(Linear) (Six-base) MAPSORT of: KIBB0934.seq  Check: 1806  from: 1  to: 1143



KIBB0934.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:25  ..

AccI GT'mk_AC

Cuts at:      0      44     800    1143 
   Size:         44     756     343

  Fragments arranged by size: 

                 756     343      44
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     998    1143 
   Size:        203     795     145

  Fragments arranged by size: 

                 795     203     145
 
AflII C'TTAA_G

Cuts at:      0     667    1143 
   Size:        667     476
 
AlwNI CAG_nnn'CTG

Cuts at:      0     880    1143 
   Size:        880     263
 
ApoI r'AATT_y

Cuts at:      0     481     523     613     688    1143 
   Size:        481      42      90      75     455

  Fragments arranged by size: 

                 481     455      90      75      42
 
AseI AT'TA_AT

Cuts at:      0    1073    1143 
   Size:       1073      70
 
BamHI G'GATC_C

Cuts at:      0     397    1143 
   Size:        397     746
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     300     523    1034    1143 
   Size:        300     223     511     109

  Fragments arranged by size: 

                 511     300     223     109
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     261     295    1143 
   Size:        261      34     848

  Fragments arranged by size: 

                 848     261      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     337    1143 
   Size:        337     806
 
BclI T'GATC_A

Cuts at:      0     605    1143 
   Size:        605     538
 
BfrBI ATG'CAT

Cuts at:      0     130    1143 
   Size:        130    1013
 
Bme1580I G_kGCm'C

Cuts at:      0     960    1143 
   Size:        960     183
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     954    1143 
   Size:        954     189
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     772    1143 
   Size:        772     371
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     848    1143 
   Size:        848     295
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     930    1143 
   Size:        930     213
 
BsmI GAATG_Cn'

Cuts at:      0      73     228    1143 
   Size:         73     155     915

  Fragments arranged by size: 

                 915     155      73
 
Bsp1286I G_dGCh'C

Cuts at:      0     960    1143 
   Size:        960     183
 
BspHI T'CATG_A

Cuts at:      0     602    1143 
   Size:        602     541
 
BsrBI CCG'CTC

Cuts at:      0     989    1143 
   Size:        989     154
 
BsrDI GCAATG_nn'

Cuts at:      0     786    1143 
   Size:        786     357
 
BsrGI T'GTAC_A

Cuts at:      0     681    1143 
   Size:        681     462
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     729     967    1143 
   Size:        729     238     176

  Fragments arranged by size: 

                 729     238     176
 
BstYI r'GATC_y

Cuts at:      0     397    1143 
   Size:        397     746
 
DraI TTT'AAA

Cuts at:      0     191     326    1143 
   Size:        191     135     817

  Fragments arranged by size: 

                 817     191     135
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     998    1143 
   Size:        203     795     145

  Fragments arranged by size: 

                 795     203     145
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     863    1143 
   Size:        863     280
 
EcoRI G'AATT_C

Cuts at:      0     481    1143 
   Size:        481     662
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     682    1143 
   Size:        682     461
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     250     282     421     453     862     894    1143 
   Size:        250      32     139      32     409      32     249

  Fragments arranged by size: 

                 409     250     249     139      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     425    1143 
   Size:        425     718
 
MslI CAynn'nnrTG

Cuts at:      0      16     391     601    1143 
   Size:         16     375     210     542

  Fragments arranged by size: 

                 542     375     210      16
 
MspA1I CmG'CkG

Cuts at:      0     181    1143 
   Size:        181     962
 
NdeI CA'TA_TG

Cuts at:      0     489    1143 
   Size:        489     654
 
NsiI A_TGCA'T

Cuts at:      0     132    1143 
   Size:        132    1011
 
PfoI T'CCnGG_A

Cuts at:      0     620    1143 
   Size:        620     523
 
PshAI GACnn'nnGTC

Cuts at:      0     893    1143 
   Size:        893     250
 
PvuII CAG'CTG

Cuts at:      0     181    1143 
   Size:        181     962
 
SfcI C'TryA_G

Cuts at:      0      92    1143 
   Size:         92    1051
 
SmlI C'TyrA_G

Cuts at:      0     667     863    1143 
   Size:        667     196     280

  Fragments arranged by size: 

                 667     280     196
 
StyI C'CwwG_G

Cuts at:      0     805    1143 
   Size:        805     338
 
TatI w'GTAC_w

Cuts at:      0     681    1143 
   Size:        681     462
 

 Enzymes that do cut:

     AccI     AcuI    AflII    AlwNI     ApoI     AseI    BamHI     BbsI
     BcgI    BciVI     BclI    BfrBI Bme1580I     BmrI     BplI    BpuEI
     BsaI     BsmI Bsp1286I    BspHI    BsrBI    BsrDI    BsrGI    BstXI
    BstYI     DraI  Eco57MI    EcoNI    EcoRI     FalI    Hin4I     MmeI
     MslI   MspA1I     NdeI     NsiI     PfoI    PshAI    PvuII     SfcI
     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BglI    BglII
     BlpI    BmgBI     BmtI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspMI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI