Restriction map for
pF1KSDB0042
(Linear) (Six-base) MAPSORT of: KIBB0042.seq Check: 8381 from: 1 to: 2013
KIBB0042.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 1748 2013
Size: 1748 265
Acc65I G'GTAC_C
Cuts at: 0 292 2013
Size: 292 1721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1111 1622 2013
Size: 788 323 511 391
Fragments arranged by size:
788 511 391 323
AfeI AGC'GCT
Cuts at: 0 1174 2013
Size: 1174 839
AleI CACnn'nnGTG
Cuts at: 0 173 2013
Size: 173 1840
AlwNI CAG_nnn'CTG
Cuts at: 0 1116 2004 2013
Size: 1116 888 9
Fragments arranged by size:
1116 888 9
BanI G'GyrC_C
Cuts at: 0 194 292 2013
Size: 194 98 1721
Fragments arranged by size:
1721 194 98
BanII G_rGCy'C
Cuts at: 0 536 1583 1817 2013
Size: 536 1047 234 196
Fragments arranged by size:
1047 536 234 196
BbsI GAAGACnn'nnnn_
Cuts at: 0 938 1198 1720 2013
Size: 938 260 522 293
Fragments arranged by size:
938 522 293 260
BbvCI CC'TCA_GC
Cuts at: 0 1282 2013
Size: 1282 731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1330 1364 1455 1489 2013
Size: 1330 34 91 34 524
Fragments arranged by size:
1330 524 91 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1651 2013
Size: 1651 362
BglII A'GATC_T
Cuts at: 0 828 1770 2013
Size: 828 942 243
Fragments arranged by size:
942 828 243
Bme1580I G_kGCm'C
Cuts at: 0 192 2013
Size: 192 1821
BmrI ACTGGGnnnn_n'
Cuts at: 0 353 530 1874 1916 2013
Size: 353 177 1344 42 97
Fragments arranged by size:
1344 353 177 97 42
BmtI G_CTAG'C
Cuts at: 0 973 2013
Size: 973 1040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 665 2013
Size: 665 1348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 318 621 749 1567 2013
Size: 151 167 303 128 818 446
Fragments arranged by size:
818 446 303 167 151 128
Bpu10I CC'TnA_GC
Cuts at: 0 1282 2013
Size: 1282 731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 2013
Size: 689 1324
BsaBI GATnn'nnATC
Cuts at: 0 1027 2013
Size: 1027 986
BsaWI w'CCGG_w
Cuts at: 0 83 1547 2013
Size: 83 1464 466
Fragments arranged by size:
1464 466 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 449 479 2013
Size: 449 30 1534
Fragments arranged by size:
1534 449 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 987 2013
Size: 49 938 1026
Fragments arranged by size:
1026 938 49
BseYI C'CCAG_C
Cuts at: 0 1518 1851 2004 2013
Size: 1518 333 153 9
Fragments arranged by size:
1518 333 153 9
BsiEI CG_ry'CG
Cuts at: 0 1013 2013
Size: 1013 1000
BsiHKAI G_wGCw'C
Cuts at: 0 1583 2013
Size: 1583 430
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1270 2013
Size: 1270 743
Bsp1286I G_dGCh'C
Cuts at: 0 192 536 1583 1817 2013
Size: 192 344 1047 234 196
Fragments arranged by size:
1047 344 234 196 192
BspEI T'CCGG_A
Cuts at: 0 83 2013
Size: 83 1930
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 438 2013
Size: 438 1575
BsrBI CCG'CTC
Cuts at: 0 522 2013
Size: 522 1491
BsrDI GCAATG_nn'
Cuts at: 0 294 1425 1833 2013
Size: 294 1131 408 180
Fragments arranged by size:
1131 408 294 180
BstAPI GCAn_nnn'nTGC
Cuts at: 0 311 1935 2013
Size: 311 1624 78
Fragments arranged by size:
1624 311 78
BstEII G'GTnAC_C
Cuts at: 0 1543 2013
Size: 1543 470
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1955 2013
Size: 1955 58
BstYI r'GATC_y
Cuts at: 0 828 1292 1385 1770 2013
Size: 828 464 93 385 243
Fragments arranged by size:
828 464 385 243 93
Bsu36I CC'TnA_GG
Cuts at: 0 796 2013
Size: 796 1217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 943 2013
Size: 943 1070
BtsI GCAGTG_nn'
Cuts at: 0 440 2013
Size: 440 1573
DraIII CAC_nnn'GTG
Cuts at: 0 258 948 2013
Size: 258 690 1065
Fragments arranged by size:
1065 690 258
DrdI GACnn_nn'nnGTC
Cuts at: 0 1648 2013
Size: 1648 365
EaeI y'GGCC_r
Cuts at: 0 496 1820 1991 2013
Size: 496 1324 171 22
Fragments arranged by size:
1324 496 171 22
EarI CTCTTCn'nnn_
Cuts at: 0 71 908 2013
Size: 71 837 1105
Fragments arranged by size:
1105 837 71
EcoICRI GAG'CTC
Cuts at: 0 1581 2013
Size: 1581 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 318 621 749 788 1111 1567 1622
Size: 151 167 303 128 39 323 456 55
Cuts at: 1622 2013
Size: 391
Fragments arranged by size:
456 391 323 303 167 151 128 55
39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1375 1538 2013
Size: 1375 163 475
Fragments arranged by size:
1375 475 163
EcoO109I rG'GnC_Cy
Cuts at: 0 1319 2013
Size: 1319 694
HaeII r_GCGC'y
Cuts at: 0 1176 2013
Size: 1176 837
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 449 481 585 617 619 651 678 710
Size: 449 32 104 32 2 32 27 32
Cuts at: 710 1707 1739 2013
Size: 997 32 274
Fragments arranged by size:
997 449 274 104 32 32 32 32
32 27 2
HindIII A'AGCT_T
Cuts at: 0 1405 2013
Size: 1405 608
KpnI G_GTAC'C
Cuts at: 0 296 2013
Size: 296 1717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1518 2013
Size: 1518 495
MscI TGG'CCA
Cuts at: 0 498 1822 1993 2013
Size: 498 1324 171 20
Fragments arranged by size:
1324 498 171 20
MslI CAynn'nnrTG
Cuts at: 0 173 857 1063 2013
Size: 173 684 206 950
Fragments arranged by size:
950 684 206 173
MspA1I CmG'CkG
Cuts at: 0 619 1146 2013
Size: 619 527 867
Fragments arranged by size:
867 619 527
NdeI CA'TA_TG
Cuts at: 0 1185 1781 2013
Size: 1185 596 232
Fragments arranged by size:
1185 596 232
NheI G'CTAG_C
Cuts at: 0 969 2013
Size: 969 1044
NspI r_CATG'y
Cuts at: 0 987 2013
Size: 987 1026
PasI CC'CwG_GG
Cuts at: 0 1948 2013
Size: 1948 65
PflMI CCAn_nnn'nTGG
Cuts at: 0 1116 1146 2013
Size: 1116 30 867
Fragments arranged by size:
1116 867 30
PfoI T'CCnGG_A
Cuts at: 0 637 1399 2013
Size: 637 762 614
Fragments arranged by size:
762 637 614
PpuMI rG'GwC_Cy
Cuts at: 0 1319 2013
Size: 1319 694
PstI C_TGCA'G
Cuts at: 0 435 1328 1976 2013
Size: 435 893 648 37
Fragments arranged by size:
893 648 435 37
PvuI CG_AT'CG
Cuts at: 0 1013 2013
Size: 1013 1000
PvuII CAG'CTG
Cuts at: 0 619 1146 2013
Size: 619 527 867
Fragments arranged by size:
867 619 527
SacI G_AGCT'C
Cuts at: 0 1583 2013
Size: 1583 430
SexAI A'CCwGG_T
Cuts at: 0 254 2013
Size: 254 1759
SfcI C'TryA_G
Cuts at: 0 64 171 431 1032 1324 1774 1972 2013
Size: 64 107 260 601 292 450 198 41
Fragments arranged by size:
601 450 292 260 198 107 64 41
SmlI C'TyrA_G
Cuts at: 0 704 2013
Size: 704 1309
SphI G_CATG'C
Cuts at: 0 987 2013
Size: 987 1026
StuI AGG'CCT
Cuts at: 0 801 896 2013
Size: 801 95 1117
Fragments arranged by size:
1117 801 95
StyI C'CwwG_G
Cuts at: 0 317 866 1221 1371 2013
Size: 317 549 355 150 642
Fragments arranged by size:
642 549 355 317 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 475 1843 2013
Size: 475 1368 170
Fragments arranged by size:
1368 475 170
TatI w'GTAC_w
Cuts at: 0 809 2013
Size: 809 1204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1245 1277 2013
Size: 1245 32 736
Fragments arranged by size:
1245 736 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2001 2013
Size: 2001 12
Enzymes that do cut:
AccI Acc65I AcuI AfeI AleI AlwNI BanI BanII
BbsI BbvCI BcgI BciVI BglII Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BstAPI BstEII BstXI BstYI Bsu36I BtgZI BtsI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I
NdeI NheI NspI PasI PflMI PfoI PpuMI PstI
PvuI PvuII SacI SexAI SfcI SmlI SphI StuI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BclI BfrBI BglI BlpI
BmgBI BsaI BsaAI BsaHI BsgI BsiWI BsmI BspHI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I BtgI ClaI
CspCI DraI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KasI MfeI MluI NaeI
NarI NcoI NgoMIV NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI