Restriction map for
pF1KSDB1384
(Linear) (Six-base) MAPSORT of: KIBB1384.seq Check: 1188 from: 1 to: 2022
KIBB1384.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1408 2022
Size: 1408 614
AccI GT'mk_AC
Cuts at: 0 1805 2022
Size: 1805 217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1253 2022
Size: 1253 769
AleI CACnn'nnGTG
Cuts at: 0 10 647 2022
Size: 10 637 1375
Fragments arranged by size:
1375 637 10
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1991 2022
Size: 1991 31
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 646 678 2022
Size: 646 32 1344
Fragments arranged by size:
1344 646 32
AlwNI CAG_nnn'CTG
Cuts at: 0 171 1002 1137 1465 2022
Size: 171 831 135 328 557
Fragments arranged by size:
831 557 328 171 135
ApoI r'AATT_y
Cuts at: 0 675 1212 1382 1483 1590 2022
Size: 675 537 170 101 107 432
Fragments arranged by size:
675 537 432 170 107 101
BanII G_rGCy'C
Cuts at: 0 1336 2022
Size: 1336 686
BbsI GAAGACnn'nnnn_
Cuts at: 0 1041 1172 2022
Size: 1041 131 850
Fragments arranged by size:
1041 850 131
BbvCI CC'TCA_GC
Cuts at: 0 335 1054 1424 1520 2022
Size: 335 719 370 96 502
Fragments arranged by size:
719 502 370 335 96
BciVI GTATCCnnnnn_n'
Cuts at: 0 857 2022
Size: 857 1165
BclI T'GATC_A
Cuts at: 0 1467 2022
Size: 1467 555
BfrBI ATG'CAT
Cuts at: 0 1680 2022
Size: 1680 342
BglI GCCn_nnn'nGGC
Cuts at: 0 889 2022
Size: 889 1133
BglII A'GATC_T
Cuts at: 0 619 2022
Size: 619 1403
BmrI ACTGGGnnnn_n'
Cuts at: 0 1186 1538 2022
Size: 1186 352 484
Fragments arranged by size:
1186 484 352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 285 536 1202 2022
Size: 87 198 251 666 820
Fragments arranged by size:
820 666 251 198 87
Bpu10I CC'TnA_GC
Cuts at: 0 335 1054 1424 1520 2022
Size: 335 719 370 96 502
Fragments arranged by size:
719 502 370 335 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 423 2022
Size: 99 324 1599
Fragments arranged by size:
1599 324 99
BsiEI CG_ry'CG
Cuts at: 0 11 2022
Size: 11 2011
BsmI GAATG_Cn'
Cuts at: 0 1897 1965 2022
Size: 1897 68 57
Fragments arranged by size:
1897 68 57
Bsp1286I G_dGCh'C
Cuts at: 0 1336 2022
Size: 1336 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 487 747 1408 2022
Size: 23 464 260 661 614
Fragments arranged by size:
661 614 464 260 23
BsrDI GCAATG_nn'
Cuts at: 0 374 2022
Size: 374 1648
BsrFI r'CCGG_y
Cuts at: 0 17 28 2022
Size: 17 11 1994
Fragments arranged by size:
1994 17 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 345 2022
Size: 345 1677
BstEII G'GTnAC_C
Cuts at: 0 1409 2022
Size: 1409 613
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1066 1451 2022
Size: 1066 385 571
Fragments arranged by size:
1066 571 385
BstYI r'GATC_y
Cuts at: 0 619 2022
Size: 619 1403
BtgI C'CryG_G
Cuts at: 0 5 2022
Size: 5 2017
BtsI GCAGTG_nn'
Cuts at: 0 404 728 1789 2022
Size: 404 324 1061 233
Fragments arranged by size:
1061 404 324 233
DraI TTT'AAA
Cuts at: 0 1118 2022
Size: 1118 904
EaeI y'GGCC_r
Cuts at: 0 2 8 2022
Size: 2 6 2014
Fragments arranged by size:
2014 6 2
EagI C'GGCC_G
Cuts at: 0 8 2022
Size: 8 2014
EarI CTCTTCn'nnn_
Cuts at: 0 874 2022
Size: 874 1148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 285 536 1202 1253 2022
Size: 87 198 251 666 51 769
Fragments arranged by size:
769 666 251 198 87 51
EcoO109I rG'GnC_Cy
Cuts at: 0 999 1343 2022
Size: 999 344 679
Fragments arranged by size:
999 679 344
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1611 2022
Size: 1611 411
FspI TGC'GCA
Cuts at: 0 473 2022
Size: 473 1549
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 315 347 611 643 1131 1163 2022
Size: 315 32 264 32 488 32 859
Fragments arranged by size:
859 488 315 264 32 32 32
HincII GTy'rAC
Cuts at: 0 558 1787 1806 2022
Size: 558 1229 19 216
Fragments arranged by size:
1229 558 216 19
HindIII A'AGCT_T
Cuts at: 0 716 1819 2022
Size: 716 1103 203
Fragments arranged by size:
1103 716 203
HpaI GTT'AAC
Cuts at: 0 558 2022
Size: 558 1464
MfeI C'AATT_G
Cuts at: 0 1080 2022
Size: 1080 942
MscI TGG'CCA
Cuts at: 0 4 2022
Size: 4 2018
MslI CAynn'nnrTG
Cuts at: 0 10 647 733 749 1958 2022
Size: 10 637 86 16 1209 64
Fragments arranged by size:
1209 637 86 64 16 10
NaeI GCC'GGC
Cuts at: 0 19 30 2022
Size: 19 11 1992
Fragments arranged by size:
1992 19 11
NgoMIV G'CCGG_C
Cuts at: 0 17 28 2022
Size: 17 11 1994
Fragments arranged by size:
1994 17 11
NsiI A_TGCA'T
Cuts at: 0 1682 2022
Size: 1682 340
PasI CC'CwG_GG
Cuts at: 0 408 2022
Size: 408 1614
PflMI CCAn_nnn'nTGG
Cuts at: 0 719 2022
Size: 719 1303
PpuMI rG'GwC_Cy
Cuts at: 0 999 1343 2022
Size: 999 344 679
Fragments arranged by size:
999 679 344
PsiI TTA'TAA
Cuts at: 0 130 2022
Size: 130 1892
PstI C_TGCA'G
Cuts at: 0 399 1980 2022
Size: 399 1581 42
Fragments arranged by size:
1581 399 42
SalI G'TCGA_C
Cuts at: 0 1804 2022
Size: 1804 218
SanDI GG'GwC_CC
Cuts at: 0 1343 2022
Size: 1343 679
ScaI AGT'ACT
Cuts at: 0 1813 2022
Size: 1813 209
SexAI A'CCwGG_T
Cuts at: 0 1062 2022
Size: 1062 960
SfcI C'TryA_G
Cuts at: 0 395 531 537 1976 2022
Size: 395 136 6 1439 46
Fragments arranged by size:
1439 395 136 46 6
SspI AAT'ATT
Cuts at: 0 1021 2022
Size: 1021 1001
StuI AGG'CCT
Cuts at: 0 1645 2022
Size: 1645 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1652 2022
Size: 1652 370
TatI w'GTAC_w
Cuts at: 0 416 1811 2022
Size: 416 1395 211
Fragments arranged by size:
1395 416 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 1850 1882 2022
Size: 34 32 1784 32 140
Fragments arranged by size:
1784 140 34 32 32
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AloI AlwNI ApoI
BanII BbsI BbvCI BciVI BclI BfrBI BglI BglII
BmrI BpmI Bpu10I BsgI BsiEI BsmI Bsp1286I BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI
DraI EaeI EagI EarI Eco57MI EcoO109I FalI FspI
Hin4I HincII HindIII HpaI MfeI MscI MslI NaeI
NgoMIV NsiI PasI PflMI PpuMI PsiI PstI SalI
SanDI ScaI SexAI SfcI SspI StuI TaqII TatI
TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BcgI BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspAI HaeII KasI KpnI MluI MmeI MspA1I
NarI NcoI NdeI NheI NotI NruI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI