Restriction map for
pF1KB0053
(Linear) (Six-base) MAPSORT of: KIBB0053.seq Check: 7498 from: 1 to: 3936
KIBB0053.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 1094 3936
Size: 1094 2842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 922 2162 2567 2939 3269 3776 3936
Size: 653 269 1240 405 372 330 507 160
Fragments arranged by size:
1240 653 507 405 372 330 269 160
AhdI GACnn_n'nnGTC
Cuts at: 0 3243 3936
Size: 3243 693
AleI CACnn'nnGTG
Cuts at: 0 43 3936
Size: 43 3893
AlwNI CAG_nnn'CTG
Cuts at: 0 2839 3100 3865 3936
Size: 2839 261 765 71
Fragments arranged by size:
2839 765 261 71
ApaI G_GGCC'C
Cuts at: 0 175 3936
Size: 175 3761
ApoI r'AATT_y
Cuts at: 0 661 760 952 1008 1122 1207 1419 3028
Size: 661 99 192 56 114 85 212 1609
Cuts at: 3028 3936
Size: 908
Fragments arranged by size:
1609 908 661 212 192 114 99 85
56
BamHI G'GATC_C
Cuts at: 0 3600 3936
Size: 3600 336
BanII G_rGCy'C
Cuts at: 0 175 3233 3292 3936
Size: 175 3058 59 644
Fragments arranged by size:
3058 644 175 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 301 452 802 1490 2150 3119 3936
Size: 301 151 350 688 660 969 817
Fragments arranged by size:
969 817 688 660 350 301 151
BbvCI CC'TCA_GC
Cuts at: 0 1559 3936
Size: 1559 2377
BciVI GTATCCnnnnn_n'
Cuts at: 0 1129 2046 3936
Size: 1129 917 1890
Fragments arranged by size:
1890 1129 917
BclI T'GATC_A
Cuts at: 0 1449 2214 3936
Size: 1449 765 1722
Fragments arranged by size:
1722 1449 765
BglII A'GATC_T
Cuts at: 0 433 1101 3936
Size: 433 668 2835
Fragments arranged by size:
2835 668 433
Bme1580I G_kGCm'C
Cuts at: 0 40 175 3936
Size: 40 135 3761
Fragments arranged by size:
3761 135 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 2933 3614 3936
Size: 2933 681 322
Fragments arranged by size:
2933 681 322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2189 3936
Size: 2189 1747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 456 1601 2318 2948 3226 3554 3936
Size: 383 73 1145 717 630 278 328 382
Fragments arranged by size:
1145 717 630 383 382 328 278 73
Bpu10I CC'TnA_GC
Cuts at: 0 383 1559 3365 3936
Size: 383 1176 1806 571
Fragments arranged by size:
1806 1176 571 383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2159 3276 3595 3936
Size: 2159 1117 319 341
Fragments arranged by size:
2159 1117 341 319
BsaBI GATnn'nnATC
Cuts at: 0 3599 3605 3936
Size: 3599 6 331
Fragments arranged by size:
3599 331 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 1684 1714 3534 3552 3564 3582
Size: 179 30 1475 30 1820 18 12 18
Cuts at: 3582 3771 3801 3936
Size: 189 30 135
Fragments arranged by size:
1820 1475 189 179 135 30 30 30
18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 859 862 1195 1339 1342 1645 1648
Size: 77 782 3 333 144 3 303 3
Cuts at: 1648 1651 1654 1705 2896 2911 2938 3386 3792
Size: 3 3 51 1191 15 27 448 406
Cuts at: 3792 3822 3936
Size: 30 114
Fragments arranged by size:
1191 782 448 406 333 303 144 114
77 51 30 27 15 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 3223 3936
Size: 3223 713
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 3936
Size: 695 3241
BsiHKAI G_wGCw'C
Cuts at: 0 2175 3233 3292 3936
Size: 2175 1058 59 644
Fragments arranged by size:
2175 1058 644 59
BsmBI CGTCTCn'nnnn_
Cuts at: 0 297 3936
Size: 297 3639
Bsp1286I G_dGCh'C
Cuts at: 0 40 175 2175 3233 3292 3936
Size: 40 135 2000 1058 59 644
Fragments arranged by size:
2000 1058 644 135 59 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1887 3259 3936
Size: 1887 1372 677
Fragments arranged by size:
1887 1372 677
BsrDI GCAATG_nn'
Cuts at: 0 1219 1252 3936
Size: 1219 33 2684
Fragments arranged by size:
2684 1219 33
BsrFI r'CCGG_y
Cuts at: 0 3116 3936
Size: 3116 820
BsrGI T'GTAC_A
Cuts at: 0 947 1038 3337 3936
Size: 947 91 2299 599
Fragments arranged by size:
2299 947 599 91
BstBI TT'CG_AA
Cuts at: 0 3174 3936
Size: 3174 762
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1105 3936
Size: 1105 2831
BstYI r'GATC_y
Cuts at: 0 433 1101 2046 3600 3936
Size: 433 668 945 1554 336
Fragments arranged by size:
1554 945 668 433 336
BtsI GCAGTG_nn'
Cuts at: 0 707 831 891 2902 3936
Size: 707 124 60 2011 1034
Fragments arranged by size:
2011 1034 707 124 60
ClaI AT'CG_AT
Cuts at: 0 3627 3936
Size: 3627 309
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3191 3226 3936
Size: 3191 35 710
Fragments arranged by size:
3191 710 35
DraI TTT'AAA
Cuts at: 0 734 2726 3791 3936
Size: 734 1992 1065 145
Fragments arranged by size:
1992 1065 734 145
DraIII CAC_nnn'GTG
Cuts at: 0 2941 3936
Size: 2941 995
EarI CTCTTCn'nnn_
Cuts at: 0 449 836 1622 1649 1682 2486 2522 2586
Size: 449 387 786 27 33 804 36 64
Cuts at: 2586 2915 3161 3840 3936
Size: 329 246 679 96
Fragments arranged by size:
804 786 679 449 387 329 246 96
64 36 33 27
EcoICRI GAG'CTC
Cuts at: 0 3231 3290 3936
Size: 3231 59 646
Fragments arranged by size:
3231 646 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 456 653 922 1601 2162 2318 2567
Size: 383 73 197 269 679 561 156 249
Cuts at: 2567 2939 2948 3226 3269 3554 3776 3936
Size: 372 9 278 43 285 222 160
Fragments arranged by size:
679 561 383 372 285 278 269 249
222 197 160 156 73 43 9
EcoO109I rG'GnC_Cy
Cuts at: 0 6 171 1803 3936
Size: 6 165 1632 2133
Fragments arranged by size:
2133 1632 165 6
EcoRV GAT'ATC
Cuts at: 0 1003 3936
Size: 1003 2933
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 759 2569 3733 3936
Size: 759 1810 1164 203
Fragments arranged by size:
1810 1164 759 203
HaeII r_GCGC'y
Cuts at: 0 1992 3936
Size: 1992 1944
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 448 474 480 1893 1925 2767 2799
Size: 442 6 26 6 1413 32 842 32
Cuts at: 2799 3229 3261 3552 3584 3936
Size: 430 32 291 32 352
Fragments arranged by size:
1413 842 442 430 352 291 32 32
32 32 26 6 6
HincII GTy'rAC
Cuts at: 0 1239 1467 3875 3936
Size: 1239 228 2408 61
Fragments arranged by size:
2408 1239 228 61
HindIII A'AGCT_T
Cuts at: 0 2123 3936
Size: 2123 1813
HpaI GTT'AAC
Cuts at: 0 1239 1467 3875 3936
Size: 1239 228 2408 61
Fragments arranged by size:
2408 1239 228 61
MfeI C'AATT_G
Cuts at: 0 943 3203 3936
Size: 943 2260 733
Fragments arranged by size:
2260 943 733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3644 3936
Size: 3644 292
MslI CAynn'nnrTG
Cuts at: 0 43 3526 3936
Size: 43 3483 410
Fragments arranged by size:
3483 410 43
MspA1I CmG'CkG
Cuts at: 0 1129 3478 3862 3936
Size: 1129 2349 384 74
Fragments arranged by size:
2349 1129 384 74
NaeI GCC'GGC
Cuts at: 0 3118 3936
Size: 3118 818
NgoMIV G'CCGG_C
Cuts at: 0 3116 3936
Size: 3116 820
PflMI CCAn_nnn'nTGG
Cuts at: 0 3040 3936
Size: 3040 896
PfoI T'CCnGG_A
Cuts at: 0 3570 3936
Size: 3570 366
PpuMI rG'GwC_Cy
Cuts at: 0 1803 3936
Size: 1803 2133
PsiI TTA'TAA
Cuts at: 0 1157 3936
Size: 1157 2779
PspOMI G'GGCC_C
Cuts at: 0 171 3936
Size: 171 3765
PstI C_TGCA'G
Cuts at: 0 1134 2856 2909 3936
Size: 1134 1722 53 1027
Fragments arranged by size:
1722 1134 1027 53
PvuII CAG'CTG
Cuts at: 0 1129 3478 3862 3936
Size: 1129 2349 384 74
Fragments arranged by size:
2349 1129 384 74
SacI G_AGCT'C
Cuts at: 0 3233 3292 3936
Size: 3233 59 644
Fragments arranged by size:
3233 644 59
SapI GCTCTTCn'nnn_
Cuts at: 0 2586 3936
Size: 2586 1350
ScaI AGT'ACT
Cuts at: 0 1186 3936
Size: 1186 2750
SfcI C'TryA_G
Cuts at: 0 269 1130 1188 2852 2905 3936
Size: 269 861 58 1664 53 1031
Fragments arranged by size:
1664 1031 861 269 58 53
SmlI C'TyrA_G
Cuts at: 0 2174 3291 3610 3936
Size: 2174 1117 319 326
Fragments arranged by size:
2174 1117 326 319
SpeI A'CTAG_T
Cuts at: 0 700 3936
Size: 700 3236
SspI AAT'ATT
Cuts at: 0 633 1065 1392 3936
Size: 633 432 327 2544
Fragments arranged by size:
2544 633 432 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2952 3936
Size: 2952 984
TatI w'GTAC_w
Cuts at: 0 947 993 1038 1184 3337 3936
Size: 947 46 45 146 2153 599
Fragments arranged by size:
2153 947 599 146 46 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 1684 1716 1820 1852 3936
Size: 179 32 1473 32 104 32 2084
Fragments arranged by size:
2084 1473 179 104 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3564 3936
Size: 3564 372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1732 3936
Size: 1732 2204
XmnI GAAnn'nnTTC
Cuts at: 0 911 2744 3170 3716 3936
Size: 911 1833 426 546 220
Fragments arranged by size:
1833 911 546 426 220
Enzymes that do cut:
AccI AcuI AhdI AleI AlwNI ApaI ApoI BamHI
BanII BbsI BbvCI BciVI BclI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI
BstBI BstXI BstYI BtsI ClaI CspCI DraI DraIII
EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
HincII HindIII HpaI MfeI MmeI MslI MspA1I NaeI
NgoMIV PflMI PfoI PpuMI PsiI PspOMI PstI PvuII
SacI SapI ScaI SfcI SmlI SpeI SspI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BcgI BfrBI BglI BlpI BmgBI
BmtI BsaI BsaAI BsaHI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BssHII BssSI BstAPI BstEII BstZ17I
Bsu36I BtgI BtgZI DrdI EaeI EagI EciI EcoNI
EcoRI FseI FspI FspAI KasI KpnI MluI MscI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI StyI SwaI XbaI XhoI XmaI ZraI