Restriction map for
pF1KB1499
(Linear) (Six-base) MAPSORT of: KIBB1499.seq Check: 5601 from: 1 to: 3963
KIBB1499.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2995 3963
Size: 2995 968
AccI GT'mk_AC
Cuts at: 0 1004 2662 3963
Size: 1004 1658 1301
Fragments arranged by size:
1658 1301 1004
Acc65I G'GTAC_C
Cuts at: 0 2418 2850 3722 3963
Size: 2418 432 872 241
Fragments arranged by size:
2418 872 432 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2097 2723 3963
Size: 2097 626 1240
Fragments arranged by size:
2097 1240 626
AfeI AGC'GCT
Cuts at: 0 1048 3963
Size: 1048 2915
AflIII A'CryG_T
Cuts at: 0 3064 3425 3963
Size: 3064 361 538
Fragments arranged by size:
3064 538 361
AleI CACnn'nnGTG
Cuts at: 0 1405 3963
Size: 1405 2558
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2893 3963
Size: 2893 1070
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2900 2932 3963
Size: 2900 32 1031
Fragments arranged by size:
2900 1031 32
AlwNI CAG_nnn'CTG
Cuts at: 0 48 123 453 1384 1543 3161 3963
Size: 48 75 330 931 159 1618 802
Fragments arranged by size:
1618 931 802 330 159 75 48
ApaI G_GGCC'C
Cuts at: 0 9 799 982 1154 1746 1777 3963
Size: 9 790 183 172 592 31 2186
Fragments arranged by size:
2186 790 592 183 172 31 9
ApaLI G'TGCA_C
Cuts at: 0 830 3019 3963
Size: 830 2189 944
Fragments arranged by size:
2189 944 830
ApoI r'AATT_y
Cuts at: 0 2436 2525 3954 3963
Size: 2436 89 1429 9
Fragments arranged by size:
2436 1429 89 9
AseI AT'TA_AT
Cuts at: 0 3404 3963
Size: 3404 559
AvaI C'yCGr_G
Cuts at: 0 209 500 764 915 987 2259 2931 3963
Size: 209 291 264 151 72 1272 672 1032
Fragments arranged by size:
1272 1032 672 291 264 209 151 72
AvrII C'CTAG_G
Cuts at: 0 1429 2766 3963
Size: 1429 1337 1197
Fragments arranged by size:
1429 1337 1197
BamHI G'GATC_C
Cuts at: 0 2539 3963
Size: 2539 1424
BanI G'GyrC_C
Cuts at: 0 353 456 850 2418 2850 3079 3175 3682
Size: 353 103 394 1568 432 229 96 507
Cuts at: 3682 3722 3963
Size: 40 241
Fragments arranged by size:
1568 507 432 394 353 241 229 103
96 40
BanII G_rGCy'C
Cuts at: 0 9 110 799 982 1074 1154 1175 1618
Size: 9 101 689 183 92 80 21 443
Cuts at: 1618 1681 1691 1746 1777 2404 2581 2674 2932
Size: 63 10 55 31 627 177 93 258
Cuts at: 2932 3963
Size: 1031
Fragments arranged by size:
1031 689 627 443 258 183 177 101
93 92 80 63 55 31 21 10
9
BbeI G_GCGC'C
Cuts at: 0 357 3963
Size: 357 3606
BbsI GAAGACnn'nnnn_
Cuts at: 0 777 2271 2403 3043 3963
Size: 777 1494 132 640 920
Fragments arranged by size:
1494 920 777 640 132
BbvCI CC'TCA_GC
Cuts at: 0 527 1285 3963
Size: 527 758 2678
Fragments arranged by size:
2678 758 527
BciVI GTATCCnnnnn_n'
Cuts at: 0 662 2266 2589 3963
Size: 662 1604 323 1374
Fragments arranged by size:
1604 1374 662 323
BclI T'GATC_A
Cuts at: 0 3102 3963
Size: 3102 861
BglI GCCn_nnn'nGGC
Cuts at: 0 538 859 966 3359 3691 3963
Size: 538 321 107 2393 332 272
Fragments arranged by size:
2393 538 332 321 272 107
BglII A'GATC_T
Cuts at: 0 1884 3963
Size: 1884 2079
BlpI GC'TnA_GC
Cuts at: 0 555 1342 2156 3963
Size: 555 787 814 1807
Fragments arranged by size:
1807 814 787 555
Bme1580I G_kGCm'C
Cuts at: 0 9 76 461 799 834 982 1154 1746
Size: 9 67 385 338 35 148 172 592
Cuts at: 1746 1777 3023 3634 3687 3963
Size: 31 1246 611 53 276
Fragments arranged by size:
1246 611 592 385 338 276 172 148
67 53 35 31 9
BmgBI CAC'GTC
Cuts at: 0 1085 3963
Size: 1085 2878
BmrI ACTGGGnnnn_n'
Cuts at: 0 657 1150 1337 1772 2132 2504 2677 3945
Size: 657 493 187 435 360 372 173 1268
Cuts at: 3945 3963
Size: 18
Fragments arranged by size:
1268 657 493 435 372 360 187 173
18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2841 3039 3687 3963
Size: 2841 198 648 276
Fragments arranged by size:
2841 648 276 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 1201 1673 1774 2148 2154 2426 2647
Size: 1189 12 472 101 374 6 272 221
Cuts at: 2647 2850 2932 3963
Size: 203 82 1031
Fragments arranged by size:
1189 1031 472 374 272 221 203 101
82 12 6
Bpu10I CC'TnA_GC
Cuts at: 0 527 1285 2141 3963
Size: 527 758 856 1822
Fragments arranged by size:
1822 856 758 527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2595 2661 3963
Size: 2595 66 1302
Fragments arranged by size:
2595 1302 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 1091 1099 2014 2122 2710 3963
Size: 1091 8 915 108 588 1253
Fragments arranged by size:
1253 1091 915 588 108 8
BsaAI yAC'GTr
Cuts at: 0 792 3428 3963
Size: 792 2636 535
Fragments arranged by size:
2636 792 535
BsaHI Gr'CG_yC
Cuts at: 0 354 958 1055 2611 3963
Size: 354 604 97 1556 1352
Fragments arranged by size:
1556 1352 604 354 97
BsaWI w'CCGG_w
Cuts at: 0 1019 1485 3314 3384 3963
Size: 1019 466 1829 70 579
Fragments arranged by size:
1829 1019 579 466 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1123 1153 1552 1582 2152 2182 2726 2756
Size: 1123 30 399 30 570 30 544 30
Cuts at: 2756 2919 2949 3963
Size: 163 30 1014
Fragments arranged by size:
1123 1014 570 544 399 163 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1198 1273 2664 2968 2986 3028 3963
Size: 1198 75 1391 304 18 42 935
Fragments arranged by size:
1391 1198 935 304 75 42 18
BseYI C'CCAG_C
Cuts at: 0 61 123 771 981 1357 1523 1720 1840
Size: 61 62 648 210 376 166 197 120
Cuts at: 1840 3220 3746 3963
Size: 1380 526 217
Fragments arranged by size:
1380 648 526 376 217 210 197 166
120 62 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 862 2353 3795 3963
Size: 160 702 1491 1442 168
Fragments arranged by size:
1491 1442 702 168 160
BsiEI CG_ry'CG
Cuts at: 0 690 3963
Size: 690 3273
BsiHKAI G_wGCw'C
Cuts at: 0 834 1074 1681 2162 3023 3641 3963
Size: 834 240 607 481 861 618 322
Fragments arranged by size:
861 834 618 607 481 322 240
BsmI GAATG_Cn'
Cuts at: 0 1861 3006 3672 3963
Size: 1861 1145 666 291
Fragments arranged by size:
1861 1145 666 291
BsmBI CGTCTCn'nnnn_
Cuts at: 0 553 3963
Size: 553 3410
Bsp1286I G_dGCh'C
Cuts at: 0 9 76 110 461 799 834 982 1074
Size: 9 67 34 351 338 35 148 92
Cuts at: 1074 1154 1175 1618 1681 1691 1746 1777 2162
Size: 80 21 443 63 10 55 31 385
Cuts at: 2162 2404 2581 2674 2932 3023 3634 3641 3687
Size: 242 177 93 258 91 611 7 46
Cuts at: 3687 3963
Size: 276
Fragments arranged by size:
611 443 385 351 338 276 258 242
177 148 93 92 91 80 67 63
55 46 35 34 31 21 10 9
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2995 3963
Size: 2995 968
BsrBI CCG'CTC
Cuts at: 0 3356 3963
Size: 3356 607
BsrDI GCAATG_nn'
Cuts at: 0 563 3738 3786 3963
Size: 563 3175 48 177
Fragments arranged by size:
3175 563 177 48
BsrFI r'CCGG_y
Cuts at: 0 820 965 1024 2502 2775 3963
Size: 820 145 59 1478 273 1188
Fragments arranged by size:
1478 1188 820 273 145 59
BsrGI T'GTAC_A
Cuts at: 0 2354 3963
Size: 2354 1609
BssHII G'CGCG_C
Cuts at: 0 824 3963
Size: 824 3139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 48 1384 3963
Size: 48 1336 2579
Fragments arranged by size:
2579 1336 48
BstBI TT'CG_AA
Cuts at: 0 2906 3963
Size: 2906 1057
BstEII G'GTnAC_C
Cuts at: 0 1197 3963
Size: 1197 2766
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 1641 1691 3963
Size: 282 1359 50 2272
Fragments arranged by size:
2272 1359 282 50
BstYI r'GATC_y
Cuts at: 0 1884 2539 3027 3963
Size: 1884 655 488 936
Fragments arranged by size:
1884 936 655 488
Bsu36I CC'TnA_GG
Cuts at: 0 2248 2582 2835 3963
Size: 2248 334 253 1128
Fragments arranged by size:
2248 1128 334 253
BtgI C'CryG_G
Cuts at: 0 193 1574 1709 1994 2176 2483 2854 3267
Size: 193 1381 135 285 182 307 371 413
Cuts at: 3267 3963
Size: 696
Fragments arranged by size:
1381 696 413 371 307 285 193 182
135
BtsI GCAGTG_nn'
Cuts at: 0 334 1382 1553 1605 3665 3963
Size: 334 1048 171 52 2060 298
Fragments arranged by size:
2060 1048 334 298 171 52
DraIII CAC_nnn'GTG
Cuts at: 0 1981 3756 3963
Size: 1981 1775 207
Fragments arranged by size:
1981 1775 207
EaeI y'GGCC_r
Cuts at: 0 196 284 308 818 926 1571 1602 1871
Size: 196 88 24 510 108 645 31 269
Cuts at: 1871 1898 3360 3758 3963
Size: 27 1462 398 205
Fragments arranged by size:
1462 645 510 398 269 205 196 108
88 31 27 24
EarI CTCTTCn'nnn_
Cuts at: 0 1184 3963
Size: 1184 2779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 3963
Size: 169 3794
EcoICRI GAG'CTC
Cuts at: 0 1072 1679 3963
Size: 1072 607 2284
Fragments arranged by size:
2284 1072 607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1189 1201 1673 1774 2097 2148 2154 2426
Size: 1189 12 472 101 323 51 6 272
Cuts at: 2426 2647 2723 2850 2932 3963
Size: 221 76 127 82 1031
Fragments arranged by size:
1189 1031 472 323 272 221 127 101
82 76 51 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1183 2629 3963
Size: 1183 1446 1334
Fragments arranged by size:
1446 1334 1183
EcoO109I rG'GnC_Cy
Cuts at: 0 5 6 450 897 978 1150 1151 1302
Size: 5 1 444 447 81 172 1 151
Cuts at: 1302 1742 1743 1773 1774 2337 2467 2859 2916
Size: 440 1 30 1 563 130 392 57
Cuts at: 2916 2990 3797 3963
Size: 74 807 166
Fragments arranged by size:
807 563 447 444 440 392 172 166
151 130 81 74 57 30 5 1
1 1 1
EcoRV GAT'ATC
Cuts at: 0 84 3963
Size: 84 3879
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2318 3963
Size: 2318 1645
FspI TGC'GCA
Cuts at: 0 997 3963
Size: 997 2966
FspAI rTGC'GCAy
Cuts at: 0 997 3963
Size: 997 2966
HaeII r_GCGC'y
Cuts at: 0 357 972 1050 1446 1467 1523 3582 3963
Size: 357 615 78 396 21 56 2059 381
Fragments arranged by size:
2059 615 396 381 357 78 56 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1096 1123 1128 1155 3963
Size: 1096 27 5 27 2808
Fragments arranged by size:
2808 1096 27 27 5
HincII GTy'rAC
Cuts at: 0 2148 3963
Size: 2148 1815
KasI G'GCGC_C
Cuts at: 0 353 3963
Size: 353 3610
KpnI G_GTAC'C
Cuts at: 0 2422 2854 3726 3963
Size: 2422 432 872 237
Fragments arranged by size:
2422 872 432 237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2625 3963
Size: 2625 1338
MscI TGG'CCA
Cuts at: 0 286 310 1573 1604 1873 1900 3760 3963
Size: 286 24 1263 31 269 27 1860 203
Fragments arranged by size:
1860 1263 286 269 203 31 27 24
MslI CAynn'nnrTG
Cuts at: 0 280 1405 1588 3963
Size: 280 1125 183 2375
Fragments arranged by size:
2375 1125 280 183
MspA1I CmG'CkG
Cuts at: 0 416 876 3269 3963
Size: 416 460 2393 694
Fragments arranged by size:
2393 694 460 416
NaeI GCC'GGC
Cuts at: 0 822 967 2504 3963
Size: 822 145 1537 1459
Fragments arranged by size:
1537 1459 822 145
NarI GG'CG_CC
Cuts at: 0 354 3963
Size: 354 3609
NcoI C'CATG_G
Cuts at: 0 1574 1709 2176 2483 3963
Size: 1574 135 467 307 1480
Fragments arranged by size:
1574 1480 467 307 135
NgoMIV G'CCGG_C
Cuts at: 0 820 965 2502 3963
Size: 820 145 1537 1461
Fragments arranged by size:
1537 1461 820 145
NspI r_CATG'y
Cuts at: 0 117 3068 3963
Size: 117 2951 895
Fragments arranged by size:
2951 895 117
PasI CC'CwG_GG
Cuts at: 0 1297 1619 1853 2102 2394 2721 2889 3963
Size: 1297 322 234 249 292 327 168 1074
Fragments arranged by size:
1297 1074 327 322 292 249 234 168
PciI A'CATG_T
Cuts at: 0 3064 3963
Size: 3064 899
PflMI CCAn_nnn'nTGG
Cuts at: 0 317 1117 1922 2177 3963
Size: 317 800 805 255 1786
Fragments arranged by size:
1786 805 800 317 255
PfoI T'CCnGG_A
Cuts at: 0 1211 1751 3963
Size: 1211 540 2212
Fragments arranged by size:
2212 1211 540
PmlI CAC'GTG
Cuts at: 0 3428 3963
Size: 3428 535
PpuMI rG'GwC_Cy
Cuts at: 0 450 1302 2337 2467 2859 2916 2990 3963
Size: 450 852 1035 130 392 57 74 973
Fragments arranged by size:
1035 973 852 450 392 130 74 57
PshAI GACnn'nnGTC
Cuts at: 0 330 3373 3963
Size: 330 3043 590
Fragments arranged by size:
3043 590 330
PspOMI G'GGCC_C
Cuts at: 0 5 795 978 1150 1742 1773 3963
Size: 5 790 183 172 592 31 2190
Fragments arranged by size:
2190 790 592 183 172 31 5
PstI C_TGCA'G
