Restriction map for
pF1KSDB0064
(Linear) (Six-base) MAPSORT of: KIBB0064.seq Check: 4300 from: 1 to: 2496
KIBB0064.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AclI AA'CG_TT
Cuts at: 0 2021 2496
Size: 2021 475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 2366 2496
Size: 956 1410 130
Fragments arranged by size:
1410 956 130
AflIII A'CryG_T
Cuts at: 0 975 1878 2496
Size: 975 903 618
Fragments arranged by size:
975 903 618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2134 2496
Size: 2134 362
AlwNI CAG_nnn'CTG
Cuts at: 0 2187 2496
Size: 2187 309
ApaI G_GGCC'C
Cuts at: 0 1306 2496
Size: 1306 1190
ApoI r'AATT_y
Cuts at: 0 95 358 1860 1942 1962 2496
Size: 95 263 1502 82 20 534
Fragments arranged by size:
1502 534 263 95 82 20
AseI AT'TA_AT
Cuts at: 0 701 2496
Size: 701 1795
AvaI C'yCGr_G
Cuts at: 0 280 492 1299 2496
Size: 280 212 807 1197
Fragments arranged by size:
1197 807 280 212
BamHI G'GATC_C
Cuts at: 0 802 2496
Size: 802 1694
BanI G'GyrC_C
Cuts at: 0 198 2496
Size: 198 2298
BanII G_rGCy'C
Cuts at: 0 162 1306 1680 2496
Size: 162 1144 374 816
Fragments arranged by size:
1144 816 374 162
BbsI GAAGACnn'nnnn_
Cuts at: 0 127 655 2496
Size: 127 528 1841
Fragments arranged by size:
1841 528 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1740 1774 2496
Size: 1740 34 722
Fragments arranged by size:
1740 722 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1067 1104 2496
Size: 1067 37 1392
Fragments arranged by size:
1392 1067 37
BlpI GC'TnA_GC
Cuts at: 0 1163 2496
Size: 1163 1333
Bme1580I G_kGCm'C
Cuts at: 0 201 1306 2496
Size: 201 1105 1190
Fragments arranged by size:
1190 1105 201
BmgBI CAC'GTC
Cuts at: 0 1881 2496
Size: 1881 615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 566 1040 1412 1460 2203 2496
Size: 542 24 474 372 48 743 293
Fragments arranged by size:
743 542 474 372 293 48 24
Bpu10I CC'TnA_GC
Cuts at: 0 248 1509 1679 2496
Size: 248 1261 170 817
Fragments arranged by size:
1261 817 248 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1575 2496
Size: 1575 921
BsaWI w'CCGG_w
Cuts at: 0 984 1771 2496
Size: 984 787 725
Fragments arranged by size:
984 787 725
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 540 570 1230 1260 1371 1401 1766 1796
Size: 540 30 660 30 111 30 365 30
Cuts at: 1796 2496
Size: 700
Fragments arranged by size:
700 660 540 365 111 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 596 1100 1385 1484 2219 2496
Size: 124 472 504 285 99 735 277
Fragments arranged by size:
735 504 472 285 277 124 99
BseYI C'CCAG_C
Cuts at: 0 1038 1050 1657 1833 2496
Size: 1038 12 607 176 663
Fragments arranged by size:
1038 663 607 176 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2200 2398 2496
Size: 2200 198 98
Fragments arranged by size:
2200 198 98
BsiHKAI G_wGCw'C
Cuts at: 0 1680 2231 2496
Size: 1680 551 265
Fragments arranged by size:
1680 551 265
BsmI GAATG_Cn'
Cuts at: 0 411 2057 2253 2496
Size: 411 1646 196 243
Fragments arranged by size:
1646 411 243 196
Bsp1286I G_dGCh'C
Cuts at: 0 162 201 1306 1680 2231 2496
Size: 162 39 1105 374 551 265
Fragments arranged by size:
1105 551 374 265 162 39
BspEI T'CCGG_A
Cuts at: 0 984 2496
Size: 984 1512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1859 2126 2496
Size: 1859 267 370
Fragments arranged by size:
1859 370 267
BsrBI CCG'CTC
Cuts at: 0 1131 2496
Size: 1131 1365
BsrDI GCAATG_nn'
Cuts at: 0 198 379 1122 2156 2496
Size: 198 181 743 1034 340
Fragments arranged by size:
1034 743 340 198 181
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1359 2243 2496
Size: 1359 884 253
Fragments arranged by size:
1359 884 253
BstYI r'GATC_y
Cuts at: 0 802 2496
Size: 802 1694
BtgI C'CryG_G
Cuts at: 0 1670 2496
Size: 1670 826
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 432 2109 2496
Size: 432 1677 387
Fragments arranged by size:
1677 432 387
BtsI GCAGTG_nn'
Cuts at: 0 760 2090 2231 2496
Size: 760 1330 141 265
Fragments arranged by size:
1330 760 265 141
ClaI AT'CG_AT
Cuts at: 0 2442 2496
Size: 2442 54
DrdI GACnn_nn'nnGTC
Cuts at: 0 2206 2496
Size: 2206 290
EaeI y'GGCC_r
Cuts at: 0 1414 2496
Size: 1414 1082
EcoICRI GAG'CTC
Cuts at: 0 1678 2496
Size: 1678 818
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 566 956 1040 1412 1460 2203 2366
Size: 542 24 390 84 372 48 743 163
Cuts at: 2366 2496
Size: 130
Fragments arranged by size:
743 542 390 372 163 130 84 48
24
EcoO109I rG'GnC_Cy
Cuts at: 0 1060 1078 1087 1096 1303 1654 2496
Size: 1060 18 9 9 207 351 842
Fragments arranged by size:
1060 842 351 207 18 9 9
EcoRI G'AATT_C
Cuts at: 0 95 1942 2496
Size: 95 1847 554
Fragments arranged by size:
1847 554 95
EcoRV GAT'ATC
Cuts at: 0 2286 2496
Size: 2286 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 415 1547 2378 2496
Size: 415 1132 831 118
Fragments arranged by size:
1132 831 415 118
FspI TGC'GCA
Cuts at: 0 2122 2496
Size: 2122 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 462 494 2435 2467 2496
Size: 462 32 1941 32 29
Fragments arranged by size:
1941 462 32 32 29
HincII GTy'rAC
Cuts at: 0 170 728 992 1544 2025 2496
Size: 170 558 264 552 481 471
Fragments arranged by size:
558 552 481 471 264 170
HpaI GTT'AAC
Cuts at: 0 170 728 2496
Size: 170 558 1768
Fragments arranged by size:
1768 558 170
MfeI C'AATT_G
Cuts at: 0 815 2496
Size: 815 1681
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1425 2409 2496
Size: 1425 984 87
Fragments arranged by size:
1425 984 87
MspA1I CmG'CkG
Cuts at: 0 1042 1837 2496
Size: 1042 795 659
Fragments arranged by size:
1042 795 659
NcoI C'CATG_G
Cuts at: 0 1670 2496
Size: 1670 826
NspI r_CATG'y
Cuts at: 0 979 1959 2031 2496
Size: 979 980 72 465
Fragments arranged by size:
980 979 465 72
PasI CC'CwG_GG
Cuts at: 0 1093 2052 2496
Size: 1093 959 444
Fragments arranged by size:
1093 959 444
PciI A'CATG_T
Cuts at: 0 975 2496
Size: 975 1521
PfoI T'CCnGG_A
Cuts at: 0 1428 2496
Size: 1428 1068
PpuMI rG'GwC_Cy
Cuts at: 0 1078 1654 2496
Size: 1078 576 842
Fragments arranged by size:
1078 842 576
PsiI TTA'TAA
Cuts at: 0 568 2018 2496
Size: 568 1450 478
Fragments arranged by size:
1450 568 478
PspOMI G'GGCC_C
Cuts at: 0 1302 2496
Size: 1302 1194
PstI C_TGCA'G
Cuts at: 0 2475 2496
Size: 2475 21
PvuII CAG'CTG
Cuts at: 0 1042 1837 2496
Size: 1042 795 659
Fragments arranged by size:
1042 795 659
SacI G_AGCT'C
Cuts at: 0 1680 2496
Size: 1680 816
SanDI GG'GwC_CC
Cuts at: 0 1654 2496
Size: 1654 842
SfcI C'TryA_G
Cuts at: 0 71 267 416 1266 2471 2496
Size: 71 196 149 850 1205 25
Fragments arranged by size:
1205 850 196 149 71 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1067 1104 2496
Size: 1067 37 1392
Fragments arranged by size:
1392 1067 37
SmaI CCC'GGG
Cuts at: 0 494 2496
Size: 494 2002
SmlI C'TyrA_G
Cuts at: 0 1554 2496
Size: 1554 942
SspI AAT'ATT
Cuts at: 0 2267 2496
Size: 2267 229
StuI AGG'CCT
Cuts at: 0 34 735 2496
Size: 34 701 1761
Fragments arranged by size:
1761 701 34
StyI C'CwwG_G
Cuts at: 0 997 1670 1937 2496
Size: 997 673 267 559
Fragments arranged by size:
997 673 559 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1275 2496
Size: 1275 1221
TatI w'GTAC_w
Cuts at: 0 1119 2496
Size: 1119 1377
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 540 572 1308 1340 1371 1403 1766 1798
Size: 540 32 736 32 31 32 363 32
Cuts at: 1798 2496
Size: 698
Fragments arranged by size:
736 698 540 363 32 32 32 32
31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 879 2240 2496
Size: 525 354 1361 256
Fragments arranged by size:
1361 525 354 256
XmaI C'CCGG_G
Cuts at: 0 492 2496
Size: 492 2004
Enzymes that do cut:
AclI AcuI AflIII AlfI AlwNI ApaI ApoI AseI
AvaI BamHI BanI BanII BbsI BcgI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BstXI BstYI BtgI BtgZI BtsI ClaI DrdI
EaeI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
Hin4I HincII HpaI MfeI MmeI MspA1I NcoI NspI
PasI PciI PfoI PpuMI PsiI PspOMI PstI PvuII
SacI SanDI SfcI SfiI SmaI SmlI SspI StuI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AsiSI AvrII BaeI BbeI
BbvCI BciVI BclI BfrBI BglII BmrI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspHI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I CspCI DraI DraIII EagI EarI EciI EcoNI
FseI FspAI HaeII HindIII KasI KpnI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI XhoI XmnI ZraI