Restriction map for
pF1KB0668
(Linear) (Six-base) MAPSORT of: KIBB0668.seq Check: 1203 from: 1 to: 3000
KIBB0668.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
Acc65I G'GTAC_C
Cuts at: 0 732 3000
Size: 732 2268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1898 2068 2884 2948 3000
Size: 689 1209 170 816 64 52
Fragments arranged by size:
1209 816 689 170 64 52
AgeI A'CCGG_T
Cuts at: 0 693 3000
Size: 693 2307
AhdI GACnn_n'nnGTC
Cuts at: 0 1776 3000
Size: 1776 1224
AleI CACnn'nnGTG
Cuts at: 0 1534 3000
Size: 1534 1466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 752 3000
Size: 752 2248
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 641 673 3000
Size: 641 32 2327
Fragments arranged by size:
2327 641 32
ApoI r'AATT_y
Cuts at: 0 456 521 1301 1797 2957 3000
Size: 456 65 780 496 1160 43
Fragments arranged by size:
1160 780 496 456 65 43
BamHI G'GATC_C
Cuts at: 0 373 3000
Size: 373 2627
BanI G'GyrC_C
Cuts at: 0 34 732 1136 1234 2302 3000
Size: 34 698 404 98 1068 698
Fragments arranged by size:
1068 698 698 404 98 34
BanII G_rGCy'C
Cuts at: 0 753 3000
Size: 753 2247
BbeI G_GCGC'C
Cuts at: 0 38 2306 3000
Size: 38 2268 694
Fragments arranged by size:
2268 694 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 2197 2258 2672 3000
Size: 2197 61 414 328
Fragments arranged by size:
2197 414 328 61
BciVI GTATCCnnnnn_n'
Cuts at: 0 529 2054 3000
Size: 529 1525 946
Fragments arranged by size:
1525 946 529
BfrBI ATG'CAT
Cuts at: 0 394 3000
Size: 394 2606
Bme1580I G_kGCm'C
Cuts at: 0 58 107 1237 1813 2187 3000
Size: 58 49 1130 576 374 813
Fragments arranged by size:
1130 813 576 374 58 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 109 1005 2682 3000
Size: 109 896 1677 318
Fragments arranged by size:
1677 896 318 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2604 2606 3000
Size: 2604 2 394
Fragments arranged by size:
2604 394 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 3000
Size: 662 2338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1307 3000
Size: 1307 1693
BsaI GGTCTCn'nnnn_
Cuts at: 0 2375 3000
Size: 2375 625
BsaHI Gr'CG_yC
Cuts at: 0 35 1526 2303 3000
Size: 35 1491 777 697
Fragments arranged by size:
1491 777 697 35
BsaWI w'CCGG_w
Cuts at: 0 693 918 3000
Size: 693 225 2082
Fragments arranged by size:
2082 693 225
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 63 66 199 1018 1093 1203 2414 3000
Size: 63 3 133 819 75 110 1211 586
Fragments arranged by size:
1211 819 586 133 110 75 63 3
BseYI C'CCAG_C
Cuts at: 0 596 2993 3000
Size: 596 2397 7
Fragments arranged by size:
2397 596 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 3000
Size: 730 2270
BsiHKAI G_wGCw'C
Cuts at: 0 432 2673 3000
Size: 432 2241 327
Fragments arranged by size:
2241 432 327
BsmI GAATG_Cn'
Cuts at: 0 396 3000
Size: 396 2604
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1014 3000
Size: 1014 1986
Bsp1286I G_dGCh'C
Cuts at: 0 58 107 432 753 1237 1813 2187 2673
Size: 58 49 325 321 484 576 374 486
Cuts at: 2673 3000
Size: 327
Fragments arranged by size:
576 486 484 374 327 325 321 58
49
BspEI T'CCGG_A
Cuts at: 0 918 3000
Size: 918 2082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 720 744 3000
Size: 720 24 2256
Fragments arranged by size:
2256 720 24
BsrDI GCAATG_nn'
Cuts at: 0 584 3000
Size: 584 2416
BsrFI r'CCGG_y
Cuts at: 0 693 717 3000
Size: 693 24 2283
Fragments arranged by size:
2283 693 24
BsrGI T'GTAC_A
Cuts at: 0 2032 3000
Size: 2032 968
BstBI TT'CG_AA
Cuts at: 0 412 3000
Size: 412 2588
BstXI CCAn_nnnn'nTGG
Cuts at: 0 559 1229 1502 1536 3000
Size: 559 670 273 34 1464
Fragments arranged by size:
1464 670 559 273 34
BstYI r'GATC_y
Cuts at: 0 373 682 915 2599 3000
Size: 373 309 233 1684 401
Fragments arranged by size:
1684 401 373 309 233
BtgI C'CryG_G
Cuts at: 0 83 3000
Size: 83 2917
BtsI GCAGTG_nn'
Cuts at: 0 273 613 2153 3000
Size: 273 340 1540 847
Fragments arranged by size:
1540 847 340 273
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 159 194 3000
Size: 159 35 2806
Fragments arranged by size:
2806 159 35
EaeI y'GGCC_r
Cuts at: 0 2 80 2153 2714 3000
Size: 2 78 2073 561 286
Fragments arranged by size:
2073 561 286 78 2
EarI CTCTTCn'nnn_
Cuts at: 0 995 1874 2903 3000
Size: 995 879 1029 97
Fragments arranged by size:
1029 995 879 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 689 1898 2068 2884 2948 3000
Size: 662 27 1209 170 816 64 52
Fragments arranged by size:
1209 816 662 170 64 52 27
EcoO109I rG'GnC_Cy
Cuts at: 0 622 3000
Size: 622 2378
EcoRI G'AATT_C
Cuts at: 0 521 3000
Size: 521 2479
HaeII r_GCGC'y
Cuts at: 0 38 954 2306 3000
Size: 38 916 1352 694
Fragments arranged by size:
1352 916 694 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1665 1697 1762 1794 2002 2034 3000
Size: 1665 32 65 32 208 32 966
Fragments arranged by size:
1665 966 208 65 32 32 32
HincII GTy'rAC
Cuts at: 0 1329 3000
Size: 1329 1671
KasI G'GCGC_C
Cuts at: 0 34 2302 3000
Size: 34 2268 698
Fragments arranged by size:
2268 698 34
KpnI G_GTAC'C
Cuts at: 0 736 3000
Size: 736 2264
MfeI C'AATT_G
Cuts at: 0 1169 1649 2375 3000
Size: 1169 480 726 625
Fragments arranged by size:
1169 726 625 480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 1684 2796 3000
Size: 303 1381 1112 204
Fragments arranged by size:
1381 1112 303 204
MscI TGG'CCA
Cuts at: 0 2716 3000
Size: 2716 284
MslI CAynn'nnrTG
Cuts at: 0 1534 1685 3000
Size: 1534 151 1315
Fragments arranged by size:
1534 1315 151
MspA1I CmG'CkG
Cuts at: 0 29 600 3000
Size: 29 571 2400
Fragments arranged by size:
2400 571 29
NarI GG'CG_CC
Cuts at: 0 35 2303 3000
Size: 35 2268 697
Fragments arranged by size:
2268 697 35
NsiI A_TGCA'T
Cuts at: 0 396 3000
Size: 396 2604
NspI r_CATG'y
Cuts at: 0 1690 3000
Size: 1690 1310
PasI CC'CwG_GG
Cuts at: 0 1262 3000
Size: 1262 1738
PflMI CCAn_nnn'nTGG
Cuts at: 0 646 1573 3000
Size: 646 927 1427
Fragments arranged by size:
1427 927 646
PfoI T'CCnGG_A
Cuts at: 0 678 3000
Size: 678 2322
PsiI TTA'TAA
Cuts at: 0 1625 3000
Size: 1625 1375
PstI C_TGCA'G
Cuts at: 0 312 605 3000
Size: 312 293 2395
Fragments arranged by size:
2395 312 293
PvuII CAG'CTG
Cuts at: 0 600 3000
Size: 600 2400
SapI GCTCTTCn'nnn_
Cuts at: 0 1874 2903 3000
Size: 1874 1029 97
Fragments arranged by size:
1874 1029 97
SexAI A'CCwGG_T
Cuts at: 0 423 3000
Size: 423 2577
SfcI C'TryA_G
Cuts at: 0 308 601 3000
Size: 308 293 2399
Fragments arranged by size:
2399 308 293
SfoI GGC'GCC
Cuts at: 0 36 2304 3000
Size: 36 2268 696
Fragments arranged by size:
2268 696 36
SmlI C'TyrA_G
Cuts at: 0 1322 3000
Size: 1322 1678
SphI G_CATG'C
Cuts at: 0 1690 3000
Size: 1690 1310
SspI AAT'ATT
Cuts at: 0 285 1548 1763 1981 2457 3000
Size: 285 1263 215 218 476 543
Fragments arranged by size:
1263 543 476 285 218 215
StuI AGG'CCT
Cuts at: 0 127 3000
Size: 127 2873
StyI C'CwwG_G
Cuts at: 0 198 516 2067 2363 2789 3000
Size: 198 318 1551 296 426 211
Fragments arranged by size:
1551 426 318 296 211 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 989 1507 3000
Size: 136 853 518 1493
Fragments arranged by size:
1493 853 518 136
TatI w'GTAC_w
Cuts at: 0 1027 2032 3000
Size: 1027 1005 968
Fragments arranged by size:
1027 1005 968
XmnI GAAnn'nnTTC
Cuts at: 0 456 3000
Size: 456 2544
Enzymes that do cut:
Acc65I AcuI AgeI AhdI AleI AlfI AloI ApoI
BamHI BanI BanII BbeI BbsI BciVI BfrBI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrFI BsrGI BstBI BstXI BstYI BtgI BtsI
CspCI EaeI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I
HincII KasI KpnI MfeI MmeI MscI MslI MspA1I
NarI NsiI NspI PasI PflMI PfoI PsiI PstI
PvuII SapI SexAI SfcI SfoI SmlI SphI SspI
StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AlwNI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbvCI BcgI BclI BglI BglII BlpI BmgBI BmtI
Bpu10I BsaAI BsaBI BsaXI BsiEI BsiWI BspHI BsrBI
BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HindIII HpaI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI