Restriction map for
pF1KSDB1483
(Linear) (Six-base) MAPSORT of: KIBB1483.seq Check: 7893 from: 1 to: 3594
KIBB1483.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1545 1966 2292 3594
Size: 1545 421 326 1302
Fragments arranged by size:
1545 1302 421 326
AatII G_ACGT'C
Cuts at: 0 1421 3589 3594
Size: 1421 2168 5
Fragments arranged by size:
2168 1421 5
AccI GT'mk_AC
Cuts at: 0 1169 2744 3594
Size: 1169 1575 850
Fragments arranged by size:
1575 1169 850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1624 2599 3594
Size: 1624 975 995
Fragments arranged by size:
1624 995 975
AflIII A'CryG_T
Cuts at: 0 2618 3594
Size: 2618 976
AleI CACnn'nnGTG
Cuts at: 0 1126 2392 2968 3594
Size: 1126 1266 576 626
Fragments arranged by size:
1266 1126 626 576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1651 2986 3594
Size: 1651 1335 608
Fragments arranged by size:
1651 1335 608
AlwNI CAG_nnn'CTG
Cuts at: 0 2389 3249 3594
Size: 2389 860 345
Fragments arranged by size:
2389 860 345
ApaLI G'TGCA_C
Cuts at: 0 75 1158 3594
Size: 75 1083 2436
Fragments arranged by size:
2436 1083 75
ApoI r'AATT_y
Cuts at: 0 239 571 2494 3379 3594
Size: 239 332 1923 885 215
Fragments arranged by size:
1923 885 332 239 215
AvaI C'yCGr_G
Cuts at: 0 1231 2078 2134 3462 3594
Size: 1231 847 56 1328 132
Fragments arranged by size:
1328 1231 847 132 56
BamHI G'GATC_C
Cuts at: 0 2159 3272 3594
Size: 2159 1113 322
Fragments arranged by size:
2159 1113 322
BanI G'GyrC_C
Cuts at: 0 173 208 1540 2551 2977 3594
Size: 173 35 1332 1011 426 617
Fragments arranged by size:
1332 1011 617 426 173 35
BanII G_rGCy'C
Cuts at: 0 92 802 1241 1622 1853 1871 3089 3191
Size: 92 710 439 381 231 18 1218 102
Cuts at: 3191 3469 3568 3594
Size: 278 99 26
Fragments arranged by size:
1218 710 439 381 278 231 102 99
92 26 18
BbeI G_GCGC'C
Cuts at: 0 177 1544 2555 3594
Size: 177 1367 1011 1039
Fragments arranged by size:
1367 1039 1011 177
BbsI GAAGACnn'nnnn_
Cuts at: 0 1797 3594
Size: 1797 1797
BbvCI CC'TCA_GC
Cuts at: 0 2671 3594
Size: 2671 923
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 382 416 2323 2357 3594
Size: 382 34 1907 34 1237
Fragments arranged by size:
1907 1237 382 34 34
BclI T'GATC_A
Cuts at: 0 1091 1478 3594
Size: 1091 387 2116
Fragments arranged by size:
2116 1091 387
BglI GCCn_nnn'nGGC
Cuts at: 0 62 1236 1586 2138 2404 2632 3594
Size: 62 1174 350 552 266 228 962
Fragments arranged by size:
1174 962 552 350 266 228 62
BglII A'GATC_T
Cuts at: 0 2204 3594
Size: 2204 1390
Bme1580I G_kGCm'C
Cuts at: 0 79 118 211 1162 1232 2980 3594
Size: 79 39 93 951 70 1748 614
Fragments arranged by size:
1748 951 614 93 79 70 39
BmgBI CAC'GTC
Cuts at: 0 315 2361 2394 3201 3594
Size: 315 2046 33 807 393
Fragments arranged by size:
2046 807 393 315 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 1932 3594
Size: 1932 1662
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 111 3594
Size: 111 3483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 352 537 746 2691 3594
Size: 200 152 185 209 1945 903
Fragments arranged by size:
1945 903 209 200 185 152
Bpu10I CC'TnA_GC
Cuts at: 0 801 2671 3594
Size: 801 1870 923
Fragments arranged by size:
1870 923 801
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 934 1744 2332 3115 3124 3436 3594
Size: 685 249 810 588 783 9 312 158
Fragments arranged by size:
810 783 685 588 312 249 158 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1321 2653 3594
Size: 1321 1332 941
Fragments arranged by size:
1332 1321 941
BsaAI yAC'GTr
Cuts at: 0 789 1173 3594
Size: 789 384 2421
Fragments arranged by size:
2421 789 384
BsaBI GATnn'nnATC
Cuts at: 0 2998 3594
Size: 2998 596
BsaHI Gr'CG_yC
Cuts at: 0 174 1418 1541 2552 3586 3594
Size: 174 1244 123 1011 1034 8
Fragments arranged by size:
1244 1034 1011 174 123 8
BsaWI w'CCGG_w
Cuts at: 0 2945 3383 3594
Size: 2945 438 211
Fragments arranged by size:
2945 438 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 43 1432 1462 1636 1666 2473 2503
Size: 13 30 1389 30 174 30 807 30
Cuts at: 2503 3074 3104 3594
Size: 571 30 490
Fragments arranged by size:
1389 807 571 490 174 30 30 30
30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 463 652 1657 2226 3184 3196 3199
Size: 100 363 189 1005 569 958 12 3
Cuts at: 3199 3220 3594
Size: 21 374
Fragments arranged by size:
1005 958 569 374 363 189 100 21
12 3
BseYI C'CCAG_C
Cuts at: 0 1444 1584 1903 2973 3398 3594
Size: 1444 140 319 1070 425 196
Fragments arranged by size:
1444 1070 425 319 196 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1195 2327 2608 2946 3025 3594
Size: 1069 126 1132 281 338 79 569
Fragments arranged by size:
1132 1069 569 338 281 126 79
BsiEI CG_ry'CG
Cuts at: 0 1821 2688 3527 3594
Size: 1821 867 839 67
Fragments arranged by size:
1821 867 839 67
BsiHKAI G_wGCw'C
Cuts at: 0 79 92 1106 1162 1696 1760 3089 3191
Size: 79 13 1014 56 534 64 1329 102
Cuts at: 3191 3594
Size: 403
Fragments arranged by size:
1329 1014 534 403 102 79 64 56
13
BsiWI C'GTAC_G
Cuts at: 0 2681 3594
Size: 2681 913
BsmI GAATG_Cn'
Cuts at: 0 2826 3594
Size: 2826 768
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3577 3594
Size: 3577 17
Bsp1286I G_dGCh'C
Cuts at: 0 79 92 118 211 802 1106 1162 1232
Size: 79 13 26 93 591 304 56 70
Cuts at: 1232 1241 1622 1696 1760 1853 1871 2980 3089
Size: 9 381 74 64 93 18 1109 109
Cuts at: 3089 3191 3469 3568 3594
Size: 102 278 99 26
Fragments arranged by size:
1109 591 381 304 278 109 102 99
93 93 79 74 70 64 56 26
26 18 13 9
BspEI T'CCGG_A
Cuts at: 0 2945 3383 3594
Size: 2945 438 211
Fragments arranged by size:
2945 438 211
BspHI T'CATG_A
Cuts at: 0 2873 3594
Size: 2873 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 436 1545 1966 2292 3594
Size: 436 1109 421 326 1302
Fragments arranged by size:
1302 1109 436 421 326
BsrBI CCG'CTC
Cuts at: 0 95 214 2700 3225 3594
Size: 95 119 2486 525 369
Fragments arranged by size:
2486 525 369 119 95
BsrDI GCAATG_nn'
Cuts at: 0 699 1344 2931 3594
Size: 699 645 1587 663
Fragments arranged by size:
1587 699 663 645
BsrFI r'CCGG_y
Cuts at: 0 170 3594
Size: 170 3424
BsrGI T'GTAC_A
Cuts at: 0 1652 1661 1730 3594
Size: 1652 9 69 1864
Fragments arranged by size:
1864 1652 69 9
BssSI C'ACGA_G
Cuts at: 0 292 1799 3594
Size: 292 1507 1795
Fragments arranged by size:
1795 1507 292
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1880 3594
Size: 1880 1714
BstEII G'GTnAC_C
Cuts at: 0 655 3594
Size: 655 2939
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1263 1467 2973 3594
Size: 1263 204 1506 621
Fragments arranged by size:
1506 1263 621 204
BstYI r'GATC_y
Cuts at: 0 605 1433 2159 2204 2909 3173 3245 3272
Size: 605 828 726 45 705 264 72 27
Cuts at: 3272 3284 3594
Size: 12 310
Fragments arranged by size:
828 726 705 605 310 264 72 45
27 12
BtgI C'CryG_G
Cuts at: 0 57 578 752 932 1144 1331 1466 1853
Size: 57 521 174 180 212 187 135 387
Cuts at: 1853 2372 2654 2913 3594
Size: 519 282 259 681
Fragments arranged by size:
681 521 519 387 282 259 212 187
180 174 135 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1473 1877 2070 2364 3090 3594
Size: 1473 404 193 294 726 504
Fragments arranged by size:
1473 726 504 404 294 193
BtsI GCAGTG_nn'
Cuts at: 0 2462 3037 3594
Size: 2462 575 557
Fragments arranged by size:
2462 575 557
EaeI y'GGCC_r
Cuts at: 0 591 1247 1993 2209 2309 2396 2528 2639
Size: 591 656 746 216 100 87 132 111
Cuts at: 2639 2685 3594
Size: 46 909
Fragments arranged by size:
909 746 656 591 216 132 111 100
87 46
EagI C'GGCC_G
Cuts at: 0 2685 3594
Size: 2685 909
EarI CTCTTCn'nnn_
Cuts at: 0 685 875 881 2890 3555 3594
Size: 685 190 6 2009 665 39
Fragments arranged by size:
2009 685 665 190 39 6
EcoICRI GAG'CTC
Cuts at: 0 90 3087 3189 3594
Size: 90 2997 102 405
Fragments arranged by size:
2997 405 102 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 352 537 746 1624 2599 2691 3594
Size: 200 152 185 209 878 975 92 903
Fragments arranged by size:
975 903 878 209 200 185 152 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 194 1606 3142 3594
Size: 194 1412 1536 452
Fragments arranged by size:
1536 1412 452 194
EcoO109I rG'GnC_Cy
Cuts at: 0 540 762 1983 2001 3594
Size: 540 222 1221 18 1593
Fragments arranged by size:
1593 1221 540 222 18
EcoRI G'AATT_C
Cuts at: 0 3379 3594
Size: 3379 215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1083 3594
Size: 1083 2511
FspI TGC'GCA
Cuts at: 0 745 1627 1681 3594
Size: 745 882 54 1913
Fragments arranged by size:
1913 882 745 54
HaeII r_GCGC'y
Cuts at: 0 177 307 1505 1544 2555 3500 3594
Size: 177 130 1198 39 1011 945 94
Fragments arranged by size:
1198 1011 945 177 130 94 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 700 732 1368 1400 1432 1464
Size: 13 32 655 32 636 32 32 32
Cuts at: 1464 2473 2505 3594
Size: 1009 32 1089
Fragments arranged by size:
1089 1009 655 636 32 32 32 32
32 32 13
HincII GTy'rAC
Cuts at: 0 1134 2520 3594
Size: 1134 1386 1074
Fragments arranged by size:
1386 1134 1074
KasI G'GCGC_C
Cuts at: 0 173 1540 2551 3594
Size: 173 1367 1011 1043
Fragments arranged by size:
1367 1043 1011 173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 3594
Size: 430 3164
MscI TGG'CCA
Cuts at: 0 593 2211 2398 2641 3594
Size: 593 1618 187 243 953
Fragments arranged by size:
1618 953 593 243 187
MslI CAynn'nnrTG
Cuts at: 0 937 1087 1099 1126 1204 1316 1393 1966
Size: 937 150 12 27 78 112 77 573
Cuts at: 1966 2392 2968 3594
Size: 426 576 626
Fragments arranged by size:
937 626 576 573 426 150 112 78
77 27 12
MspA1I CmG'CkG
Cuts at: 0 2028 2298 2807 3029 3045 3594
Size: 2028 270 509 222 16 549
Fragments arranged by size:
2028 549 509 270 222 16
NaeI GCC'GGC
Cuts at: 0 172 3594
Size: 172 3422
NarI GG'CG_CC
Cuts at: 0 174 1541 2552 3594
Size: 174 1367 1011 1042
Fragments arranged by size:
1367 1042 1011 174
NcoI C'CATG_G
Cuts at: 0 57 932 1331 1466 1853 2372 3594
Size: 57 875 399 135 387 519 1222
Fragments arranged by size:
1222 875 519 399 387 135 57
NgoMIV G'CCGG_C
Cuts at: 0 170 3594
Size: 170 3424
NspI r_CATG'y
Cuts at: 0 999 1731 3594
Size: 999 732 1863
Fragments arranged by size:
1863 999 732
PasI CC'CwG_GG
Cuts at: 0 354 411 3594
Size: 354 57 3183
Fragments arranged by size:
3183 354 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 792 3579 3594
Size: 792 2787 15
Fragments arranged by size:
2787 792 15
PfoI T'CCnGG_A
Cuts at: 0 329 2707 3176 3594
Size: 329 2378 469 418
Fragments arranged by size:
2378 469 418 329
PmlI CAC'GTG
Cuts at: 0 789 3594
Size: 789 2805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 2776 2808 3594
Size: 517 32 2227 32 786
Fragments arranged by size:
2227 786 517 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 762 1983 2001 3594
Size: 762 1221 18 1593
Fragments arranged by size:
1593 1221 762 18
PspXI vC'TCGA_Gb
Cuts at: 0 2134 3462 3594
Size: 2134 1328 132
Fragments arranged by size:
2134 1328 132
PstI C_TGCA'G
Cuts at: 0 290 311 548 1025 3044 3594
Size: 290 21 237 477 2019 550
Fragments arranged by size:
2019 550 477 290 237 21
PvuII CAG'CTG
Cuts at: 0 3029 3045 3594
Size: 3029 16 549
Fragments arranged by size:
3029 549 16
RsrII CG'GwC_CG
Cuts at: 0 2043 3594
Size: 2043 1551
SacI G_AGCT'C
Cuts at: 0 92 3089 3191 3594
Size: 92 2997 102 403
Fragments arranged by size:
2997 403 102 92
SapI GCTCTTCn'nnn_
Cuts at: 0 2890 3594
Size: 2890 704
ScaI AGT'ACT
Cuts at: 0 1180 3594
Size: 1180 2414
SexAI A'CCwGG_T
Cuts at: 0 737 3594
Size: 737 2857
SfcI C'TryA_G
Cuts at: 0 286 307 544 1021 3040 3594
Size: 286 21 237 477 2019 554
Fragments arranged by size:
2019 554 477 286 237 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2404 3594
Size: 2404 1190
SfoI GGC'GCC
Cuts at: 0 175 1542 2553 3594
Size: 175 1367 1011 1041
Fragments arranged by size:
1367 1041 1011 175
SmlI C'TyrA_G
Cuts at: 0 700 949 1759 2134 2347 3130 3139 3451
Size: 700 249 810 375 213 783 9 312
Cuts at: 3451 3462 3594
Size: 11 132
Fragments arranged by size:
810 783 700 375 312 249 213 132
11 9
SphI G_CATG'C
Cuts at: 0 999 3594
Size: 999 2595
StuI AGG'CCT
Cuts at: 0 1405 2407 3263 3594
Size: 1405 1002 856 331
Fragments arranged by size:
1405 1002 856 331
StyI C'CwwG_G
Cuts at: 0 57 932 1331 1466 1853 2372 2399 3594
Size: 57 875 399 135 387 519 27 1195
Fragments arranged by size:
1195 875 519 399 387 135 57 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 817 2589 3541 3594
Size: 817 1772 952 53
Fragments arranged by size:
1772 952 817 53
TatI w'GTAC_w
Cuts at: 0 1178 1652 1661 1730 3594
Size: 1178 474 9 69 1864
Fragments arranged by size:
1864 1178 474 69 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 773 805 2113 2145 3594
Size: 773 32 1308 32 1449
Fragments arranged by size:
1449 1308 773 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 649 3576 3594
Size: 351 298 2927 18
Fragments arranged by size:
2927 351 298 18
XhoI C'TCGA_G
Cuts at: 0 2134 3462 3594
Size: 2134 1328 132
Fragments arranged by size:
2134 1328 132
XmnI GAAnn'nnTTC
Cuts at: 0 532 2791 3022 3301 3594
Size: 532 2259 231 279 293
Fragments arranged by size:
2259 532 293 279 231
ZraI GAC'GTC
Cuts at: 0 1419 3587 3594
Size: 1419 2168 7
Fragments arranged by size:
2168 1419 7
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspXI
PstI PvuII RsrII SacI SapI ScaI SexAI SfcI
SfiI SfoI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI
AscI AseI AsiSI AvrII BaeI BciVI BfrBI BlpI
BmtI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EciI EcoRV FseI FspAI HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PshAI PsiI PspOMI PsrI PvuI
SacII SalI SanDI SbfI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XmaI