Restriction map for
pF1KSDB0052
(Linear) (Six-base) MAPSORT of: KIBB0052.seq Check: 5330 from: 1 to: 1863
KIBB0052.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 962 1863
Size: 962 901
AatII G_ACGT'C
Cuts at: 0 1847 1863
Size: 1847 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 802 997 1222 1863
Size: 361 441 195 225 641
Fragments arranged by size:
641 441 361 225 195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1281 1756 1863
Size: 1281 475 107
Fragments arranged by size:
1281 475 107
AlwNI CAG_nnn'CTG
Cuts at: 0 725 883 1084 1641 1836 1863
Size: 725 158 201 557 195 27
Fragments arranged by size:
725 557 201 195 158 27
ApoI r'AATT_y
Cuts at: 0 824 1863
Size: 824 1039
AvaI C'yCGr_G
Cuts at: 0 1767 1863
Size: 1767 96
BanI G'GyrC_C
Cuts at: 0 292 950 1668 1863
Size: 292 658 718 195
Fragments arranged by size:
718 658 292 195
BanII G_rGCy'C
Cuts at: 0 107 410 1863
Size: 107 303 1453
Fragments arranged by size:
1453 303 107
BbsI GAAGACnn'nnnn_
Cuts at: 0 1210 1863
Size: 1210 653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 274 308 1863
Size: 274 34 1555
Fragments arranged by size:
1555 274 34
Bme1580I G_kGCm'C
Cuts at: 0 953 1671 1863
Size: 953 718 192
Fragments arranged by size:
953 718 192
BmgBI CAC'GTC
Cuts at: 0 1536 1863
Size: 1536 327
BmrI ACTGGGnnnn_n'
Cuts at: 0 264 1034 1584 1863
Size: 264 770 550 279
Fragments arranged by size:
770 550 279 264
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 345 1863
Size: 345 1518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 131 340 403 1040 1463 1647 1694
Size: 119 12 209 63 637 423 184 47
Cuts at: 1694 1863
Size: 169
Fragments arranged by size:
637 423 209 184 169 119 63 47
12
Bpu10I CC'TnA_GC
Cuts at: 0 1253 1863
Size: 1253 610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1863
Size: 218 1645
BsaI GGTCTCn'nnnn_
Cuts at: 0 244 413 724 1863
Size: 244 169 311 1139
Fragments arranged by size:
1139 311 244 169
BsaBI GATnn'nnATC
Cuts at: 0 1369 1863
Size: 1369 494
BsaHI Gr'CG_yC
Cuts at: 0 1763 1844 1863
Size: 1763 81 19
Fragments arranged by size:
1763 81 19
BsaWI w'CCGG_w
Cuts at: 0 1754 1863
Size: 1754 109
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 1512 1863
Size: 400 1112 351
Fragments arranged by size:
1112 400 351
BseYI C'CCAG_C
Cuts at: 0 195 423 1728 1863
Size: 195 228 1305 135
Fragments arranged by size:
1305 228 195 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 747 844 1762 1863
Size: 282 465 97 918 101
Fragments arranged by size:
918 465 282 101 97
BsiHKAI G_wGCw'C
Cuts at: 0 107 1016 1863
Size: 107 909 847
Fragments arranged by size:
909 847 107
BsmI GAATG_Cn'
Cuts at: 0 1258 1863
Size: 1258 605
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1770 1863
Size: 1770 93
Bsp1286I G_dGCh'C
Cuts at: 0 107 410 953 1016 1671 1863
Size: 107 303 543 63 655 192
Fragments arranged by size:
655 543 303 192 107 63
BspEI T'CCGG_A
Cuts at: 0 1754 1863
Size: 1754 109
BspHI T'CATG_A
Cuts at: 0 1047 1863
Size: 1047 816
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 418 645 962 1863
Size: 418 227 317 901
Fragments arranged by size:
901 418 317 227
BsrBI CCG'CTC
Cuts at: 0 1007 1863
Size: 1007 856
BsrDI GCAATG_nn'
Cuts at: 0 1556 1863
Size: 1556 307
BsrFI r'CCGG_y
Cuts at: 0 301 1863
Size: 301 1562
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 218 686 1863
Size: 123 95 468 1177
Fragments arranged by size:
1177 468 123 95
BstYI r'GATC_y
Cuts at: 0 852 1211 1863
Size: 852 359 652
Fragments arranged by size:
852 652 359
Bsu36I CC'TnA_GG
Cuts at: 0 19 1642 1863
Size: 19 1623 221
Fragments arranged by size:
1623 221 19
BtgI C'CryG_G
Cuts at: 0 653 1850 1863
Size: 653 1197 13
Fragments arranged by size:
1197 653 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 855 1509 1863
Size: 855 654 354
Fragments arranged by size:
855 654 354
DraIII CAC_nnn'GTG
Cuts at: 0 1308 1863
Size: 1308 555
DrdI GACnn_nn'nnGTC
Cuts at: 0 1552 1863
Size: 1552 311
EaeI y'GGCC_r
Cuts at: 0 1310 1863
Size: 1310 553
EarI CTCTTCn'nnn_
Cuts at: 0 411 1055 1323 1863
Size: 411 644 268 540
Fragments arranged by size:
644 540 411 268
EcoICRI GAG'CTC
Cuts at: 0 105 1863
Size: 105 1758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 131 340 361 403 802 997 1040
Size: 119 12 209 21 42 399 195 43
Cuts at: 1040 1222 1463 1647 1694 1863
Size: 182 241 184 47 169
Fragments arranged by size:
399 241 209 195 184 182 169 119
47 43 42 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 385 1863
Size: 385 1478
EcoO109I rG'GnC_Cy
Cuts at: 0 23 137 1278 1695 1863
Size: 23 114 1141 417 168
Fragments arranged by size:
1141 417 168 114 23
EcoRI G'AATT_C
Cuts at: 0 824 1863
Size: 824 1039
FspI TGC'GCA
Cuts at: 0 1599 1863
Size: 1599 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 249 281 1863
Size: 249 32 1582
Fragments arranged by size:
1582 249 32
HincII GTy'rAC
Cuts at: 0 174 1140 1386 1863
Size: 174 966 246 477
Fragments arranged by size:
966 477 246 174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1635 1863
Size: 132 1503 228
Fragments arranged by size:
1503 228 132
MscI TGG'CCA
Cuts at: 0 1312 1863
Size: 1312 551
MslI CAynn'nnrTG
Cuts at: 0 769 1263 1517 1738 1786 1863
Size: 769 494 254 221 48 77
Fragments arranged by size:
769 494 254 221 77 48
MspA1I CmG'CkG
Cuts at: 0 339 699 1863
Size: 339 360 1164
Fragments arranged by size:
1164 360 339
NcoI C'CATG_G
Cuts at: 0 653 1863
Size: 653 1210
PasI CC'CwG_GG
Cuts at: 0 667 931 1863
Size: 667 264 932
Fragments arranged by size:
932 667 264
PflMI CCAn_nnn'nTGG
Cuts at: 0 1308 1836 1863
Size: 1308 528 27
Fragments arranged by size:
1308 528 27
PfoI T'CCnGG_A
Cuts at: 0 1425 1863
Size: 1425 438
PpuMI rG'GwC_Cy
Cuts at: 0 23 137 1278 1863
Size: 23 114 1141 585
Fragments arranged by size:
1141 585 114 23
PstI C_TGCA'G
Cuts at: 0 373 1109 1863
Size: 373 736 754
Fragments arranged by size:
754 736 373
PvuII CAG'CTG
Cuts at: 0 339 1863
Size: 339 1524
SacI G_AGCT'C
Cuts at: 0 107 1863
Size: 107 1756
SanDI GG'GwC_CC
Cuts at: 0 1278 1863
Size: 1278 585
SapI GCTCTTCn'nnn_
Cuts at: 0 1323 1863
Size: 1323 540
SbfI CC_TGCA'GG
Cuts at: 0 1109 1863
Size: 1109 754
ScaI AGT'ACT
Cuts at: 0 328 1863
Size: 328 1535
SexAI A'CCwGG_T
Cuts at: 0 801 1304 1863
Size: 801 503 559
Fragments arranged by size:
801 559 503
SfcI C'TryA_G
Cuts at: 0 369 864 1105 1863
Size: 369 495 241 758
Fragments arranged by size:
758 495 369 241
SmlI C'TyrA_G
Cuts at: 0 233 1863
Size: 233 1630
StuI AGG'CCT
Cuts at: 0 391 982 1111 1684 1863
Size: 391 591 129 573 179
Fragments arranged by size:
591 573 391 179 129
StyI C'CwwG_G
Cuts at: 0 392 524 653 1112 1230 1863
Size: 392 132 129 459 118 633
Fragments arranged by size:
633 459 392 132 129 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1695 1828 1863
Size: 1695 133 35
Fragments arranged by size:
1695 133 35
TatI w'GTAC_w
Cuts at: 0 326 1613 1863
Size: 326 1287 250
Fragments arranged by size:
1287 326 250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 1592 1624 1863
Size: 7 32 1553 32 239
Fragments arranged by size:
1553 239 32 32 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 175 531 1863
Size: 175 356 1332
Fragments arranged by size:
1332 356 175
ZraI GAC'GTC
Cuts at: 0 1845 1863
Size: 1845 18
Enzymes that do cut:
AarI AatII AcuI AlfI AlwNI ApoI AvaI BanI
BanII BbsI BcgI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtgZI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI Hin4I HincII MmeI MscI MslI MspA1I
NcoI PasI PflMI PfoI PpuMI PstI PvuII SacI
SanDI SapI SbfI ScaI SexAI SfcI SmlI StuI
StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmtI BsaAI BsaXI BsiEI BsiWI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI
CspCI DraI EagI EciI EcoRV FalI FseI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI XmnI