Restriction map for
pF1KB0469
(Linear) (Six-base) MAPSORT of: KIBB0469.seq Check: 8610 from: 1 to: 1545
KIBB0469.orf
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
Acc65I G'GTAC_C
Cuts at: 0 420 1545
Size: 420 1125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1321 1351 1545
Size: 239 1082 30 194
Fragments arranged by size:
1082 239 194 30
ApaI G_GGCC'C
Cuts at: 0 88 124 1545
Size: 88 36 1421
Fragments arranged by size:
1421 88 36
ApoI r'AATT_y
Cuts at: 0 392 548 1212 1545
Size: 392 156 664 333
Fragments arranged by size:
664 392 333 156
AseI AT'TA_AT
Cuts at: 0 1118 1545
Size: 1118 427
AvaI C'yCGr_G
Cuts at: 0 8 87 1545
Size: 8 79 1458
Fragments arranged by size:
1458 79 8
BanI G'GyrC_C
Cuts at: 0 43 420 1545
Size: 43 377 1125
Fragments arranged by size:
1125 377 43
BanII G_rGCy'C
Cuts at: 0 88 124 1461 1545
Size: 88 36 1337 84
Fragments arranged by size:
1337 88 84 36
BbeI G_GCGC'C
Cuts at: 0 47 1545
Size: 47 1498
BbsI GAAGACnn'nnnn_
Cuts at: 0 1207 1309 1545
Size: 1207 102 236
Fragments arranged by size:
1207 236 102
BbvCI CC'TCA_GC
Cuts at: 0 314 1073 1545
Size: 314 759 472
Fragments arranged by size:
759 472 314
BclI T'GATC_A
Cuts at: 0 941 1146 1545
Size: 941 205 399
Fragments arranged by size:
941 399 205
BglII A'GATC_T
Cuts at: 0 1034 1545
Size: 1034 511
BlpI GC'TnA_GC
Cuts at: 0 1324 1545
Size: 1324 221
Bme1580I G_kGCm'C
Cuts at: 0 88 124 1545
Size: 88 36 1421
Fragments arranged by size:
1421 88 36
BmgBI CAC'GTC
Cuts at: 0 831 1545
Size: 831 714
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 873 1368 1545
Size: 270 603 495 177
Fragments arranged by size:
603 495 270 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 967 1545
Size: 667 300 578
Fragments arranged by size:
667 578 300
Bpu10I CC'TnA_GC
Cuts at: 0 314 1073 1460 1545
Size: 314 759 387 85
Fragments arranged by size:
759 387 314 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 1545
Size: 1372 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 171 297 790 1331 1545
Size: 171 126 493 541 214
Fragments arranged by size:
541 493 214 171 126
BsaHI Gr'CG_yC
Cuts at: 0 44 1545
Size: 44 1501
BsaWI w'CCGG_w
Cuts at: 0 1535 1545
Size: 1535 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 965 995 1456 1471 1486 1501 1545
Size: 965 30 461 15 15 15 44
Fragments arranged by size:
965 461 44 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1435 1438 1545
Size: 1435 3 107
Fragments arranged by size:
1435 107 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1398 1516 1545
Size: 1398 118 29
Fragments arranged by size:
1398 118 29
BsiEI CG_ry'CG
Cuts at: 0 823 1545
Size: 823 722
BsmBI CGTCTCn'nnnn_
Cuts at: 0 821 1497 1545
Size: 821 676 48
Fragments arranged by size:
821 676 48
Bsp1286I G_dGCh'C
Cuts at: 0 88 124 1461 1545
Size: 88 36 1337 84
Fragments arranged by size:
1337 88 84 36
BspEI T'CCGG_A
Cuts at: 0 1535 1545
Size: 1535 10
BspHI T'CATG_A
Cuts at: 0 482 1545
Size: 482 1063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 315 318 1034 1545
Size: 315 3 716 511
Fragments arranged by size:
716 511 315 3
BsrBI CCG'CTC
Cuts at: 0 149 166 999 1545
Size: 149 17 833 546
Fragments arranged by size:
833 546 149 17
BssSI C'ACGA_G
Cuts at: 0 994 1545
Size: 994 551
BstEII G'GTnAC_C
Cuts at: 0 1411 1545
Size: 1411 134
BstYI r'GATC_y
Cuts at: 0 320 444 1034 1067 1268 1545
Size: 320 124 590 33 201 277
Fragments arranged by size:
590 320 277 201 124 33
Bsu36I CC'TnA_GG
Cuts at: 0 54 212 1343 1545
Size: 54 158 1131 202
Fragments arranged by size:
1131 202 158 54
BtgI C'CryG_G
Cuts at: 0 224 1545
Size: 224 1321
BtsI GCAGTG_nn'
Cuts at: 0 1229 1545
Size: 1229 316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 937 972 1545
Size: 937 35 573
Fragments arranged by size:
937 573 35
DraI TTT'AAA
Cuts at: 0 885 1545
Size: 885 660
EaeI y'GGCC_r
Cuts at: 0 820 1545
Size: 820 725
EagI C'GGCC_G
Cuts at: 0 820 1545
Size: 820 725
EarI CTCTTCn'nnn_
Cuts at: 0 705 1545
Size: 705 840
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 107 1545
Size: 107 1438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 667 967 1321 1351 1545
Size: 239 428 300 354 30 194
Fragments arranged by size:
428 354 300 239 194 30
EcoO109I rG'GnC_Cy
Cuts at: 0 103 217 229 987 1383 1545
Size: 103 114 12 758 396 162
Fragments arranged by size:
758 396 162 114 103 12
EcoRI G'AATT_C
Cuts at: 0 1212 1545
Size: 1212 333
EcoRV GAT'ATC
Cuts at: 0 501 1545
Size: 501 1044
HaeII r_GCGC'y
Cuts at: 0 47 1431 1545
Size: 47 1384 114
Fragments arranged by size:
1384 114 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1239 1271 1545
Size: 1239 32 274
Fragments arranged by size:
1239 274 32
HincII GTy'rAC
Cuts at: 0 1137 1545
Size: 1137 408
KasI G'GCGC_C
Cuts at: 0 43 1545
Size: 43 1502
KpnI G_GTAC'C
Cuts at: 0 424 1545
Size: 424 1121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 1545
Size: 285 1260
MspA1I CmG'CkG
Cuts at: 0 1077 1299 1311 1545
Size: 1077 222 12 234
Fragments arranged by size:
1077 234 222 12
NarI GG'CG_CC
Cuts at: 0 44 1545
Size: 44 1501
NcoI C'CATG_G
Cuts at: 0 224 1545
Size: 224 1321
PasI CC'CwG_GG
Cuts at: 0 1454 1527 1545
Size: 1454 73 18
Fragments arranged by size:
1454 73 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 27 1545
Size: 27 1518
PfoI T'CCnGG_A
Cuts at: 0 644 983 1545
Size: 644 339 562
Fragments arranged by size:
644 562 339
PpuMI rG'GwC_Cy
Cuts at: 0 217 229 987 1383 1545
Size: 217 12 758 396 162
Fragments arranged by size:
758 396 217 162 12
PspOMI G'GGCC_C
Cuts at: 0 84 120 1545
Size: 84 36 1425
Fragments arranged by size:
1425 84 36
PstI C_TGCA'G
Cuts at: 0 1031 1545
Size: 1031 514
PvuII CAG'CTG
Cuts at: 0 1299 1545
Size: 1299 246
SanDI GG'GwC_CC
Cuts at: 0 229 1545
Size: 229 1316
SexAI A'CCwGG_T
Cuts at: 0 180 1545
Size: 180 1365
SfcI C'TryA_G
Cuts at: 0 1027 1545
Size: 1027 518
SfoI GGC'GCC
Cuts at: 0 45 1545
Size: 45 1500
SmaI CCC'GGG
Cuts at: 0 10 89 1545
Size: 10 79 1456
Fragments arranged by size:
1456 79 10
SmlI C'TyrA_G
Cuts at: 0 1387 1545
Size: 1387 158
SrfI GCCC'GGGC
Cuts at: 0 10 89 1545
Size: 10 79 1456
Fragments arranged by size:
1456 79 10
StuI AGG'CCT
Cuts at: 0 436 511 1545
Size: 436 75 1034
Fragments arranged by size:
1034 436 75
StyI C'CwwG_G
Cuts at: 0 80 224 716 1490 1545
Size: 80 144 492 774 55
Fragments arranged by size:
774 492 144 80 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 814 1545
Size: 814 731
TatI w'GTAC_w
Cuts at: 0 844 1545
Size: 844 701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 555 587 965 997 1456 1488 1545
Size: 555 32 378 32 459 32 57
Fragments arranged by size:
555 459 378 57 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1257 1545
Size: 1257 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 799 1545
Size: 799 746
XmaI C'CCGG_G
Cuts at: 0 8 87 1545
Size: 8 79 1458
Fragments arranged by size:
1458 79 8
Enzymes that do cut:
Acc65I AcuI ApaI ApoI AseI AvaI BanI BanII
BbeI BbsI BbvCI BclI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BsgI BsiEI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BssSI BstEII BstYI Bsu36I BtgI BtsI CspCI
DraI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV HaeII Hin4I HincII KasI KpnI MmeI MspA1I
NarI NcoI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SanDI SexAI SfcI SfoI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AsiSI
AvrII BaeI BamHI BcgI BciVI BfrBI BglI BmtI
BplI BsaAI BsaBI BseYI BsiHKAI BsiWI BsmI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I BtgZI
ClaI DraIII DrdI EcoICRI EcoNI FalI FseI FspI
FspAI HindIII HpaI MfeI MluI MscI MslI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
Tth111I XhoI XmnI ZraI