Restriction map for
pF1KB0966
(Linear) (Six-base) MAPSORT of: KIBB0966.seq Check: 6658 from: 1 to: 1281
KIBB0966.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 705 873 1281
Size: 621 84 168 408
Fragments arranged by size:
621 408 168 84
AflII C'TTAA_G
Cuts at: 0 315 1230 1281
Size: 315 915 51
Fragments arranged by size:
915 315 51
AflIII A'CryG_T
Cuts at: 0 105 235 397 1281
Size: 105 130 162 884
Fragments arranged by size:
884 162 130 105
AleI CACnn'nnGTG
Cuts at: 0 236 1100 1281
Size: 236 864 181
Fragments arranged by size:
864 236 181
AlwNI CAG_nnn'CTG
Cuts at: 0 37 1281
Size: 37 1244
ApoI r'AATT_y
Cuts at: 0 587 1007 1051 1281
Size: 587 420 44 230
Fragments arranged by size:
587 420 230 44
BanI G'GyrC_C
Cuts at: 0 1250 1281
Size: 1250 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 478 879 1281
Size: 478 401 402
Fragments arranged by size:
478 402 401
BfrBI ATG'CAT
Cuts at: 0 502 522 1281
Size: 502 20 759
Fragments arranged by size:
759 502 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 785 953 1205 1281
Size: 701 84 168 252 76
Fragments arranged by size:
701 252 168 84 76
BsaAI yAC'GTr
Cuts at: 0 398 1281
Size: 398 883
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 1281
Size: 421 860
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 552 636 888 1140 1224 1281
Size: 339 213 84 252 252 84 57
Fragments arranged by size:
339 252 252 213 84 84 57
BsiHKAI G_wGCw'C
Cuts at: 0 487 1281
Size: 487 794
BsmI GAATG_Cn'
Cuts at: 0 703 1048 1281
Size: 703 345 233
Fragments arranged by size:
703 345 233
Bsp1286I G_dGCh'C
Cuts at: 0 487 1281
Size: 487 794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 115 1281
Size: 115 1166
BsrDI GCAATG_nn'
Cuts at: 0 792 1281
Size: 792 489
BsrGI T'GTAC_A
Cuts at: 0 828 1281
Size: 828 453
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1043 1281
Size: 1043 238
BtgI C'CryG_G
Cuts at: 0 216 1281
Size: 216 1065
BtsI GCAGTG_nn'
Cuts at: 0 104 540 876 960 1212 1265 1281
Size: 104 436 336 84 252 53 16
Fragments arranged by size:
436 336 252 104 84 53 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 774 809 1281
Size: 774 35 472
Fragments arranged by size:
774 472 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 571 1281
Size: 571 710
EaeI y'GGCC_r
Cuts at: 0 975 1281
Size: 975 306
EarI CTCTTCn'nnn_
Cuts at: 0 59 1281
Size: 59 1222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 701 705 785 873 953 1205 1281
Size: 621 80 4 80 88 80 252 76
Fragments arranged by size:
621 252 88 80 80 80 76 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 318 1281
Size: 318 963
EcoO109I rG'GnC_Cy
Cuts at: 0 34 1281
Size: 34 1247
EcoRI G'AATT_C
Cuts at: 0 587 1007 1281
Size: 587 420 274
Fragments arranged by size:
587 420 274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 586 670 754 1281
Size: 586 84 84 527
Fragments arranged by size:
586 527 84 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 1281
Size: 52 32 1197
Fragments arranged by size:
1197 52 32
HindIII A'AGCT_T
Cuts at: 0 548 1281
Size: 548 733
MfeI C'AATT_G
Cuts at: 0 341 1281
Size: 341 940
MscI TGG'CCA
Cuts at: 0 977 1281
Size: 977 304
MslI CAynn'nnrTG
Cuts at: 0 194 236 1100 1200 1281
Size: 194 42 864 100 81
Fragments arranged by size:
864 194 100 81 42
NcoI C'CATG_G
Cuts at: 0 216 1281
Size: 216 1065
NdeI CA'TA_TG
Cuts at: 0 160 1197 1281
Size: 160 1037 84
Fragments arranged by size:
1037 160 84
NsiI A_TGCA'T
Cuts at: 0 504 524 1281
Size: 504 20 757
Fragments arranged by size:
757 504 20
NspI r_CATG'y
Cuts at: 0 109 239 248 1281
Size: 109 130 9 1033
Fragments arranged by size:
1033 130 109 9
PciI A'CATG_T
Cuts at: 0 105 235 1281
Size: 105 130 1046
Fragments arranged by size:
1046 130 105
PshAI GACnn'nnGTC
Cuts at: 0 7 1281
Size: 7 1274
PsiI TTA'TAA
Cuts at: 0 779 863 1281
Size: 779 84 418
Fragments arranged by size:
779 418 84
PstI C_TGCA'G
Cuts at: 0 1272 1281
Size: 1272 9
SfcI C'TryA_G
Cuts at: 0 92 1224 1268 1281
Size: 92 1132 44 13
Fragments arranged by size:
1132 92 44 13
SmlI C'TyrA_G
Cuts at: 0 315 1230 1281
Size: 315 915 51
Fragments arranged by size:
915 315 51
SspI AAT'ATT
Cuts at: 0 802 1281
Size: 802 479
StuI AGG'CCT
Cuts at: 0 337 690 858 1110 1222 1281
Size: 337 353 168 252 112 59
Fragments arranged by size:
353 337 252 168 112 59
StyI C'CwwG_G
Cuts at: 0 119 216 1281
Size: 119 97 1065
Fragments arranged by size:
1065 119 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 424 1281
Size: 424 857
TatI w'GTAC_w
Cuts at: 0 828 1281
Size: 828 453
XmnI GAAnn'nnTTC
Cuts at: 0 418 550 634 886 1281
Size: 418 132 84 252 395
Fragments arranged by size:
418 395 252 132 84
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApoI BanI BbsI
BfrBI BpmI BsaAI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrGI BstAPI BtgI BtsI CspCI DrdI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HindIII MfeI MscI MslI NcoI NdeI NsiI NspI
PciI PshAI PsiI PstI SfcI SmlI SspI StuI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI DraI DraIII EagI EciI EcoICRI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI MmeI MspA1I NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI