Restriction map for
pF1KSDB1489
(Linear) (Six-base) MAPSORT of: KIBB1489.seq Check: 4697 from: 1 to: 4554
KIBB1489.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 216 747 1638 1845 1886 4554
Size: 216 531 891 207 41 2668
Fragments arranged by size:
2668 891 531 216 207 41
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3995 4554
Size: 3995 559
AfeI AGC'GCT
Cuts at: 0 1789 2908 4554
Size: 1789 1119 1646
Fragments arranged by size:
1789 1646 1119
AflIII A'CryG_T
Cuts at: 0 1267 3773 4554
Size: 1267 2506 781
Fragments arranged by size:
2506 1267 781
AleI CACnn'nnGTG
Cuts at: 0 1762 3397 4554
Size: 1762 1635 1157
Fragments arranged by size:
1762 1635 1157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2643 2653 4554
Size: 2643 10 1901
Fragments arranged by size:
2643 1901 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2160 2192 4554
Size: 2160 32 2362
Fragments arranged by size:
2362 2160 32
AlwNI CAG_nnn'CTG
Cuts at: 0 80 608 1444 1489 2221 2332 2487 2529
Size: 80 528 836 45 732 111 155 42
Cuts at: 2529 2999 3516 3838 4554
Size: 470 517 322 716
Fragments arranged by size:
836 732 716 528 517 470 322 155
111 80 45 42
ApaI G_GGCC'C
Cuts at: 0 730 1087 1150 2164 3046 3664 3927 4027
Size: 730 357 63 1014 882 618 263 100
Cuts at: 4027 4554
Size: 527
Fragments arranged by size:
1014 882 730 618 527 357 263 100
63
ApaLI G'TGCA_C
Cuts at: 0 886 1873 2784 3400 4365 4554
Size: 886 987 911 616 965 189
Fragments arranged by size:
987 965 911 886 616 189
AvaI C'yCGr_G
Cuts at: 0 96 150 304 721 759 1187 1204 2315
Size: 96 54 154 417 38 428 17 1111
Cuts at: 2315 2762 4008 4105 4338 4475 4554
Size: 447 1246 97 233 137 79
Fragments arranged by size:
1246 1111 447 428 417 233 154 137
97 96 79 54 38 17
BamHI G'GATC_C
Cuts at: 0 2409 4554
Size: 2409 2145
BanI G'GyrC_C
Cuts at: 0 483 717 1171 1339 2172 3161 3708 4415
Size: 483 234 454 168 833 989 547 707
Cuts at: 4415 4554
Size: 139
Fragments arranged by size:
989 833 707 547 483 454 234 168
139
BanII G_rGCy'C
Cuts at: 0 461 730 1087 1150 1942 1988 2164 2366
Size: 461 269 357 63 792 46 176 202
Cuts at: 2366 2384 2590 3046 3268 3644 3664 3927 4027
Size: 18 206 456 222 376 20 263 100
Cuts at: 4027 4171 4262 4459 4517 4554
Size: 144 91 197 58 37
Fragments arranged by size:
792 461 456 376 357 269 263 222
206 202 197 176 144 100 91 63
58 46 37 20 18
BbeI G_GCGC'C
Cuts at: 0 487 4419 4554
Size: 487 3932 135
Fragments arranged by size:
3932 487 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 3084 3357 3482 4554
Size: 3084 273 125 1072
Fragments arranged by size:
3084 1072 273 125
BbvCI CC'TCA_GC
Cuts at: 0 743 3676 4554
Size: 743 2933 878
Fragments arranged by size:
2933 878 743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1185 1219 2877 2911 4554
Size: 1185 34 1658 34 1643
Fragments arranged by size:
1658 1643 1185 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2974 4554
Size: 2974 1580
BclI T'GATC_A
Cuts at: 0 1967 4554
Size: 1967 2587
BglI GCCn_nnn'nGGC
Cuts at: 0 604 2036 2292 3316 3659 3668 3922 4554
Size: 604 1432 256 1024 343 9 254 632
Fragments arranged by size:
1432 1024 632 604 343 256 254 9
BlpI GC'TnA_GC
Cuts at: 0 455 620 1850 2095 2360 2770 3192 3223
Size: 455 165 1230 245 265 410 422 31
Cuts at: 3223 3228 4554
Size: 5 1326
Fragments arranged by size:
1326 1230 455 422 410 265 245 165
31 5
Bme1580I G_kGCm'C
Cuts at: 0 722 730 890 977 1087 1150 1176 1213
Size: 722 8 160 87 110 63 26 37
Cuts at: 1213 1318 1342 1471 1518 1769 1877 2164 2788
Size: 105 24 129 47 251 108 287 624
Cuts at: 2788 3046 3166 3404 3607 3664 3713 3927 4027
Size: 258 120 238 203 57 49 214 100
Cuts at: 4027 4106 4148 4369 4554
Size: 79 42 221 185
Fragments arranged by size:
722 624 287 258 251 238 221 214
203 185 160 129 120 110 108 105
100 87 79 63 57 49 47 42
37 26 24 8
BmgBI CAC'GTC
Cuts at: 0 1097 1268 1427 4554
Size: 1097 171 159 3127
Fragments arranged by size:
3127 1097 171 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 68 831 1542 2058 2472 4462 4554
Size: 68 763 711 516 414 1990 92
Fragments arranged by size:
1990 763 711 516 414 92 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2007 3852 4554
Size: 2007 1845 702
Fragments arranged by size:
2007 1845 702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 594 662 936 2339 2421 2509 2596
Size: 190 404 68 274 1403 82 88 87
Cuts at: 2596 3034 3282 4027 4195 4449 4554
Size: 438 248 745 168 254 105
Fragments arranged by size:
1403 745 438 404 274 254 248 190
168 105 88 87 82 68
Bpu10I CC'TnA_GC
Cuts at: 0 396 743 1010 2454 3676 4554
Size: 396 347 267 1444 1222 878
Fragments arranged by size:
1444 1222 878 396 347 267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1909 4554
Size: 1909 2645
BsaI GGTCTCn'nnnn_
Cuts at: 0 2485 4554
Size: 2485 2069
BsaAI yAC'GTr
Cuts at: 0 1413 1764 3112 4554
Size: 1413 351 1348 1442
Fragments arranged by size:
1442 1413 1348 351
BsaBI GATnn'nnATC
Cuts at: 0 3539 4554
Size: 3539 1015
BsaHI Gr'CG_yC
Cuts at: 0 484 4416 4554
Size: 484 3932 138
Fragments arranged by size:
3932 484 138
BsaWI w'CCGG_w
Cuts at: 0 1342 4527 4554
Size: 1342 3185 27
Fragments arranged by size:
3185 1342 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 660 690 895 925 1337 1367
Size: 214 30 416 30 205 30 412 30
Cuts at: 1367 2160 2190 2337 2367 3631 3661 4309 4339
Size: 793 30 147 30 1264 30 648 30
Cuts at: 4339 4554
Size: 215
Fragments arranged by size:
1264 793 648 416 412 215 214 205
147 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 322 367 370 595 820 916 1363 1498
Size: 322 45 3 225 225 96 447 135
Cuts at: 1498 2094 2197 2814 3172 3652 4554
Size: 596 103 617 358 480 902
Fragments arranged by size:
902 617 596 480 447 358 322 225
225 135 103 96 45 3
BseYI C'CCAG_C
Cuts at: 0 6 729 980 1149 1543 2211 2348 2426
Size: 6 723 251 169 394 668 137 78
Cuts at: 2426 2552 2834 2924 3211 3732 3985 4020 4087
Size: 126 282 90 287 521 253 35 67
Cuts at: 4087 4378 4396 4554
Size: 291 18 158
Fragments arranged by size:
723 668 521 394 291 287 282 253
251 169 158 137 126 90 78 67
35 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 408 457 1155 1215 1419 1804 2639
Size: 400 8 49 698 60 204 385 835
Cuts at: 2639 2791 4477 4554
Size: 152 1686 77
Fragments arranged by size:
1686 835 698 400 385 204 152 77
60 49 8
BsiEI CG_ry'CG
Cuts at: 0 3931 4287 4554
Size: 3931 356 267
Fragments arranged by size:
3931 356 267
BsiHKAI G_wGCw'C
Cuts at: 0 107 566 890 928 1261 1604 1877 1942
Size: 107 459 324 38 333 343 273 65
Cuts at: 1942 2096 2384 2788 3268 3404 4262 4369 4435
Size: 154 288 404 480 136 858 107 66
Cuts at: 4435 4554
Size: 119
Fragments arranged by size:
858 480 459 404 343 333 324 288
273 154 136 119 107 107 66 65
38
BsmI GAATG_Cn'
Cuts at: 0 1248 1482 1973 4239 4554
Size: 1248 234 491 2266 315
Fragments arranged by size:
2266 1248 491 315 234
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1995 4554
Size: 1995 2559
Bsp1286I G_dGCh'C
Cuts at: 0 107 461 566 722 730 890 928 977
Size: 107 354 105 156 8 160 38 49
Cuts at: 977 1087 1150 1176 1213 1261 1318 1342 1471
Size: 110 63 26 37 48 57 24 129
Cuts at: 1471 1518 1604 1769 1877 1942 1988 2096 2164
Size: 47 86 165 108 65 46 108 68
Cuts at: 2164 2366 2384 2590 2788 3046 3166 3268 3404
Size: 202 18 206 198 258 120 102 136
Cuts at: 3404 3607 3644 3664 3713 3927 4027 4106 4148
Size: 203 37 20 49 214 100 79 42
Cuts at: 4148 4171 4262 4369 4435 4459 4517 4554
Size: 23 91 107 66 24 58 37
Fragments arranged by size:
354 258 214 206 203 202 198 165
160 156 136 129 120 110 108 108
107 107 105 102 100 91 86 79
68 66 65 63 58 57 49 49
48 47 46 42 38 37 37 37
26 24 24 23 20 18 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 216 295 747 976 1638 1712 1845
Size: 212 4 79 452 229 662 74 133
Cuts at: 1845 1886 4554
Size: 41 2668
Fragments arranged by size:
2668 662 452 229 212 133 79 74
41 4
BsrBI CCG'CTC
Cuts at: 0 3159 3205 4356 4554
Size: 3159 46 1151 198
Fragments arranged by size:
3159 1151 198 46
BsrDI GCAATG_nn'
Cuts at: 0 777 2415 4554
Size: 777 1638 2139
Fragments arranged by size:
2139 1638 777
BsrFI r'CCGG_y
Cuts at: 0 598 655 4554
Size: 598 57 3899
Fragments arranged by size:
3899 598 57
BssSI C'ACGA_G
Cuts at: 0 2749 3489 3655 4255 4554
Size: 2749 740 166 600 299
Fragments arranged by size:
2749 740 600 299 166
BstAPI GCAn_nnn'nTGC
Cuts at: 0 241 608 885 998 1623 1653 4554
Size: 241 367 277 113 625 30 2901
Fragments arranged by size:
2901 625 367 277 241 113 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 714 3963 4164 4554
Size: 714 3249 201 390
Fragments arranged by size:
3249 714 390 201
BstYI r'GATC_y
Cuts at: 0 2409 3512 3540 4036 4554
Size: 2409 1103 28 496 518
Fragments arranged by size:
2409 1103 518 496 28
Bsu36I CC'TnA_GG
Cuts at: 0 1482 3665 4554
Size: 1482 2183 889
Fragments arranged by size:
2183 1482 889
BtgI C'CryG_G
Cuts at: 0 1237 1310 2405 3248 3713 3998 4304 4554
Size: 1237 73 1095 843 465 285 306 250
Fragments arranged by size:
1237 1095 843 465 306 285 250 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 129 1931 4554
Size: 129 1802 2623
Fragments arranged by size:
2623 1802 129
BtsI GCAGTG_nn'
Cuts at: 0 2627 3392 3402 3688 4554
Size: 2627 765 10 286 866
Fragments arranged by size:
2627 866 765 286 10
DraIII CAC_nnn'GTG
Cuts at: 0 894 1911 4554
Size: 894 1017 2643
Fragments arranged by size:
2643 1017 894
EaeI y'GGCC_r
Cuts at: 0 50 343 588 841 1214 1607 2303 2948
Size: 50 293 245 253 373 393 696 645
Cuts at: 2948 3236 3928 4307 4554
Size: 288 692 379 247
Fragments arranged by size:
696 692 645 393 379 373 293 288
253 247 245 50
EagI C'GGCC_G
Cuts at: 0 3928 4554
Size: 3928 626
EarI CTCTTCn'nnn_
Cuts at: 0 344 809 1366 1714 2080 3349 4554
Size: 344 465 557 348 366 1269 1205
Fragments arranged by size:
1269 1205 557 465 366 348 344
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 340 2895 3734 3882 4554
Size: 340 2555 839 148 672
Fragments arranged by size:
2555 839 672 340 148
EcoICRI GAG'CTC
Cuts at: 0 1940 2382 3266 4260 4554
Size: 1940 442 884 994 294
Fragments arranged by size:
1940 994 884 442 294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 594 662 936 2339 2421 2509 2596
Size: 190 404 68 274 1403 82 88 87
Cuts at: 2596 3034 3282 3995 4027 4195 4449 4554
Size: 438 248 713 32 168 254 105
Fragments arranged by size:
1403 713 438 404 274 254 248 190
168 105 88 87 82 68 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1609 1749 2083 2183 2670 3237 3346 3628
Size: 1609 140 334 100 487 567 109 282
Cuts at: 3628 4554
Size: 926
Fragments arranged by size:
1609 926 567 487 334 282 140 109
100
EcoO109I rG'GnC_Cy
Cuts at: 0 113 129 400 468 605 682 726 911
Size: 113 16 271 68 137 77 44 185
Cuts at: 911 947 1006 1075 1083 1112 1701 2160 2294
Size: 36 59 69 8 29 589 459 134
Cuts at: 2294 2565 3043 3351 3661 3923 3938 4024 4554
Size: 271 478 308 310 262 15 86 530
Fragments arranged by size:
589 530 478 459 310 308 271 271
262 185 137 134 113 86 77 69
68 59 44 36 29 16 15 8
FspI TGC'GCA
Cuts at: 0 234 1657 2887 4554
Size: 234 1423 1230 1667
Fragments arranged by size:
1667 1423 1230 234
FspAI rTGC'GCAy
Cuts at: 0 234 2887 4554
Size: 234 2653 1667
Fragments arranged by size:
2653 1667 234
HaeII r_GCGC'y
Cuts at: 0 487 1290 1791 2205 2638 2910 3295 4094
Size: 487 803 501 414 433 272 385 799
Cuts at: 4094 4395 4419 4554
Size: 301 24 135
Fragments arranged by size:
803 799 501 487 433 414 385 301
272 135 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 895 927 1627 1659 1973 2005
Size: 742 32 121 32 700 32 314 32
Cuts at: 2005 2215 2247 2394 2426 2437 2469 3106 3138
Size: 210 32 147 32 11 32 637 32
Cuts at: 3138 3631 3663 4119 4151 4554
Size: 493 32 456 32 403
Fragments arranged by size:
742 700 637 493 456 403 314 210
147 121 32 32 32 32 32 32
32 32 32 32 11
HincII GTy'rAC
Cuts at: 0 279 3576 4554
Size: 279 3297 978
Fragments arranged by size:
3297 978 279
KasI G'GCGC_C
Cuts at: 0 483 4415 4554
Size: 483 3932 139
Fragments arranged by size:
3932 483 139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 2759 3134 3149 4092 4554
Size: 359 2400 375 15 943 462
Fragments arranged by size:
2400 943 462 375 359 15
MscI TGG'CCA
Cuts at: 0 52 1609 4554
Size: 52 1557 2945
Fragments arranged by size:
2945 1557 52
MslI CAynn'nnrTG
Cuts at: 0 1762 2374 3301 3397 4162 4554
Size: 1762 612 927 96 765 392
Fragments arranged by size:
1762 927 765 612 392 96
MspA1I CmG'CkG
Cuts at: 0 373 392 441 490 572 635 935 1441
Size: 373 19 49 49 82 63 300 506
Cuts at: 1441 1502 1996 2301 2359 2426 2513 3507 3801
Size: 61 494 305 58 67 87 994 294
Cuts at: 3801 4554
Size: 753
Fragments arranged by size:
994 753 506 494 373 305 300 294
87 82 67 63 61 58 49 49
19
NaeI GCC'GGC
Cuts at: 0 600 657 4554
Size: 600 57 3897
Fragments arranged by size:
3897 600 57
NarI GG'CG_CC
Cuts at: 0 484 4416 4554
Size: 484 3932 138
Fragments arranged by size:
3932 484 138
NcoI C'CATG_G
Cuts at: 0 1237 3248 3713 4554
Size: 1237 2011 465 841
Fragments arranged by size:
2011 1237 841 465
NgoMIV G'CCGG_C
Cuts at: 0 598 655 4554
Size: 598 57 3899
Fragments arranged by size:
3899 598 57
NspI r_CATG'y
Cuts at: 0 1166 1302 1626 1644 3075 3777 4554
Size: 1166 136 324 18 1431 702 777
Fragments arranged by size:
1431 1166 777 702 324 136 18
PasI CC'CwG_GG
Cuts at: 0 1079 2110 3167 3920 4384 4554
Size: 1079 1031 1057 753 464 170
Fragments arranged by size:
1079 1057 1031 753 464 170
PciI A'CATG_T
Cuts at: 0 3773 4554
Size: 3773 781
PflMI CCAn_nnn'nTGG
Cuts at: 0 273 1524 2487 3432 3694 4531 4554
Size: 273 1251 963 945 262 837 23
Fragments arranged by size:
1251 963 945 837 273 262 23
PfoI T'CCnGG_A
Cuts at: 0 160 913 949 3425 4554
Size: 160 753 36 2476 1129
Fragments arranged by size:
2476 1129 753 160 36
PmlI CAC'GTG
Cuts at: 0 3112 4554
Size: 3112 1442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2160 2192 4554
Size: 2160 32 2362
Fragments arranged by size:
2362 2160 32
PpuMI rG'GwC_Cy
Cuts at: 0 129 911 947 1006 1075 1112 1701 2565
Size: 129 782 36 59 69 37 589 864
Cuts at: 2565 3938 4554
Size: 1373 616
Fragments arranged by size:
1373 864 782 616 589 129 69 59
37 36
PspOMI G'GGCC_C
Cuts at: 0 726 1083 1146 2160 3042 3660 3923 4023
Size: 726 357 63 1014 882 618 263 100
Cuts at: 4023 4554
Size: 531
Fragments arranged by size:
1014 882 726 618 531 357 263 100
63
PspXI vC'TCGA_Gb
Cuts at: 0 1204 4554
Size: 1204 3350
PstI C_TGCA'G
Cuts at: 0 128 634 855 1019 1033 1667 3051 3512
Size: 128 506 221 164 14 634 1384 461
Cuts at: 3512 3884 4554
Size: 372 670
Fragments arranged by size:
1384 670 634 506 461 372 221 164
128 14
PvuII CAG'CTG
Cuts at: 0 441 635 935 1441 1996 2359 2426 3507
Size: 441 194 300 506 555 363 67 1081
Cuts at: 3507 3801 4554
Size: 294 753
Fragments arranged by size:
1081 753 555 506 441 363 300 294
194 67
SacI G_AGCT'C
Cuts at: 0 1942 2384 3268 4262 4554
Size: 1942 442 884 994 292
Fragments arranged by size:
1942 994 884 442 292
SanDI GG'GwC_CC
Cuts at: 0 947 1075 1112 3938 4554
Size: 947 128 37 2826 616
Fragments arranged by size:
2826 947 616 128 37
SapI GCTCTTCn'nnn_
Cuts at: 0 1714 4554
Size: 1714 2840
SbfI CC_TGCA'GG
Cuts at: 0 1667 4554
Size: 1667 2887
ScaI AGT'ACT
Cuts at: 0 193 4554
Size: 193 4361
SexAI A'CCwGG_T
Cuts at: 0 272 1907 4554
Size: 272 1635 2647
Fragments arranged by size:
2647 1635 272
SfcI C'TryA_G
Cuts at: 0 124 630 851 1015 1029 1663 1692 3047
Size: 124 506 221 164 14 634 29 1355
Cuts at: 3047 3508 3880 4554
Size: 461 372 674
Fragments arranged by size:
1355 674 634 506 461 372 221 164
124 29 14
SfoI GGC'GCC
Cuts at: 0 485 4417 4554
Size: 485 3932 137
Fragments arranged by size:
3932 485 137
SmaI CCC'GGG
Cuts at: 0 723 1189 2317 2764 4554
Size: 723 466 1128 447 1790
Fragments arranged by size:
1790 1128 723 466 447
SmlI C'TyrA_G
Cuts at: 0 1204 1924 4554
Size: 1204 720 2630
Fragments arranged by size:
2630 1204 720
SphI G_CATG'C
Cuts at: 0 1626 4554
Size: 1626 2928
StuI AGG'CCT
Cuts at: 0 997 1930 2851 4554
Size: 997 933 921 1703
Fragments arranged by size:
1703 997 933 921
StyI C'CwwG_G
Cuts at: 0 1237 1518 1610 2084 2268 2561 3248 3713
Size: 1237 281 92 474 184 293 687 465
Cuts at: 3713 4554
Size: 841
Fragments arranged by size:
1237 841 687 474 465 293 281 184
92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2040 2586 2826 4101 4554
Size: 2040 546 240 1275 453
Fragments arranged by size:
2040 1275 546 453 240
TatI w'GTAC_w
Cuts at: 0 191 2253 2544 4554
Size: 191 2062 291 2010
Fragments arranged by size:
2062 2010 291 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 660 688 692 720 1045 1077
Size: 214 32 414 28 4 28 325 32
Cuts at: 1077 1337 1369 4309 4341 4554
Size: 260 32 2940 32 213
Fragments arranged by size:
2940 414 325 260 214 213 32 32
32 32 28 28 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 553 1244 2298 3246 4051 4554
Size: 553 691 1054 948 805 503
Fragments arranged by size:
1054 948 805 691 553 503
XhoI C'TCGA_G
Cuts at: 0 1204 4554
Size: 1204 3350
XmaI C'CCGG_G
Cuts at: 0 721 1187 2315 2762 4554
Size: 721 466 1128 447 1792
Fragments arranged by size:
1792 1128 721 466 447
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BaeI BfrBI BglII BmtI
BsiWI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I
ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PmeI PshAI PsiI PsrI PvuI
RsrII SacII SalI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XmnI ZraI