Restriction map for
pF1KB1325
(Linear) (Six-base) MAPSORT of: KIBB1325.seq Check: 1067 from: 1 to: 2262
KIBB1325.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 78 615 2262
Size: 78 537 1647
Fragments arranged by size:
1647 537 78
AccI GT'mk_AC
Cuts at: 0 7 491 2262
Size: 7 484 1771
Fragments arranged by size:
1771 484 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 381 1417 2262
Size: 312 69 1036 845
Fragments arranged by size:
1036 845 312 69
AfeI AGC'GCT
Cuts at: 0 1177 2262
Size: 1177 1085
AlwNI CAG_nnn'CTG
Cuts at: 0 370 2262
Size: 370 1892
ApaI G_GGCC'C
Cuts at: 0 1297 2262
Size: 1297 965
AvaI C'yCGr_G
Cuts at: 0 568 741 2262
Size: 568 173 1521
Fragments arranged by size:
1521 568 173
BamHI G'GATC_C
Cuts at: 0 1485 1694 2262
Size: 1485 209 568
Fragments arranged by size:
1485 568 209
BanI G'GyrC_C
Cuts at: 0 978 1704 2262
Size: 978 726 558
Fragments arranged by size:
978 726 558
BanII G_rGCy'C
Cuts at: 0 292 533 938 1297 2119 2262
Size: 292 241 405 359 822 143
Fragments arranged by size:
822 405 359 292 241 143
BbsI GAAGACnn'nnnn_
Cuts at: 0 1228 1234 2262
Size: 1228 6 1028
Fragments arranged by size:
1228 1028 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1740 1774 2262
Size: 1740 34 488
Fragments arranged by size:
1740 488 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1459 2262
Size: 1459 803
BclI T'GATC_A
Cuts at: 0 1794 2262
Size: 1794 468
BglI GCCn_nnn'nGGC
Cuts at: 0 202 1990 2222 2262
Size: 202 1788 232 40
Fragments arranged by size:
1788 232 202 40
BglII A'GATC_T
Cuts at: 0 869 1082 2262
Size: 869 213 1180
Fragments arranged by size:
1180 869 213
BlpI GC'TnA_GC
Cuts at: 0 2024 2262
Size: 2024 238
Bme1580I G_kGCm'C
Cuts at: 0 1297 2262
Size: 1297 965
BmrI ACTGGGnnnn_n'
Cuts at: 0 220 358 2013 2262
Size: 220 138 1655 249
Fragments arranged by size:
1655 249 220 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1101 2262
Size: 1101 1161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 2262
Size: 1378 884
Bpu10I CC'TnA_GC
Cuts at: 0 250 532 1387 1597 2262
Size: 250 282 855 210 665
Fragments arranged by size:
855 665 282 250 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 1096 1960 2262
Size: 306 790 864 302
Fragments arranged by size:
864 790 306 302
BsaI GGTCTCn'nnnn_
Cuts at: 0 508 1978 2262
Size: 508 1470 284
Fragments arranged by size:
1470 508 284
BsaHI Gr'CG_yC
Cuts at: 0 1187 2262
Size: 1187 1075
BsaWI w'CCGG_w
Cuts at: 0 787 2262
Size: 787 1475
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 70 1705 1735 2262
Size: 40 30 1635 30 527
Fragments arranged by size:
1635 527 40 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 430 541 856 946 1713 2262
Size: 49 381 111 315 90 767 549
Fragments arranged by size:
767 549 381 315 111 90 49
BseYI C'CCAG_C
Cuts at: 0 125 386 813 825 2262
Size: 125 261 427 12 1437
Fragments arranged by size:
1437 427 261 125 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 2262
Size: 116 2146
BsiEI CG_ry'CG
Cuts at: 0 1691 2262
Size: 1691 571
BsiHKAI G_wGCw'C
Cuts at: 0 292 533 938 1393 2030 2098 2119 2262
Size: 292 241 405 455 637 68 21 143
Fragments arranged by size:
637 455 405 292 241 143 68 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 877 2262
Size: 877 1385
Bsp1286I G_dGCh'C
Cuts at: 0 292 533 938 1297 1393 2030 2098 2119
Size: 292 241 405 359 96 637 68 21
Cuts at: 2119 2262
Size: 143
Fragments arranged by size:
637 405 359 292 241 143 96 68
21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 615 2262
Size: 78 537 1647
Fragments arranged by size:
1647 537 78
BsrFI r'CCGG_y
Cuts at: 0 1923 2262
Size: 1923 339
BstEII G'GTnAC_C
Cuts at: 0 630 2262
Size: 630 1632
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1497 2262
Size: 1497 765
BstYI r'GATC_y
Cuts at: 0 232 869 1082 1485 1694 2262
Size: 232 637 213 403 209 568
Fragments arranged by size:
637 568 403 232 213 209
BstZ17I GTA'TAC
Cuts at: 0 492 2262
Size: 492 1770
BtgI C'CryG_G
Cuts at: 0 293 452 1025 1640 1834 1853 2262
Size: 293 159 573 615 194 19 409
Fragments arranged by size:
615 573 409 293 194 159 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1941 2262
Size: 1941 321
BtsI GCAGTG_nn'
Cuts at: 0 1520 1583 2262
Size: 1520 63 679
Fragments arranged by size:
1520 679 63
DraIII CAC_nnn'GTG
Cuts at: 0 951 2262
Size: 951 1311
EaeI y'GGCC_r
Cuts at: 0 114 375 455 893 1688 1831 2262
Size: 114 261 80 438 795 143 431
Fragments arranged by size:
795 438 431 261 143 114 80
EagI C'GGCC_G
Cuts at: 0 1688 2262
Size: 1688 574
EarI CTCTTCn'nnn_
Cuts at: 0 2071 2262
Size: 2071 191
EcoICRI GAG'CTC
Cuts at: 0 290 531 936 2117 2262
Size: 290 241 405 1181 145
Fragments arranged by size:
1181 405 290 241 145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 381 1378 1417 2262
Size: 312 69 997 39 845
Fragments arranged by size:
997 845 312 69 39
EcoO109I rG'GnC_Cy
Cuts at: 0 39 392 589 1294 2262
Size: 39 353 197 705 968
Fragments arranged by size:
968 705 353 197 39
EcoRV GAT'ATC
Cuts at: 0 793 2262
Size: 793 1469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 254 624 2262
Size: 254 370 1638
Fragments arranged by size:
1638 370 254
HaeII r_GCGC'y
Cuts at: 0 924 1179 2262
Size: 924 255 1083
Fragments arranged by size:
1083 924 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 364 396 777 809 1074 1103
Size: 40 32 292 32 381 32 265 29
Cuts at: 1103 1106 1135 2262
Size: 3 29 1127
Fragments arranged by size:
1127 381 292 265 40 32 32 32
29 29 3
HincII GTy'rAC
Cuts at: 0 8 2262
Size: 8 2254
HindIII A'AGCT_T
Cuts at: 0 257 2262
Size: 257 2005
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 2262
Size: 719 1543
MscI TGG'CCA
Cuts at: 0 116 377 895 2262
Size: 116 261 518 1367
Fragments arranged by size:
1367 518 261 116
MspA1I CmG'CkG
Cuts at: 0 813 2213 2262
Size: 813 1400 49
Fragments arranged by size:
1400 813 49
NcoI C'CATG_G
Cuts at: 0 293 1640 1834 2262
Size: 293 1347 194 428
Fragments arranged by size:
1347 428 293 194
PasI CC'CwG_GG
Cuts at: 0 593 1944 2043 2262
Size: 593 1351 99 219
Fragments arranged by size:
1351 593 219 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 992 2262
Size: 992 1270
PfoI T'CCnGG_A
Cuts at: 0 611 1355 2262
Size: 611 744 907
Fragments arranged by size:
907 744 611
PpuMI rG'GwC_Cy
Cuts at: 0 39 392 589 2262
Size: 39 353 197 1673
Fragments arranged by size:
1673 353 197 39
PspOMI G'GGCC_C
Cuts at: 0 1293 2262
Size: 1293 969
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1639 1671 2262
Size: 1639 32 591
Fragments arranged by size:
1639 591 32
PstI C_TGCA'G
Cuts at: 0 89 626 971 1527 1703 2262
Size: 89 537 345 556 176 559
Fragments arranged by size:
559 556 537 345 176 89
PvuII CAG'CTG
Cuts at: 0 813 2262
Size: 813 1449
SacI G_AGCT'C
Cuts at: 0 292 533 938 2119 2262
Size: 292 241 405 1181 143
Fragments arranged by size:
1181 405 292 241 143
SalI G'TCGA_C
Cuts at: 0 6 2262
Size: 6 2256
SapI GCTCTTCn'nnn_
Cuts at: 0 2071 2262
Size: 2071 191
SbfI CC_TGCA'GG
Cuts at: 0 89 626 1703 2262
Size: 89 537 1077 559
Fragments arranged by size:
1077 559 537 89
SexAI A'CCwGG_T
Cuts at: 0 41 1142 2262
Size: 41 1101 1120
Fragments arranged by size:
1120 1101 41
SfcI C'TryA_G
Cuts at: 0 85 622 967 1523 1699 1905 2262
Size: 85 537 345 556 176 206 357
Fragments arranged by size:
556 537 357 345 206 176 85
SmlI C'TyrA_G
Cuts at: 0 285 741 1075 1975 2262
Size: 285 456 334 900 287
Fragments arranged by size:
900 456 334 287 285
StuI AGG'CCT
Cuts at: 0 1735 1861 2129 2262
Size: 1735 126 268 133
Fragments arranged by size:
1735 268 133 126
StyI C'CwwG_G
Cuts at: 0 293 599 974 1430 1490 1640 1730 1834
Size: 293 306 375 456 60 150 90 104
Cuts at: 1834 2189 2262
Size: 355 73
Fragments arranged by size:
456 375 355 306 293 150 104 90
73 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1743 1891 1995 2244 2262
Size: 1743 148 104 249 18
Fragments arranged by size:
1743 249 148 104 18
TatI w'GTAC_w
Cuts at: 0 1895 2262
Size: 1895 367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 200 232 1512 1544 2262
Size: 200 32 1280 32 718
Fragments arranged by size:
1280 718 200 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 546 1479 2262
Size: 546 933 783
Fragments arranged by size:
933 783 546
XhoI C'TCGA_G
Cuts at: 0 741 2262
Size: 741 1521
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MscI MspA1I NcoI PasI PflMI PfoI PpuMI
PspOMI PsrI PstI PvuII SacI SalI SapI SbfI
SexAI SfcI SmlI StuI StyI TaqII TatI TstI
XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BfrBI BmgBI BmtI BsaAI BsaBI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI Bsu36I ClaI CspCI DraI DrdI
EciI EcoNI EcoRI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII
SanDI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI
XmnI ZraI