Restriction map for
pF1KSDB0238
(Linear) (Six-base) MAPSORT of: KIBB0238.seq Check: 4235 from: 1 to: 3771
KIBB0238.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1880 2966 3771
Size: 1880 1086 805
Fragments arranged by size:
1880 1086 805
Acc65I G'GTAC_C
Cuts at: 0 2963 3771
Size: 2963 808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1390 1555 2715 2962 3117 3771
Size: 1390 165 1160 247 155 654
Fragments arranged by size:
1390 1160 654 247 165 155
AflIII A'CryG_T
Cuts at: 0 20 2330 3771
Size: 20 2310 1441
Fragments arranged by size:
2310 1441 20
AleI CACnn'nnGTG
Cuts at: 0 1198 2341 3478 3771
Size: 1198 1143 1137 293
Fragments arranged by size:
1198 1143 1137 293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 544 3771
Size: 544 3227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1406 1438 3268 3300 3637 3655 3669 3687
Size: 1406 32 1830 32 337 18 14 18
Cuts at: 3687 3771
Size: 84
Fragments arranged by size:
1830 1406 337 84 32 32 18 18
14
AlwNI CAG_nnn'CTG
Cuts at: 0 47 897 1380 3771
Size: 47 850 483 2391
Fragments arranged by size:
2391 850 483 47
ApaI G_GGCC'C
Cuts at: 0 1571 2285 2714 3001 3771
Size: 1571 714 429 287 770
Fragments arranged by size:
1571 770 714 429 287
ApoI r'AATT_y
Cuts at: 0 1951 3771
Size: 1951 1820
AvaI C'yCGr_G
Cuts at: 0 67 2089 2761 3771
Size: 67 2022 672 1010
Fragments arranged by size:
2022 1010 672 67
BamHI G'GATC_C
Cuts at: 0 1109 3771
Size: 1109 2662
BanI G'GyrC_C
Cuts at: 0 128 361 400 643 1159 1721 1842 1971
Size: 128 233 39 243 516 562 121 129
Cuts at: 1971 2261 2616 2963 3290 3771
Size: 290 355 347 327 481
Fragments arranged by size:
562 516 481 355 347 327 290 243
233 129 128 121 39
BanII G_rGCy'C
Cuts at: 0 1235 1571 1834 2099 2285 2399 2714 3001
Size: 1235 336 263 265 186 114 315 287
Cuts at: 3001 3220 3771
Size: 219 551
Fragments arranged by size:
1235 551 336 315 287 265 263 219
186 114
BbeI G_GCGC'C
Cuts at: 0 132 1163 1725 1975 2265 3771
Size: 132 1031 562 250 290 1506
Fragments arranged by size:
1506 1031 562 290 250 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 132 1922 3313 3771
Size: 132 1790 1391 458
Fragments arranged by size:
1790 1391 458 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1332 1366 1563 1597 3771
Size: 1332 34 197 34 2174
Fragments arranged by size:
2174 1332 197 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2533 3771
Size: 2533 1238
BglI GCCn_nnn'nGGC
Cuts at: 0 569 839 2834 3771
Size: 569 270 1995 937
Fragments arranged by size:
1995 937 569 270
BglII A'GATC_T
Cuts at: 0 524 3771
Size: 524 3247
Bme1580I G_kGCm'C
Cuts at: 0 364 405 963 1367 1571 1812 2285 2348
Size: 364 41 558 404 204 241 473 63
Cuts at: 2348 2621 2714 3001 3771
Size: 273 93 287 770
Fragments arranged by size:
770 558 473 404 364 287 273 241
204 93 63 41
BmgBI CAC'GTC
Cuts at: 0 2333 3771
Size: 2333 1438
BmrI ACTGGGnnnn_n'
Cuts at: 0 830 961 1181 1361 3771
Size: 830 131 220 180 2410
Fragments arranged by size:
2410 830 220 180 131
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3353 3771
Size: 3353 418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 726 805 1116 1444 1663 1923 2441 2577
Size: 726 79 311 328 219 260 518 136
Cuts at: 2577 2698 2797 3556 3771
Size: 121 99 759 215
Fragments arranged by size:
759 726 518 328 311 260 219 215
136 121 99 79
Bpu10I CC'TnA_GC
Cuts at: 0 1171 2447 3729 3771
Size: 1171 1276 1282 42
Fragments arranged by size:
1282 1276 1171 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 2014 2460 3771
Size: 148 1866 446 1311
Fragments arranged by size:
1866 1311 446 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 1448 1667 2112 2173 3505 3771
Size: 1448 219 445 61 1332 266
Fragments arranged by size:
1448 1332 445 266 219 61
BsaAI yAC'GTr
Cuts at: 0 21 1613 2201 2853 3771
Size: 21 1592 588 652 918
Fragments arranged by size:
1592 918 652 588 21
BsaHI Gr'CG_yC
Cuts at: 0 129 1160 1722 1972 2262 3771
Size: 129 1031 562 250 290 1509
Fragments arranged by size:
1509 1031 562 290 250 129
BsaWI w'CCGG_w
Cuts at: 0 500 1145 1474 2213 3771
Size: 500 645 329 739 1558
Fragments arranged by size:
1558 739 645 500 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 262 292 1346 1376 2770 2800 2842 2872
Size: 262 30 1054 30 1394 30 42 30
Cuts at: 2872 3109 3139 3202 3232 3268 3298 3771
Size: 237 30 63 30 36 30 473
Fragments arranged by size:
1394 1054 473 262 237 63 42 36
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 25 925 1135 3391 3565 3771
Size: 22 3 900 210 2256 174 206
Fragments arranged by size:
2256 900 210 206 174 22 3
BseYI C'CCAG_C
Cuts at: 0 243 357 1645 2098 3771
Size: 243 114 1288 453 1673
Fragments arranged by size:
1673 1288 453 243 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 509 991 1201 1852 2275 2704 3771
Size: 214 295 482 210 651 423 429 1067
Fragments arranged by size:
1067 651 482 429 423 295 214 210
BsiEI CG_ry'CG
Cuts at: 0 858 1671 2503 3499 3771
Size: 858 813 832 996 272
Fragments arranged by size:
996 858 832 813 272
BsiHKAI G_wGCw'C
Cuts at: 0 596 2843 2858 3220 3771
Size: 596 2247 15 362 551
Fragments arranged by size:
2247 596 551 362 15
BsiWI C'GTAC_G
Cuts at: 0 3015 3771
Size: 3015 756
BsmI GAATG_Cn'
Cuts at: 0 1448 3771
Size: 1448 2323
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3105 3771
Size: 3105 666
Bsp1286I G_dGCh'C
Cuts at: 0 364 405 596 963 1235 1367 1571 1812
Size: 364 41 191 367 272 132 204 241
Cuts at: 1812 1834 2099 2285 2348 2399 2621 2714 2843
Size: 22 265 186 63 51 222 93 129
Cuts at: 2843 2858 3001 3220 3771
Size: 15 143 219 551
Fragments arranged by size:
551 367 364 272 265 241 222 219
204 191 186 143 132 129 93 63
51 41 22 15
BspEI T'CCGG_A
Cuts at: 0 500 3771
Size: 500 3271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1874 1880 2966 3245 3771
Size: 1874 6 1086 279 526
Fragments arranged by size:
1874 1086 526 279 6
BsrBI CCG'CTC
Cuts at: 0 549 2211 3771
Size: 549 1662 1560
Fragments arranged by size:
1662 1560 549
BsrDI GCAATG_nn'
Cuts at: 0 3726 3771
Size: 3726 45
BsrFI r'CCGG_y
Cuts at: 0 1223 1839 2502 2845 3771
Size: 1223 616 663 343 926
Fragments arranged by size:
1223 926 663 616 343
BssSI C'ACGA_G
Cuts at: 0 2276 3771
Size: 2276 1495
BstAPI GCAn_nnn'nTGC
Cuts at: 0 47 3771
Size: 47 3724
BstEII G'GTnAC_C
Cuts at: 0 1089 1200 1602 3771
Size: 1089 111 402 2169
Fragments arranged by size:
2169 1089 402 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1030 1687 2190 3688 3771
Size: 1030 657 503 1498 83
Fragments arranged by size:
1498 1030 657 503 83
BstYI r'GATC_y
Cuts at: 0 62 380 503 524 1109 1280 3047 3260
Size: 62 318 123 21 585 171 1767 213
Cuts at: 3260 3771
Size: 511
Fragments arranged by size:
1767 585 511 318 213 171 123 62
21
Bsu36I CC'TnA_GG
Cuts at: 0 2129 2267 3771
Size: 2129 138 1504
Fragments arranged by size:
2129 1504 138
BtgI C'CryG_G
Cuts at: 0 124 434 983 1846 2535 3758 3771
Size: 124 310 549 863 689 1223 13
Fragments arranged by size:
1223 863 689 549 310 124 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 777 3563 3771
Size: 777 2786 208
Fragments arranged by size:
2786 777 208
BtsI GCAGTG_nn'
Cuts at: 0 963 1325 2263 3235 3615 3771
Size: 963 362 938 972 380 156
Fragments arranged by size:
972 963 938 380 362 156
DraIII CAC_nnn'GTG
Cuts at: 0 90 756 3230 3771
Size: 90 666 2474 541
Fragments arranged by size:
2474 666 541 90
DrdI GACnn_nn'nnGTC
Cuts at: 0 682 1063 3771
Size: 682 381 2708
Fragments arranged by size:
2708 682 381
EaeI y'GGCC_r
Cuts at: 0 392 412 938 1127 1241 1775 2500 2754
Size: 392 20 526 189 114 534 725 254
Cuts at: 2754 3623 3678 3771
Size: 869 55 93
Fragments arranged by size:
869 725 534 526 392 254 189 114
93 55 20
EagI C'GGCC_G
Cuts at: 0 2500 3771
Size: 2500 1271
EarI CTCTTCn'nnn_
Cuts at: 0 233 733 3161 3771
Size: 233 500 2428 610
Fragments arranged by size:
2428 610 500 233
EcoICRI GAG'CTC
Cuts at: 0 3218 3771
Size: 3218 553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 726 805 1116 1390 1444 1555 1663 1923
Size: 726 79 311 274 54 111 108 260
Cuts at: 1923 2441 2577 2698 2715 2797 2962 3117 3556
Size: 518 136 121 17 82 165 155 439
Cuts at: 3556 3771
Size: 215
Fragments arranged by size:
726 518 439 311 274 260 215 165
155 136 121 111 108 82 79 54
17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2515 2875 3771
Size: 2515 360 896
Fragments arranged by size:
2515 896 360
EcoO109I rG'GnC_Cy
Cuts at: 0 1076 1439 1567 1568 2281 2282 2710 2913
Size: 1076 363 128 1 713 1 428 203
Cuts at: 2913 2997 2998 3108 3234 3530 3771
Size: 84 1 110 126 296 241
Fragments arranged by size:
1076 713 428 363 296 241 203 128
126 110 84 1 1 1
HaeII r_GCGC'y
Cuts at: 0 13 132 1163 1360 1725 1975 2265 3771
Size: 13 119 1031 197 365 250 290 1506
Fragments arranged by size:
1506 1031 365 290 250 197 119 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 2770 2802 3514 3546 3771
Size: 595 32 2143 32 712 32 225
Fragments arranged by size:
2143 712 595 225 32 32 32
HincII GTy'rAC
Cuts at: 0 2373 3771
Size: 2373 1398
KasI G'GCGC_C
Cuts at: 0 128 1159 1721 1971 2261 3771
Size: 128 1031 562 250 290 1510
Fragments arranged by size:
1510 1031 562 290 250 128
KpnI G_GTAC'C
Cuts at: 0 2967 3771
Size: 2967 804
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 1886 2351 3771
Size: 626 1260 465 1420
Fragments arranged by size:
1420 1260 626 465
MscI TGG'CCA
Cuts at: 0 414 1129 1243 1777 2756 3680 3771
Size: 414 715 114 534 979 924 91
Fragments arranged by size:
979 924 715 534 414 114 91
MslI CAynn'nnrTG
Cuts at: 0 367 1028 1198 2188 2341 3478 3771
Size: 367 661 170 990 153 1137 293
Fragments arranged by size:
1137 990 661 367 293 170 153
MspA1I CmG'CkG
Cuts at: 0 203 338 894 932 1269 1769 2537 3079
Size: 203 135 556 38 337 500 768 542
Cuts at: 3079 3771
Size: 692
Fragments arranged by size:
768 692 556 542 500 337 203 135
38
NarI GG'CG_CC
Cuts at: 0 129 1160 1722 1972 2262 3771
Size: 129 1031 562 250 290 1509
Fragments arranged by size:
1509 1031 562 290 250 129
NspI r_CATG'y
Cuts at: 0 970 3771
Size: 970 2801
PasI CC'CwG_GG
Cuts at: 0 1443 1834 3603 3771
Size: 1443 391 1769 168
Fragments arranged by size:
1769 1443 391 168
PflMI CCAn_nnn'nTGG
Cuts at: 0 93 756 824 1740 2613 2871 3771
Size: 93 663 68 916 873 258 900
Fragments arranged by size:
916 900 873 663 258 93 68
PfoI T'CCnGG_A
Cuts at: 0 782 1679 1900 3771
Size: 782 897 221 1871
Fragments arranged by size:
1871 897 782 221
PmlI CAC'GTG
Cuts at: 0 1613 2201 3771
Size: 1613 588 1570
Fragments arranged by size:
1613 1570 588
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2002 2034 3268 3300 3349 3381 3771
Size: 2002 32 1234 32 49 32 390
Fragments arranged by size:
2002 1234 390 49 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1439 2913 3108 3234 3530 3771
Size: 1439 1474 195 126 296 241
Fragments arranged by size:
1474 1439 296 241 195 126
PshAI GACnn'nnGTC
Cuts at: 0 2119 3771
Size: 2119 1652
PspOMI G'GGCC_C
Cuts at: 0 1567 2281 2710 2997 3771
Size: 1567 714 429 287 774
Fragments arranged by size:
1567 774 714 429 287
PstI C_TGCA'G
Cuts at: 0 43 893 1914 3242 3771
Size: 43 850 1021 1328 529
Fragments arranged by size:
1328 1021 850 529 43
PvuII CAG'CTG
Cuts at: 0 894 1269 1769 3771
Size: 894 375 500 2002
Fragments arranged by size:
2002 894 500 375
SacI G_AGCT'C
Cuts at: 0 3220 3771
Size: 3220 551
SacII CC_GC'GG
Cuts at: 0 2538 3771
Size: 2538 1233
SanDI GG'GwC_CC
Cuts at: 0 1439 2913 3108 3771
Size: 1439 1474 195 663
Fragments arranged by size:
1474 1439 663 195
SapI GCTCTTCn'nnn_
Cuts at: 0 233 733 3771
Size: 233 500 3038
Fragments arranged by size:
3038 500 233
ScaI AGT'ACT
Cuts at: 0 1705 3771
Size: 1705 2066
SexAI A'CCwGG_T
Cuts at: 0 752 3771
Size: 752 3019
SfcI C'TryA_G
Cuts at: 0 39 889 1746 1764 1910 2661 3090 3238
Size: 39 850 857 18 146 751 429 148
Cuts at: 3238 3771
Size: 533
Fragments arranged by size:
857 850 751 533 429 148 146 39
18
SfoI GGC'GCC
Cuts at: 0 130 1161 1723 1973 2263 3771
Size: 130 1031 562 250 290 1508
Fragments arranged by size:
1508 1031 562 290 250 130
SmaI CCC'GGG
Cuts at: 0 2091 3771
Size: 2091 1680
SmlI C'TyrA_G
Cuts at: 0 163 2029 2475 3771
Size: 163 1866 446 1296
Fragments arranged by size:
1866 1296 446 163
SphI G_CATG'C
Cuts at: 0 970 3771
Size: 970 2801
StuI AGG'CCT
Cuts at: 0 1348 1621 2689 3559 3771
Size: 1348 273 1068 870 212
Fragments arranged by size:
1348 1068 870 273 212
StyI C'CwwG_G
Cuts at: 0 233 3113 3158 3771
Size: 233 2880 45 613
Fragments arranged by size:
2880 613 233 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 235 2956 3490 3513 3771
Size: 235 2721 534 23 258
Fragments arranged by size:
2721 534 258 235 23
TatI w'GTAC_w
Cuts at: 0 1703 3524 3683 3771
Size: 1703 1821 159 88
Fragments arranged by size:
1821 1703 159 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 74 106 262 294 1140 1172 1346 1378
Size: 74 32 156 32 846 32 174 32
Cuts at: 1378 2531 2563 3109 3141 3771
Size: 1153 32 546 32 630
Fragments arranged by size:
1153 846 630 546 174 156 74 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 859 3771
Size: 859 2912
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 240 354 750 1091 1194 1440 1785 3771
Size: 240 114 396 341 103 246 345 1986
Fragments arranged by size:
1986 396 345 341 246 240 114 103
XmaI C'CCGG_G
Cuts at: 0 2089 3771
Size: 2089 1682
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII KasI KpnI MmeI MscI MslI
MspA1I NarI NspI PasI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI
SapI ScaI SexAI SfcI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI ApaLI
AscI AseI AsiSI AvrII BaeI BbvCI BclI BfrBI
BlpI BmtI BsaBI BspHI BsrGI BssHII BstBI BstZ17I
ClaI CspCI DraI EciI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI MfeI MluI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PsiI PspXI PsrI PvuI RsrII SalI SbfI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
XbaI XhoI XmnI ZraI