Restriction map for
pF1KB0166
(Linear) (Six-base) MAPSORT of: KIBB0166.seq Check: 7065 from: 1 to: 3468
KIBB0166.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AatII G_ACGT'C
Cuts at: 0 1869 3468
Size: 1869 1599
AccI GT'mk_AC
Cuts at: 0 1697 3468
Size: 1697 1771
Acc65I G'GTAC_C
Cuts at: 0 34 3468
Size: 34 3434
AclI AA'CG_TT
Cuts at: 0 247 3468
Size: 247 3221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 409 890 967 1217 1460 2186 2377
Size: 317 92 481 77 250 243 726 191
Cuts at: 2377 2924 3217 3468
Size: 547 293 251
Fragments arranged by size:
726 547 481 317 293 251 250 243
191 92 77
AfeI AGC'GCT
Cuts at: 0 2659 2998 3468
Size: 2659 339 470
Fragments arranged by size:
2659 470 339
AflII C'TTAA_G
Cuts at: 0 2458 3468
Size: 2458 1010
AleI CACnn'nnGTG
Cuts at: 0 452 3468
Size: 452 3016
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1954 1986 3468
Size: 1954 32 1482
Fragments arranged by size:
1954 1482 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1159 1294 1483 1723 2568 3036 3468
Size: 1159 135 189 240 845 468 432
Fragments arranged by size:
1159 845 468 432 240 189 135
ApaI G_GGCC'C
Cuts at: 0 55 74 3468
Size: 55 19 3394
Fragments arranged by size:
3394 55 19
ApoI r'AATT_y
Cuts at: 0 791 1300 2685 2778 3468
Size: 791 509 1385 93 690
Fragments arranged by size:
1385 791 690 509 93
AseI AT'TA_AT
Cuts at: 0 2822 3468
Size: 2822 646
AvaI C'yCGr_G
Cuts at: 0 30 67 3468
Size: 30 37 3401
Fragments arranged by size:
3401 37 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2608 2641 3468
Size: 2608 33 827
Fragments arranged by size:
2608 827 33
BanI G'GyrC_C
Cuts at: 0 34 1363 3468
Size: 34 1329 2105
Fragments arranged by size:
2105 1329 34
BanII G_rGCy'C
Cuts at: 0 55 74 709 945 1631 3468
Size: 55 19 635 236 686 1837
Fragments arranged by size:
1837 686 635 236 55 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 659 1205 1457 1811 3468
Size: 659 546 252 354 1657
Fragments arranged by size:
1657 659 546 354 252
BciVI GTATCCnnnnn_n'
Cuts at: 0 2088 3468
Size: 2088 1380
BclI T'GATC_A
Cuts at: 0 2174 2679 3468
Size: 2174 505 789
Fragments arranged by size:
2174 789 505
BfrBI ATG'CAT
Cuts at: 0 919 3468
Size: 919 2549
BglI GCCn_nnn'nGGC
Cuts at: 0 741 3468
Size: 741 2727
BglII A'GATC_T
Cuts at: 0 2532 3308 3468
Size: 2532 776 160
Fragments arranged by size:
2532 776 160
BlpI GC'TnA_GC
Cuts at: 0 172 2987 3468
Size: 172 2815 481
Fragments arranged by size:
2815 481 172
Bme1580I G_kGCm'C
Cuts at: 0 55 74 3468
Size: 55 19 3394
Fragments arranged by size:
3394 55 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 130 1304 2664 3468
Size: 130 1174 1360 804
Fragments arranged by size:
1360 1174 804 130
BmtI G_CTAG'C
Cuts at: 0 98 3468
Size: 98 3370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 2131 2524 2638 2737 3468
Size: 471 1660 393 114 99 731
Fragments arranged by size:
1660 731 471 393 114 99
Bpu10I CC'TnA_GC
Cuts at: 0 708 3468
Size: 708 2760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1942 2151 2179 2937 3468
Size: 1942 209 28 758 531
Fragments arranged by size:
1942 758 531 209 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 1507 3010 3468
Size: 1507 1503 458
Fragments arranged by size:
1507 1503 458
BsaHI Gr'CG_yC
Cuts at: 0 1866 3468
Size: 1866 1602
BsaWI w'CCGG_w
Cuts at: 0 3357 3468
Size: 3357 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1334 1364 1954 1984 3468
Size: 1334 30 590 30 1484
Fragments arranged by size:
1484 1334 590 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2134 2534 3468
Size: 2134 400 934
Fragments arranged by size:
2134 934 400
BseYI C'CCAG_C
Cuts at: 0 6 1716 3468
Size: 6 1710 1752
Fragments arranged by size:
1752 1710 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 3065 3468
Size: 664 2401 403
Fragments arranged by size:
2401 664 403
BsiEI CG_ry'CG
Cuts at: 0 155 159 3468
Size: 155 4 3309
Fragments arranged by size:
3309 155 4
BsiHKAI G_wGCw'C
Cuts at: 0 143 709 1285 1631 3468
Size: 143 566 576 346 1837
Fragments arranged by size:
1837 576 566 346 143
BsmI GAATG_Cn'
Cuts at: 0 3335 3468
Size: 3335 133
Bsp1286I G_dGCh'C
Cuts at: 0 55 74 143 709 945 1285 1631 3468
Size: 55 19 69 566 236 340 346 1837
Fragments arranged by size:
1837 566 346 340 236 69 55 19
BspHI T'CATG_A
Cuts at: 0 2793 3468
Size: 2793 675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3380 3468
Size: 3380 88
BsrFI r'CCGG_y
Cuts at: 0 150 158 3468
Size: 150 8 3310
Fragments arranged by size:
3310 150 8
BstAPI GCAn_nnn'nTGC
Cuts at: 0 732 750 3468
Size: 732 18 2718
Fragments arranged by size:
2718 732 18
BstYI r'GATC_y
Cuts at: 0 253 544 976 2532 3308 3468
Size: 253 291 432 1556 776 160
Fragments arranged by size:
1556 776 432 291 253 160
Bsu36I CC'TnA_GG
Cuts at: 0 489 737 1751 3468
Size: 489 248 1014 1717
Fragments arranged by size:
1717 1014 489 248
BtgI C'CryG_G
Cuts at: 0 22 1729 2290 3468
Size: 22 1707 561 1178
Fragments arranged by size:
1707 1178 561 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3079 3468
Size: 3079 389
BtsI GCAGTG_nn'
Cuts at: 0 647 3468
Size: 647 2821
ClaI AT'CG_AT
Cuts at: 0 2426 3468
Size: 2426 1042
DraI TTT'AAA
Cuts at: 0 3326 3468
Size: 3326 142
DraIII CAC_nnn'GTG
Cuts at: 0 439 3468
Size: 439 3029
EaeI y'GGCC_r
Cuts at: 0 59 156 559 1901 3468
Size: 59 97 403 1342 1567
Fragments arranged by size:
1567 1342 403 97 59
EagI C'GGCC_G
Cuts at: 0 156 3468
Size: 156 3312
EarI CTCTTCn'nnn_
Cuts at: 0 148 266 486 1313 2247 2552 2707 3117
Size: 148 118 220 827 934 305 155 410
Cuts at: 3117 3236 3468
Size: 119 232
Fragments arranged by size:
934 827 410 305 232 220 155 148
119 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3055 3468
Size: 3055 413
EcoICRI GAG'CTC
Cuts at: 0 707 1629 3468
Size: 707 922 1839
Fragments arranged by size:
1839 922 707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 409 471 890 967 1217 1460 2131
Size: 317 92 62 419 77 250 243 671
Cuts at: 2131 2186 2377 2524 2638 2737 2924 3217 3468
Size: 55 191 147 114 99 187 293 251
Fragments arranged by size:
671 419 317 293 251 250 243 191
187 147 114 99 92 77 62 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 736 3468
Size: 736 2732
EcoO109I rG'GnC_Cy
Cuts at: 0 41 51 3468
Size: 41 10 3417
Fragments arranged by size:
3417 41 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 769 3468
Size: 769 2699
HaeII r_GCGC'y
Cuts at: 0 2661 3000 3468
Size: 2661 339 468
Fragments arranged by size:
2661 468 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1197 1229 1236 1268 1803 1835 1890 1901
Size: 1197 32 7 32 535 32 55 11
Cuts at: 1901 1922 1933 2519 2524 2551 2556 3232 3264
Size: 21 11 586 5 27 5 676 32
Cuts at: 3264 3468
Size: 204
Fragments arranged by size:
1197 676 586 535 204 55 32 32
32 32 27 21 11 11 7 5
5
HincII GTy'rAC
Cuts at: 0 1014 1220 1992 3468
Size: 1014 206 772 1476
Fragments arranged by size:
1476 1014 772 206
HindIII A'AGCT_T
Cuts at: 0 852 3149 3396 3468
Size: 852 2297 247 72
Fragments arranged by size:
2297 852 247 72
KpnI G_GTAC'C
Cuts at: 0 38 3468
Size: 38 3430
MfeI C'AATT_G
Cuts at: 0 3275 3468
Size: 3275 193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2066 3143 3468
Size: 2066 1077 325
Fragments arranged by size:
2066 1077 325
MscI TGG'CCA
Cuts at: 0 561 1903 3468
Size: 561 1342 1565
Fragments arranged by size:
1565 1342 561
MslI CAynn'nnrTG
Cuts at: 0 452 3468
Size: 452 3016
MspA1I CmG'CkG
Cuts at: 0 24 57 312 429 2412 2807 3033 3468
Size: 24 33 255 117 1983 395 226 435
Fragments arranged by size:
1983 435 395 255 226 117 33 24
NaeI GCC'GGC
Cuts at: 0 160 3468
Size: 160 3308
NcoI C'CATG_G
Cuts at: 0 2290 3468
Size: 2290 1178
NgoMIV G'CCGG_C
Cuts at: 0 158 3468
Size: 158 3310
NheI G'CTAG_C
Cuts at: 0 94 3468
Size: 94 3374
NsiI A_TGCA'T
Cuts at: 0 921 3468
Size: 921 2547
NspI r_CATG'y
Cuts at: 0 753 919 1930 3468
Size: 753 166 1011 1538
Fragments arranged by size:
1538 1011 753 166
PasI CC'CwG_GG
Cuts at: 0 114 3468
Size: 114 3354
PflMI CCAn_nnn'nTGG
Cuts at: 0 2159 3468
Size: 2159 1309
PfoI T'CCnGG_A
Cuts at: 0 2435 3468
Size: 2435 1033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1954 1986 3468
Size: 1954 32 1482
Fragments arranged by size:
1954 1482 32
PpuMI rG'GwC_Cy
Cuts at: 0 41 3468
Size: 41 3427
PshAI GACnn'nnGTC
Cuts at: 0 1410 3468
Size: 1410 2058
PspOMI G'GGCC_C
Cuts at: 0 51 70 3468
Size: 51 19 3398
Fragments arranged by size:
3398 51 19
PstI C_TGCA'G
Cuts at: 0 126 1591 2058 2210 3468
Size: 126 1465 467 152 1258
Fragments arranged by size:
1465 1258 467 152 126
PvuII CAG'CTG
Cuts at: 0 312 429 2412 2807 3033 3468
Size: 312 117 1983 395 226 435
Fragments arranged by size:
1983 435 395 312 226 117
SacI G_AGCT'C
Cuts at: 0 709 1631 3468
Size: 709 922 1837
Fragments arranged by size:
1837 922 709
SacII CC_GC'GG
Cuts at: 0 25 3468
Size: 25 3443
SanDI GG'GwC_CC
Cuts at: 0 41 3468
Size: 41 3427
SapI GCTCTTCn'nnn_
Cuts at: 0 148 3117 3236 3468
Size: 148 2969 119 232
Fragments arranged by size:
2969 232 148 119
ScaI AGT'ACT
Cuts at: 0 3352 3468
Size: 3352 116
SfcI C'TryA_G
Cuts at: 0 122 1160 1535 1587 2054 2206 2518 3468
Size: 122 1038 375 52 467 152 312 950
Fragments arranged by size:
1038 950 467 375 312 152 122 52
SmaI CCC'GGG
Cuts at: 0 32 3468
Size: 32 3436
SmlI C'TyrA_G
Cuts at: 0 1957 2130 2194 2458 2916 3468
Size: 1957 173 64 264 458 552
Fragments arranged by size:
1957 552 458 264 173 64
SphI G_CATG'C
Cuts at: 0 753 919 1930 3468
Size: 753 166 1011 1538
Fragments arranged by size:
1538 1011 753 166
SspI AAT'ATT
Cuts at: 0 2190 3448 3468
Size: 2190 1258 20
Fragments arranged by size:
2190 1258 20
StyI C'CwwG_G
Cuts at: 0 2290 3468
Size: 2290 1178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2419 3468
Size: 2419 1049
TatI w'GTAC_w
Cuts at: 0 3350 3468
Size: 3350 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 185 217 1772 1804 3468
Size: 185 32 1555 32 1664
Fragments arranged by size:
1664 1555 185 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1987 3468
Size: 1987 1481
XbaI T'CTAG_A
Cuts at: 0 1393 3468
Size: 1393 2075
XmaI C'CCGG_G
Cuts at: 0 30 3468
Size: 30 3438
XmnI GAAnn'nnTTC
Cuts at: 0 774 2285 3468
Size: 774 1511 1183
Fragments arranged by size:
1511 1183 774
ZraI GAC'GTC
Cuts at: 0 1867 3468
Size: 1867 1601
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AleI
AloI AlwNI ApaI ApoI AseI AvaI BaeI BanI
BanII BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrFI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NheI NsiI NspI PasI PflMI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SanDI SapI ScaI SfcI SmaI SmlI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflIII AgeI AhdI AlfI ApaLI AscI AsiSI
AvrII BamHI BbeI BbvCI BcgI BmgBI BplI BsaAI
BsaBI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I CspCI DrdI EcoRI
EcoRV FseI FspI FspAI HpaI KasI MluI NarI
NdeI NotI NruI PacI PciI PmeI PmlI PsiI
PspXI PsrI PvuI RsrII SalI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI
XcmI XhoI