Restriction map for
pF1KB0477
(Linear) (Six-base) MAPSORT of: KIBB0477.seq Check: 8996 from: 1 to: 1554
KIBB0477.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 988 1554
Size: 988 566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 927 1105 1554
Size: 523 404 178 449
Fragments arranged by size:
523 449 404 178
AflIII A'CryG_T
Cuts at: 0 665 1554
Size: 665 889
AgeI A'CCGG_T
Cuts at: 0 677 1554
Size: 677 877
AleI CACnn'nnGTG
Cuts at: 0 1048 1554
Size: 1048 506
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1175 1207 1554
Size: 1175 32 347
Fragments arranged by size:
1175 347 32
AlwNI CAG_nnn'CTG
Cuts at: 0 285 411 501 980 1075 1101 1554
Size: 285 126 90 479 95 26 453
Fragments arranged by size:
479 453 285 126 95 90 26
ApaI G_GGCC'C
Cuts at: 0 357 1554
Size: 357 1197
ApoI r'AATT_y
Cuts at: 0 845 1554
Size: 845 709
AvaI C'yCGr_G
Cuts at: 0 59 557 605 1016 1554
Size: 59 498 48 411 538
Fragments arranged by size:
538 498 411 59 48
BanI G'GyrC_C
Cuts at: 0 777 1554
Size: 777 777
BanII G_rGCy'C
Cuts at: 0 227 357 708 1093 1554
Size: 227 130 351 385 461
Fragments arranged by size:
461 385 351 227 130
BbvCI CC'TCA_GC
Cuts at: 0 167 286 1554
Size: 167 119 1268
Fragments arranged by size:
1268 167 119
BglI GCCn_nnn'nGGC
Cuts at: 0 361 1554
Size: 361 1193
BglII A'GATC_T
Cuts at: 0 876 1130 1554
Size: 876 254 424
Fragments arranged by size:
876 424 254
Bme1580I G_kGCm'C
Cuts at: 0 357 420 536 1433 1554
Size: 357 63 116 897 121
Fragments arranged by size:
897 357 121 116 63
BmgBI CAC'GTC
Cuts at: 0 531 1554
Size: 531 1023
BmrI ACTGGGnnnn_n'
Cuts at: 0 1195 1554
Size: 1195 359
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 572 1107 1554
Size: 261 311 535 447
Fragments arranged by size:
535 447 311 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 337 722 957 1102 1123 1414 1426
Size: 241 96 385 235 145 21 291 12
Cuts at: 1426 1477 1554
Size: 51 77
Fragments arranged by size:
385 291 241 235 145 96 77 51
21 12
Bpu10I CC'TnA_GC
Cuts at: 0 167 286 1554
Size: 167 119 1268
Fragments arranged by size:
1268 167 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1554
Size: 496 1058
BsaAI yAC'GTr
Cuts at: 0 421 666 1485 1554
Size: 421 245 819 69
Fragments arranged by size:
819 421 245 69
BsaBI GATnn'nnATC
Cuts at: 0 1129 1554
Size: 1129 425
BsaWI w'CCGG_w
Cuts at: 0 119 308 434 677 1554
Size: 119 189 126 243 877
Fragments arranged by size:
877 243 189 126 119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1099 1129 1175 1205 1318 1348 1554
Size: 1099 30 46 30 113 30 206
Fragments arranged by size:
1099 206 113 46 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 1120 1554
Size: 493 627 434
Fragments arranged by size:
627 493 434
BseYI C'CCAG_C
Cuts at: 0 470 1143 1321 1554
Size: 470 673 178 233
Fragments arranged by size:
673 470 233 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 1257 1554
Size: 406 851 297
Fragments arranged by size:
851 406 297
BsiHKAI G_wGCw'C
Cuts at: 0 227 566 1093 1427 1554
Size: 227 339 527 334 127
Fragments arranged by size:
527 339 334 227 127
Bsp1286I G_dGCh'C
Cuts at: 0 227 357 420 536 566 708 1093 1427
Size: 227 130 63 116 30 142 385 334
Cuts at: 1427 1433 1554
Size: 6 121
Fragments arranged by size:
385 334 227 142 130 121 116 63
30 6
BspEI T'CCGG_A
Cuts at: 0 308 434 1554
Size: 308 126 1120
Fragments arranged by size:
1120 308 126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 263 500 698 959 988 1554
Size: 263 237 198 261 29 566
Fragments arranged by size:
566 263 261 237 198 29
BsrBI CCG'CTC
Cuts at: 0 183 1554
Size: 183 1371
BsrFI r'CCGG_y
Cuts at: 0 677 1554
Size: 677 877
BssHII G'CGCG_C
Cuts at: 0 142 1554
Size: 142 1412
BssSI C'ACGA_G
Cuts at: 0 1426 1554
Size: 1426 128
BstEII G'GTnAC_C
Cuts at: 0 1254 1554
Size: 1254 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 376 670 1554
Size: 376 294 884
Fragments arranged by size:
884 376 294
BstYI r'GATC_y
Cuts at: 0 305 431 876 1130 1554
Size: 305 126 445 254 424
Fragments arranged by size:
445 424 305 254 126
BtgI C'CryG_G
Cuts at: 0 11 792 935 1554
Size: 11 781 143 619
Fragments arranged by size:
781 619 143 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1368 1554
Size: 1368 186
BtsI GCAGTG_nn'
Cuts at: 0 353 806 1554
Size: 353 453 748
Fragments arranged by size:
748 453 353
DraIII CAC_nnn'GTG
Cuts at: 0 936 1347 1554
Size: 936 411 207
Fragments arranged by size:
936 411 207
DrdI GACnn_nn'nnGTC
Cuts at: 0 535 955 1554
Size: 535 420 599
Fragments arranged by size:
599 535 420
EaeI y'GGCC_r
Cuts at: 0 423 672 1536 1554
Size: 423 249 864 18
Fragments arranged by size:
864 423 249 18
EarI CTCTTCn'nnn_
Cuts at: 0 197 317 1554
Size: 197 120 1237
Fragments arranged by size:
1237 197 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 742 1554
Size: 265 477 812
Fragments arranged by size:
812 477 265
EcoICRI GAG'CTC
Cuts at: 0 225 1091 1554
Size: 225 866 463
Fragments arranged by size:
866 463 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 337 523 722 927 957 1102 1105
Size: 241 96 186 199 205 30 145 3
Cuts at: 1105 1123 1414 1426 1477 1554
Size: 18 291 12 51 77
Fragments arranged by size:
291 241 205 199 186 145 96 77
51 30 18 12 3
EcoO109I rG'GnC_Cy
Cuts at: 0 354 393 948 1072 1161 1554
Size: 354 39 555 124 89 393
Fragments arranged by size:
555 393 354 124 89 39
FspI TGC'GCA
Cuts at: 0 192 1554
Size: 192 1362
HaeII r_GCGC'y
Cuts at: 0 24 502 657 1554
Size: 24 478 155 897
Fragments arranged by size:
897 478 155 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 1099 1131 1554
Size: 56 32 1011 32 423
Fragments arranged by size:
1011 423 56 32 32
HincII GTy'rAC
Cuts at: 0 729 1554
Size: 729 825
HindIII A'AGCT_T
Cuts at: 0 745 1554
Size: 745 809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 555 624 1292 1554
Size: 101 454 69 668 262
Fragments arranged by size:
668 454 262 101 69
MscI TGG'CCA
Cuts at: 0 674 1538 1554
Size: 674 864 16
Fragments arranged by size:
864 674 16
MslI CAynn'nnrTG
Cuts at: 0 769 775 1048 1554
Size: 769 6 273 506
Fragments arranged by size:
769 506 273 6
MspA1I CmG'CkG
Cuts at: 0 171 294 334 794 898 1098 1554
Size: 171 123 40 460 104 200 456
Fragments arranged by size:
460 456 200 171 123 104 40
NcoI C'CATG_G
Cuts at: 0 11 1554
Size: 11 1543
NspI r_CATG'y
Cuts at: 0 197 1554
Size: 197 1357
PasI CC'CwG_GG
Cuts at: 0 461 1075 1554
Size: 461 614 479
Fragments arranged by size:
614 479 461
PflMI CCAn_nnn'nTGG
Cuts at: 0 936 1185 1554
Size: 936 249 369
Fragments arranged by size:
936 369 249
PfoI T'CCnGG_A
Cuts at: 0 1163 1391 1554
Size: 1163 228 163
Fragments arranged by size:
1163 228 163
PmlI CAC'GTG
Cuts at: 0 421 666 1485 1554
Size: 421 245 819 69
Fragments arranged by size:
819 421 245 69
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 1175 1207 1554
Size: 469 32 674 32 347
Fragments arranged by size:
674 469 347 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 393 948 1161 1554
Size: 393 555 213 393
Fragments arranged by size:
555 393 393 213
PspOMI G'GGCC_C
Cuts at: 0 353 1554
Size: 353 1201
PspXI vC'TCGA_Gb
Cuts at: 0 557 1554
Size: 557 997
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 115 147 1554
Size: 115 32 1407
Fragments arranged by size:
1407 115 32
PstI C_TGCA'G
Cuts at: 0 299 497 716 1334 1554
Size: 299 198 219 618 220
Fragments arranged by size:
618 299 220 219 198
PvuII CAG'CTG
Cuts at: 0 171 294 334 1098 1554
Size: 171 123 40 764 456
Fragments arranged by size:
764 456 171 123 40
SacI G_AGCT'C
Cuts at: 0 227 1093 1554
Size: 227 866 461
Fragments arranged by size:
866 461 227
SacII CC_GC'GG
Cuts at: 0 795 1554
Size: 795 759
SbfI CC_TGCA'GG
Cuts at: 0 497 1554
Size: 497 1057
SfcI C'TryA_G
Cuts at: 0 104 153 295 493 712 1330 1554
Size: 104 49 142 198 219 618 224
Fragments arranged by size:
618 224 219 198 142 104 49
SmaI CCC'GGG
Cuts at: 0 61 1018 1554
Size: 61 957 536
Fragments arranged by size:
957 536 61
SmlI C'TyrA_G
Cuts at: 0 475 557 1554
Size: 475 82 997
Fragments arranged by size:
997 475 82
SphI G_CATG'C
Cuts at: 0 197 1554
Size: 197 1357
StuI AGG'CCT
Cuts at: 0 1222 1554
Size: 1222 332
StyI C'CwwG_G
Cuts at: 0 11 88 1046 1554
Size: 11 77 958 508
Fragments arranged by size:
958 508 77 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 539 1192 1330 1554
Size: 539 653 138 224
Fragments arranged by size:
653 539 224 138
TatI w'GTAC_w
Cuts at: 0 1032 1496 1554
Size: 1032 464 58
Fragments arranged by size:
1032 464 58
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 619 1182 1318 1554
Size: 619 563 136 236
Fragments arranged by size:
619 563 236 136
XhoI C'TCGA_G
Cuts at: 0 557 1554
Size: 557 997
XmaI C'CCGG_G
Cuts at: 0 59 1016 1554
Size: 59 957 538
Fragments arranged by size:
957 538 59
Enzymes that do cut:
AarI AcuI AflIII AgeI AleI AloI AlwNI ApaI
ApoI AvaI BanI BanII BbvCI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI BstYI
BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuII SacI SacII SbfI SfcI SmaI SmlI SphI
StuI StyI TaqII TatI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BciVI BclI BfrBI BlpI BmtI BsaI
BsaHI BsiEI BsiWI BsmI BsmBI BspHI BsrDI BsrGI
BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI EagI
EcoNI EcoRI EcoRV FalI FseI FspAI HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PshAI PsiI
PvuI RsrII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XmnI ZraI