Restriction map for
pF1KB0063
(Linear) (Six-base) MAPSORT of: KIBB0063.seq Check: 9929 from: 1 to: 6603
KIBB0063.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 16 994 1042 6319 6603
Size: 16 978 48 5277 284
Fragments arranged by size:
5277 978 284 48 16
AatII G_ACGT'C
Cuts at: 0 2026 6603
Size: 2026 4577
AccI GT'mk_AC
Cuts at: 0 1163 4517 6603
Size: 1163 3354 2086
Fragments arranged by size:
3354 2086 1163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 483 527 2165 2404 2608 2956 3708
Size: 224 259 44 1638 239 204 348 752
Cuts at: 3708 6322 6555 6603
Size: 2614 233 48
Fragments arranged by size:
2614 1638 752 348 259 239 233 224
204 48 44
AflIII A'CryG_T
Cuts at: 0 784 2636 2700 3195 6603
Size: 784 1852 64 495 3408
Fragments arranged by size:
3408 1852 784 495 64
AgeI A'CCGG_T
Cuts at: 0 5743 6603
Size: 5743 860
AhdI GACnn_n'nnGTC
Cuts at: 0 1546 1810 2022 6603
Size: 1546 264 212 4581
Fragments arranged by size:
4581 1546 264 212
AleI CACnn'nnGTG
Cuts at: 0 1148 1982 6603
Size: 1148 834 4621
Fragments arranged by size:
4621 1148 834
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 437 6603
Size: 437 6166
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1984 2016 2557 2589 6518 6550 6603
Size: 1984 32 541 32 3929 32 53
Fragments arranged by size:
3929 1984 541 53 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 349 671 1511 1697 1790 6443 6603
Size: 349 322 840 186 93 4653 160
Fragments arranged by size:
4653 840 349 322 186 160 93
ApaI G_GGCC'C
Cuts at: 0 2377 5618 5882 6603
Size: 2377 3241 264 721
Fragments arranged by size:
3241 2377 721 264
ApoI r'AATT_y
Cuts at: 0 2155 3031 4720 5169 6157 6603
Size: 2155 876 1689 449 988 446
Fragments arranged by size:
2155 1689 988 876 449 446
AseI AT'TA_AT
Cuts at: 0 4619 6603
Size: 4619 1984
AvaI C'yCGr_G
Cuts at: 0 1815 2369 4212 4219 4351 5569 5729 6603
Size: 1815 554 1843 7 132 1218 160 874
Fragments arranged by size:
1843 1815 1218 874 554 160 132 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5914 5947 6603
Size: 5914 33 656
Fragments arranged by size:
5914 656 33
BamHI G'GATC_C
Cuts at: 0 181 2196 6603
Size: 181 2015 4407
Fragments arranged by size:
4407 2015 181
BanI G'GyrC_C
Cuts at: 0 231 982 5193 6603
Size: 231 751 4211 1410
Fragments arranged by size:
4211 1410 751 231
BanII G_rGCy'C
Cuts at: 0 248 563 938 1217 2377 3674 5134 5618
Size: 248 315 375 279 1160 1297 1460 484
Cuts at: 5618 5629 5839 5882 6215 6603
Size: 11 210 43 333 388
Fragments arranged by size:
1460 1297 1160 484 388 375 333 315
279 248 210 43 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 288 392 929 1022 1487 1859 2915 3022
Size: 288 104 537 93 465 372 1056 107
Cuts at: 3022 4929 6027 6603
Size: 1907 1098 576
Fragments arranged by size:
1907 1098 1056 576 537 465 372 288
107 104 93
BbvCI CC'TCA_GC
Cuts at: 0 2807 3473 4292 6020 6603
Size: 2807 666 819 1728 583
Fragments arranged by size:
2807 1728 819 666 583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 578 612 696 730 802 836 6603
Size: 578 34 84 34 72 34 5767
Fragments arranged by size:
5767 578 84 72 34 34 34
BclI T'GATC_A
Cuts at: 0 2459 6603
Size: 2459 4144
BglI GCCn_nnn'nGGC
Cuts at: 0 815 1745 2122 5408 6603
Size: 815 930 377 3286 1195
Fragments arranged by size:
3286 1195 930 815 377
BlpI GC'TnA_GC
Cuts at: 0 367 6603
Size: 367 6236
Bme1580I G_kGCm'C
Cuts at: 0 764 2377 5196 5237 5618 5882 6603
Size: 764 1613 2819 41 381 264 721
Fragments arranged by size:
2819 1613 764 721 381 264 41
BmgBI CAC'GTC
Cuts at: 0 2523 2701 6603
Size: 2523 178 3902
Fragments arranged by size:
3902 2523 178
BmrI ACTGGGnnnn_n'
Cuts at: 0 180 751 1298 1685 1778 6603
Size: 180 571 547 387 93 4825
Fragments arranged by size:
4825 571 547 387 180 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 397 406 427 1217 2065 2107 2813
Size: 223 174 9 21 790 848 42 706
Cuts at: 2813 3545 4456 4771 5208 6603
Size: 732 911 315 437 1395
Fragments arranged by size:
1395 911 848 790 732 706 437 315
223 174 42 21 9
Bpu10I CC'TnA_GC
Cuts at: 0 2807 3224 3473 4292 5833 6020 6603
Size: 2807 417 249 819 1541 187 583
Fragments arranged by size:
2807 1541 819 583 417 249 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1201 3385 4851 6603
Size: 58 1143 2184 1466 1752
Fragments arranged by size:
2184 1752 1466 1143 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 2287 2525 2653 2986 4390 4800 5041 6552
Size: 2287 238 128 333 1404 410 241 1511
Cuts at: 6552 6603
Size: 51
Fragments arranged by size:
2287 1511 1404 410 333 241 238 128
51
BsaAI yAC'GTr
Cuts at: 0 2129 6603
Size: 2129 4474
BsaHI Gr'CG_yC
Cuts at: 0 2023 6603
Size: 2023 4580
BsaWI w'CCGG_w
Cuts at: 0 2237 4544 4860 5219 5743 6603
Size: 2237 2307 316 359 524 860
Fragments arranged by size:
2307 2237 860 524 359 316
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 877 907 1063 1093 1204 1234 2557 2587
Size: 877 30 156 30 111 30 1323 30
Cuts at: 2587 3497 3527 6603
Size: 910 30 3076
Fragments arranged by size:
3076 1323 910 877 156 111 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 787 1084 1862 2298 3775 5901 6603
Size: 394 393 297 778 436 1477 2126 702
Fragments arranged by size:
2126 1477 778 702 436 394 393 297
BseYI C'CCAG_C
Cuts at: 0 247 664 2416 2649 3257 3530 3803 4047
Size: 247 417 1752 233 608 273 273 244
Cuts at: 4047 4076 4537 6603
Size: 29 461 2066
Fragments arranged by size:
2066 1752 608 461 417 273 273 247
244 233 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1601 2123 2332 3106 3343 3616 3961 4162
Size: 1601 522 209 774 237 273 345 201
Cuts at: 4162 6603
Size: 2441
Fragments arranged by size:
2441 1601 774 522 345 273 237 209
201
BsiEI CG_ry'CG
Cuts at: 0 489 963 1434 6603
Size: 489 474 471 5169
Fragments arranged by size:
5169 489 474 471
BsiHKAI G_wGCw'C
Cuts at: 0 269 779 938 1217 3308 3467 4127 5629
Size: 269 510 159 279 2091 159 660 1502
Cuts at: 5629 6215 6411 6603
Size: 586 196 192
Fragments arranged by size:
2091 1502 660 586 510 279 269 196
192 159 159
BsmI GAATG_Cn'
Cuts at: 0 2102 4665 6603
Size: 2102 2563 1938
Fragments arranged by size:
2563 2102 1938
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1818 1903 6075 6603
Size: 1818 85 4172 528
Fragments arranged by size:
4172 1818 528 85
Bsp1286I G_dGCh'C
Cuts at: 0 248 269 563 764 779 938 1217 2377
Size: 248 21 294 201 15 159 279 1160
Cuts at: 2377 3308 3467 3674 4127 5134 5196 5237 5618
Size: 931 159 207 453 1007 62 41 381
Cuts at: 5618 5629 5839 5882 6215 6411 6603
Size: 11 210 43 333 196 192
Fragments arranged by size:
1160 1007 931 453 381 333 294 279
248 210 207 201 196 192 159 159
62 43 41 21 15 11
BspEI T'CCGG_A
Cuts at: 0 2237 4544 5219 6603
Size: 2237 2307 675 1384
Fragments arranged by size:
2307 2237 1384 675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 994 1042 2145 3671 5759 6233 6319
Size: 16 978 48 1103 1526 2088 474 86
Cuts at: 6319 6603
Size: 284
Fragments arranged by size:
2088 1526 1103 978 474 284 86 48
16
BsrDI GCAATG_nn'
Cuts at: 0 421 681 2207 5465 5966 6603
Size: 421 260 1526 3258 501 637
Fragments arranged by size:
3258 1526 637 501 421 260
BsrFI r'CCGG_y
Cuts at: 0 593 1061 1102 1567 2303 5743 6603
Size: 593 468 41 465 736 3440 860
Fragments arranged by size:
3440 860 736 593 468 465 41
BsrGI T'GTAC_A
Cuts at: 0 1964 6603
Size: 1964 4639
BssSI C'ACGA_G
Cuts at: 0 1741 1978 6603
Size: 1741 237 4625
Fragments arranged by size:
4625 1741 237
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1670 6130 6603
Size: 1670 4460 473
Fragments arranged by size:
4460 1670 473
BstBI TT'CG_AA
Cuts at: 0 716 6603
Size: 716 5887
BstEII G'GTnAC_C
Cuts at: 0 2391 5746 6603
Size: 2391 3355 857
Fragments arranged by size:
3355 2391 857
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1984 2406 2952 3339 5394 6603
Size: 1984 422 546 387 2055 1209
Fragments arranged by size:
2055 1984 1209 546 422 387
BstYI r'GATC_y
Cuts at: 0 181 198 2196 4035 4388 5861 6358 6603
Size: 181 17 1998 1839 353 1473 497 245
Fragments arranged by size:
1998 1839 1473 497 353 245 181 17
Bsu36I CC'TnA_GG
Cuts at: 0 406 2281 3946 4195 5884 6603
Size: 406 1875 1665 249 1689 719
Fragments arranged by size:
1875 1689 1665 719 406 249
BtgI C'CryG_G
Cuts at: 0 870 1242 1608 3705 3929 5126 5258 5579
Size: 870 372 366 2097 224 1197 132 321
Cuts at: 5579 6234 6338 6603
Size: 655 104 265
Fragments arranged by size:
2097 1197 870 655 372 366 321 265
224 132 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 289 842 5322 6603
Size: 289 553 4480 1281
Fragments arranged by size:
4480 1281 553 289
BtsI GCAGTG_nn'
Cuts at: 0 616 774 936 1084 1242 1270 1401 1642
Size: 616 158 162 148 158 28 131 241
Cuts at: 1642 2450 5636 6603
Size: 808 3186 967
Fragments arranged by size:
3186 967 808 616 241 162 158 158
148 131 28
ClaI AT'CG_AT
Cuts at: 0 2489 2546 6603
Size: 2489 57 4057
Fragments arranged by size:
4057 2489 57
DraIII CAC_nnn'GTG
Cuts at: 0 2355 6603
Size: 2355 4248
DrdI GACnn_nn'nnGTC
Cuts at: 0 5485 6603
Size: 5485 1118
EaeI y'GGCC_r
Cuts at: 0 1152 1239 1245 1431 1611 1704 1725 1767
Size: 1152 87 6 186 180 93 21 42
Cuts at: 1767 2123 3026 3518 5447 6603
Size: 356 903 492 1929 1156
Fragments arranged by size:
1929 1156 1152 903 492 356 186 180
93 87 42 21 6
EagI C'GGCC_G
Cuts at: 0 1431 6603
Size: 1431 5172
EarI CTCTTCn'nnn_
Cuts at: 0 122 1117 1901 2092 2380 3884 5617 6603
Size: 122 995 784 191 288 1504 1733 986
Fragments arranged by size:
1733 1504 995 986 784 288 191 122
EcoICRI GAG'CTC
Cuts at: 0 936 1215 5627 6213 6603
Size: 936 279 4412 586 390
Fragments arranged by size:
4412 936 586 390 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 224 397 406 427 483 527 1217
Size: 223 1 173 9 21 56 44 690
Cuts at: 1217 2065 2107 2165 2404 2608 2813 2956 3545
Size: 848 42 58 239 204 205 143 589
Cuts at: 3545 3708 4456 4771 5208 6322 6555 6603
Size: 163 748 315 437 1114 233 48
Fragments arranged by size:
1114 848 748 690 589 437 315 239
233 223 205 204 173 163 143 58
56 48 44 42 21 9 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 452 5372 5448 6603
Size: 452 4920 76 1155
Fragments arranged by size:
4920 1155 452 76
EcoO109I rG'GnC_Cy
Cuts at: 0 69 473 544 1524 2373 2374 2967 3569
Size: 69 404 71 980 849 1 593 602
Cuts at: 3569 4581 5614 5615 5879 6603
Size: 1012 1033 1 264 724
Fragments arranged by size:
1033 1012 980 849 724 602 593 404
264 71 69 1 1
EcoRI G'AATT_C
Cuts at: 0 4720 6157 6603
Size: 4720 1437 446
Fragments arranged by size:
4720 1437 446
EcoRV GAT'ATC
Cuts at: 0 2342 6603
Size: 2342 4261
HaeII r_GCGC'y
Cuts at: 0 2117 6028 6603
Size: 2117 3911 575
Fragments arranged by size:
3911 2117 575
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 692 724 1532 1564 1796 1828
Size: 190 32 470 32 808 32 232 32
Cuts at: 1828 2008 2040 4232 4264 4347 4379 5389 5421
Size: 180 32 2192 32 83 32 1010 32
Cuts at: 5421 5680 5712 5797 5829 5965 5997 6034 6066
Size: 259 32 85 32 136 32 37 32
Cuts at: 6066 6603
Size: 537
Fragments arranged by size:
2192 1010 808 537 470 259 232 190
180 136 85 83 37 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1917 4152 4869 6456 6501 6603
Size: 1917 2235 717 1587 45 102
Fragments arranged by size:
2235 1917 1587 717 102 45
HindIII A'AGCT_T
Cuts at: 0 255 6603
Size: 255 6348
HpaI GTT'AAC
Cuts at: 0 6501 6603
Size: 6501 102
MfeI C'AATT_G
Cuts at: 0 858 5918 6603
Size: 858 5060 685
Fragments arranged by size:
5060 858 685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2933 4637 5678 6603
Size: 2933 1704 1041 925
Fragments arranged by size:
2933 1704 1041 925
MscI TGG'CCA
Cuts at: 0 1241 1706 1769 2125 3028 5449 6603
Size: 1241 465 63 356 903 2421 1154
Fragments arranged by size:
2421 1241 1154 903 465 356 63
MslI CAynn'nnrTG
Cuts at: 0 1148 1982 3319 3592 4138 5341 6603
Size: 1148 834 1337 273 546 1203 1262
Fragments arranged by size:
1337 1262 1203 1148 834 546 273
MspA1I CmG'CkG
Cuts at: 0 18 654 971 1157 1250 1343 1436 1715
Size: 18 636 317 186 93 93 93 279
Cuts at: 1715 1823 1847 3289 3707 4047 4108 4924 5186
Size: 108 24 1442 418 340 61 816 262
Cuts at: 5186 6603
Size: 1417
Fragments arranged by size:
1442 1417 816 636 418 340 317 279
262 186 108 93 93 93 61 24
18
NcoI C'CATG_G
Cuts at: 0 1242 1608 3929 5126 5258 6234 6338 6603
Size: 1242 366 2321 1197 132 976 104 265
Fragments arranged by size:
2321 1242 1197 976 366 265 132 104
NdeI CA'TA_TG
Cuts at: 0 6126 6603
Size: 6126 477
NspI r_CATG'y
Cuts at: 0 788 1045 1452 2640 3199 6603
Size: 788 257 407 1188 559 3404
Fragments arranged by size:
3404 1188 788 559 407 257
PasI CC'CwG_GG
Cuts at: 0 1056 1505 6198 6603
Size: 1056 449 4693 405
Fragments arranged by size:
4693 1056 449 405
PciI A'CATG_T
Cuts at: 0 784 2636 3195 6603
Size: 784 1852 559 3408
Fragments arranged by size:
3408 1852 784 559
PflMI CCAn_nnn'nTGG
Cuts at: 0 141 3078 6603
Size: 141 2937 3525
Fragments arranged by size:
3525 2937 141
PfoI T'CCnGG_A
Cuts at: 0 2036 2084 3869 4369 4642 4915 5536 6603
Size: 2036 48 1785 500 273 273 621 1067
Fragments arranged by size:
2036 1785 1067 621 500 273 273 48
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2557 2589 5268 5300 6603
Size: 2557 32 2679 32 1303
Fragments arranged by size:
2679 2557 1303 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 69 2967 6603
Size: 69 2898 3636
Fragments arranged by size:
3636 2898 69
PshAI GACnn'nnGTC
Cuts at: 0 3651 6603
Size: 3651 2952
PspOMI G'GGCC_C
Cuts at: 0 2373 5614 5878 6603
Size: 2373 3241 264 725
Fragments arranged by size:
3241 2373 725 264
PspXI vC'TCGA_Gb
Cuts at: 0 4219 5569 6603
Size: 4219 1350 1034
Fragments arranged by size:
4219 1350 1034
PstI C_TGCA'G
Cuts at: 0 426 649 742 769 931 1117 1237 1303
Size: 426 223 93 27 162 186 120 66
Cuts at: 1303 1396 1516 2991 6603
Size: 93 120 1475 3612
Fragments arranged by size:
3612 1475 426 223 186 162 120 120
93 93 66 27
PvuII CAG'CTG
Cuts at: 0 18 654 3289 4047 4108 4924 5186 6603
Size: 18 636 2635 758 61 816 262 1417
Fragments arranged by size:
2635 1417 816 758 636 262 61 18
SacI G_AGCT'C
Cuts at: 0 938 1217 5629 6215 6603
Size: 938 279 4412 586 388
Fragments arranged by size:
4412 938 586 388 279
SacII CC_GC'GG
Cuts at: 0 3708 6603
Size: 3708 2895
SapI GCTCTTCn'nnn_
Cuts at: 0 2092 5617 6603
Size: 2092 3525 986
Fragments arranged by size:
3525 2092 986
ScaI AGT'ACT
Cuts at: 0 2062 2572 3580 4283 4503 6603
Size: 2062 510 1008 703 220 2100
Fragments arranged by size:
2100 2062 1008 703 510 220
SexAI A'CCwGG_T
Cuts at: 0 392 2464 3881 6603
Size: 392 2072 1417 2722
Fragments arranged by size:
2722 2072 1417 392
SfcI C'TryA_G
Cuts at: 0 422 480 645 738 765 927 1113 1233
Size: 422 58 165 93 27 162 186 120
Cuts at: 1233 1299 1392 1512 1842 2987 3450 4016 4269
Size: 66 93 120 330 1145 463 566 253
Cuts at: 4269 5111 5817 6603
Size: 842 706 786
Fragments arranged by size:
1145 842 786 706 566 463 422 330
253 186 165 162 120 120 93 93
66 58 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 5408 6603
Size: 5408 1195
SmaI CCC'GGG
Cuts at: 0 2371 4214 5731 6603
Size: 2371 1843 1517 872
Fragments arranged by size:
2371 1843 1517 872
SmlI C'TyrA_G
Cuts at: 0 73 1216 3400 4219 4830 5569 6603
Size: 73 1143 2184 819 611 739 1034
Fragments arranged by size:
2184 1143 1034 819 739 611 73
StuI AGG'CCT
Cuts at: 0 1757 2150 2675 3125 3298 3478 3944 4297
Size: 1757 393 525 450 173 180 466 353
Cuts at: 4297 4763 6603
Size: 466 1840
Fragments arranged by size:
1840 1757 525 466 466 450 393 353
180 173
StyI C'CwwG_G
Cuts at: 0 1242 1608 3929 4809 5126 5258 5444 5942
Size: 1242 366 2321 880 317 132 186 498
Cuts at: 5942 6234 6338 6603
Size: 292 104 265
Fragments arranged by size:
2321 1242 880 498 366 317 292 265
186 132 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1069 1990 3066 3808 4822 5211 5578 5860
Size: 1069 921 1076 742 1014 389 367 282
Cuts at: 5860 6603
Size: 743
Fragments arranged by size:
1076 1069 1014 921 743 742 389 367
282
TatI w'GTAC_w
Cuts at: 0 418 1964 2060 2570 3143 3578 4281 4501
Size: 418 1546 96 510 573 435 703 220
Cuts at: 4501 6603
Size: 2102
Fragments arranged by size:
2102 1546 703 573 510 435 418 220
96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 866 877 898 909 1063 1095 1204 1236
Size: 866 11 21 11 154 32 109 32
Cuts at: 1236 1528 1560 1714 1746 2176 2208 3497 3529
Size: 292 32 154 32 430 32 1289 32
Cuts at: 3529 6056 6088 6315 6347 6603
Size: 2527 32 227 32 256
Fragments arranged by size:
2527 1289 866 430 292 256 227 154
154 109 32 32 32 32 32 32
32 32 21 11 11
XbaI T'CTAG_A
Cuts at: 0 2202 6603
Size: 2202 4401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 459 2403 4431 5205 5265 5472 5872 6603
Size: 459 1944 2028 774 60 207 400 731
Fragments arranged by size:
2028 1944 774 731 459 400 207 60
XhoI C'TCGA_G
Cuts at: 0 4219 5569 6603
Size: 4219 1350 1034
Fragments arranged by size:
4219 1350 1034
XmaI C'CCGG_G
Cuts at: 0 2369 4212 5729 6603
Size: 2369 1843 1517 874
Fragments arranged by size:
2369 1843 1517 874
XmnI GAAnn'nnTTC
Cuts at: 0 257 6603
Size: 257 6346
ZraI GAC'GTC
Cuts at: 0 2024 6603
Size: 2024 4579
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApoI AseI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BclI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NcoI NdeI NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI
SacII SapI ScaI SexAI SfcI SfiI SmaI SmlI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII ApaLI AscI AsiSI AvrII
BbeI BciVI BfrBI BglII BmtI BplI BsaBI BsiWI
BspHI BsrBI BssHII BstZ17I CspCI DraI EciI FalI
FseI FspI FspAI KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PsiI PsrI PvuI RsrII SalI SanDI SbfI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I