(Linear) (Six-base) MAPSORT of: KIBB1496.seq  Check: 5640  from: 1  to: 831



KIBB1496.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     556     831 
   Size:        556     275
 
AlwNI CAG_nnn'CTG

Cuts at:      0     599     831 
   Size:        599     232
 
ApaI G_GGCC'C

Cuts at:      0      42     252     831 
   Size:         42     210     579

  Fragments arranged by size: 

                 579     210      42
 
BanI G'GyrC_C

Cuts at:      0     820     831 
   Size:        820      11
 
BanII G_rGCy'C

Cuts at:      0      42     252     831 
   Size:         42     210     579

  Fragments arranged by size: 

                 579     210      42
 
BbvCI CC'TCA_GC

Cuts at:      0     263     831 
   Size:        263     568
 
BglII A'GATC_T

Cuts at:      0     296     831 
   Size:        296     535
 
Bme1580I G_kGCm'C

Cuts at:      0      42     252     831 
   Size:         42     210     579

  Fragments arranged by size: 

                 579     210      42
 
Bpu10I CC'TnA_GC

Cuts at:      0     263     337     831 
   Size:        263      74     494

  Fragments arranged by size: 

                 494     263      74
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     136     831 
   Size:        136     695
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     397     427     831 
   Size:        397      30     404

  Fragments arranged by size: 

                 404     397      30
 
BseYI C'CCAG_C

Cuts at:      0     568     831 
   Size:        568     263
 
BsmI GAATG_Cn'

Cuts at:      0     642     831 
   Size:        642     189
 
Bsp1286I G_dGCh'C

Cuts at:      0      42     252     831 
   Size:         42     210     579

  Fragments arranged by size: 

                 579     210      42
 
BspHI T'CATG_A

Cuts at:      0     770     831 
   Size:        770      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     556     831 
   Size:        556     275
 
BsrBI CCG'CTC

Cuts at:      0     408     831 
   Size:        408     423
 
BsrFI r'CCGG_y

Cuts at:      0     280     831 
   Size:        280     551
 
BsrGI T'GTAC_A

Cuts at:      0     521     831 
   Size:        521     310
 
BstYI r'GATC_y

Cuts at:      0     296     831 
   Size:        296     535
 
BtgI C'CryG_G

Cuts at:      0     631     831 
   Size:        631     200
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     639     674     831 
   Size:        639      35     157

  Fragments arranged by size: 

                 639     157      35
 
EaeI y'GGCC_r

Cuts at:      0      59     831 
   Size:         59     772
 
EcoO109I rG'GnC_Cy

Cuts at:      0      38      39     248     249     333     831 
   Size:         38       1     209       1      84     498

  Fragments arranged by size: 

                 498     209      84      38       1       1
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     689     831 
   Size:        689     142
 
MscI TGG'CCA

Cuts at:      0      61     831 
   Size:         61     770
 
MslI CAynn'nnrTG

Cuts at:      0     187     769     831 
   Size:        187     582      62

  Fragments arranged by size: 

                 582     187      62
 
MspA1I CmG'CkG

Cuts at:      0     691     831 
   Size:        691     140
 
NcoI C'CATG_G

Cuts at:      0     631     831 
   Size:        631     200
 
PspOMI G'GGCC_C

Cuts at:      0      38     248     831 
   Size:         38     210     583

  Fragments arranged by size: 

                 583     210      38
 
PstI C_TGCA'G

Cuts at:      0     744     831 
   Size:        744      87
 
PvuII CAG'CTG

Cuts at:      0     691     831 
   Size:        691     140
 
SfcI C'TryA_G

Cuts at:      0     740     831 
   Size:        740      91
 
SgrAI Cr'CCGG_yG

Cuts at:      0     280     831 
   Size:        280     551
 
StyI C'CwwG_G

Cuts at:      0     631     831 
   Size:        631     200
 
TatI w'GTAC_w

Cuts at:      0     521     831 
   Size:        521     310
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     397     429     831 
   Size:        397      32     402

  Fragments arranged by size: 

                 402     397      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     640     831 
   Size:        640     191
 

 Enzymes that do cut:

     AarI    AlwNI     ApaI     BanI    BanII    BbvCI    BglII Bme1580I
   Bpu10I     BsaI    BsaXI    BseYI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrFI    BsrGI    BstYI     BtgI    CspCI     EaeI EcoO109I
     MmeI     MscI     MslI   MspA1I     NcoI   PspOMI     PstI    PvuII
     SfcI    SgrAI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrDI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI