Restriction map for
pF1KB1393
(Linear) (Six-base) MAPSORT of: KIBB1393.seq Check: 4836 from: 1 to: 2430
KIBB1393.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 129 2430
Size: 129 2301
AccI GT'mk_AC
Cuts at: 0 2354 2430
Size: 2354 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 257 326 340 503 836 1922 2430
Size: 61 196 69 14 163 333 1086 508
Fragments arranged by size:
1086 508 333 196 163 69 61 14
AleI CACnn'nnGTG
Cuts at: 0 65 1207 2430
Size: 65 1142 1223
Fragments arranged by size:
1223 1142 65
AlwNI CAG_nnn'CTG
Cuts at: 0 258 2430
Size: 258 2172
ApoI r'AATT_y
Cuts at: 0 602 1341 2430
Size: 602 739 1089
Fragments arranged by size:
1089 739 602
BanI G'GyrC_C
Cuts at: 0 407 2123 2430
Size: 407 1716 307
Fragments arranged by size:
1716 407 307
BanII G_rGCy'C
Cuts at: 0 404 909 1391 2324 2430
Size: 404 505 482 933 106
Fragments arranged by size:
933 505 482 404 106
BbeI G_GCGC'C
Cuts at: 0 2127 2430
Size: 2127 303
BbsI GAAGACnn'nnnn_
Cuts at: 0 941 2430
Size: 941 1489
BbvCI CC'TCA_GC
Cuts at: 0 1135 2374 2430
Size: 1135 1239 56
Fragments arranged by size:
1239 1135 56
BglI GCCn_nnn'nGGC
Cuts at: 0 478 2430
Size: 478 1952
BlpI GC'TnA_GC
Cuts at: 0 262 2430
Size: 262 2168
Bme1580I G_kGCm'C
Cuts at: 0 1167 1243 2430
Size: 1167 76 1187
Fragments arranged by size:
1187 1167 76
BmgBI CAC'GTC
Cuts at: 0 106 2430
Size: 106 2324
BmrI ACTGGGnnnn_n'
Cuts at: 0 982 1464 1731 2078 2318 2430
Size: 982 482 267 347 240 112
Fragments arranged by size:
982 482 347 267 240 112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1405 2430
Size: 1405 1025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1522 2074 2430
Size: 1522 552 356
Fragments arranged by size:
1522 552 356
Bpu10I CC'TnA_GC
Cuts at: 0 403 1135 2374 2430
Size: 403 732 1239 56
Fragments arranged by size:
1239 732 403 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 310 713 960 2430
Size: 298 12 403 247 1470
Fragments arranged by size:
1470 403 298 247 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 182 274 587 2430
Size: 182 92 313 1843
Fragments arranged by size:
1843 313 182 92
BsaAI yAC'GTr
Cuts at: 0 1866 2430
Size: 1866 564
BsaHI Gr'CG_yC
Cuts at: 0 1115 1451 2124 2430
Size: 1115 336 673 306
Fragments arranged by size:
1115 673 336 306
BsaWI w'CCGG_w
Cuts at: 0 698 771 2160 2430
Size: 698 73 1389 270
Fragments arranged by size:
1389 698 270 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1146 1176 1663 1693 2430
Size: 1146 30 487 30 737
Fragments arranged by size:
1146 737 487 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 400 685 1082 1253 1687 2107 2430
Size: 379 21 285 397 171 434 420 323
Fragments arranged by size:
434 420 397 379 323 285 171 21
BseYI C'CCAG_C
Cuts at: 0 491 1193 1359 2430
Size: 491 702 166 1071
Fragments arranged by size:
1071 702 491 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1667 1885 2430
Size: 523 1144 218 545
Fragments arranged by size:
1144 545 523 218
BsiHKAI G_wGCw'C
Cuts at: 0 404 1028 1480 2430
Size: 404 624 452 950
Fragments arranged by size:
950 624 452 404
BsmI GAATG_Cn'
Cuts at: 0 1784 2430
Size: 1784 646
Bsp1286I G_dGCh'C
Cuts at: 0 404 909 1028 1167 1243 1391 1480 2324
Size: 404 505 119 139 76 148 89 844
Cuts at: 2324 2430
Size: 106
Fragments arranged by size:
844 505 404 148 139 119 106 89
76
BspEI T'CCGG_A
Cuts at: 0 2160 2430
Size: 2160 270
BspHI T'CATG_A
Cuts at: 0 1755 2430
Size: 1755 675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 129 2430
Size: 129 2301
BsrDI GCAATG_nn'
Cuts at: 0 877 2430
Size: 877 1553
BsrFI r'CCGG_y
Cuts at: 0 1904 2430
Size: 1904 526
BsrGI T'GTAC_A
Cuts at: 0 1947 2430
Size: 1947 483
BssSI C'ACGA_G
Cuts at: 0 33 2430
Size: 33 2397
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2005 2430
Size: 2005 425
Bsu36I CC'TnA_GG
Cuts at: 0 1583 2119 2430
Size: 1583 536 311
Fragments arranged by size:
1583 536 311
BtgI C'CryG_G
Cuts at: 0 1118 2034 2430
Size: 1118 916 396
Fragments arranged by size:
1118 916 396
BtsI GCAGTG_nn'
Cuts at: 0 1202 1549 1652 1684 1893 2430
Size: 1202 347 103 32 209 537
Fragments arranged by size:
1202 537 347 209 103 32
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 860 895 2430
Size: 860 35 1535
Fragments arranged by size:
1535 860 35
DraI TTT'AAA
Cuts at: 0 828 2430
Size: 828 1602
DraIII CAC_nnn'GTG
Cuts at: 0 219 1968 2430
Size: 219 1749 462
Fragments arranged by size:
1749 462 219
DrdI GACnn_nn'nnGTC
Cuts at: 0 309 2430
Size: 309 2121
EaeI y'GGCC_r
Cuts at: 0 1149 1557 1909 2236 2430
Size: 1149 408 352 327 194
Fragments arranged by size:
1149 408 352 327 194
EarI CTCTTCn'nnn_
Cuts at: 0 37 233 422 1816 2430
Size: 37 196 189 1394 614
Fragments arranged by size:
1394 614 196 189 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1036 2430
Size: 1036 1394
EcoICRI GAG'CTC
Cuts at: 0 402 2430
Size: 402 2028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 257 326 340 503 836 1522 1922
Size: 61 196 69 14 163 333 686 400
Cuts at: 1922 2074 2430
Size: 152 356
Fragments arranged by size:
686 400 356 333 196 163 152 69
61 14
EcoO109I rG'GnC_Cy
Cuts at: 0 47 733 810 1252 2370 2430
Size: 47 686 77 442 1118 60
Fragments arranged by size:
1118 686 442 77 60 47
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 262 2430
Size: 262 2168
HaeII r_GCGC'y
Cuts at: 0 2127 2430
Size: 2127 303
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 549 581 711 743 1128 1160
Size: 109 32 408 32 130 32 385 32
Cuts at: 1160 1210 1242 1663 1695 2430
Size: 50 32 421 32 735
Fragments arranged by size:
735 421 408 385 130 109 50 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1770 1878 2430
Size: 1770 108 552
Fragments arranged by size:
1770 552 108
HindIII A'AGCT_T
Cuts at: 0 1051 1633 2430
Size: 1051 582 797
Fragments arranged by size:
1051 797 582
KasI G'GCGC_C
Cuts at: 0 2123 2430
Size: 2123 307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 643 1982 2430
Size: 413 230 1339 448
Fragments arranged by size:
1339 448 413 230
MscI TGG'CCA
Cuts at: 0 1559 2430
Size: 1559 871
MslI CAynn'nnrTG
Cuts at: 0 65 1207 1702 1864 2430
Size: 65 1142 495 162 566
Fragments arranged by size:
1142 566 495 162 65
MspA1I CmG'CkG
Cuts at: 0 495 1915 2430
Size: 495 1420 515
Fragments arranged by size:
1420 515 495
NarI GG'CG_CC
Cuts at: 0 2124 2430
Size: 2124 306
NcoI C'CATG_G
Cuts at: 0 2034 2430
Size: 2034 396
NspI r_CATG'y
Cuts at: 0 1876 2430
Size: 1876 554
PasI CC'CwG_GG
Cuts at: 0 171 2430
Size: 171 2259
PflMI CCAn_nnn'nTGG
Cuts at: 0 1544 2430
Size: 1544 886
PfoI T'CCnGG_A
Cuts at: 0 553 2051 2430
Size: 553 1498 379
Fragments arranged by size:
1498 553 379
PmlI CAC'GTG
Cuts at: 0 1866 2430
Size: 1866 564
PpuMI rG'GwC_Cy
Cuts at: 0 47 810 1252 2430
Size: 47 763 442 1178
Fragments arranged by size:
1178 763 442 47
PvuII CAG'CTG
Cuts at: 0 495 1915 2430
Size: 495 1420 515
Fragments arranged by size:
1420 515 495
SacI G_AGCT'C
Cuts at: 0 404 2430
Size: 404 2026
SexAI A'CCwGG_T
Cuts at: 0 215 2411 2430
Size: 215 2196 19
Fragments arranged by size:
2196 215 19
SfcI C'TryA_G
Cuts at: 0 860 2355 2430
Size: 860 1495 75
Fragments arranged by size:
1495 860 75
SfoI GGC'GCC
Cuts at: 0 2125 2430
Size: 2125 305
SgrAI Cr'CCGG_yG
Cuts at: 0 1904 2430
Size: 1904 526
SmlI C'TyrA_G
Cuts at: 0 277 325 728 939 2430
Size: 277 48 403 211 1491
Fragments arranged by size:
1491 403 277 211 48
StuI AGG'CCT
Cuts at: 0 2042 2430
Size: 2042 388
StyI C'CwwG_G
Cuts at: 0 1001 1433 2034 2239 2430
Size: 1001 432 601 205 191
Fragments arranged by size:
1001 601 432 205 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1631 1692 2025 2430
Size: 1631 61 333 405
Fragments arranged by size:
1631 405 333 61
TatI w'GTAC_w
Cuts at: 0 1739 1947 2430
Size: 1739 208 483
Fragments arranged by size:
1739 483 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 203 235 1146 1178 2430
Size: 203 32 911 32 1252
Fragments arranged by size:
1252 911 203 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2332 2430
Size: 2332 98
XbaI T'CTAG_A
Cuts at: 0 108 930 1762 2430
Size: 108 822 832 668
Fragments arranged by size:
832 822 668 108
XmnI GAAnn'nnTTC
Cuts at: 0 182 2430
Size: 182 2248
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApoI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI Bsu36I
BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NarI NcoI
NspI PasI PflMI PfoI PmlI PpuMI PvuII SacI
SexAI SfcI SfoI SgrAI SmlI StuI StyI TaqII
TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BmtI BsaBI BsiEI BsiWI BsmBI BsrBI BssHII BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI EagI EcoNI
EcoRI EcoRV FseI FspI FspAI HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SgfI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XcmI XhoI XmaI ZraI