Cuts at: 0 44 191 1389 1548 2019 2805 3157 3963
Size: 44 147 1198 159 471 786 352 806
Fragments arranged by size:
1198 806 786 471 352 159 147 44
PvuII CAG'CTG
Cuts at: 0 876 3963
Size: 876 3087
RsrII CG'GwC_CG
Cuts at: 0 1022 3963
Size: 1022 2941
SacI G_AGCT'C
Cuts at: 0 1074 1681 3963
Size: 1074 607 2282
Fragments arranged by size:
2282 1074 607
SacII CC_GC'GG
Cuts at: 0 3270 3963
Size: 3270 693
SanDI GG'GwC_CC
Cuts at: 0 1302 2859 2990 3963
Size: 1302 1557 131 973
Fragments arranged by size:
1557 1302 973 131
SexAI A'CCwGG_T
Cuts at: 0 452 3963
Size: 452 3511
SfcI C'TryA_G
Cuts at: 0 40 187 583 1385 1544 2015 2204 2321
Size: 40 147 396 802 159 471 189 117
Cuts at: 2321 2456 2801 3093 3153 3963
Size: 135 345 292 60 810
Fragments arranged by size:
810 802 471 396 345 292 189 159
147 135 117 60 40
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 538 3963
Size: 538 3425
SfoI GGC'GCC
Cuts at: 0 355 3963
Size: 355 3608
SgrAI Cr'CCGG_yG
Cuts at: 0 965 3963
Size: 965 2998
SmaI CCC'GGG
Cuts at: 0 502 766 917 989 2261 2933 3963
Size: 502 264 151 72 1272 672 1030
Fragments arranged by size:
1272 1030 672 502 264 151 72
SmlI C'TyrA_G
Cuts at: 0 2574 2676 3963
Size: 2574 102 1287
Fragments arranged by size:
2574 1287 102
StuI AGG'CCT
Cuts at: 0 541 3963
Size: 541 3422
StyI C'CwwG_G
Cuts at: 0 23 236 287 461 1429 1574 1709 1817
Size: 23 213 51 174 968 145 135 108
Cuts at: 1817 2024 2176 2446 2483 2766 3963
Size: 207 152 270 37 283 1197
Fragments arranged by size:
1197 968 283 270 213 207 174 152
145 135 108 51 37 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 676 1734 2708 3938 3963
Size: 676 1058 974 1230 25
Fragments arranged by size:
1230 1058 974 676 25
TatI w'GTAC_w
Cuts at: 0 2354 3698 3963
Size: 2354 1344 265
Fragments arranged by size:
2354 1344 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2152 2184 2459 2463 2491 2495 2531 2563
Size: 2152 32 275 4 28 4 36 32
Cuts at: 2563 2726 2758 2919 2951 3874 3906 3963
Size: 163 32 161 32 923 32 57
Fragments arranged by size:
2152 923 275 163 161 57 36 32
32 32 32 32 28 4 4
Tth111I GACn'n_nGTC
Cuts at: 0 249 295 3963
Size: 249 46 3668
Fragments arranged by size:
3668 249 46
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 670 1805 2664 3932 3963
Size: 375 295 1135 859 1268 31
Fragments arranged by size:
1268 1135 859 375 295 31
XmaI C'CCGG_G
Cuts at: 0 500 764 915 987 2259 2931 3963
Size: 500 264 151 72 1272 672 1032
Fragments arranged by size:
1272 1032 672 500 264 151 72
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII RsrII SacI SacII SanDI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AscI AsiSI BaeI
BcgI BfrBI BmtI BsaBI BsiWI BspEI BspHI BssSI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRI
FseI HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PmeI PpiI PsiI PspXI PsrI
PvuI SalI SapI SbfI ScaI SgfI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI XmnI ZraI