Restriction map for
pF1KSDB0046
(Linear) (Six-base) MAPSORT of: KIBB0046.seq Check: 3425 from: 1 to: 5925
KIBB0046.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 789 5925
Size: 789 5136
AccI GT'mk_AC
Cuts at: 0 5332 5925
Size: 5332 593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 449 515 1076 1229 1475 1976 2249
Size: 117 332 66 561 153 246 501 273
Cuts at: 2249 3854 3890 4305 4661 5054 5644 5821 5831
Size: 1605 36 415 356 393 590 177 10
Cuts at: 5831 5925
Size: 94
Fragments arranged by size:
1605 590 561 501 415 393 356 332
273 246 177 153 117 94 66 36
10
AflIII A'CryG_T
Cuts at: 0 313 753 1193 5925
Size: 313 440 440 4732
Fragments arranged by size:
4732 440 440 313
AgeI A'CCGG_T
Cuts at: 0 5134 5925
Size: 5134 791
AleI CACnn'nnGTG
Cuts at: 0 1198 3992 4642 5841 5925
Size: 1198 2794 650 1199 84
Fragments arranged by size:
2794 1199 1198 650 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1576 5925
Size: 1576 4349
AlwNI CAG_nnn'CTG
Cuts at: 0 289 904 1999 2473 2488 2664 3511 5359
Size: 289 615 1095 474 15 176 847 1848
Cuts at: 5359 5622 5704 5925
Size: 263 82 221
Fragments arranged by size:
1848 1095 847 615 474 289 263 221
176 82 15
ApoI r'AATT_y
Cuts at: 0 1086 1509 1687 4406 5299 5309 5582 5925
Size: 1086 423 178 2719 893 10 273 343
Fragments arranged by size:
2719 1086 893 423 343 273 178 10
AvaI C'yCGr_G
Cuts at: 0 4076 4337 5925
Size: 4076 261 1588
Fragments arranged by size:
4076 1588 261
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4572 4605 5925
Size: 4572 33 1320
Fragments arranged by size:
4572 1320 33
BamHI G'GATC_C
Cuts at: 0 3168 5925
Size: 3168 2757
BanI G'GyrC_C
Cuts at: 0 2795 5925
Size: 2795 3130
BanII G_rGCy'C
Cuts at: 0 2813 3898 4481 5113 5669 5925
Size: 2813 1085 583 632 556 256
Fragments arranged by size:
2813 1085 632 583 556 256
BbsI GAAGACnn'nnnn_
Cuts at: 0 932 1332 1982 2113 2615 3112 3398 5407
Size: 932 400 650 131 502 497 286 2009
Cuts at: 5407 5925
Size: 518
Fragments arranged by size:
2009 932 650 518 502 497 400 286
131
BbvCI CC'TCA_GC
Cuts at: 0 350 3698 5925
Size: 350 3348 2227
Fragments arranged by size:
3348 2227 350
BciVI GTATCCnnnnn_n'
Cuts at: 0 1647 1723 5925
Size: 1647 76 4202
Fragments arranged by size:
4202 1647 76
BfrBI ATG'CAT
Cuts at: 0 1455 5925
Size: 1455 4470
BglI GCCn_nnn'nGGC
Cuts at: 0 4203 4274 5925
Size: 4203 71 1651
Fragments arranged by size:
4203 1651 71
BglII A'GATC_T
Cuts at: 0 1095 2457 5925
Size: 1095 1362 3468
Fragments arranged by size:
3468 1362 1095
BlpI GC'TnA_GC
Cuts at: 0 2638 5925
Size: 2638 3287
Bme1580I G_kGCm'C
Cuts at: 0 2798 4337 5730 5925
Size: 2798 1539 1393 195
Fragments arranged by size:
2798 1539 1393 195
BmgBI CAC'GTC
Cuts at: 0 1196 2955 5925
Size: 1196 1759 2970
Fragments arranged by size:
2970 1759 1196
BmrI ACTGGGnnnn_n'
Cuts at: 0 734 2361 3899 5385 5925
Size: 734 1627 1538 1486 540
Fragments arranged by size:
1627 1538 1486 734 540
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2065 2373 5925
Size: 2065 308 3552
Fragments arranged by size:
3552 2065 308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 242 1655 2259 2372 4172 4385 4442
Size: 148 94 1413 604 113 1800 213 57
Cuts at: 4442 4923 5512 5683 5925
Size: 481 589 171 242
Fragments arranged by size:
1800 1413 604 589 481 242 213 171
148 113 94 57
Bpu10I CC'TnA_GC
Cuts at: 0 350 3698 4002 5925
Size: 350 3348 304 1923
Fragments arranged by size:
3348 1923 350 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1626 3203 3344 3516 5590 5925
Size: 1626 1577 141 172 2074 335
Fragments arranged by size:
2074 1626 1577 335 172 141
BsaI GGTCTCn'nnnn_
Cuts at: 0 2893 5925
Size: 2893 3032
BsaHI Gr'CG_yC
Cuts at: 0 4129 5925
Size: 4129 1796
BsaWI w'CCGG_w
Cuts at: 0 5134 5925
Size: 5134 791
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4651 4681 4900 4930 5090 5120 5656 5686
Size: 4651 30 219 30 160 30 536 30
Cuts at: 5686 5925
Size: 239
Fragments arranged by size:
4651 536 239 219 160 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 248 1232 1996 3244 3841 3888 3921 4094
Size: 248 984 764 1248 597 47 33 173
Cuts at: 4094 4156 4443 5308 5587 5925
Size: 62 287 865 279 338
Fragments arranged by size:
1248 984 865 764 597 338 287 279
248 173 62 47 33
BseYI C'CCAG_C
Cuts at: 0 1370 2664 3454 4069 4549 4920 5342 5557
Size: 1370 1294 790 615 480 371 422 215
Cuts at: 5557 5787 5925
Size: 230 138
Fragments arranged by size:
1370 1294 790 615 480 422 371 230
215 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 4697 5717 5925
Size: 839 3858 1020 208
Fragments arranged by size:
3858 1020 839 208
BsiHKAI G_wGCw'C
Cuts at: 0 1333 2359 3642 5669 5925
Size: 1333 1026 1283 2027 256
Fragments arranged by size:
2027 1333 1283 1026 256
BsmI GAATG_Cn'
Cuts at: 0 2152 5925
Size: 2152 3773
BsmBI CGTCTCn'nnnn_
Cuts at: 0 230 5925
Size: 230 5695
Bsp1286I G_dGCh'C
Cuts at: 0 1333 2359 2798 2813 3642 3898 4337 4481
Size: 1333 1026 439 15 829 256 439 144
Cuts at: 4481 5113 5669 5730 5925
Size: 632 556 61 195
Fragments arranged by size:
1333 1026 829 632 556 439 439 256
195 144 61 15
BspHI T'CATG_A
Cuts at: 0 1386 5684 5925
Size: 1386 4298 241
Fragments arranged by size:
4298 1386 241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 789 5925
Size: 789 5136
BsrBI CCG'CTC
Cuts at: 0 5252 5925
Size: 5252 673
BsrDI GCAATG_nn'
Cuts at: 0 3377 5815 5925
Size: 3377 2438 110
Fragments arranged by size:
3377 2438 110
BsrFI r'CCGG_y
Cuts at: 0 5134 5925
Size: 5134 791
BsrGI T'GTAC_A
Cuts at: 0 750 5925
Size: 750 5175
BssSI C'ACGA_G
Cuts at: 0 3767 4199 4535 4985 5925
Size: 3767 432 336 450 940
Fragments arranged by size:
3767 940 450 432 336
BstAPI GCAn_nnn'nTGC
Cuts at: 0 823 1038 4060 5925
Size: 823 215 3022 1865
Fragments arranged by size:
3022 1865 823 215
BstEII G'GTnAC_C
Cuts at: 0 3250 3904 5925
Size: 3250 654 2021
Fragments arranged by size:
3250 2021 654
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3567 3580 4632 5925
Size: 3567 13 1052 1293
Fragments arranged by size:
3567 1293 1052 13
BstYI r'GATC_y
Cuts at: 0 1095 1521 2457 3067 3168 5678 5925
Size: 1095 426 936 610 101 2510 247
Fragments arranged by size:
2510 1095 936 610 426 247 101
BstZ17I GTA'TAC
Cuts at: 0 5333 5925
Size: 5333 592
Bsu36I CC'TnA_GG
Cuts at: 0 905 1517 3900 5925
Size: 905 612 2383 2025
Fragments arranged by size:
2383 2025 905 612
BtgI C'CryG_G
Cuts at: 0 3482 4994 5219 5925
Size: 3482 1512 225 706
Fragments arranged by size:
3482 1512 706 225
BtsI GCAGTG_nn'
Cuts at: 0 375 3999 5289 5925
Size: 375 3624 1290 636
Fragments arranged by size:
3624 1290 636 375
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4732 4767 5925
Size: 4732 35 1158
Fragments arranged by size:
4732 1158 35
DraIII CAC_nnn'GTG
Cuts at: 0 2029 5925
Size: 2029 3896
EaeI y'GGCC_r
Cuts at: 0 3711 5222 5243 5925
Size: 3711 1511 21 682
Fragments arranged by size:
3711 1511 682 21
EarI CTCTTCn'nnn_
Cuts at: 0 407 419 3008 3221 3677 3830 5030 5150
Size: 407 12 2589 213 456 153 1200 120
Cuts at: 5150 5285 5925
Size: 135 640
Fragments arranged by size:
2589 1200 640 456 407 213 153 135
120 12
EcoICRI GAG'CTC
Cuts at: 0 5667 5925
Size: 5667 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 148 242 449 515 1076 1229 1475
Size: 117 31 94 207 66 561 153 246
Cuts at: 1475 1655 1976 2249 2259 2372 3854 3890 4172
Size: 180 321 273 10 113 1482 36 282
Cuts at: 4172 4305 4385 4442 4661 4923 5054 5512 5644
Size: 133 80 57 219 262 131 458 132
Cuts at: 5644 5683 5821 5831 5925
Size: 39 138 10 94
Fragments arranged by size:
1482 561 458 321 282 273 262 246
219 207 180 153 138 133 132 131
117 113 94 94 80 66 57 39
36 31 10 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1066 2744 4106 4657 5925
Size: 1066 1678 1362 551 1268
Fragments arranged by size:
1678 1362 1268 1066 551
EcoO109I rG'GnC_Cy
Cuts at: 0 1058 3306 3501 5167 5925
Size: 1058 2248 195 1666 758
Fragments arranged by size:
2248 1666 1058 758 195
EcoRI G'AATT_C
Cuts at: 0 1086 1509 5582 5925
Size: 1086 423 4073 343
Fragments arranged by size:
4073 1086 423 343
EcoRV GAT'ATC
Cuts at: 0 690 2754 3918 4863 5925
Size: 690 2064 1164 945 1062
Fragments arranged by size:
2064 1164 1062 945 690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5649 5925
Size: 5649 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 490 522 898 930 955 987
Size: 184 32 274 32 376 32 25 32
Cuts at: 987 1972 2004 2085 2117 2353 2385 2449 2481
Size: 985 32 81 32 236 32 64 32
Cuts at: 2481 2866 2892 2898 2924 3643 3675 3907 3939
Size: 385 26 6 26 719 32 232 32
Cuts at: 3939 4300 4332 4438 4470 5029 5061 5656 5688
Size: 361 32 106 32 559 32 595 32
Cuts at: 5688 5925
Size: 237
Fragments arranged by size:
985 719 595 559 385 376 361 274
237 236 232 184 106 81 64 32
32 32 32 32 32 32 32 32
32 32 32 32 32 26 26 25
6
HincII GTy'rAC
Cuts at: 0 917 3274 5925
Size: 917 2357 2651
Fragments arranged by size:
2651 2357 917
HindIII A'AGCT_T
Cuts at: 0 2246 2694 5925
Size: 2246 448 3231
Fragments arranged by size:
3231 2246 448
MfeI C'AATT_G
Cuts at: 0 3842 5925
Size: 3842 2083
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 170 410 704 4524 5925
Size: 17 153 240 294 3820 1401
Fragments arranged by size:
3820 1401 294 240 153 17
MscI TGG'CCA
Cuts at: 0 3713 5224 5245 5925
Size: 3713 1511 21 680
Fragments arranged by size:
3713 1511 680 21
MslI CAynn'nnrTG
Cuts at: 0 724 1198 3115 3713 3992 4642 5723 5841
Size: 724 474 1917 598 279 650 1081 118
Cuts at: 5841 5912 5925
Size: 71 13
Fragments arranged by size:
1917 1081 724 650 598 474 279 118
71 13
MspA1I CmG'CkG
Cuts at: 0 220 4040 4258 4632 5557 5823 5925
Size: 220 3820 218 374 925 266 102
Fragments arranged by size:
3820 925 374 266 220 218 102
NcoI C'CATG_G
Cuts at: 0 3482 4994 5219 5925
Size: 3482 1512 225 706
Fragments arranged by size:
3482 1512 706 225
NdeI CA'TA_TG
Cuts at: 0 576 5925
Size: 576 5349
NsiI A_TGCA'T
Cuts at: 0 1457 5925
Size: 1457 4468
NspI r_CATG'y
Cuts at: 0 317 757 801 5925
Size: 317 440 44 5124
Fragments arranged by size:
5124 440 317 44
PciI A'CATG_T
Cuts at: 0 313 753 5925
Size: 313 440 5172
Fragments arranged by size:
5172 440 313
PflMI CCAn_nnn'nTGG
Cuts at: 0 2226 2664 2770 3333 3483 5925
Size: 2226 438 106 563 150 2442
Fragments arranged by size:
2442 2226 563 438 150 106
PfoI T'CCnGG_A
Cuts at: 0 3743 4458 5660 5925
Size: 3743 715 1202 265
Fragments arranged by size:
3743 1202 715 265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1593 1625 5925
Size: 1593 32 4300
Fragments arranged by size:
4300 1593 32
PpuMI rG'GwC_Cy
Cuts at: 0 1058 3306 3501 5925
Size: 1058 2248 195 2424
Fragments arranged by size:
2424 2248 1058 195
PstI C_TGCA'G
Cuts at: 0 884 1043 2478 5925
Size: 884 159 1435 3447
Fragments arranged by size:
3447 1435 884 159
PvuII CAG'CTG
Cuts at: 0 220 4258 5557 5823 5925
Size: 220 4038 1299 266 102
Fragments arranged by size:
4038 1299 266 220 102
SacI G_AGCT'C
Cuts at: 0 5669 5925
Size: 5669 256
SanDI GG'GwC_CC
Cuts at: 0 3501 5925
Size: 3501 2424
SapI GCTCTTCn'nnn_
Cuts at: 0 3830 5150 5925
Size: 3830 1320 775
Fragments arranged by size:
3830 1320 775
ScaI AGT'ACT
Cuts at: 0 2826 3414 5452 5925
Size: 2826 588 2038 473
Fragments arranged by size:
2826 2038 588 473
SfcI C'TryA_G
Cuts at: 0 880 1039 2474 2489 4722 5087 5273 5925
Size: 880 159 1435 15 2233 365 186 652
Fragments arranged by size:
2233 1435 880 652 365 186 159 15
SgrAI Cr'CCGG_yG
Cuts at: 0 5134 5925
Size: 5134 791
SmlI C'TyrA_G
Cuts at: 0 1605 3218 3359 3495 5569 5925
Size: 1605 1613 141 136 2074 356
Fragments arranged by size:
2074 1613 1605 356 141 136
StuI AGG'CCT
Cuts at: 0 3439 4160 5925
Size: 3439 721 1765
Fragments arranged by size:
3439 1765 721
StyI C'CwwG_G
Cuts at: 0 486 2109 2426 3482 3935 4161 4271 4781
Size: 486 1623 317 1056 453 226 110 510
Cuts at: 4781 4994 5219 5925
Size: 213 225 706
Fragments arranged by size:
1623 1056 706 510 486 453 317 226
225 213 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 986 3198 4360 5835 5925
Size: 986 2212 1162 1475 90
Fragments arranged by size:
2212 1475 1162 986 90
TatI w'GTAC_w
Cuts at: 0 750 1743 2824 3412 3562 4718 5450 5925
Size: 750 993 1081 588 150 1156 732 475
Fragments arranged by size:
1156 1081 993 750 732 588 475 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 354 371 386 403 982 1014 1325 1346
Size: 354 17 15 17 579 32 311 21
Cuts at: 1346 1357 1378 4965 4997 5090 5122 5466 5498
Size: 11 21 3587 32 93 32 344 32
Cuts at: 5498 5925
Size: 427
Fragments arranged by size:
3587 579 427 354 344 311 93 32
32 32 32 21 21 17 17 15
11
Tth111I GACn'n_nGTC
Cuts at: 0 3111 5925
Size: 3111 2814
XbaI T'CTAG_A
Cuts at: 0 108 5925
Size: 108 5817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4423 5238 5925
Size: 4423 815 687
Fragments arranged by size:
4423 815 687
XmnI GAAnn'nnTTC
Cuts at: 0 5452 5925
Size: 5452 473
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AleI AlfI AlwNI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PciI PflMI PfoI
PpiI PpuMI PstI PvuII SacI SanDI SapI ScaI
SfcI SgrAI SmlI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BbeI BcgI BclI
BmtI BsaAI BsaBI BsiEI BsiWI BspEI BssHII BstBI
BtgZI ClaI DraI DrdI EagI EciI FseI FspI
FspAI HaeII HpaI KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI SgfI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XhoI XmaI ZraI