Restriction map for
pF1KB0983
(Linear) (Six-base) MAPSORT of: KIBB0983.seq Check: 5529 from: 1 to: 720
KIBB0983.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
Acc65I G'GTAC_C
Cuts at: 0 282 720
Size: 282 438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 43 720
Size: 43 677
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 720
Size: 217 32 471
Fragments arranged by size:
471 217 32
AlwNI CAG_nnn'CTG
Cuts at: 0 388 457 720
Size: 388 69 263
Fragments arranged by size:
388 263 69
ApaI G_GGCC'C
Cuts at: 0 601 720
Size: 601 119
ApoI r'AATT_y
Cuts at: 0 114 720
Size: 114 606
AvaI C'yCGr_G
Cuts at: 0 278 623 720
Size: 278 345 97
Fragments arranged by size:
345 278 97
BanI G'GyrC_C
Cuts at: 0 85 282 720
Size: 85 197 438
Fragments arranged by size:
438 197 85
BanII G_rGCy'C
Cuts at: 0 492 601 720
Size: 492 109 119
Fragments arranged by size:
492 119 109
BciVI GTATCCnnnnn_n'
Cuts at: 0 67 243 720
Size: 67 176 477
Fragments arranged by size:
477 176 67
Bme1580I G_kGCm'C
Cuts at: 0 520 601 720
Size: 520 81 119
Fragments arranged by size:
520 119 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 683 720
Size: 683 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 323 506 539 720
Size: 314 9 183 33 181
Fragments arranged by size:
314 183 181 33 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 579 654 720
Size: 579 75 66
Fragments arranged by size:
579 75 66
BsaHI Gr'CG_yC
Cuts at: 0 631 720
Size: 631 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 537 567 720
Size: 537 30 153
Fragments arranged by size:
537 153 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 350 410 560 720
Size: 350 60 150 160
Fragments arranged by size:
350 160 150 60
BseYI C'CCAG_C
Cuts at: 0 413 720
Size: 413 307
BsmI GAATG_Cn'
Cuts at: 0 168 707 720
Size: 168 539 13
Fragments arranged by size:
539 168 13
Bsp1286I G_dGCh'C
Cuts at: 0 492 520 601 720
Size: 492 28 81 119
Fragments arranged by size:
492 119 81 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 408 720
Size: 408 312
BsrDI GCAATG_nn'
Cuts at: 0 272 720
Size: 272 448
BstAPI GCAn_nnn'nTGC
Cuts at: 0 457 720
Size: 457 263
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 720
Size: 243 477
BtgI C'CryG_G
Cuts at: 0 593 720
Size: 593 127
EaeI y'GGCC_r
Cuts at: 0 69 720
Size: 69 651
EarI CTCTTCn'nnn_
Cuts at: 0 432 720
Size: 432 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 512 720
Size: 512 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 323 506 539 720
Size: 314 9 183 33 181
Fragments arranged by size:
314 183 181 33 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 392 623 671 695 720
Size: 392 231 48 24 25
Fragments arranged by size:
392 231 48 25 24
EcoO109I rG'GnC_Cy
Cuts at: 0 307 366 385 720
Size: 307 59 19 335
Fragments arranged by size:
335 307 59 19
KpnI G_GTAC'C
Cuts at: 0 286 720
Size: 286 434
MfeI C'AATT_G
Cuts at: 0 605 720
Size: 605 115
MslI CAynn'nnrTG
Cuts at: 0 241 720
Size: 241 479
MspA1I CmG'CkG
Cuts at: 0 328 346 454 720
Size: 328 18 108 266
Fragments arranged by size:
328 266 108 18
NcoI C'CATG_G
Cuts at: 0 593 720
Size: 593 127
NdeI CA'TA_TG
Cuts at: 0 110 720
Size: 110 610
NspI r_CATG'y
Cuts at: 0 645 720
Size: 645 75
PasI CC'CwG_GG
Cuts at: 0 310 720
Size: 310 410
PflMI CCAn_nnn'nTGG
Cuts at: 0 292 607 720
Size: 292 315 113
Fragments arranged by size:
315 292 113
PfoI T'CCnGG_A
Cuts at: 0 651 720
Size: 651 69
PspOMI G'GGCC_C
Cuts at: 0 597 720
Size: 597 123
PspXI vC'TCGA_Gb
Cuts at: 0 623 720
Size: 623 97
PstI C_TGCA'G
Cuts at: 0 32 720
Size: 32 688
PvuII CAG'CTG
Cuts at: 0 328 346 454 720
Size: 328 18 108 266
Fragments arranged by size:
328 266 108 18
SexAI A'CCwGG_T
Cuts at: 0 576 720
Size: 576 144
SfcI C'TryA_G
Cuts at: 0 28 42 446 720
Size: 28 14 404 274
Fragments arranged by size:
404 274 28 14
SmlI C'TyrA_G
Cuts at: 0 623 720
Size: 623 97
SphI G_CATG'C
Cuts at: 0 645 720
Size: 645 75
StyI C'CwwG_G
Cuts at: 0 593 720
Size: 593 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 606 720
Size: 606 114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 370 720
Size: 370 350
XhoI C'TCGA_G
Cuts at: 0 623 720
Size: 623 97
Enzymes that do cut:
Acc65I AlfI AloI AlwNI ApaI ApoI AvaI BanI
BanII BciVI Bme1580I BplI BpmI BsaI BsaHI BsaXI
BseRI BseYI BsmI Bsp1286I BspMI BsrDI BstAPI BstXI
BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I KpnI
MfeI MslI MspA1I NcoI NdeI NspI PasI PflMI
PfoI PspOMI PspXI PstI PvuII SexAI SfcI SmlI
SphI StyI TaqII XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI MluI MmeI MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XmaI XmnI ZraI