Restriction map for
pF1KB0069
(Linear) (Six-base) MAPSORT of: KIBB0069.seq Check: 551 from: 1 to: 7740
KIBB0069.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 733 7740
Size: 733 7007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1141 1897 2083 2207 2246 2291 2533
Size: 251 890 756 186 124 39 45 242
Cuts at: 2533 2620 4370 4714 5134 6175 6946 7740
Size: 87 1750 344 420 1041 771 794
Fragments arranged by size:
1750 1041 890 794 771 756 420 344
251 242 186 124 87 45 39
AflII C'TTAA_G
Cuts at: 0 4816 5398 7740
Size: 4816 582 2342
Fragments arranged by size:
4816 2342 582
AflIII A'CryG_T
Cuts at: 0 381 904 2560 7740
Size: 381 523 1656 5180
Fragments arranged by size:
5180 1656 523 381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 7735 7740
Size: 7735 5
AlwNI CAG_nnn'CTG
Cuts at: 0 739 1405 1803 4272 5232 6630 7740
Size: 739 666 398 2469 960 1398 1110
Fragments arranged by size:
2469 1398 1110 960 739 666 398
ApoI r'AATT_y
Cuts at: 0 370 693 869 879 1365 1606 2377 2385
Size: 370 323 176 10 486 241 771 8
Cuts at: 2385 2491 2882 2904 2944 3408 3931 5361 5532
Size: 106 391 22 40 464 523 1430 171
Cuts at: 5532 5538 5762 5783 6022 6130 6214 6359 6406
Size: 6 224 21 239 108 84 145 47
Cuts at: 6406 6782 6798 7066 7301 7446 7524 7740
Size: 376 16 268 235 145 78 216
Fragments arranged by size:
1430 771 523 486 464 391 376 370
323 268 241 239 235 224 216 176
171 145 145 108 106 84 78 47
40 22 21 16 10 8 6
AseI AT'TA_AT
Cuts at: 0 2864 3395 4403 7740
Size: 2864 531 1008 3337
Fragments arranged by size:
3337 2864 1008 531
AvaI C'yCGr_G
Cuts at: 0 4639 7740
Size: 4639 3101
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 914 947 7160 7193 7740
Size: 914 33 6213 33 547
Fragments arranged by size:
6213 914 547 33 33
BanI G'GyrC_C
Cuts at: 0 1268 6703 7740
Size: 1268 5435 1037
Fragments arranged by size:
5435 1268 1037
BanII G_rGCy'C
Cuts at: 0 1104 2234 7740
Size: 1104 1130 5506
Fragments arranged by size:
5506 1130 1104
BbsI GAAGACnn'nnnn_
Cuts at: 0 1694 1784 2524 3139 3712 5329 6691 7740
Size: 1694 90 740 615 573 1617 1362 1049
Fragments arranged by size:
1694 1617 1362 1049 740 615 573 90
BbvCI CC'TCA_GC
Cuts at: 0 2200 7199 7740
Size: 2200 4999 541
Fragments arranged by size:
4999 2200 541
BciVI GTATCCnnnnn_n'
Cuts at: 0 693 7740
Size: 693 7047
BclI T'GATC_A
Cuts at: 0 803 2370 5970 7740
Size: 803 1567 3600 1770
Fragments arranged by size:
3600 1770 1567 803
BfrBI ATG'CAT
Cuts at: 0 5109 5268 6831 7740
Size: 5109 159 1563 909
Fragments arranged by size:
5109 1563 909 159
BglII A'GATC_T
Cuts at: 0 4969 5679 7011 7740
Size: 4969 710 1332 729
Fragments arranged by size:
4969 1332 729 710
BlpI GC'TnA_GC
Cuts at: 0 6986 7740
Size: 6986 754
Bme1580I G_kGCm'C
Cuts at: 0 6708 7740
Size: 6708 1032
BmrI ACTGGGnnnn_n'
Cuts at: 0 3766 7740
Size: 3766 3974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1518 5053 5662 7159 7740
Size: 1518 3535 609 1497 581
Fragments arranged by size:
3535 1518 1497 609 581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 911 943 1891 2002 3868 5653 7740
Size: 859 52 32 948 111 1866 1785 2087
Fragments arranged by size:
2087 1866 1785 948 859 111 52 32
Bpu10I CC'TnA_GC
Cuts at: 0 1103 2200 3916 4247 7199 7740
Size: 1103 1097 1716 331 2952 541
Fragments arranged by size:
2952 1716 1103 1097 541 331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 2455 2693 2795 2854 3490 4778 5023
Size: 739 1716 238 102 59 636 1288 245
Cuts at: 5023 5632 6889 7611 7740
Size: 609 1257 722 129
Fragments arranged by size:
1716 1288 1257 739 722 636 609 245
238 129 102 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 3566 4736 4879 5024 5063 5633 7740
Size: 3566 1170 143 145 39 570 2107
Fragments arranged by size:
3566 2107 1170 570 145 143 39
BsaAI yAC'GTr
Cuts at: 0 382 907 2563 7740
Size: 382 525 1656 5177
Fragments arranged by size:
5177 1656 525 382
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 857 887 909 939 4837 4867 7740
Size: 857 30 22 30 3898 30 2873
Fragments arranged by size:
3898 2873 857 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1093 2452 5023 5101 5632 7695 7740
Size: 1093 1359 2571 78 531 2063 45
Fragments arranged by size:
2571 2063 1359 1093 531 78 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 7740
Size: 900 6840
BsiEI CG_ry'CG
Cuts at: 0 43 7740
Size: 43 7697
BsiHKAI G_wGCw'C
Cuts at: 0 2234 5123 5929 7740
Size: 2234 2889 806 1811
Fragments arranged by size:
2889 2234 1811 806
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1087 7740
Size: 1087 6653
Bsp1286I G_dGCh'C
Cuts at: 0 1104 2234 5123 5929 6708 7740
Size: 1104 1130 2889 806 779 1032
Fragments arranged by size:
2889 1130 1104 1032 806 779
BspHI T'CATG_A
Cuts at: 0 330 3780 4224 5556 6143 6912 7740
Size: 330 3450 444 1332 587 769 828
Fragments arranged by size:
3450 1332 828 769 587 444 330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 7740
Size: 733 7007
BsrDI GCAATG_nn'
Cuts at: 0 1115 7740
Size: 1115 6625
BsrGI T'GTAC_A
Cuts at: 0 3360 7245 7740
Size: 3360 3885 495
Fragments arranged by size:
3885 3360 495
BstAPI GCAn_nnn'nTGC
Cuts at: 0 6703 7740
Size: 6703 1037
BstEII G'GTnAC_C
Cuts at: 0 1203 7740
Size: 1203 6537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 7694 7740
Size: 7694 46
BstYI r'GATC_y
Cuts at: 0 670 1059 3767 4496 4969 5229 5679 6318
Size: 670 389 2708 729 473 260 450 639
Cuts at: 6318 6342 7011 7533 7740
Size: 24 669 522 207
Fragments arranged by size:
2708 729 670 669 639 522 473 450
389 260 207 24
Bsu36I CC'TnA_GG
Cuts at: 0 4996 7707 7740
Size: 4996 2711 33
Fragments arranged by size:
4996 2711 33
BtgI C'CryG_G
Cuts at: 0 17 6125 7740
Size: 17 6108 1615
Fragments arranged by size:
6108 1615 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4576 7740
Size: 4576 3164
BtsI GCAGTG_nn'
Cuts at: 0 720 6802 7740
Size: 720 6082 938
Fragments arranged by size:
6082 938 720
ClaI AT'CG_AT
Cuts at: 0 212 7740
Size: 212 7528
DraI TTT'AAA
Cuts at: 0 3413 5537 6666 7740
Size: 3413 2124 1129 1074
Fragments arranged by size:
3413 2124 1129 1074
DraIII CAC_nnn'GTG
Cuts at: 0 1510 7740
Size: 1510 6230
DrdI GACnn_nn'nnGTC
Cuts at: 0 106 7740
Size: 106 7634
EaeI y'GGCC_r
Cuts at: 0 20 40 7740
Size: 20 20 7700
Fragments arranged by size:
7700 20 20
EagI C'GGCC_G
Cuts at: 0 40 7740
Size: 40 7700
EarI CTCTTCn'nnn_
Cuts at: 0 1215 1251 2222 3362 3758 3944 4346 5604
Size: 1215 36 971 1140 396 186 402 1258
Cuts at: 5604 5944 6449 7740
Size: 340 505 1291
Fragments arranged by size:
1291 1258 1215 1140 971 505 402 396
340 186 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 7740
Size: 19 7721
EcoICRI GAG'CTC
Cuts at: 0 2232 7740
Size: 2232 5508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 859 911 943 1141 1891 1897 2002
Size: 251 608 52 32 198 750 6 105
Cuts at: 2002 2083 2207 2246 2291 2533 2620 3868 4370
Size: 81 124 39 45 242 87 1248 502
Cuts at: 4370 4714 5134 5653 6175 6946 7740
Size: 344 420 519 522 771 794
Fragments arranged by size:
1248 794 771 750 608 522 519 502
420 344 251 242 198 124 105 87
81 52 45 39 32 6
EcoO109I rG'GnC_Cy
Cuts at: 0 3275 4725 6627 7690 7740
Size: 3275 1450 1902 1063 50
Fragments arranged by size:
3275 1902 1450 1063 50
EcoRI G'AATT_C
Cuts at: 0 869 879 2377 6022 6130 7446 7740
Size: 869 10 1498 3645 108 1316 294
Fragments arranged by size:
3645 1498 1316 869 294 108 10
EcoRV GAT'ATC
Cuts at: 0 7181 7740
Size: 7181 559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 82 1171 1280 2323 3283 4021 4300 4375
Size: 82 1089 109 1043 960 738 279 75
Cuts at: 4375 6708 7740
Size: 2333 1032
Fragments arranged by size:
2333 1089 1043 1032 960 738 279 109
82 75
HaeII r_GCGC'y
Cuts at: 0 740 7740
Size: 740 7000
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 455 487 788 820 1086 1118 2079 2111
Size: 455 32 301 32 266 32 961 32
Cuts at: 2111 2439 2471 3943 3945 3975 3977 5320 5352
Size: 328 32 1472 2 30 2 1343 32
Cuts at: 5352 5955 5987 6286 6318 6619 6651 7125 7157
Size: 603 32 299 32 301 32 474 32
Cuts at: 7157 7564 7596 7740
Size: 407 32 144
Fragments arranged by size:
1472 1343 961 603 474 455 407 328
301 301 299 266 144 32 32 32
32 32 32 32 32 32 32 32
30 2 2
HincII GTy'rAC
Cuts at: 0 863 2793 4646 4934 6770 7156 7740
Size: 863 1930 1853 288 1836 386 584
Fragments arranged by size:
1930 1853 1836 863 584 386 288
HindIII A'AGCT_T
Cuts at: 0 780 1354 2135 2742 5502 6487 7024 7740
Size: 780 574 781 607 2760 985 537 716
Fragments arranged by size:
2760 985 781 780 716 607 574 537
HpaI GTT'AAC
Cuts at: 0 863 2793 6770 7740
Size: 863 1930 3977 970
Fragments arranged by size:
3977 1930 970 863
MfeI C'AATT_G
Cuts at: 0 1123 3127 7740
Size: 1123 2004 4613
Fragments arranged by size:
4613 2004 1123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1931 3114 7740
Size: 1931 1183 4626
Fragments arranged by size:
4626 1931 1183
MslI CAynn'nnrTG
Cuts at: 0 164 5207 7740
Size: 164 5043 2533
Fragments arranged by size:
5043 2533 164
MspA1I CmG'CkG
Cuts at: 0 45 2598 4026 4828 7238 7740
Size: 45 2553 1428 802 2410 502
Fragments arranged by size:
2553 2410 1428 802 502 45
NcoI C'CATG_G
Cuts at: 0 6125 7740
Size: 6125 1615
NdeI CA'TA_TG
Cuts at: 0 2402 2912 7740
Size: 2402 510 4828
Fragments arranged by size:
4828 2402 510
NotI GC'GGCC_GC
Cuts at: 0 40 7740
Size: 40 7700
NsiI A_TGCA'T
Cuts at: 0 5111 5270 6833 7740
Size: 5111 159 1563 907
Fragments arranged by size:
5111 1563 907 159
NspI r_CATG'y
Cuts at: 0 5109 5268 7740
Size: 5109 159 2472
Fragments arranged by size:
5109 2472 159
PasI CC'CwG_GG
Cuts at: 0 320 7740
Size: 320 7420
PflMI CCAn_nnn'nTGG
Cuts at: 0 1137 6339 6617 7740
Size: 1137 5202 278 1123
Fragments arranged by size:
5202 1137 1123 278
PfoI T'CCnGG_A
Cuts at: 0 2899 4061 7740
Size: 2899 1162 3679
Fragments arranged by size:
3679 2899 1162
PmlI CAC'GTG
Cuts at: 0 2563 7740
Size: 2563 5177
PpuMI rG'GwC_Cy
Cuts at: 0 3275 6627 7690 7740
Size: 3275 3352 1063 50
Fragments arranged by size:
3352 3275 1063 50
PshAI GACnn'nnGTC
Cuts at: 0 4828 7740
Size: 4828 2912
PsiI TTA'TAA
Cuts at: 0 2360 6850 7123 7740
Size: 2360 4490 273 617
Fragments arranged by size:
4490 2360 617 273
PspXI vC'TCGA_Gb
Cuts at: 0 4639 7740
Size: 4639 3101
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3779 3811 7511 7543 7740
Size: 3779 32 3700 32 197
Fragments arranged by size:
3779 3700 197 32 32
PstI C_TGCA'G
Cuts at: 0 744 2678 3018 7740
Size: 744 1934 340 4722
Fragments arranged by size:
4722 1934 744 340
PvuII CAG'CTG
Cuts at: 0 2598 4026 4828 7740
Size: 2598 1428 802 2912
Fragments arranged by size:
2912 2598 1428 802
SacI G_AGCT'C
Cuts at: 0 2234 7740
Size: 2234 5506
SapI GCTCTTCn'nnn_
Cuts at: 0 2222 7740
Size: 2222 5518
ScaI AGT'ACT
Cuts at: 0 972 6419 6959 7740
Size: 972 5447 540 781
Fragments arranged by size:
5447 972 781 540
SfcI C'TryA_G
Cuts at: 0 740 1705 1820 2639 2674 3014 3608 4241
Size: 740 965 115 819 35 340 594 633
Cuts at: 4241 4264 7100 7740
Size: 23 2836 640
Fragments arranged by size:
2836 965 819 740 640 633 594 340
115 35 23
SmlI C'TyrA_G
Cuts at: 0 754 2434 2708 2810 2833 3469 4639 4793
Size: 754 1680 274 102 23 636 1170 154
Cuts at: 4793 4816 5038 5398 5647 6868 7626 7740
Size: 23 222 360 249 1221 758 114
Fragments arranged by size:
1680 1221 1170 758 754 636 360 274
249 222 154 114 102 23 23
SspI AAT'ATT
Cuts at: 0 3153 7740
Size: 3153 4587
StuI AGG'CCT
Cuts at: 0 3614 7637 7740
Size: 3614 4023 103
Fragments arranged by size:
4023 3614 103
StyI C'CwwG_G
Cuts at: 0 884 1272 3875 3968 4601 6125 6692 7693
Size: 884 388 2603 93 633 1524 567 1001
Cuts at: 7693 7740
Size: 47
Fragments arranged by size:
2603 1524 1001 884 633 567 388 93
47
SwaI ATTT'AAAT
Cuts at: 0 5537 7740
Size: 5537 2203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2497 2536 5858 7740
Size: 2497 39 3322 1882
Fragments arranged by size:
3322 2497 1882 39
TatI w'GTAC_w
Cuts at: 0 970 1164 3360 4716 6417 6957 7245 7740
Size: 970 194 2196 1356 1701 540 288 495
Fragments arranged by size:
2196 1701 1356 970 540 495 288 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 857 889 909 941 4837 4869 7740
Size: 857 32 20 32 3896 32 2871
Fragments arranged by size:
3896 2871 857 32 32 32 20
XbaI T'CTAG_A
Cuts at: 0 1617 5929 7740
Size: 1617 4312 1811
Fragments arranged by size:
4312 1811 1617
XhoI C'TCGA_G
Cuts at: 0 4639 7740
Size: 4639 3101
XmnI GAAnn'nnTTC
Cuts at: 0 2276 3932 4283 4579 5313 5387 6538 7479
Size: 2276 1656 351 296 734 74 1151 941
Cuts at: 7479 7740
Size: 261
Fragments arranged by size:
2276 1656 1151 941 734 351 296 261
74
Enzymes that do cut:
AarI AcuI AflII AflIII AlfI AlwNI ApoI AseI
AvaI BaeI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MslI MspA1I NcoI NdeI NotI
NsiI NspI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI PstI PvuII SacI SapI ScaI
SfcI SmlI SspI StuI StyI SwaI TaqII TatI
TstI XbaI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BamHI BbeI
BcgI BglI BmgBI BmtI BsaBI BsaHI BsaWI BseYI
BsiWI BsmI BspEI BsrBI BsrFI BssHII BssSI BstBI
BstZ17I CspCI EcoNI FseI FspI FspAI KasI KpnI
MluI MscI NaeI NarI NgoMIV NheI NruI PacI
PciI PmeI PpiI PspOMI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI Tth111I XcmI XmaI ZraI