37 179 741 332 48 281
Fragments arranged by size:
741 610 426 414 332 302 281 228
179 141 87 62 48 37 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 92 116 284 2146 3906
Size: 92 24 168 1862 1760
Fragments arranged by size:
1862 1760 168 92 24
EcoO109I rG'GnC_Cy
Cuts at: 0 223 589 1222 2973 3906
Size: 223 366 633 1751 933
Fragments arranged by size:
1751 933 633 366 223
EcoRI G'AATT_C
Cuts at: 0 1678 2014 3906
Size: 1678 336 1892
Fragments arranged by size:
1892 1678 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 933 1078 2881 3266 3906
Size: 933 145 1803 385 640
Fragments arranged by size:
1803 933 640 385 145
HaeII r_GCGC'y
Cuts at: 0 7 3906
Size: 7 3899
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1244 1276 1558 1590 1720 1752 2323 2355
Size: 1244 32 282 32 130 32 571 32
Cuts at: 2355 2407 2439 2479 2511 3661 3676 3693 3708
Size: 52 32 40 32 1150 15 17 15
Cuts at: 3708 3906
Size: 198
Fragments arranged by size:
1244 1150 571 282 198 130 52 40
32 32 32 32 32 32 17 15
15
HincII GTy'rAC
Cuts at: 0 1943 3906
Size: 1943 1963
HindIII A'AGCT_T
Cuts at: 0 1668 3906
Size: 1668 2238
KpnI G_GTAC'C
Cuts at: 0 767 3906
Size: 767 3139
MfeI C'AATT_G
Cuts at: 0 809 3906
Size: 809 3097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1180 1260 3906
Size: 961 219 80 2646
Fragments arranged by size:
2646 961 219 80
MscI TGG'CCA
Cuts at: 0 677 727 2588 3906
Size: 677 50 1861 1318
Fragments arranged by size:
1861 1318 677 50
MslI CAynn'nnrTG
Cuts at: 0 973 2818 3387 3574 3906
Size: 973 1845 569 187 332
Fragments arranged by size:
1845 973 569 332 187
MspA1I CmG'CkG
Cuts at: 0 43 271 1564 1903 3839 3906
Size: 43 228 1293 339 1936 67
Fragments arranged by size:
1936 1293 339 228 67 43
NdeI CA'TA_TG
Cuts at: 0 3384 3906
Size: 3384 522
NsiI A_TGCA'T
Cuts at: 0 3384 3906
Size: 3384 522
NspI r_CATG'y
Cuts at: 0 312 345 2548 3906
Size: 312 33 2203 1358
Fragments arranged by size:
2203 1358 312 33
PasI CC'CwG_GG
Cuts at: 0 992 2376 3906
Size: 992 1384 1530
Fragments arranged by size:
1530 1384 992
PflMI CCAn_nnn'nTGG
Cuts at: 0 19 1291 1507 3851 3906
Size: 19 1272 216 2344 55
Fragments arranged by size:
2344 1272 216 55 19
PfoI T'CCnGG_A
Cuts at: 0 1373 1506 3906
Size: 1373 133 2400
Fragments arranged by size:
2400 1373 133
PmlI CAC'GTG
Cuts at: 0 3008 3906
Size: 3008 898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 3906
Size: 592 32 3282
Fragments arranged by size:
3282 592 32
PpuMI rG'GwC_Cy
Cuts at: 0 223 1222 2973 3906
Size: 223 999 1751 933
Fragments arranged by size:
1751 999 933 223
PspXI vC'TCGA_Gb
Cuts at: 0 3765 3906
Size: 3765 141
PstI C_TGCA'G
Cuts at: 0 777 1302 1692 2607 3576 3906
Size: 777 525 390 915 969 330
Fragments arranged by size:
969 915 777 525 390 330
PvuII CAG'CTG
Cuts at: 0 1564 1903 3839 3906
Size: 1564 339 1936 67
Fragments arranged by size:
1936 1564 339 67
SacII CC_GC'GG
Cuts at: 0 44 3906
Size: 44 3862
SapI GCTCTTCn'nnn_
Cuts at: 0 356 425 2870 3906
Size: 356 69 2445 1036
Fragments arranged by size:
2445 1036 356 69
SfcI C'TryA_G
Cuts at: 0 743 773 1298 1688 2603 2643 3572 3906
Size: 743 30 525 390 915 40 929 334
Fragments arranged by size:
929 915 743 525 390 334 40 30
SgrAI Cr'CCGG_yG
Cuts at: 0 1191 3906
Size: 1191 2715
SmlI C'TyrA_G
Cuts at: 0 113 2539 3038 3494 3765 3906
Size: 113 2426 499 456 271 141
Fragments arranged by size:
2426 499 456 271 141 113
SphI G_CATG'C
Cuts at: 0 312 3906
Size: 312 3594
SspI AAT'ATT
Cuts at: 0 2035 3508 3906
Size: 2035 1473 398
Fragments arranged by size:
2035 1473 398
StuI AGG'CCT
Cuts at: 0 122 3906
Size: 122 3784
StyI C'CwwG_G
Cuts at: 0 219 285 596 687 871 1248 3225 3906
Size: 219 66 311 91 184 377 1977 681
Fragments arranged by size:
1977 681 377 311 219 184 91 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1279 3906
Size: 1279 2627
TatI w'GTAC_w
Cuts at: 0 329 631 3338 3530 3906
Size: 329 302 2707 192 376
Fragments arranged by size:
2707 376 329 302 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 918 950 1766 1798 2992 3024 3846 3878
Size: 918 32 816 32 1194 32 822 32
Cuts at: 3878 3906
Size: 28
Fragments arranged by size:
1194 918 822 816 32 32 32 32
28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1312 3906
Size: 1312 2594
XhoI C'TCGA_G
Cuts at: 0 3765 3906
Size: 3765 141
XmnI GAAnn'nnTTC
Cuts at: 0 3482 3906
Size: 3482 424
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApoI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI
MslI MspA1I NdeI NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspXI PstI PvuII SacII SapI
SfcI SgrAI SmlI SphI SspI StuI StyI TaqII
TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI ApaI ApaLI AscI AseI AsiSI
BaeI BbeI BcgI BglI BmgBI BmtI BsaBI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI BstAPI
BstBI BstZ17I CspCI DraIII EagI EcoICRI EcoRV FseI
FspI FspAI HpaI KasI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PciI PmeI PshAI
PsiI PspOMI PsrI PvuI RsrII SacI SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI
SpeI SrfI SwaI Tth111I XbaI XmaI ZraI