(Linear) (Six-base) MAPSORT of: KIBB0998.seq  Check: 9680  from: 1  to: 690



KIBB0998.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     349     690 
   Size:        349     341
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      81     113     690 
   Size:         81      32     577

  Fragments arranged by size: 

                 577      81      32
 
ApoI r'AATT_y

Cuts at:      0     469     690 
   Size:        469     221
 
AvaI C'yCGr_G

Cuts at:      0     332     690 
   Size:        332     358
 
AvrII C'CTAG_G

Cuts at:      0      36     690 
   Size:         36     654
 
BamHI G'GATC_C

Cuts at:      0     425     690 
   Size:        425     265
 
BanI G'GyrC_C

Cuts at:      0     109     453     690 
   Size:        109     344     237

  Fragments arranged by size: 

                 344     237     109
 
BanII G_rGCy'C

Cuts at:      0     622     690 
   Size:        622      68
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     309     690 
   Size:        309     381
 
Bme1580I G_kGCm'C

Cuts at:      0      61     690 
   Size:         61     629
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     180     690 
   Size:        180     510
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     261     690 
   Size:        261     429
 
BseYI C'CCAG_C

Cuts at:      0      82     690 
   Size:         82     608
 
BsiHKAI G_wGCw'C

Cuts at:      0     622     690 
   Size:        622      68
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     622     690 
   Size:         61     561      68

  Fragments arranged by size: 

                 561      68      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     349     690 
   Size:        349     341
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     308     690 
   Size:        308     382
 
BstYI r'GATC_y

Cuts at:      0     425     690 
   Size:        425     265
 
DraIII CAC_nnn'GTG

Cuts at:      0     188     690 
   Size:        188     502
 
EcoICRI GAG'CTC

Cuts at:      0     620     690 
   Size:        620      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0     144     690 
   Size:        144     546
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     299     331     427     459     690 
   Size:        299      32      96      32     231

  Fragments arranged by size: 

                 299     231      96      32      32
 
HincII GTy'rAC

Cuts at:      0     637     690 
   Size:        637      53
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     599     690 
   Size:         42     557      91

  Fragments arranged by size: 

                 557      91      42
 
MslI CAynn'nnrTG

Cuts at:      0     250     690 
   Size:        250     440
 
MspA1I CmG'CkG

Cuts at:      0      86     223     690 
   Size:         86     137     467

  Fragments arranged by size: 

                 467     137      86
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      24     631     690 
   Size:         24     607      59

  Fragments arranged by size: 

                 607      59      24
 
PvuII CAG'CTG

Cuts at:      0      86     690 
   Size:         86     604
 
SacI G_AGCT'C

Cuts at:      0     622     690 
   Size:        622      68
 
SfcI C'TryA_G

Cuts at:      0     198     669     690 
   Size:        198     471      21

  Fragments arranged by size: 

                 471     198      21
 
StuI AGG'CCT

Cuts at:      0      41     386     630     690 
   Size:         41     345     244      60

  Fragments arranged by size: 

                 345     244      60      41
 
StyI C'CwwG_G

Cuts at:      0      36     140     378     625     690 
   Size:         36     104     238     247      65

  Fragments arranged by size: 

                 247     238     104      65      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     199     690 
   Size:        199     491
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     144     176     690 
   Size:        144      32     514

  Fragments arranged by size: 

                 514     144      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     345     690 
   Size:        345     345
 

 Enzymes that do cut:

     AarI     AloI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbsI Bme1580I     BmrI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI
   BstAPI    BstYI   DraIII  EcoICRI EcoO109I    Hin4I   HincII     MmeI
     MslI   MspA1I    PflMI    PvuII     SacI     SfcI     StuI     StyI
    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI