(Linear) (Six-base) MAPSORT of: KIBB0929.seq  Check: 6788  from: 1  to: 408



KIBB0929.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

AflIII A'CryG_T

Cuts at:      0     348     408 
   Size:        348      60
 
AgeI A'CCGG_T

Cuts at:      0      34     408 
   Size:         34     374
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210     408 
   Size:        210     198
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323     408 
   Size:        323      85
 
BglII A'GATC_T

Cuts at:      0     243     408 
   Size:        243     165
 
BmtI G_CTAG'C

Cuts at:      0     382     408 
   Size:        382      26
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     408 
   Size:        119     289
 
Bpu10I CC'TnA_GC

Cuts at:      0     227     408 
   Size:        227     181
 
BsaAI yAC'GTr

Cuts at:      0     394     408 
   Size:        394      14
 
BsaWI w'CCGG_w

Cuts at:      0      34     408 
   Size:         34     374
 
BsiEI CG_ry'CG

Cuts at:      0      35     408 
   Size:         35     373
 
BsrBI CCG'CTC

Cuts at:      0      76     408 
   Size:         76     332
 
BsrFI r'CCGG_y

Cuts at:      0      34     408 
   Size:         34     374
 
BstYI r'GATC_y

Cuts at:      0     243     408 
   Size:        243     165
 
DraI TTT'AAA

Cuts at:      0     237     408 
   Size:        237     171
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     408 
   Size:        119     289
 
HaeII r_GCGC'y

Cuts at:      0     145     408 
   Size:        145     263
 
NheI G'CTAG_C

Cuts at:      0     378     408 
   Size:        378      30
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     210     408 
   Size:        210     198
 
StuI AGG'CCT

Cuts at:      0     129     295     408 
   Size:        129     166     113

  Fragments arranged by size: 

                 166     129     113
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      63     408 
   Size:         63     345
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      57     408 
   Size:         57     351
 

 Enzymes that do cut:

   AflIII     AgeI    AlwNI     BbsI    BglII     BmtI     BpmI   Bpu10I
    BsaAI    BsaWI    BsiEI    BsrBI    BsrFI    BstYI     DraI  Eco57MI
    HaeII     NheI    PflMI     StuI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI