Restriction map for
pF1KB0464
(Linear) (Six-base) MAPSORT of: KIBB0464.seq Check: 6136 from: 1 to: 2958
KIBB0464.orf
With 182 enzymes: SgfI *
August 25, 2016 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1611 2958
Size: 1611 1347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1141 1630 2495 2958
Size: 211 930 489 865 463
Fragments arranged by size:
930 865 489 463 211
AflII C'TTAA_G
Cuts at: 0 967 2958
Size: 967 1991
AflIII A'CryG_T
Cuts at: 0 1469 2958
Size: 1469 1489
AgeI A'CCGG_T
Cuts at: 0 932 2958
Size: 932 2026
AhdI GACnn_n'nnGTC
Cuts at: 0 2342 2958
Size: 2342 616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1249 2791 2958
Size: 1249 1542 167
Fragments arranged by size:
1542 1249 167
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1653 1685 2958
Size: 1653 32 1273
Fragments arranged by size:
1653 1273 32
AlwNI CAG_nnn'CTG
Cuts at: 0 882 1402 2958
Size: 882 520 1556
Fragments arranged by size:
1556 882 520
ApaI G_GGCC'C
Cuts at: 0 2001 2958
Size: 2001 957
ApaLI G'TGCA_C
Cuts at: 0 1473 2046 2958
Size: 1473 573 912
Fragments arranged by size:
1473 912 573
ApoI r'AATT_y
Cuts at: 0 139 183 1151 2577 2838 2925 2958
Size: 139 44 968 1426 261 87 33
Fragments arranged by size:
1426 968 261 139 87 44 33
AvrII C'CTAG_G
Cuts at: 0 945 2592 2958
Size: 945 1647 366
Fragments arranged by size:
1647 945 366
BamHI G'GATC_C
Cuts at: 0 529 2958
Size: 529 2429
BanI G'GyrC_C
Cuts at: 0 1174 2155 2958
Size: 1174 981 803
Fragments arranged by size:
1174 981 803
BanII G_rGCy'C
Cuts at: 0 306 1513 2001 2136 2590 2958
Size: 306 1207 488 135 454 368
Fragments arranged by size:
1207 488 454 368 306 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 1295 2416 2693 2958
Size: 515 780 1121 277 265
Fragments arranged by size:
1121 780 515 277 265
BbvCI CC'TCA_GC
Cuts at: 0 904 1633 1904 2958
Size: 904 729 271 1054
Fragments arranged by size:
1054 904 729 271
BclI T'GATC_A
Cuts at: 0 1580 2958
Size: 1580 1378
BglII A'GATC_T
Cuts at: 0 2449 2708 2958
Size: 2449 259 250
Fragments arranged by size:
2449 259 250
BlpI GC'TnA_GC
Cuts at: 0 962 2958
Size: 962 1996
Bme1580I G_kGCm'C
Cuts at: 0 1323 1477 2001 2015 2050 2958
Size: 1323 154 524 14 35 908
Fragments arranged by size:
1323 908 524 154 35 14
BmgBI CAC'GTC
Cuts at: 0 2370 2958
Size: 2370 588
BmrI ACTGGGnnnn_n'
Cuts at: 0 1225 1456 1887 2958
Size: 1225 231 431 1071
Fragments arranged by size:
1225 1071 431 231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 325 1743 2433 2958
Size: 325 1418 690 525
Fragments arranged by size:
1418 690 525 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 853 1344 1588 1713 1801 2150 2958
Size: 376 477 491 244 125 88 349 808
Fragments arranged by size:
808 491 477 376 349 244 125 88
Bpu10I CC'TnA_GC
Cuts at: 0 904 1633 1904 2958
Size: 904 729 271 1054
Fragments arranged by size:
1054 904 729 271
BsaI GGTCTCn'nnnn_
Cuts at: 0 1131 2025 2958
Size: 1131 894 933
Fragments arranged by size:
1131 933 894
BsaAI yAC'GTr
Cuts at: 0 1470 2958
Size: 1470 1488
BsaBI GATnn'nnATC
Cuts at: 0 2078 2558 2958
Size: 2078 480 400
Fragments arranged by size:
2078 480 400
BsaWI w'CCGG_w
Cuts at: 0 489 932 2958
Size: 489 443 2026
Fragments arranged by size:
2026 489 443
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2005 2035 2286 2316 2781 2811 2958
Size: 2005 30 251 30 465 30 147
Fragments arranged by size:
2005 465 251 147 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1374 1642 2013 2958
Size: 1374 268 371 945
Fragments arranged by size:
1374 945 371 268
BseYI C'CCAG_C
Cuts at: 0 640 682 1377 1402 1917 2878 2958
Size: 640 42 695 25 515 961 80
Fragments arranged by size:
961 695 640 515 80 42 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 2036 2958
Size: 1435 601 922
Fragments arranged by size:
1435 922 601
BsiHKAI G_wGCw'C
Cuts at: 0 1477 2050 2070 2958
Size: 1477 573 20 888
Fragments arranged by size:
1477 888 573 20
BsmI GAATG_Cn'
Cuts at: 0 21 1908 2958
Size: 21 1887 1050
Fragments arranged by size:
1887 1050 21
Bsp1286I G_dGCh'C
Cuts at: 0 306 1323 1477 1513 2001 2015 2050 2070
Size: 306 1017 154 36 488 14 35 20
Cuts at: 2070 2136 2590 2958
Size: 66 454 368
Fragments arranged by size:
1017 488 454 368 306 154 66 36
35 20 14
BspEI T'CCGG_A
Cuts at: 0 489 2958
Size: 489 2469
BspHI T'CATG_A
Cuts at: 0 1490 2958
Size: 1490 1468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1611 2958
Size: 1611 1347
BsrDI GCAATG_nn'
Cuts at: 0 2775 2958
Size: 2775 183
BsrFI r'CCGG_y
Cuts at: 0 932 1196 1794 2958
Size: 932 264 598 1164
Fragments arranged by size:
1164 932 598 264
BsrGI T'GTAC_A
Cuts at: 0 2190 2958
Size: 2190 768
BstAPI GCAn_nnn'nTGC
Cuts at: 0 27 2958
Size: 27 2931
BstBI TT'CG_AA
Cuts at: 0 1149 2958
Size: 1149 1809
BstEII G'GTnAC_C
Cuts at: 0 415 2958
Size: 415 2543
BstXI CCAn_nnnn'nTGG
Cuts at: 0 726 1825 2958
Size: 726 1099 1133
Fragments arranged by size:
1133 1099 726
BstYI r'GATC_y
Cuts at: 0 529 591 2449 2522 2708 2958
Size: 529 62 1858 73 186 250
Fragments arranged by size:
1858 529 250 186 73 62
BtgI C'CryG_G
Cuts at: 0 440 2810 2933 2958
Size: 440 2370 123 25
Fragments arranged by size:
2370 440 123 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 491 2958
Size: 491 2467
BtsI GCAGTG_nn'
Cuts at: 0 257 2048 2958
Size: 257 1791 910
Fragments arranged by size:
1791 910 257
ClaI AT'CG_AT
Cuts at: 0 2894 2958
Size: 2894 64
DrdI GACnn_nn'nnGTC
Cuts at: 0 1396 2958
Size: 1396 1562
EaeI y'GGCC_r
Cuts at: 0 2076 2958
Size: 2076 882
EarI CTCTTCn'nnn_
Cuts at: 0 455 1011 1083 1314 1712 2223 2514 2630
Size: 455 556 72 231 398 511 291 116
Cuts at: 2630 2698 2760 2958
Size: 68 62 198
Fragments arranged by size:
556 511 455 398 291 231 198 116
72 68 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2822 2958
Size: 2822 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 376 853 1141 1344 1588 1630 1713
Size: 211 165 477 288 203 244 42 83
Cuts at: 1713 1801 2150 2495 2958
Size: 88 349 345 463
Fragments arranged by size:
477 463 349 345 288 244 211 203
165 88 83 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 566 1169 2958
Size: 566 603 1789
Fragments arranged by size:
1789 603 566
EcoO109I rG'GnC_Cy
Cuts at: 0 83 762 1406 1997 2719 2958
Size: 83 679 644 591 722 239
Fragments arranged by size:
722 679 644 591 239 83
EcoRI G'AATT_C
Cuts at: 0 139 1151 2925 2958
Size: 139 1012 1774 33
Fragments arranged by size:
1774 1012 139 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 982 2958
Size: 982 1976
HaeII r_GCGC'y
Cuts at: 0 2861 2958
Size: 2861 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 558 590 616 648 2328 2360 2653 2685
Size: 558 32 26 32 1680 32 293 32
Cuts at: 2685 2958
Size: 273
Fragments arranged by size:
1680 558 293 273 32 32 32 32
26
HindIII A'AGCT_T
Cuts at: 0 251 784 2088 2784 2958
Size: 251 533 1304 696 174
Fragments arranged by size:
1304 696 533 251 174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1352 2488 2958
Size: 301 1051 1136 470
Fragments arranged by size:
1136 1051 470 301
MscI TGG'CCA
Cuts at: 0 2078 2958
Size: 2078 880
MslI CAynn'nnrTG
Cuts at: 0 697 2648 2958
Size: 697 1951 310
Fragments arranged by size:
1951 697 310
MspA1I CmG'CkG
Cuts at: 0 136 526 882 1686 1733 1917 2958
Size: 136 390 356 804 47 184 1041
Fragments arranged by size:
1041 804 390 356 184 136 47
NcoI C'CATG_G
Cuts at: 0 2810 2933 2958
Size: 2810 123 25
Fragments arranged by size:
2810 123 25
NspI r_CATG'y
Cuts at: 0 706 2958
Size: 706 2252
PasI CC'CwG_GG
Cuts at: 0 2001 2801 2958
Size: 2001 800 157
Fragments arranged by size:
2001 800 157
PflMI CCAn_nnn'nTGG
Cuts at: 0 2816 2958
Size: 2816 142
PfoI T'CCnGG_A
Cuts at: 0 1211 2363 2900 2958
Size: 1211 1152 537 58
Fragments arranged by size:
1211 1152 537 58
PmlI CAC'GTG
Cuts at: 0 1470 2958
Size: 1470 1488
PpuMI rG'GwC_Cy
Cuts at: 0 83 762 1406 2958
Size: 83 679 644 1552
Fragments arranged by size:
1552 679 644 83
PshAI GACnn'nnGTC
Cuts at: 0 2147 2958
Size: 2147 811
PspOMI G'GGCC_C
Cuts at: 0 1997 2958
Size: 1997 961
PstI C_TGCA'G
Cuts at: 0 264 893 2685 2780 2958
Size: 264 629 1792 95 178
Fragments arranged by size:
1792 629 264 178 95
PvuII CAG'CTG
Cuts at: 0 136 882 1733 2958
Size: 136 746 851 1225
Fragments arranged by size:
1225 851 746 136
SanDI GG'GwC_CC
Cuts at: 0 1406 2958
Size: 1406 1552
SapI GCTCTTCn'nnn_
Cuts at: 0 2630 2958
Size: 2630 328
SexAI A'CCwGG_T
Cuts at: 0 419 2958
Size: 419 2539
SfcI C'TryA_G
Cuts at: 0 260 889 2681 2776 2958
Size: 260 629 1792 95 182
Fragments arranged by size:
1792 629 260 182 95
SmlI C'TyrA_G
Cuts at: 0 967 2958
Size: 967 1991
SphI G_CATG'C
Cuts at: 0 706 2958
Size: 706 2252
StyI C'CwwG_G
Cuts at: 0 105 725 758 945 2592 2810 2933 2958
Size: 105 620 33 187 1647 218 123 25
Fragments arranged by size:
1647 620 218 187 123 105 33 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 718 1293 1805 1977 2958
Size: 718 575 512 172 981
Fragments arranged by size:
981 718 575 512 172
TatI w'GTAC_w
Cuts at: 0 800 1866 2190 2958
Size: 800 1066 324 768
Fragments arranged by size:
1066 800 768 324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2005 2037 2781 2813 2958
Size: 2005 32 744 32 145
Fragments arranged by size:
2005 744 145 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1219 2817 2958
Size: 1219 1598 141
Fragments arranged by size:
1598 1219 141
Enzymes that do cut:
AarI AcuI AflII AflIII AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AvrII BamHI BanI BanII
BbsI BbvCI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII MmeI MscI MslI MspA1I NcoI NspI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII
SanDI SapI SexAI SfcI SmlI SphI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AleI AscI AseI
AsiSI AvaI BaeI BbeI BcgI BciVI BfrBI BglI
BmtI BpuEI BsaHI BsiEI BsiWI BsmBI BsrBI BssHII
BssSI BstZ17I Bsu36I CspCI DraI DraIII EagI EcoICRI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI